|
|
| Line 38: |
Line 38: |
| | |width=15%| Products in use | | |width=15%| Products in use |
| | |width=5%| Date | | |width=5%| Date |
| - | |- style="background: #cccccc;"
| |
| - | | style="background: #ccffcc;" |MP1
| |
| - | |<bbpart>pJ23100</bbpart> in <bbpart>J61002</bbpart>
| |
| - | |Strong constitutive promoter in J61002 (clones 1 + 2) <partinfo>pJ23100 SpecifiedComponents</partinfo>
| |
| - | |
| |
| - | | [[Paris/July 15| July 15]], [[Paris/August 17| Aug. 17]]
| |
| - | |- style="background: #cccccc;"
| |
| - | | style="background: #ccffcc;" |MP2
| |
| - | |<bbpart>pJ23107</bbpart> in <bbpart>J61002</bbpart>
| |
| - | |Medium constitutive promoter in J61002 (clones 1 + 2) <partinfo>pJ23107 SpecifiedComponents</partinfo>
| |
| - | |MP2.2
| |
| - | | [[Paris/July 15| July 15]], [[Paris/August 17| Aug. 17]]
| |
| - | |- style="background: #cccccc;"
| |
| - | | style="background: #ccffcc;" |MP3
| |
| - | |<bbpart>B0015</bbpart> in <bbpart>pSB1AK3</bbpart>
| |
| - | |Double terminator (clones 1 + 2) <partinfo>B0015 SpecifiedComponents</partinfo>
| |
| - | |MP3.3, MP3.4
| |
| - | | [[Paris/August 14| August 14]]
| |
| - | |- style="background: #cccccc;"
| |
| - | | style="background: #ccffcc;" |MP4
| |
| - | |<bbpart>I0500</bbpart> in <bbpart>pSB2K3</bbpart>
| |
| - | |AraC pBAD (clones 1 + 2) <partinfo>I0500 SpecifiedComponents</partinfo>
| |
| - | |
| |
| - | | [[Paris/July 16| July 16]]
| |
| - | |- style="background: #cccccc;"
| |
| - | | style="background: #ccffcc;" |MP5
| |
| - | |<bbpart>BBa_B0030</bbpart> in <bbpart>pSB1A2</bbpart>
| |
| - | |Strong RBS (clones 1 + 2) <partinfo>B0030 SpecifiedComponents</partinfo>
| |
| - | |
| |
| - | | [[Paris/July 18| July 18]]
| |
| - | |- style="background: #cccccc;"
| |
| - | | style="background: #ccffcc;" |MP6
| |
| - | |<bbpart>BBa_E0422</bbpart> in <bbpart>pSB1A2</bbpart>
| |
| - | |ECFP (RBS+LVA+Term) (clones 1 + 2) <partinfo>E0422 SpecifiedComponents</partinfo>
| |
| - | |MP6'.1, MP6'.2
| |
| - | | [[Paris/July 18| July 18]], [[Paris/July 24| July 24]]
| |
| - | |- style="background: #cccccc;"
| |
| - | | style="background: #ccffcc;" |MP7
| |
| - | |<bbpart>BBa_E0241</bbpart> in <bbpart>pSB1A2</bbpart>
| |
| - | |PoPs to GFP converter (clones 1 + 2) <partinfo>BBa_E0241 DeepComponents</partinfo>
| |
| - | |MP7'.1
| |
| - | | [[Paris/July 18| July 18]], [[Paris/July 24| July 24]]
| |
| - | |- style="background: #cccccc;"
| |
| - | | style="background: #ccffcc;" |MP8
| |
| - | |<bbpart>BBa_E0840</bbpart> in <bbpart>pSB1A2</bbpart>
| |
| - | |gfp tri-part; strong rbs (clones 1 + 2) <partinfo>E0840 SpecifiedComponents</partinfo>
| |
| - | |
| |
| - | | [[Paris/July 18| July 18]]
| |
| - | |- style="background: #cccccc;"
| |
| - | | style="background: #ccffcc;" |MP9
| |
| - | |<bbpart>BBa_J61047</bbpart> in <bbpart>pSB1A2</bbpart>
| |
| - | |Cre ORF (clones 1 + 2) <partinfo>J61047 SpecifiedComponents</partinfo>
| |
| - | |MP9'.1, MP9'.2
| |
| - | | [[Paris/July 18| July 18]], [[Paris/July 24| July 24]]
| |
| - | |- style="background: #cccccc;"
| |
| - | | style="background: #ccffcc;" |MP10
| |
| - | |<bbpart>BBa_I0500</bbpart> in <bbpart>pSB2K3</bbpart>
| |
| - | |AraC pBAD (clones 1 + 2) <partinfo>I0500 SpecifiedComponents</partinfo>
| |
| - | |
| |
| - | | [[Paris/July 20| July 20]]
| |
| - | |- style="background: #cccccc;"
| |
| - | | style="background: #ccffcc;" |MP11
| |
| - | |<bbpart>BBa_pJ23107</bbpart> in <bbpart>p61002</bbpart>
| |
| - | |Medium constitutive promoter in J61002 (clones 3 + 4) <partinfo>pJ23107 SpecifiedComponents</partinfo>
| |
| - | |MP11'.3
| |
| - | | [[Paris/July 20| July 20]], [[Paris/July 23| July 23]]
| |
| - | |- style="background: #cccccc;"
| |
| - | | style="background: #ccffcc;" |MP12
| |
| - | |<bbpart>BBa_P1003</bbpart> in <bbpart>pSB1A2</bbpart>
| |
| - | |KanR <partinfo>P1003 SpecifiedComponents</partinfo>
| |
| - | |
| |
| - | | [[Paris/July 27| July 27]]
| |
| - | |- style="background: #cccccc;"
| |
| - | | style="background: #ccffcc;" |MP13
| |
| - | |<bbpart>BBa_J61002</bbpart>
| |
| - | |ptet-mRFP <partinfo>J61002 SpecifiedComponents</partinfo>
| |
| - | |MP13.1, MP13.2
| |
| - | | [[Paris/August 14| August 14]]
| |
| - | |- style="background: #cccccc;"
| |
| - | | style="background: #ccffcc;" |MP14
| |
| - | |<bbpart>BBa_J61025</bbpart>
| |
| - | |Medium constitutive promoter in J61002 (clones 1 + 2) <partinfo>BBa_J61025 SpecifiedComponents</partinfo>
| |
| - | |MP14.1, MP14.2
| |
| - | | [[Paris/August 14| August 14]]
