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Team:UNICAMP-Brazil/Notebooks/October 18
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*<p style=”text-align:justify;”>We performed a colony-pcr to confirm that this colony has our plasmid.</p> | *<p style=”text-align:justify;”>We performed a colony-pcr to confirm that this colony has our plasmid.</p> | ||
| - | *<p style=”text-align:justify;”>We used 2 pairs of primers | + | *<p style=”text-align:justify;”>We used 2 pairs of primers - VF/VR and PY primers - and analyzed the results of our PCR in an agarose gel:</p> |
| + | [[Image:py_gel_9.png|200px|center]] | ||
| + | *<p style=”text-align:justify;”>The expected size for PY1 + RFP + BBa_B0015 amplified with VF/VR primers is 1279 bp.</p> | ||
| + | *<p style=”text-align:justify;”>The expected size for PY1 + RFP + BBa_B0015 amplified with PY primers is 133 bp.</p> | ||
| + | *<p style=”text-align:justify;”>As we can observe in the gel photo, the colony presented bands compatible with the size expected for both pairs of primers!!!! =)</p> | ||
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| + | ''Fabi and Léo'' | ||
==''' YeastGuard '''== | ==''' YeastGuard '''== | ||
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====Yeast experiments==== | ====Yeast experiments==== | ||
| - | *<p style=”text-align:justify;”>We did miniprep of 10 inocula of pDLD+YFP and 10 inocula of | + | *<p style=”text-align:justify;”>We did miniprep of 10 inocula of pDLD+YFP and 10 inocula of ''ADH1''+YFP. We transformed the plasmids in competent yeast. Hope to find colonies tomorrow! =)</p> |
''Raíssa and Taís'' | ''Raíssa and Taís'' | ||
{{:Team:UNICAMP-Brazil/inc_rodape}} | {{:Team:UNICAMP-Brazil/inc_rodape}} | ||
Latest revision as of 03:38, 22 October 2009
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