Team:Paris/constructions

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<span/ id="bottom">[https://2009.igem.org/ iGEM ] > [[Team:Paris#top | Paris]] > [[Team:Paris/Freezer#top | Freezer]] > [[Team:Paris/constructions#bottom | Constructions in progress]]
{{Template:Paris2009}}
{{Template:Paris2009}}
{{Template:Paris2009_menu}}
{{Template:Paris2009_menu}}
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==Freezer==
 
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<center>
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== Constructions ==
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[[Team:Paris/Freezer_Primers#Freezer | Primers]] -
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<html>
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[[Team:Paris/Freezer_PCR_products#Freezer | PCR products]] - 
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<style type="text/css">
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[[Team:Paris/Freezer_Plasmids#Freezer | Plasmids]] -
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#left-side {
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[[Team:Paris/Freezer_Digestion_Products#Freezer | Digestion Products]] -
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    position: absolute;
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[[Team:Paris/Freezer_Unexpected_digestion_results#Freezer | Unexpected digestion results]] -
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    height: 23px;
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[[Team:Paris/Freezer_Strains_(Glycerol stock)#Freezer | Strains (Glycerol stock)]] - [[Team:Paris/constructions#Freezer | Construction in progress]]
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    background: url(https://static.igem.org/mediawiki/2009/4/40/Right_menu_paris.png);
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}
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</style>
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<div id="left-side"></div>
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<div id="middle-side"><center>
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<a class="menu_sub"href="https://2009.igem.org/Team:Paris/Freezer#bottom"> Main </a>|
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<a class="menu_sub" href="https://2009.igem.org/Team:Paris/Freezer_PCR_products#bottom"> PCR Products</a>|
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<a class="menu_sub"href="https://2009.igem.org/Team:Paris/Freezer_Plasmids#bottom"> Plasmids</a>|
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<a class="menu_sub"href="https://2009.igem.org/Team:Paris/Freezer_Digestion_Products#bottom"> Digestion Products</a>|
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<a class="menu_sub"href="https://2009.igem.org/Team:Paris/Freezer_Strains_(Glycerol_stock)#bottom"> Strains (Glycerol stock)</a>|
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<a class="menu_sub_active"href="https://2009.igem.org/Team:Paris/constructions#bottom"> Constructions in progress</a>
</center>
</center>
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</div>
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<div id="right-side"></div>
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{|
{|
|- style="background: #c5c5c5;"
|- style="background: #c5c5c5;"
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|width=10% style="background: #FF0066;"| Chloranphenicol
|width=10% style="background: #FF0066;"| Chloranphenicol
|}
|}
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<div id="paris_content_boxtop">
 