| |
| - | |- style="background: #cccccc;"
| |
| - | | style="background: #ccffcc;" |L1
| |
| - | |<bbpart>BBa_I0500</bbpart> in <bbpart>J61002</bbpart>
| |
| - | |construct with pBad promotor regulating expression of mRFP <partinfo>I0500 SpecifiedComponents</partinfo>
| |
| - | |L1".1, L1".2
| |
| - | | [[Paris/August 14| August 14]]
| |
| - | |- style="background: #cccccc;"
| |
| - | | style="background: #ccffcc;" |
| |
| - | |AttB
| |
| - | |
| |
| - | |MP15
| |
| - | | [[Paris/August 14| August 14]]
| |
| - | |- style="background: #cccccc;"
| |
| - | | style="background: #ccffcc;" |
| |
| - | |AttP
| |
| - | |Finished
| |
| - | |MP16
| |
| - | | [[Paris/July 25| July 25]], [[Paris/July 27| July 27]], [[Paris/August 7| Aug 7]]
| |
| - | |- style="background: #cccccc;"
| |
| - | | style="background: #ccffcc;" |L2
| |
| - | |Lox66DapAE.coli in <bbpart>pSB1A2</bbpart>
| |
| - | |Insertion of lox66DapAE.coli construct as a biobrick in pSB1A2 plasmid
| |
| - | http://parts.mit.edu/registry/images/9/9f/Part_icon_dna.png
| |
| - | http://parts.mit.edu/registry/images/7/7b/Part_icon_cds.png
| |
| - | |
| |
| - | | [[Paris/July 25| July 25]]
| |
| - | |- style="background: #cccccc;"
| |
| - | | style="background: #ccffcc;" |L6
| |
| - | |<bbpart>BBa_B0030</bbpart>+<bbpart>BBa_J61047</bbpart>
| |
| - | |strong RBS(B0030)-Cre ORF
| |
| - | http://parts.mit.edu/r/parts/images/partbypart/icon_rbs.png
| |
| - | http://parts.mit.edu/registry/images/7/7b/Part_icon_cds.png
| |
| - | |L6.1, L6.2, L6.3, L6".1, L6".2
| |
| - | | [[Paris/July 25| July 25]], [[Paris/July 27| July 27]]
| |
| - | |- style="background: #cccccc;"
| |
| - | | style="background: #ccffcc;" |L9''
| |
| - | |lox66 in <bbpart>pSB1A2</bbpart>
| |
| - | |
| |
| - | |
| |
| - | |[[Paris/July 25| July 25]], [[Paris/July 30| July 30]]
| |
| - | |- style="background: #cccccc;"
| |
| - | | style="background: #ccffcc;" |L10''
| |
| - | |lox71 in <bbpart>pSB1A2</bbpart>
| |
| - | |
| |
| - | |
| |
| - | |[[Paris/July 25| July 25]], [[Paris/July 30| July 30]]
| |
| - | |- style="background: #cccccc;"
| |
| - | | style="background: #ccffcc;" |L12
| |
| - | |lox71-gfp in pSB1A2
| |
| - | |construction of the biobrick lox71-gfp
| |
| - | http://parts.mit.edu/registry/images/9/9f/Part_icon_dna.png
| |
| - | http://parts.mit.edu/r/parts/images/partbypart/icon_reporter.png
| |
| - | |L12.1 & L12.2
| |
| - | |[[Paris/July 30| July 30]]
| |
| - | |- style="background: #cccccc;"
| |
| - | | style="background: #ccffcc;" |L26
| |
| - | |araC/pbad>>RBS-Cre in <bbpart>J61002</bbpart>
| |
| - | |ligation ([[Paris/August 13| Aug.13]]) of RBS-Cre as a CDS behind pbad in <bbpart>J61002</bbpart>
| |
| - | |L26.1 (no more) & L26.2
| |
| - | |[[Paris/August 17| Aug. 17]]
| |
| - | |- style="background: #cccccc;"
| |
| - | | style="background: #ccffcc;" |L29
| |
| - | |gfptripart-lox66 <bbpart>pSB1A2</bbpart>
| |
| - | |ligation ([[Paris/August 13| Aug.13]]) of gfp-tripart in front of lox66
| |
| - | |L29.1 & L29.2
| |
| - | |[[Paris/August 17| Aug. 17]]
| |
| - | |- style="background: #cccccc;"
| |
| - | | style="background: #ccffcc;" |L30
| |
| - | |dapAp>>mRFP in <bbpart>J61002</bbpart>
| |
| - | |dapAp promotor placed([[Paris/August 13| Aug.13]]) upstream of mRFP <bbpart>J61002</bbpart>
| |
| - | |L30A1 & L30A2
| |
| - | |[[Paris/August 17| Aug. 17]]
| |
| - | |- style="background: #cccccc;"
| |
| - | | style="background: #ccffcc;" |L31
| |
| - | |lox71-<bbpart>B0015</bbpart>(T) in <bbpart>pSB1A2</bbpart>
| |
| - | |
| |
| - | |L31.1 & L31.3
| |
| - | |[[Paris/August 20| Aug. 20]]
| |
| - | |- style="background: #cccccc;"
| |
| - | | style="background: #ccffcc;" |L32
| |
| - | |<bbpart>B0015</bbpart>(T)-lox66 in <bbpart>pSB1A2</bbpart>
| |
| - | |
| |
| - | |L32.1 & L32.2
| |
| - | |[[Paris/August 20| Aug. 20]]
| |
| - | |- style="background: #cccccc;"
| |
| - | | style="background: #ccffcc;" |L34
| |
| - | |pTet>>lox71-gfptripart in <bbpart>pSB1A2</bbpart>
| |
| - | |
| |
| - | |L34.1 & L34.2
| |
| - | |[[Paris/August 20| Aug. 20]]
| |
| - | |- style="background: #cccccc;"
| |
| - | | style="background: #ccffcc;" |L35
| |
| - | |araC/pBad>>lox71-gfptripart in <bbpart>pSB1A2</bbpart>
| |
| - | |il manque la seq. de 740-1410
| |
| - | |L35.1 & L35.2
| |
| - | |[[Paris/August 20| Aug. 20]]
| |
| - | |- style="background: #cccccc;"
| |
| - | | style="background: #ccffcc;" |L36
| |
| - | |lox66-mRFP
| |
| - | |
| |
| - | |L36.1 & L36.2
| |
