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Team:UNICAMP-Brazil/Notebooks/September 10
From 2009.igem.org
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====New biobricks in biobrick format==== | ====New biobricks in biobrick format==== | ||
| - | In order to make the new biobricks, the 4 new parts that were digested with the enzymes | + | *<p style=”text-align:justify;”>In order to make the new biobricks, the 4 new parts that were digested with the enzymes ''Xba''I and ''Spe''I must be ligated to the biofusion vector, that was also already digested with the same enzymes. So today we prepared the ligation reactions (according to [https://2009.igem.org/Team:UNICAMP-Brazil/Protocols/T4_DNA_Ligase Protocol 11]) that will be kept at 4ºC O/N.</p> |
''Taís'' | ''Taís'' | ||
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| + | ==''' ColiGuard'''== | ||
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| + | ====Starting the biobricks of kill mechanism with colicins==== | ||
| + | *<p style=”text-align:justify;”>We made the culture for miniprep of the transformed cells with the biobrick BBa_J131009 (operon colicin-E2). We want to isolate the CeaB (colicin protein) and the CeiB (immunity protein) in order to put them separately in the conjugative plasmid and in the genome of ''E. coli'' respectively and according to the killing mechanism (explained in the project description). To fulfill this aim, we designed 3 “primers” for isolating. For isolating the CeaB, we used like forward primer, '''VF2''' (general primer forward for backbone plasmid) and like reverse primer, our oligonucleotide designed for us, '''ColR'''. We also designed 2 primers forward and reverse for isolating the CeiB: '''Anticol F''' and '''Anticol R'''.</p> | ||
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| + | ''Ane e Luige'' | ||
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{{:Team:UNICAMP-Brazil/inc_rodape}} | {{:Team:UNICAMP-Brazil/inc_rodape}} | ||
Latest revision as of 02:22, 22 October 2009
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