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== Constructions ==
 
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|- style="background: #FFCC00; text-align: center"
|- style="background: #FFCC00; text-align: center"
|CC!
|CC!
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|((rbs - jun/foss) :: ter) in [http://partsregistry.org/Part:pSB2K3 pSB2K3]
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|((???autotransporter??? - jun/foss) :: ter) in [http://partsregistry.org/Part:pSB2K3 pSB2K3]
| BBa_??? :: [http://partsregistry.org/Part:BBa_B0014 BBa_B0014] in [http://partsregistry.org/Part:pSB2K3 pSB2K3]
| BBa_??? :: [http://partsregistry.org/Part:BBa_B0014 BBa_B0014] in [http://partsregistry.org/Part:pSB2K3 pSB2K3]
-
|jun/foss has to be synthetized (10/08/09)
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|jun/foss has to be synthetized & autotransporter to be found!!!! (10/08/09)
|??
|??
|BBa_????
|BBa_????
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|- style="background: #ccccff; text-align: center"
|- style="background: #ccccff; text-align: center"
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|GC
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|GC <span/ id="ptet">
|((Plac :: rbs-tetR) :: rbs-tolRII) in pSB2A3
|((Plac :: rbs-tetR) :: rbs-tolRII) in pSB2A3
|BBa_blabla :: BBa_tralala in truc
|BBa_blabla :: BBa_tralala in truc
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|
|
|??
|??
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|BBa_????
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|[http://partsregistry.org/wiki/index.php?title=Part:BBa_K257015 BBa_K257015]
|- style="background: #ccff00; text-align: center"
|- style="background: #ccff00; text-align: center"
|CC
|CC
|(ter :: ((Plac :: rbs-tetR) :: ter)) in [http://partsregistry.org/Part:pSB1A3 pSB1A3]
|(ter :: ((Plac :: rbs-tetR) :: ter)) in [http://partsregistry.org/Part:pSB1A3 pSB1A3]
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|[http://partsregistry.org/Part:BBa_B0014 BBa_B0014] :: BBa_??? in [http://partsregistry.org/Part:pSB1A3 pSB1A3]
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|[http://partsregistry.org/Part:BBa_B0014 BBa_B0014] :: [http://partsregistry.org/wiki/index.php?title=Part:BBa_K257015 BBa_K257015] in [http://partsregistry.org/Part:pSB1A3 pSB1A3]
|
|
|??
|??
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|CL
|CL
|(rbs - OmpAsignal :: TolR II)
|(rbs - OmpAsignal :: TolR II)
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|BBa_????
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|[http://partsregistry.org/wiki/index.php?title=Part:BBa_K257006 BBa_K257006] ::[http://partsregistry.org/wiki/index.php?title=Part:BBa_K257005 BBa_K257005] in [http://partsregistry.org/Part:pSB1A3 pSB1A3]
|OmpA signal and TolRII amplified, but neither digested nor purified (10/08/09)
|OmpA signal and TolRII amplified, but neither digested nor purified (10/08/09)
|??
|??
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|- style="background: #00FF00; text-align: center"
|- style="background: #00FF00; text-align: center"
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|CC
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|CC <span/ id="tolr">
|(Ptet :: (rbs - OmpAsignal :: TolRII)) in [http://partsregistry.org/Part:pSB1A3 pSB1A3]
|(Ptet :: (rbs - OmpAsignal :: TolRII)) in [http://partsregistry.org/Part:pSB1A3 pSB1A3]
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|[http://partsregistry.org/Part:BBa_R0040 Bba_R0040] :: BBa_??? in [http://partsregistry.org/Part:pSB1A3 pSB1A3]
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|[http://partsregistry.org/Part:BBa_R0040 Bba_R0040] :: [http://partsregistry.org/wiki/index.php?title=Part:BBa_K257006 BBa_K257006] ::[http://partsregistry.org/wiki/index.php?title=Part:BBa_K257005 BBa_K257005] in [http://partsregistry.org/Part:pSB1A3 pSB1A3]
|Ptet digested and purified (10/08/09)
|Ptet digested and purified (10/08/09)
|??
|??
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|- style="background: #006600; text-align: center; color:white;"
|- style="background: #006600; text-align: center; color:white;"
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|CC
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|CC <span/ id="tolr2">
|((Ptet :: (rbs - OmpAsignal :: TolRIIP)) :: (ter :: ((Plac :: rbs-tetR) :: ter))) in [http://partsregistry.org/Part:pSB1A3 pSB1A3]
|((Ptet :: (rbs - OmpAsignal :: TolRIIP)) :: (ter :: ((Plac :: rbs-tetR) :: ter))) in [http://partsregistry.org/Part:pSB1A3 pSB1A3]
|BBa_??? :: BBa_??? in [http://partsregistry.org/Part:pSB1A3 pSB1A3]
|BBa_??? :: BBa_??? in [http://partsregistry.org/Part:pSB1A3 pSB1A3]

Latest revision as of 21:51, 21 October 2009

iGEM > Paris > Freezer > Constructions in progress


Constructions


Color code for sticker
Vector Insert PCR product Plasmid Strains Kanamycin Ampicilin Chloranphenicol