| - | |[[Paris/August 20| Aug. 20]]
| |
| - | |- style="background: #cccccc;"
| |
| - | | style="background: #ccffcc;" |L37
| |
| - | |lox66-RBS-dapAcoli
| |
| - | |
| |
| - | |L37.1 & L37.2
| |
| - | |[[Paris/August 20| Aug. 20]]
| |
| - | |- style="background: #cccccc;"
| |
| - | | style="background: #ccffcc;" |L39
| |
| - | |RBS-dapAcoli cloned in <bbpart>pSB1A2</bbpart>
| |
| - | |
| |
| - | |L39.1 & L39.2
| |
| - | |[[Paris/August 20| Aug. 20]]
| |
| - | |- style="background: #cccccc;"
| |
| - | | style="background: #ccffcc;" |L46
| |
| - | |pTet>>lox71-B0015(T) in <bbpart>pSB1A2</bbpart>
| |
| - | |
| |
| - | |L46.1
| |
| - | |[[Paris/September 18| Sep. 18]]
| |
| - | |- style="background: #cccccc;"
| |
| - | | style="background: #ccffcc;" |L53
| |
| - | |B0015(T)-lox66-RBSDapAcoli in <bbpart>pSB1A2</bbpart>
| |
| - | |
| |
| - | |L53.7
| |
| - | |[[Paris/September 18| Sep. 18]]
| |
| - | |- style="background: #cccccc;"
| |
| - | | style="background: #ccffcc;" |L56
| |
| - | |B0015(T)-lox66-RBSdapAsubtilis in <bbpart>pSB1A2</bbpart>
| |
| - | |
| |
| - | |L56.3 (attention, seq a partiellement foiré, refaire seq)
| |
| - | |[[Paris/September 18| Sep. 18]]
| |
| - | |- style="background: #cccccc;"
| |
| - | | style="background: #ccffcc;" |L59
| |
| - | |pTet>>lox71-gfptripart-lox66-mRFP <bbpart>pSB1A2</bbpart>
| |
| - | |
| |
| - | |L59.6 (attention, seq avec O40 a foiré, mais les extrémités sont bonnes, je pense que c ok car les colonies sont vertes)
| |
| - | |[[Paris/September 18| Sep. 18]]
| |
| - | |- style="background: #cccccc;"
| |
| - | | style="background: #ccffcc;" |L64
| |
| - | |RBSdapAsubtilis cloned in <bbpart>pSB1A2</bbpart>
| |
| - | |
| |
| - | |L564.1 & L64.2
| |
| - | |[[Paris/September 18| Sep. 18]]
| |
| - | |- style="background: #cccccc;"
| |
| - | | style="background: #ccffcc;" |MPT1
| |
| - | |Lox66-DapAColi
| |
| - | |pTOPO P5
| |
| - | |MPT1.1, MPT1.2
| |
| - | |[[Paris/August 7| Aug 7]]
| |
| - | |- style="background: #cccccc;"
| |
| - | | style="background: #ccffcc;" |MPT3
| |
| - | |Lox66-DapASubtilis
| |
| - | |pTOPO P6
| |
| - | |MPT3.2, MPT3.1
| |
| - | |[[Paris/August 7| Aug 7]]
| |
| - | |- style="background: #cccccc;"
| |
| - | | style="background: #ccffcc;" |MPT4
| |
| - | |RBS-DapAColi
| |
| - | |pTOPO P8
| |
| - | |MPT4.1, .2, .3, .4& .5
| |
| - | |[[Paris/August 7| Aug 7]]
| |
| - | |- style="background: #cccccc;"
| |
| - | | style="background: #ccffcc;" |MPT5
| |
| - | |RBS-DapASubtilis
| |
| - | |pTOPO P9
| |
| - | |MPT5.1, .2 &.3
| |
| - | |[[Paris/August 7| Aug 7]]
| |
| - | |- style="background: #cccccc;"
| |
| - | | style="background: #ccffcc;" |MP15
| |
| - | |pKD46
| |
| - | |
| |
| - | |
| |
| - | |[[Paris/October 2| Oct 2]]
| |
| | |} | | |} |
| | | | |
| Line 316: |
Line 58: |
| | |width=5%| []ng/µL | | |width=5%| []ng/µL |
| | |width=5%| Date | | |width=5%| Date |
| - | |- style="background: #cccccc;"
| |
| - | | style="background: #FF99FF;" |D1
| |
| - | |<bbpart>pJ23100</bbpart>
| |
| - | |<bbpart>pJ23100</bbpart>
| |
| - | |Spe1
| |
| - | |Pst1
| |
| - | |2096
| |
| - | |Vector with the J23100 promoter ready for insertion of a RBS/CDS insert
| |
| - | |Ø (24/09/07)
| |
| - | |34
| |
| - | |[[Paris/July 17| July 17]]
| |
| - | |- style="background: #cccccc;"
| |
| - | | style="background: #ccffcc;" |D2
| |
| - | |<bbpart>mRFP dblTer</bbpart>
| |
| - | |<bbpart>pJ23100</bbpart>
| |
| - | |Spe1
| |
| - | |Pst1
| |
| - | |887
| |
| - | |
| |
| - | |
| |
| - | |27
| |
| - | |[[Paris/July 17| July 17]]
| |
| - | |- style="background: #cccccc;"
| |
| - | | style="background: #FF99FF;" |D3
| |
| - | |<bbpart>pJ23107</bbpart>
| |
| - | |<bbpart>pJ23107</bbpart>
| |
| - | |Spe1
| |
| - | |Pst1
| |
| - | |2096
| |
| - | |Vector with the J23107 promoter ready for insertion of a RBS/CDS insert
| |
| - | |Ø (24/09/07)
| |
| - | |32
| |
| - | |[[Paris/July 17| July 17]]
| |
| - | |- style="background: #cccccc;"
| |
| - | | style="background: #FF99FF;" |D4
| |
| - | |<bbpart>mRFP dblTer</bbpart>
| |
| - | |<bbpart>pJ23107</bbpart>
| |
| - | |Spe1
| |
| - | |Pst1
| |
| - | |887
| |
| - | |
| |
| - | |
| |
| - | |25
| |
| - | |[[Paris/July 17| July 17]]
| |
| - | |- style="background: #cccccc;"
| |
| - | | style="background: #FF99FF;" |D5
| |
| - | |<bbpart>pJ23100</bbpart>
| |
| - | |<bbpart>pJ23100</bbpart>
| |
| - | |EcoR1
| |
| - | |Spe1
| |
| - | |2925
| |
| - | |The vector with the mRFP gene ready for insertion of a promoter. We are not sure the digestion worked properly.
