Team:UNICAMP-Brazil/Notebooks/September 23

(Difference between revisions)

Latest revision as of 03:06, 22 October 2009

The ligation reaction was assembled according to Protocol 11. Both the plasmid samples were used in recircularization. The reaction tubes was kept O/N at 4°C.

Gabriel

Today we performed minipreps from yesterday's inoculated cultures, according to Protocol 2.

Marcelo

We got apparently good transformations but we have to prove the correct position of DNA inserted. So, we performed colony PCR with right primers.

We didn’t get the band size expected and we found unspecific amplification, therefore we will make miniprep and we will try PCR again.

Ane

@@ Line 8: / Line 8: @@
 ====F plasmid recircularization====
-*<p style=”text-align:justify;”>The ligation reaction was assembled according to [https://2009.igem.org/Team:UNICAMP-Brazil/Protocols/T4_DNA_Ligase Protocol 11]. Both the plasmid samples were used to recircularization. The reaction tubes was kept O/N at 4°C.</p>
+*<p style=”text-align:justify;”>The ligation reaction was assembled according to [https://2009.igem.org/Team:UNICAMP-Brazil/Protocols/T4_DNA_Ligase Protocol 11]. Both the plasmid samples were used in recircularization. The reaction tubes was kept O/N at 4°C.</p>
 ''Gabriel''
 ====finO and finP - Still Trying to Confirm our Biobricks====
@@ Line 27: / Line 26: @@
 [[Image:23_sept..jpg‎ ‎ |center|‎]]
-We didn’t get the band size expected and we found unspecific amplification, therefore we will make miniprep and we will try PCR again.
+*<p style=”text-align:justify;”>We didn’t get the band size expected and we found unspecific amplification, therefore we will make miniprep and we will try PCR again.</p>
 ''Ane''