  • Plasmid A


Designer Parts description Corresponding BioBricks Status (last update) Plasmid # Registry
CC (Pbad :: rbs - lacI - LVA) in [http://partsregistry.org/Part:pSB1A3 pSB1A3]???Pbad is on PSB2K3 [http://partsregistry.org/Part:BBa_I0500 Bba_I0500] :: [http://partsregistry.org/Part:BBa_J24679 BBa_J24679] in [http://partsregistry.org/Part:pSB2K3 pSB2K3] pSB1A3 transformed, digested, PBad transformed and not digested well- LacI transformed and digested (10/08/09) ?? BBa_????
CC (Pbad :: rbs - lacI - LVA) :: ter in [http://partsregistry.org/Part:pSB1A3 pSB1A3] BBa_??? :: [http://partsregistry.org/Part:BBa_B0014 BBa_B0014] in [http://partsregistry.org/Part:pSB2K3 pSB2K3] ter transformed, digested and purified (10/08/09) ?? BBa_????
CC ((Pbad :: rbs - lacI - LVA) :: RFP) in [http://partsregistry.org/Part:pSB1A3 pSB1A3] BBa_??? :: [http://partsregistry.org/wiki/index.php?title=Part:BBa_K093005 BBa_K093005] in [http://partsregistry.org/Part:pSB2K3 pSB2K3] pSB2K3 transformed and digested, RFP : primers designed but not yet ordered (10/08/09) ?? BBa_????


CC (((Pbad :: rbs - lacI - LVA) :: RFP) :: ter) in [http://partsregistry.org/Part:pSB1A3 pSB1A3] BBa_??? :: [http://partsregistry.org/Part:BBa_B0014 BBa_B0014] in [http://partsregistry.org/Part:pSB2K3 pSB2K3] ?? BBa_????