| |
| - | |
| |
| - | |28&29
| |
| - | |[[Paris/July 17| July 17]]
| |
| - | |- style="background: #cccccc;"
| |
| - | | style="background: #FF99FF;" |D6
| |
| - | |<bbpart>pJ23107</bbpart>
| |
| - | |<bbpart>pJ23107</bbpart>
| |
| - | |EcoR1
| |
| - | |Spe1
| |
| - | |2925
| |
| - | |The vector with the mRFP gene ready for insertion of a promoter. We are not sure the digestion worked properly.
| |
| - | |
| |
| - | |31&27
| |
| - | |[[Paris/July 17| July 17]]
| |
| - | |- style="background: #cccccc;"
| |
| - | | style="background: #ccffcc;" |D7
| |
| - | |Lox66 DapA E.coli
| |
| - | |Lox66 DapA E.coli (PCR product)
| |
| - | |EcoR1
| |
| - | |Pst1
| |
| - | |977
| |
| - | |
| |
| - | |
| |
| - | |24
| |
| - | |[[Paris/July 17| July 17]]
| |
| - | |- style="background: #cccccc;"
| |
| - | | style="background: #ccffcc;" |D8
| |
| - | |Lox66 DapA E.coli
| |
| - | |Lox66 DapA E.coli (PCR product)
| |
| - | |Xba1
| |
| - | |Pst1
| |
| - | |962
| |
| - | |
| |
| - | |
| |
| - | |44
| |
| - | |[[Paris/July 17| July 17]]
| |
| - | |- style="background: #cccccc;"
| |
| - | | style="background: #FF99FF;" |D9
| |
| - | |<bbpart>pJ23100</bbpart> ES vector
| |
| - | |<bbpart>pJ23100</bbpart> (MP1.1 & MP1.2)
| |
| - | |EcoI
| |
| - | |SpeI
| |
| - | |2925
| |
| - | |Vector with mRFP RBS/CDS ready for insertion promoter (fw-insert)
| |
| - | |
| |
| - | |80&43
| |
| - | |[[Paris/July 21| July 21]]
| |
| - | |- style="background: #cccccc;"
| |
| - | | style="background: #ccffcc;" |D10
| |
| - | |<bbpart>pJ23100</bbpart> promoter fw-insert
| |
| - | |<bbpart>pJ23100</bbpart> (MP1.1 & MP1.2)
| |
| - | |EcoI
| |
| - | |SpeI
| |
| - | |58
| |
| - | |J23100 promoter ready for use as a fw-insert
| |
| - | |
| |
| - | |35&28
| |
| - | |[[Paris/July 21| July 21]]
| |
| - | |- style="background: #cccccc;"
| |
| - | | style="background: #FF99FF;" |D11
| |
| - | |<bbpart>pJ23107</bbpart> ES vector
| |
| - | |<bbpart>pJ23107</bbpart> (MP2.1, MP2.2, MP2.3 & MP2.4)
| |
| - | |EcoRI
| |
| - | |SpeI
| |
| - | |2925
| |
| - | |Vector with mRFP RBS/CDS ready for insertion of a fw-insert promoter
| |
| - | |
| |
| - | |49,35,28&41
| |
| - | |[[Paris/July 21| July 21]]
| |
| - | |- style="background: #cccccc;"
| |
| - | | style="background: #ccffcc;" |D12
| |
| - | |<bbpart>pJ23107</bbpart> promoter fw-insert
| |
| - | |<bbpart>pJ23107</bbpart> (MP2.1, MP2.2, MP2.3 & MP2.4)
| |
| - | |EcoRI
| |
| - | |SpeI
| |
| - | |58
| |
| - | |J23107 promoter ready for usage as a fw-insert
| |
| - | |
| |
| - | |23,25,32&32
| |
| - | |[[Paris/July 21| July 21]]
| |
| - | |- style="background: #cccccc;"
| |
| - | | style="background: #ccffcc;" |D13
| |
| - | |<bbpart>mRFP</bbpart>
| |
| - | |<bbpart>pJ23107</bbpart> (MP2.2, MP2.3 & MP2.4)
| |
| - | |SpeI
| |
| - | |PstI
| |
| - | |887
| |
| - | |mRFP RBS/CDS/T sequence, can it be used as a backward insert? => yes but you will not be able to add any biobrick after it
| |
| - | |
| |
| - | |21&41
| |
| - | |[[Paris/July 21| July 21]]
| |
| - | |- style="background: #cccccc;"
| |
| - | | style="background: #ccffcc;" |D14
| |
| - | |Cre ORF
| |
| - | |<bbpart>J61047</bbpart> MP9.1 & MP9.2
| |
| - | |XbaI
| |
| - | |PstI
| |
| - | |1063
| |
| - | |Cre ORF extracted for usage as a bw-insert
| |
| - | |Ø D14.1
| |
| - | |38&41
| |
| - | |[[Paris/July 21| July 21]]
| |
| - | |- style="background: #cccccc;"
| |
| - | | style="background: #FF99FF;" |D15
| |
| - | |<bbpart>B0030</bbpart> bw-vector
| |
| - | |<bbpart>B0030</bbpart> MP5.1 & MP5.2
| |
| - | |SpeI
| |
| - | |PstI
| |
| - | |2076
| |
| - | |bacward vector for cloning a CDS bw-insert after the B0030 RBS
| |
| - | |
| |
| - | |87&42
| |
| - | |[[Paris/July 21| July 21]]
| |
| - | |- style="background: #cccccc;"
| |
| - | | style="background: #ccffcc;" |D18
| |
| - | |BB dig. lox66DapAE.coli
| |
| - | |lox66-DapAE.coli P5 PCR product
| |
| - | |EcoRI
| |
| - | |PstI
| |
| - | |~960
| |
| - | |PCR product digested for cloning as a BioBrick
| |
| - | |Ø
| |
| - | |44
| |
| - | |[[Paris/July 21| July 21]]
| |
| - | |- style="background: #cccccc;"
| |
