Team:UNICAMP-Brazil/Notebooks/October 18

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==''' ColiGuard '''==
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==== PY Promoter - Colony-PCR ====
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*<p style=”text-align:justify;”>We got one single colony from the transformation we did yesterday!</p>
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*<p style=”text-align:justify;”>We performed a colony-pcr to confirm that this colony has our plasmid.</p>
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*<p style=”text-align:justify;”>We used 2 pairs of primers - VF/VR and PY primers - and analyzed the results of our PCR in an agarose gel:</p>
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[[Image:py_gel_9.png|200px|center]]
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*<p style=”text-align:justify;”>The expected size for PY1 + RFP + BBa_B0015 amplified with VF/VR primers is 1279 bp.</p>
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*<p style=”text-align:justify;”>The expected size for PY1 + RFP + BBa_B0015 amplified with PY primers is 133 bp.</p>
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*<p style=”text-align:justify;”>As we can observe in the gel photo, the colony presented bands compatible with the size expected for both pairs of primers!!!! =)</p>
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''Fabi and Léo''
==''' YeastGuard '''==
==''' YeastGuard '''==
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====Yeast experiments====
====Yeast experiments====
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*<p style=”text-align:justify;”>We did miniprep of 10 inocula of pDLD+YFP and 10 inocula of Adh1+YFP. We transformed the plasmids in competent yeast. Hope to find colonies tomorrow! =)</p>
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*<p style=”text-align:justify;”>We did miniprep of 10 inocula of pDLD+YFP and 10 inocula of ''ADH1''+YFP. We transformed the plasmids in competent yeast. Hope to find colonies tomorrow! =)</p>
''Raíssa and Taís''
''Raíssa and Taís''
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Latest revision as of 03:38, 22 October 2009

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ColiGuard

PY Promoter - Colony-PCR

  • We got one single colony from the transformation we did yesterday!

  • We performed a colony-pcr to confirm that this colony has our plasmid.

  • We used 2 pairs of primers - VF/VR and PY primers - and analyzed the results of our PCR in an agarose gel:

Py gel 9.png

  • The expected size for PY1 + RFP + BBa_B0015 amplified with VF/VR primers is 1279 bp.

  • The expected size for PY1 + RFP + BBa_B0015 amplified with PY primers is 133 bp.

  • As we can observe in the gel photo, the colony presented bands compatible with the size expected for both pairs of primers!!!! =)

Fabi and Léo

YeastGuard

Yeast experiments

  • We did miniprep of 10 inocula of pDLD+YFP and 10 inocula of ADH1+YFP. We transformed the plasmids in competent yeast. Hope to find colonies tomorrow! =)

Raíssa and Taís




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