CC (OmpA Linker :: g3p) in [http://partsregistry.org/Part:pSB2K3 pSB2K3] [http://partsregistry.org/Part:BBa_K103006 BBa_K103006] :: BBa_??? in [http://partsregistry.org/Part:pSB2K3 pSB2K3] OmpA linker standardisé - OmpA amplified, not purified - Same for g3p (10/08/09) ?? BBa_????
(g3p :: clyA) in [http://partsregistry.org/Part:pSB2K3 pSB2K3] BBa_??? :: BBa_??? in [http://partsregistry.org/Part:pSB2K3 pSB2K3] autre possibilité (souf)- 1 primer missing for C term fusion with clyA (10/08/09) ?? BBa_????
CC! ((OmpA Linker :: g3p) :: ter) in [http://partsregistry.org/Part:pSB2K3 pSB2K3] BBa_??? :: [http://partsregistry.org/Part:BBa_B0014 BBa_B0014] in [http://partsregistry.org/Part:pSB2K3 pSB2K3] ?? BBa_????
CC! ((???autotransporter??? - jun/foss) :: ter) in [http://partsregistry.org/Part:pSB2K3 pSB2K3] BBa_??? :: [http://partsregistry.org/Part:BBa_B0014 BBa_B0014] in [http://partsregistry.org/Part:pSB2K3 pSB2K3] jun/foss has to be synthetized & autotransporter to be found!!!! (10/08/09) ?? BBa_????
CC! ((rbs - fecA) :: ((rbs - jun/foss) :: ter)) in [http://partsregistry.org/Part:pSB2K3 pSB2K3] BBa_??? :: BBa_??? in [http://partsregistry.org/Part:pSB2K3 pSB2K3] FecA : oligo ordered but not received yet (10/08/09) ?? BBa_????
CC! ((rbs - fecA) :: ((Ompa Linker :: g3p) :: ter)) in [http://partsregistry.org/Part:pSB2K3 pSB2K3] BBa_??? :: BBa_??? in [http://partsregistry.org/Part:pSB2K3 pSB2K3] ?? BBa_????
CC ((rbs - ClyA :: RFP) in [http://partsregistry.org/Part:pSB2K3 pSB2K3] BBa_??? :: BBa_???? in [http://partsregistry.org/Part:pSB2K3 pSB2K3] modif RFP (souf)- some troubles with amplification of clyA fusion C term (10/08/09) ?? BBa_????
CC ((rbs-RFP :: clyA) in [http://partsregistry.org/Part:pSB2K3 pSB2K3] BBa_??? :: BBa_???? in [http://partsregistry.org/Part:pSB2K3 pSB2K3] modif RFP (souf) - fusion N term de cly A (autre possibilité)- we still miss 1 primer for clyA N term fusion (10/08/09) ?? BBa_????
CC! ((rbs - ClyA :: RFP)) :: (((rbs - fecA) :: (rbs - jun/foss)) :: ter)) in [http://partsregistry.org/Part:pSB2K3 pSB2K3] BBa_??? :: BBa_???? in [http://partsregistry.org/Part:pSB2K3 pSB2K3] modif RFP (souf) ?? BBa_????
CC! ((rbs - ClyA :: RFP) :: (((rbs - fecA) :: (OmpA linker :: g3p)) :: ter)) in [http://partsregistry.org/Part:pSB2K3 pSB2K3] BBa_??? :: BBa_???? in [http://partsregistry.org/Part:pSB2K3 pSB2K3] modif RFP (souf) ?? BBa_????
CC! (Pbad :: ((rbs - ClyA :: RFP) :: (((rbs - fecA) :: (OmpA linker :: g3p)) :: ter))) in [http://partsregistry.org/Part:pSB2K3 pSB2K3] [http://partsregistry.org/Part:BBa_I0500 Bba_I0500] :: BBa_???? in [http://partsregistry.org/Part:pSB2K3 pSB2K3] ?? BBa_????
CC! (Pbad :: ((rbs - ClyA :: RFP) :: (((rbs - fecA) :: (rbs - jun/foss)) :: ter))) in [http://partsregistry.org/Part:pSB2K3 pSB2K3] BBa_??? :: BBa_???? in [http://partsregistry.org/Part:pSB2K3 pSB2K3] ?? BBa_????
CC! ((Pbad :: ((rbs - ClyA :: RFP) :: (((rbs - fecA) :: (OmpA linker :: g3p)) :: ter))) :: ((Pbad :: rbs - lacI - LVA) :: ter)) in [http://partsregistry.org/Part:pSB2K3 pSB2K3] BBa_??? :: BBa_???? in [http://partsregistry.org/Part:pSB2K3 pSB2K3] 2*Pbad et 2*ter?? (souf) ?? BBa_????
CC! ((Pbad :: ((rbs - ClyA :: RFP) :: (((rbs - fecA) :: (rbs - jun/foss)) :: ter))) :: ((Pbad :: rbs - lacI - LVA) :: ter)) in [http://partsregistry.org/Part:pSB2K3 pSB2K3] BBa_??? :: BBa_???? in [http://partsregistry.org/Part:pSB2K3 pSB2K3] ?? BBa_????