| - | | style="background: #ccffcc;" |D19
| |
| - | |BB dig. lox66DapAsubtilis
| |
| - | |lox66-DapAsubtilis P6 PCR product
| |
| - | |EcoRI
| |
| - | |PstI
| |
| - | |~960
| |
| - | |PCR product digested for cloning as a BioBrick
| |
| - | |Ø
| |
| - | |
| |
| - | |[[Paris/July 21| July 21]]
| |
| - | |- style="background: #cccccc;"
| |
| - | | style="background: #ccffcc;" |D19'
| |
| - | |BB dig. lox66DapAsubtilis
| |
| - | |lox66-DapAsubtilis P6' PCR product
| |
| - | |EcoRI
| |
| - | |PstI
| |
| - | |~960
| |
| - | |PCR product digested for cloning as a BioBrick
| |
| - | |
| |
| - | |16
| |
| - | |[[Paris/July 30| July 30]]
| |
| - | |- style="background: #cccccc;"
| |
| - | | style="background: #ccffcc;" |D20
| |
| - | |bw-insert lox66-DapAE.coli
| |
| - | |lox66-DapAE.coli P5 PCR product
| |
| - | |XbaI
| |
| - | |PstI
| |
| - | |~960
| |
| - | |PCR product digested for usage as a backward insert
| |
| - | |Ø
| |
| - | |
| |
| - | |[[Paris/July 21| July 21]]
| |
| - | |- style="background: #cccccc;"
| |
| - | | style="background: #ccffcc;" |D21
| |
| - | |bw-insert lox66-DapAsubtilis
| |
| - | |lox66-DapAsubtilis P6 PCR product
| |
| - | |XbaI
| |
| - | |PstI
| |
| - | |~960
| |
| - | |PCR product digested for usage as a backward insert
| |
| - | |Ø
| |
| - | |
| |
| - | |[[Paris/July 21| July 21]]
| |
| - | |- style="background: #cccccc;"
| |
| - | | style="background: #ccffcc;" |D21'
| |
| - | |bw-insert lox66-DapAsubtilis
| |
| - | |lox66-DapAsubtilis P6' PCR product
| |
| - | |XbaI
| |
| - | |PstI
| |
| - | |~960
| |
| - | |PCR product digested for cloning as a BI
| |
| - | |
| |
| - | |34
| |
| - | |[[Paris/July 30| July 30]]
| |
| - | |- style="background: #cccccc;"
| |
| - | | style="background: #FF99FF;" |D22
| |
| - | |pSB1A2 open vector
| |
| - | |<bbpart>BBa_J61047</bbpart> (MP9.1 & MP9.2)
| |
| - | |EcoI
| |
| - | |PstI
| |
| - | |Ø D22.1
| |
| - | |Open pSB1A2 vector for BioBrick EcoRI/PstI insertio
| |
| - | |Ø (24/09/07)
| |
| - | |D22.2:59
| |
| - | |[[Paris/July 23| July 23]],[[Paris/August 13 | Aug. 13 ]]
| |
| - | |- style="background: #cccccc;"
| |
| - | | style="background: #ccffcc;" |D23
| |
| - | |AraC/pBad promoter fw-insert
| |
| - | |<bbpart>BBa_I0500</bbpart> (MP4.1 & MP4.2)
| |
| - | |EcoI
| |
| - | |SpeI
| |
| - | |1233
| |
| - | |AraC/pBad promoter ready for use as a forward insert
| |
| - | |Ø D23.1
| |
| - | |93&53
| |
| - | |[[Paris/July 23| July 23]]
| |
| - | |- style="background: #cccccc;"
| |
| - | | style="background: #ccffcc;" |D24
| |
| - | |eCFP bw-insert
| |
| - | |<bbpart>BBa_E0422</bbpart> (MP6.1 & MP6.2)
| |
| - | |XbaI
| |
| - | |PstI
| |
| - | |
| |
| - | |eCFP (RBS-OFR_LVA-T) ready for use as a backward insert
| |
| - | |
| |
| - | |56&55
| |
| - | |[[Paris/July 23| July 23]]
| |
| - | |- style="background: #cccccc;"
| |
| - | | style="background: #ccffcc;" |D26
| |
| - | |gfp-tripart
| |
| - | |<bbpart>BBa_E0840</bbpart> (MP8.1 & MP8.2)
| |
| - | |XbaI
| |
| - | |PstI
| |
| - | |
| |
| - | |gfp-tri part (strongRBS-ORF-T) ready for use as a backward insert
| |
| - | |
| |
| - | |47&47
| |
| - | |[[Paris/July 23| July 23]]
| |
| - | |- style="background: #cccccc;"
| |
| - | | style="background: #ccffcc;" |D27
| |
| - | |lox71-ftsZ bw-insert
| |
| - | |lox71-ftsZ PCR product P7
| |
| - | |XbaI
| |
| - | |PstI
| |
| - | |
| |
| - | |lox71-ftsZ(unmutated) ready for use as a backward insert
| |
| - | |Ø
| |
| - | |
| |
| - | |[[Paris/July 23| July 23]]
| |
| - | |- style="background: #cccccc;"
| |
| - | | style="background: #ccffcc;" |D27'
| |
| - | |lox71-ftsZ bw-insert
| |
| - | |lox71-ftsZ PCR product P7
| |
| - | |XbaI
| |
| - | |PstI
| |
| - | |
| |
| - | |lox71-ftsZ(unmutated) ready for use as a backward insert
| |
| - | |
| |
| - | |24
| |
| - | |[[Paris/July 30| July 30]]
| |
| - | |- style="background: #cccccc;"
| |
| - | | style="background: #ccffcc;" |D28
| |
| - | |RBS-DapAColi BB
| |
| - | |P8 (RBS-DapAColi)
| |
| - | |EcoRI
| |
| - | |PstI
| |
| - | |~950
| |
| - | |Biobrick
| |
| - | |
| |
| - | |54
| |
| - | |[[Paris/July 25| July 25]]
| |
