"
Team:UNICAMP-Brazil/Notebooks/August 31
From 2009.igem.org
(→YeastGuard) |
|||
| (4 intermediate revisions not shown) | |||
| Line 9: | Line 9: | ||
=====Quantification===== | =====Quantification===== | ||
| - | We have verified the concentration of RNA we got in the RNA extractions from the ''G. gallus'' organs. The following table shows the results. | + | *<p style=”text-align:justify;”>We have verified the concentration of RNA we got in the RNA extractions from the ''G. gallus'' organs. The following table shows the results.</p> |
{| style="color:black; background-color:white;font-style:none;" cellpadding="2" cellspacing="0" border="1" align="center" | {| style="color:black; background-color:white;font-style:none;" cellpadding="2" cellspacing="0" border="1" align="center" | ||
| Line 37: | Line 37: | ||
!Oviduct 4 !!3568.66 !!1.84 !!1.12 | !Oviduct 4 !!3568.66 !!1.84 !!1.12 | ||
|} | |} | ||
| - | The concentration values are ok, but the ratios 260/280 and 260/230 are not that good. Let’s find out what we see in the agarose gel. | + | *<p style=”text-align:justify;”>The concentration values are ok, but the ratios 260/280 and 260/230 are not that good. Let’s find out what we see in the agarose gel.</p> |
''Raíssa'' | ''Raíssa'' | ||
=====Agarose gel===== | =====Agarose gel===== | ||
| - | We have chosen the best samples from each organ to verify the quality in the gel. The samples are: Blood1, Blood3, Spleen1 and Oviduct2. We had problems with our UV transilluminator and didn’t manage to take pictures of the gel. =[ | + | *<p style=”text-align:justify;”>We have chosen the best samples from each organ to verify the quality in the gel. The samples are: Blood1, Blood3, Spleen1 and Oviduct2. We had problems with our UV transilluminator and didn’t manage to take pictures of the gel. =[</p> |
| - | But we are going to tell you what we found! The bands were there, especially in the spleen and oviduct samples, not perfect, but we bet they are ok for cDNA synthesis. | + | *<p style=”text-align:justify;”>But we are going to tell you what we found! The bands were there, especially in the spleen and oviduct samples, not perfect, but we bet they are ok for cDNA synthesis.</p> |
''Taís'' | ''Taís'' | ||
| - | ====Terminator and RBS+CFP==== | + | ====Terminator biobrick and RBS+CFP ligation==== |
| - | + | *<p style=”text-align:justify;”>We verified using electrophoresis, the digestion products of both parts digested yesterday (Terminator and RBS+CFP).</p> | |
| - | The gel showed we had no success with the digestions. Two | + | *<p style=”text-align:justify;”>The gel showed we had no success with the digestions. Two inespecific bands appeared.</p> |
| + | |||
| + | [[Image:20090905_dig_CFP_RBS_END_05-09-09 possivelgel.JPG|100px|center|]] | ||
''Wesley'' | ''Wesley'' | ||
{{:Team:UNICAMP-Brazil/inc_rodape}} | {{:Team:UNICAMP-Brazil/inc_rodape}} | ||
Latest revision as of 02:00, 22 October 2009
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
