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Team:UNICAMP-Brazil/Notebooks/September 16
From 2009.igem.org
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==''' ColiGuard '''== | ==''' ColiGuard '''== | ||
| - | ====F plasmid's digestion with HindIII==== | + | ====F plasmid's digestion with ''HindIII''==== |
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| + | *<p style=”text-align:justify;”>The digestion with the ''Hind''III restriction enzyme was performed according to [https://2009.igem.org/Team:UNICAMP-Brazil/Protocols/Digestion_reaction Protocol 14]. In place of the second enzyme that is used in this reaction, it was used the same volume of autoclaved distilled water.</p> | ||
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''Gabriel'' | ''Gabriel'' | ||
| + | ==== PY Promoter - Transformation ==== | ||
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| + | *<p style=”text-align:justify;”>After the O/N period we transformed the ligations PY1 + BBa_J23100 and PY2 + BBa_J23100 into electrocompetent ''E. coli'' bacteria, strain DH10B. We followed [https://2009.igem.org/Team:UNICAMP-Brazil/Protocols/Electroporation Protocol 3] (Electroporation) without modifications.</p> | ||
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| + | *<p style=”text-align:justify;”>After the transformation we plated the transformed cells in LB-AMP plates and let them grow at 37ºC for an O/N period.</p> | ||
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| + | ''Fabi and Léo'' | ||
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{{:Team:UNICAMP-Brazil/inc_rodape}} | {{:Team:UNICAMP-Brazil/inc_rodape}} | ||
Latest revision as of 02:38, 22 October 2009
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