| - | |- style="background: #cccccc;"
| |
| - | | style="background: #ccffcc;" |D29
| |
| - | |RBS-DapAColi
| |
| - | |P8 (RBS-DapAColi)
| |
| - | |XbaI
| |
| - | |PstI
| |
| - | |~950
| |
| - | |Backward insert
| |
| - | |
| |
| - | |44
| |
| - | |[[Paris/July 25| July 25]]
| |
| - | |- style="background: #cccccc;"
| |
| - | | style="background: #ccffcc;" |D30
| |
| - | |RBS-DapASubtilis BB
| |
| - | |P9 (RBS-DapASubtilis)
| |
| - | |EcoRI
| |
| - | |PstI
| |
| - | |~950
| |
| - | |Biobrick
| |
| - | |
| |
| - | |72
| |
| - | |[[Paris/July 25| July 25]]
| |
| - | |- style="background: #cccccc;"
| |
| - | | style="background: #ccffcc;" |D31
| |
| - | |RBS-DapASubtilis
| |
| - | |P9 (RBS-DapASubtilis)
| |
| - | |XbaI
| |
| - | |PstI
| |
| - | |~950
| |
| - | |Backward insert
| |
| - | |
| |
| - | |51
| |
| - | |[[Paris/July 25| July 25]]
| |
| - | |- style="background: #cccccc;"
| |
| - | | style="background: #ccffcc;" |D32
| |
| - | |Lox71-FtsA-FtsZ
| |
| - | |P10 Lox71-FtsA-FtsZ
| |
| - | |XbaI
| |
| - | |PstI
| |
| - | |~2600
| |
| - | |Backward insert - EcoRI site still presents : we can't use EcoRI
| |
| - | |
| |
| - | |47
| |
| - | |[[Paris/July 25| July 25]]
| |
| - | |- style="background: #cccccc;"
| |
| - | | style="background: #ccffcc;" |D33
| |
| - | |RBS(B0030)CreORF
| |
| - | |L6' (clone 1 & 2)
| |
| - | |XbaI
| |
| - | |PstI
| |
| - | |
| |
| - | |RBS-Cre digestion for use as a BI
| |
| - | |
| |
| - | |29&27
| |
| - | |[[Paris/July 30| July 30]],[[Paris/August 13| August 13]]
| |
| - | |- style="background: #cccccc;"
| |
| - | | style="background: #ccffcc;" |D34
| |
| - | |lox66
| |
| - | |L9' lox66
| |
| - | |SpeI
| |
| - | |PstI
| |
| - | |
| |
| - | |Biobrick plasmid carrying lox66 digested as a BV
| |
| - | |
| |
| - | |18
| |
| - | |[[Paris/July 30| July 30]]
| |
| - | |- style="background: #cccccc;"
| |
| - | | style="background: #ccffcc;" |D35
| |
| - | |lox71
| |
| - | |L10' lox71
| |
| - | |SpeI
| |
| - | |PstI
| |
| - | |
| |
| - | |Biobrick plasmid carrying lox71 digested as a BV
| |
| - | |
| |
| - | |
| |
| - | |[[Paris/July 30| July 30]],[[Paris/August 13| August 13]]
| |
| - | |- style="background: #cccccc;"
| |
| - | | style="background: #ccffcc;" |D36
| |
| - | |lox71-KmR-lox66
| |
| - | |P11 lox71-KmR-lox66
| |
| - | |EcoRI
| |
| - | |PstI
| |
| - | |
| |
| - | |lox71-KmR-lox66 digested as a biobrick insert
| |
| - | |
| |
| - | |
| |
| - | |[[Paris/July 30| July 30]]
| |
| - | |- style="background: #cccccc;"
| |
| - | | style="background: #ccffcc;" |D37
| |
| - | |lox71-gfp(tripart) BI
| |
| - | |L12.1 & L12.2 miniprep products (from [[Paris/July 30| 30/07]] ligation reaction)
| |
| - | |XbaI
| |
| - | |PstI
| |
| - | |
| |
| - | |lox71_RBS-gfp-double terminator extracted as a backward insert
| |
| - | |
| |
| - | |
| |
| - | |[[Paris/August 6 | Aug. 6 ]]
| |
| - | |- style="background: #cccccc;"
| |
| - | | style="background: #ccffcc;" |D38
| |
| - | |BB-insert lox66-DapAE.coli
| |
| - | |lox66-DapAE.coli in pTOPO (P5 PCR product)(clones 1+2)
| |
| - | |EcoRI
| |
| - | |PstI
| |
| - | |
| |
| - | |lox66-dapAE.coli extracted from pTOPO plasmid as a BB insert
| |
| - | |
| |
| - | |
| |
| - | |[[Paris/August 6| Aug. 6]]
| |
| - | |- style="background: #cccccc;"
| |
| - | | style="background: #ccffcc;" |D39'
| |
| - | |BI-insert lox66-DapAE.coli
| |
| - | |lox66-DapAE.coli in pTOPO (P5 PCR product)(clones 1+2)
| |
| - | |XbaI
| |
| - | |PstI
| |
| - | |
| |
| - | |lox66-dapAcoli extracted for usage as a backward insert
| |
| - | |
| |
| - | |
| |
| - | |[[Paris/August 7 | Aug. 7 ]]
| |
| - | |- style="background: #cccccc;"
| |
| - | | style="background: #ccffcc;" |D40
| |
| - | |BB-insert lox71-KmR-lox66
| |
| - | |L25.1 miniprep product (from ligation rxn of [[Paris/July 30| 30/07]]
| |
| - | |EcoRI
| |
| - | |PstI
| |
| - | |
| |
| - | |lox71-KmR-lox66 construct extracted for clonong as a biobrick
| |
| - | |
| |
| - | |
| |
| - | |[[Paris/August 6 | Aug. 6 ]]
| |
| - | |- style="background: #cccccc;"
| |
| - | | style="background: #FF99FF;" |D41
| |
| - | |araC/pBad-J61002 plasmid SP dig.
