Team:UNICAMP-Brazil/Notebooks/September 23

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====F plasmid recircularization====
====F plasmid recircularization====
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*<p style=”text-align:justify;”>The ligation reaction was assembled according to [https://2009.igem.org/Team:UNICAMP-Brazil/Protocols/CIAP_Dephosphorylation Protocol 10]. Both the plasmid samples were used to recircularization. The reaction tubes was kept O/N at 4°C.</p>
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*<p style=”text-align:justify;”>The ligation reaction was assembled according to [https://2009.igem.org/Team:UNICAMP-Brazil/Protocols/T4_DNA_Ligase Protocol 11]. Both the plasmid samples were used in recircularization. The reaction tubes was kept O/N at 4°C.</p>
''Gabriel''
''Gabriel''
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====finO and finP - Still Trying to Confirm our Biobricks====
====finO and finP - Still Trying to Confirm our Biobricks====
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*<p style=”text-align:justify;”>Today we performed minipreps from yesterday's inoculated cultures.</p>
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*<p style=”text-align:justify;”>Today we performed minipreps from yesterday's inoculated cultures, according to [https://2009.igem.org/Team:UNICAMP-Brazil/Protocols/Mini-Prep Protocol 2].</p>
''Marcelo''
''Marcelo''
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==== CeaB and CeiB: colony PCR ====
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*<p style=”text-align:justify;”>We got apparently good transformations but we have to prove the correct position of DNA inserted. So, we performed colony PCR with right primers.</p>
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[[Image:23_sept..jpg‎ ‎ |center|‎]]
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*<p style=”text-align:justify;”>We didn’t get the band size expected and we found unspecific amplification, therefore we will make miniprep and we will try PCR again.</p>
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''Ane''
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{{:Team:UNICAMP-Brazil/inc_rodape}}
{{:Team:UNICAMP-Brazil/inc_rodape}}

Latest revision as of 03:06, 22 October 2009

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ColiGuard

F plasmid recircularization

  • The ligation reaction was assembled according to Protocol 11. Both the plasmid samples were used in recircularization. The reaction tubes was kept O/N at 4°C.

Gabriel

finO and finP - Still Trying to Confirm our Biobricks

  • Today we performed minipreps from yesterday's inoculated cultures, according to Protocol 2.

Marcelo


CeaB and CeiB: colony PCR

  • We got apparently good transformations but we have to prove the correct position of DNA inserted. So, we performed colony PCR with right primers.

‎

  • We didn’t get the band size expected and we found unspecific amplification, therefore we will make miniprep and we will try PCR again.

Ane





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