  • Plasmid B


Designer Parts description Corresponding BioBricks Status (last update) Plasmid # Registry
GC ((Plac :: rbs-tetR) :: rbs-tolRII) in pSB2A3 BBa_blabla :: BBa_tralala in truc Planning / oligo desgned or ordered / PCR in progress / cloned / sequencing... (10/08/09) 12 BBa_pouet pouet
CC (Plac :: rbs-tetR) in [http://partsregistry.org/Part:pSB1A3 pSB1A3] [http://partsregistry.org/Part:BBa_I14032 Bba_I14032] :: [http://partsregistry.org/Part:BBa_K136050 BBa_K136050] in [http://partsregistry.org/Part:pSB1A3 pSB1A3] both have been digested and purified, ligation in progress (10/08/09) ?? BBa_????
CC ((Plac :: rbs-tetR) :: ter) in [http://partsregistry.org/Part:pSB1A3 pSB1A3] BBa_???? :: [http://partsregistry.org/Part:BBa_B0014 BBa_B0014] in [http://partsregistry.org/Part:pSB1A3 pSB1A3] ?? [http://partsregistry.org/wiki/index.php?title=Part:BBa_K257015 BBa_K257015]
CC (ter :: ((Plac :: rbs-tetR) :: ter)) in [http://partsregistry.org/Part:pSB1A3 pSB1A3] [http://partsregistry.org/Part:BBa_B0014 BBa_B0014] :: [http://partsregistry.org/wiki/index.php?title=Part:BBa_K257015 BBa_K257015] in [http://partsregistry.org/Part:pSB1A3 pSB1A3] ?? BBa_????
CL (rbs - OmpAsignal :: TolR II) [http://partsregistry.org/wiki/index.php?title=Part:BBa_K257006 BBa_K257006] ::[http://partsregistry.org/wiki/index.php?title=Part:BBa_K257005 BBa_K257005] in [http://partsregistry.org/Part:pSB1A3 pSB1A3] OmpA signal and TolRII amplified, but neither digested nor purified (10/08/09) ?? BBa_????
CC (Ptet :: (rbs - OmpAsignal :: TolRII)) in [http://partsregistry.org/Part:pSB1A3 pSB1A3] [http://partsregistry.org/Part:BBa_R0040 Bba_R0040] :: [http://partsregistry.org/wiki/index.php?title=Part:BBa_K257006 BBa_K257006] ::[http://partsregistry.org/wiki/index.php?title=Part:BBa_K257005 BBa_K257005] in [http://partsregistry.org/Part:pSB1A3 pSB1A3] Ptet digested and purified (10/08/09) ?? BBa_????
CC (rbs :: EYFP) in [http://partsregistry.org/Part:pSB1A3 pSB1A3] BBa_??? :: [http://partsregistry.org/wiki/index.php?title=Part:BBa_E0030 BBa_E0030] in [http://partsregistry.org/Part:pSB1A3 pSB1A3] y a t-il vraiment 2 biobricks ou bien met-on le RBS direct dans des oligos pour RFP? (souf) biobrick to find or primers to design (10/08/09) ?? BBa_????
CC (Ptet :: (rbs :: EYFP)) in [http://partsregistry.org/Part:pSB1A3 pSB1A3] [http://partsregistry.org/Part:BBa_R0040 Bba_R0040] :: BBa_??? in [http://partsregistry.org/Part:pSB1A3 pSB1A3] ?? BBa_????
CC ((Ptet :: (rbs :: EYFP)) :: (ter :: ((Plac :: rbs-tetR) :: ter))) in [http://partsregistry.org/Part:pSB1A3 pSB1A3] BBa_??? :: BBa_??? in [http://partsregistry.org/Part:pSB1A3 pSB1A3] ?? BBa_????
CC ((Ptet :: (rbs - OmpAsignal :: TolRIIP)) :: (ter :: ((Plac :: rbs-tetR) :: ter))) in [http://partsregistry.org/Part:pSB1A3 pSB1A3] BBa_??? :: BBa_??? in [http://partsregistry.org/Part:pSB1A3 pSB1A3] ?? BBa_????


  • Plasmid C


Designer Parts description Corresponding BioBricks Status (last update) Plasmid # Registry
CC (rbs-FecI :: rbs-FecR) in [http://partsregistry.org/Part:pSB2K3 pSB2K3] BBa_??? :: BBa_??? primers ordered but not received yet (10/08/09) ?? BBa_????


CC PfecA in [http://partsregistry.org/Part:BBa_J61002 Bba_J61002] BBa_??? vector digested and purified, Pfec A amplified but not digested (purified?) (10/08/09) ?? BBa_????

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