| |
| - | |L1.2, L1".1 & L1".2 miniprep product
| |
| - | |SpeI
| |
| - | |PstI
| |
| - | |
| |
| - | |J61002 plasmid containing araC/pBad promotor digested for insertion of a BI CDS
| |
| - | |
| |
| - | |
| |
| - | |[[Paris/August 13 | Aug. 13 ]]
| |
| - | |- style="background: #cccccc;"
| |
| - | | style="background: #FF99FF;" |D42
| |
| - | |Lox66 FV
| |
| - | |L9
| |
| - | |EcoRI
| |
| - | |XbaI
| |
| - | |
| |
| - | |pSB1A2-lox66 as a Forward vector
| |
| - | |
| |
| - | |
| |
| - | |[[Paris/August 13| August 13]]
| |
| - | |- style="background: #cccccc;"
| |
| - | | style="background: #ccffcc;" |D43
| |
| - | |gfp-tripart FI
| |
| - | |MP8.1
| |
| - | |EcoRI
| |
| - | |SpeI
| |
| - | |
| |
| - | |gfp-tri part (strongRBS-ORF-T) ready for use as a forward insert
| |
| - | |
| |
| - | |
| |
| - | |[[Paris/August 13| August 13]]
| |
| - | |- style="background: #cccccc;"
| |
| - | | style="background: #ccffcc;" |D44
| |
| - | |<bbpart>B0015</bbpart> terminator BI
| |
| - | |<bbpart>B0015</bbpart> in <bbpart>pSB1AK3</bbpart> (MP3.3 Aug.14)
| |
| - | |XbaI
| |
| - | |Pst1
| |
| - | |
| |
| - | |terminator B0015 extracted for use as a BI
| |
| - | |
| |
| - | |
| |
| - | |[[Paris/August 16| August 16]]
| |
| - | |- style="background: #cccccc;"
| |
| - | | style="background: #ccffcc;" |D45
| |
| - | |<bbpart>B0015</bbpart> terminator FI
| |
| - | |<bbpart>B0015</bbpart> in <bbpart>pSB1AK3</bbpart> (MP3.4 Aug.14)
| |
| - | |EcoRI
| |
| - | |SpeI
| |
| - | |
| |
| - | |terminator B0015 extracted for use as a FI
| |
| - | |
| |
| - | |
| |
| - | |[[Paris/August 16| August 16]]
| |
| - | |- style="background: #cccccc;"
| |
| - | | style="background: #FF99FF;" |D46
| |
| - | |<bbpart>B0015</bbpart> BV
| |
| - | |<bbpart>B0015</bbpart> in <bbpart>pSB1AK3</bbpart> (MP3.3 Aug.14)
| |
| - | |SpeI
| |
| - | |Pst1
| |
| - | |
| |
| - | |<bbpart>B0015</bbpart> in <bbpart>pSB1AK3</bbpart> plasmid extracted for use as a BV
| |
| - | |
| |
| - | |
| |
| - | |[[Paris/August 16| August 16]]
| |
| - | |- style="background: #cccccc;"
| |
| - | | style="background: #FF99FF;" |D47
| |
| - | |<bbpart>pSB1A2</bbpart> XP dig.
| |
| - | |<bbpart>pSB1A2</bbpart> carrying Cre ORF (MP9.1)
| |
| - | |XbaI
| |
| - | |PstI
| |
| - | |
| |
| - | |<bbpart>pSB1A2</bbpart> open vector ready for cloning of BI
| |
| - | |
| |
| - | |
| |
| - | |[[Paris/August 16| August 16]]
| |
| - | |- style="background: #cccccc;"
| |
| - | | style="background: #FF99FF;" |D48
| |
| - | |<bbpart>pSB1A2</bbpart> ES dig.
| |
| - | |<bbpart>pSB1A2</bbpart> carrying Cre ORF (MP9.2)
| |
| - | |EcoRI
| |
| - | |SpeI
| |
| - | |
| |
| - | |<bbpart>pSB1A2</bbpart> open vector ready for cloning of FI
| |
| - | |
| |
| - | |
| |
| - | |[[Paris/August 16| August 16]]
| |
| - | |- style="background: #cccccc;"
| |
| - | | style="background: #ccffcc;" |D49
| |
| - | |lox71-gfptripart BI
| |
| - | |lox71-gfptripart in <bbpart>pSB1A2</bbpart> (L12.2)
| |
| - | |XbaI
| |
| - | |Pst1
| |
| - | |
| |
| - | |lox71-gfptripart extracted for use as a BI
| |
| - | |
| |
| - | |
| |
| - | |[[Paris/August 16| August 16]]
| |
| - | |- style="background: #cccccc;"
| |
| - | | style="background: #FF99FF;" |D50
| |
| - | |<bbpart>BBa_J61002</bbpart> pTet SP dig.
