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Team:UNICAMP-Brazil/Notebooks/September 1
From 2009.igem.org
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====''Gallus gallus'' cDNA synthesis==== | ====''Gallus gallus'' cDNA synthesis==== | ||
| - | We decided to make cDNA synthesis of the 4 samples of RNA (Blood1, Blood3, Spleen1 and Oviduct2). We used both pDN6 and oligodT primers for the synthesis. | + | *<p style=”text-align:justify;”>We decided to make cDNA synthesis of the 4 samples of RNA (Blood1, Blood3, Spleen1 and Oviduct2). We used both pDN6 and oligodT primers for the synthesis.</p> |
''Taís'' | ''Taís'' | ||
| - | ====RBS+CFP | + | ====RBS+CFP Digestion==== |
| - | We performed a new digestion of the plasmids containing the ligation RBS+CFP. We followed [https://2009.igem.org/Team:UNICAMP-Brazil/Protocols/Restriction_reaction Protocol 14]. The digestion lasted four hours and we used less plasmid comparing to the first digestion. | + | *<p style=”text-align:justify;”>We performed a new digestion of the plasmids containing the ligation RBS+CFP. We followed [https://2009.igem.org/Team:UNICAMP-Brazil/Protocols/Restriction_reaction Protocol 14]. The digestion lasted four hours and we used less plasmid comparing to the first digestion.</p> |
''Wesley'' | ''Wesley'' | ||
{{:Team:UNICAMP-Brazil/inc_rodape}} | {{:Team:UNICAMP-Brazil/inc_rodape}} | ||
Latest revision as of 02:01, 22 October 2009
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