Team:UNICAMP-Brazil/Notebooks/September 16

(Difference between revisions)

Latest revision as of 02:38, 22 October 2009

The digestion with the HindIII restriction enzyme was performed according to Protocol 14. In place of the second enzyme that is used in this reaction, it was used the same volume of autoclaved distilled water.

Gabriel

After the O/N period we transformed the ligations PY1 + BBa_J23100 and PY2 + BBa_J23100 into electrocompetent E. coli bacteria, strain DH10B. We followed Protocol 3 (Electroporation) without modifications.

After the transformation we plated the transformed cells in LB-AMP plates and let them grow at 37ºC for an O/N period.

Fabi and Léo

@@ Line 4: / Line 4: @@
 ==''' ColiGuard '''==
-====F plasmid's digestion with HindIII====
+====F plasmid's digestion with ''HindIII''====
+*<p style=”text-align:justify;”>The digestion with the ''Hind''III restriction enzyme was performed according to [https://2009.igem.org/Team:UNICAMP-Brazil/Protocols/Digestion_reaction Protocol 14]. In place of the second enzyme that is used in this reaction, it was used the same volume of autoclaved distilled water.</p>
-*<p style=”text-align:justify;”>From a permanent culture of bacteria containig the F plasmid, a sample was innoculated into liquid LB-AMP medium and let grown O/N at 37°C.</p>
 ''Gabriel''
+==== PY Promoter - Transformation ====
+*<p style=”text-align:justify;”>After the O/N period we transformed the ligations PY1 + BBa_J23100 and PY2 + BBa_J23100 into electrocompetent ''E. coli'' bacteria, strain DH10B. We followed [https://2009.igem.org/Team:UNICAMP-Brazil/Protocols/Electroporation Protocol 3] (Electroporation) without modifications.</p>
+*<p style=”text-align:justify;”>After the transformation we plated the transformed cells in LB-AMP plates and let them grow at 37ºC for an O/N period.</p>
+''Fabi and Léo''
 {{:Team:UNICAMP-Brazil/inc_rodape}}