| |
| - | |<bbpart>BBa_J61002</bbpart> (MP13.1 [[Paris/August 14| Aug. 14]])
| |
| - | |SpeI
| |
| - | |PstI
| |
| - | |
| |
| - | |<bbpart>BBa_J61002</bbpart> pTet promoter vector ready for insertion of CDS as a BI
| |
| - | |
| |
| - | |
| |
| - | |[[Paris/August 16| August 16]]
| |
| - | |- style="background: #cccccc;"
| |
| - | | style="background: #ccffcc;" |D51
| |
| - | |RBS-dapAcoli BBinsert
| |
| - | |pTOPO of RBS-dapAcoli (MPT4.3)
| |
| - | |EcoRI
| |
| - | |Pst1
| |
| - | |
| |
| - | |RBS-dapAcoli extracted for cloning as a biobrick
| |
| - | |
| |
| - | |
| |
| - | |[[Paris/August 16| August 16]]
| |
| - | |- style="background: #cccccc;"
| |
| - | | style="background: #ccffcc;" |D52
| |
| - | |RBS-dapAcoli BI
| |
| - | |pTOPO of RBS-dapAcoli (MPT4.3)
| |
| - | |XbaI
| |
| - | |Pst1
| |
| - | |
| |
| - | |RBS-dapAcoli extracted for use as a BI
| |
| - | |
| |
| - | |
| |
| - | |[[Paris/August 16| August 16]]
| |
| - | |- style="background: #cccccc;"
| |
| - | | style="background: #ccffcc;" |D53
| |
| - | |RBS-dapAsubtilis BBinsert
| |
| - | |pTOPO of RBS-dapAsubtilis (MPT5.1)
| |
| - | |EcoRI
| |
| - | |Pst1
| |
| - | |
| |
| - | |RBS-dapAsubtilis extracted for cloning as a biobrick
| |
| - | |
| |
| - | |
| |
| - | |[[Paris/August 16| August 16]]
| |
| - | |- style="background: #cccccc;"
| |
| - | | style="background: #ccffcc;" |D54
| |
| - | |RBS-dapAsubtilis BI
| |
| - | |pTOPO of RBS-dapAsubtilis (MPT5.1)
| |
| - | |XbaI
| |
| - | |Pst1
| |
| - | |
| |
| - | |RBS-dapAsubtilis extracted for use as a BI
| |
| - | |Ø (24/09/07)
| |
| - | |
| |
| - | |[[Paris/August 16| August 16]]
| |
| - | |- style="background: #cccccc;"
| |
| - | | style="background: #ccffcc;" |D55
| |
| - | |RBS-dapAsubtilis FI
| |
| - | |pTOPO of RBS-dapAsubtilis (MPT5.2)
| |
| - | |EcoRI
| |
| - | |SpeI
| |
| - | |
| |
| - | |RBS-dapAsubtilis extracted for use as a FI
| |
| - | |
| |
| - | |
| |
| - | |[[Paris/August 16| August 16]]
| |
| - | |- style="background: #cccccc;"
| |
| - | | style="background: #ccffcc;" |D57
| |
| - | |lox71-B0015(T) BI
| |
| - | |(L31.1, L31.3) lox71-B0015(T) <bbpart>pSB1A2</bbpart>
| |
| - | |XbaI
| |
| - | |Pst1
| |
| - | |
| |
| - | |lox71-B0015(T) extracted for use as a BI <bbpart>pSB1A2</bbpart>
| |
| - | |
| |
| - | |
| |
| - | |[[Paris/September 14| Sep. 14]]
| |
| - | |- style="background: #cccccc;"
| |
| - | | style="background: #FF99FF;" |D58
| |
| - | |B0015(T)-lox66 BV
| |
| - | |(L32.1, L32.2) B0015(T)-lox66 BV
| |
| - | |SpeI
| |
| - | |PstI
| |
| - | |
| |
| - | |B0015(T)-lox66 for use as a BV <bbpart>pSB1A2</bbpart>
| |
| - | |
| |
| - | |
| |
| - | |[[Paris/September 14| Sep. 14]]
| |
| - | |- style="background: #cccccc;"
| |
| - | | style="background: #ccffcc;" |D59
| |
| - | |pTet>>lox71-gfptripart BI
| |
| - | |(L34.1, L34.2) pTet>>lox71-gfptripart <bbpart>BBa_J61002</bbpart>
| |
| - | |XbaI
| |
| - | |PstI
| |
| - | |
| |
| - | |pTet>>lox71-gfptripart for use as a BI
| |
| - | |
| |
| - | |
| |
| - | |[[Paris/September 14| Sep. 14]]
| |
| - | |- style="background: #cccccc;"
| |
| - | | style="background: #FF99FF;" |D61
| |
| - | |lox66-mRFP FV
| |
| - | |(L36.1, L36.2) lox66-mRFP
| |
| - | |EcoRI
| |
| - | |XbaI
| |
| - | |
| |
| - | |lox66-mRFP part (strongRBS-ORF-T) ready for use as a forward vector
| |
| - | |
| |
| - | |
| |
| - | |[[Paris/September 14| Sep. 14]]
| |
| - | |- style="background: #cccccc;"
| |
| - | | style="background: #ccffcc;" |D62
| |
| - | |lox66-RBSDapAcoli BI
| |
| - | |(L37.1, L37.2) lox66-RBSDapAcoli
| |
| - | |XbaI
| |
| - | |PstI
| |
| - | |
| |
| - | |lox66-RBSDapAcoli ready for use as a Backward insert
| |
| - | |
| |
| - | |
| |
| - | |[[Paris/September 14| Sep. 14]]
| |
| - | |- style="background: #cccccc;"
| |
| - | | style="background: #ccffcc;" |D63
| |
| - | |RBSDapAcoli BI
| |
| - | |(L39.1, L39.2) RBSDapAcoli
| |
| - | |XbaI
| |
| - | |PstI
| |
| - | |
| |
| - | |RBSDapAcoli BI (for insertion behind T-lox66)
| |
| - | |
| |
| - | |
| |
| - | |[[Paris/September 14| Sep. 14]]
| |
| - | |- style="background: #cccccc;"
| |
| - | | style="background: #FF99FF;" |D64
| |
| - | |FRT-CmR-FRT <bbpart>BBa_J61025</bbpart> BV
| |
| - | |(MP14.1) <bbpart>BBa_J61025</bbpart>
| |
| - | |SpeI
| |
| - | |PstI
| |
| - | |
| |
| - | |FRT-CmR-FRT <bbpart>BBa_J61025</bbpart> (for backward introduction of a casette for Wanner K insertion)
| |
| - | |
| |
| - | |
| |
| - | |[[Paris/September 14| Sep. 14]]
| |
| - | |- style="background: #cccccc;"
| |
| - | | style="background: #FF99FF;" |D65
| |
| - | |FRT-CmR-FRT <bbpart>BBa_J61025</bbpart> FV
| |
| - | |(MP14.1) <bbpart>BBa_J61025</bbpart>
| |
| - | |EcoR
| |
| - | |XbaI
| |
| - | |
| |
| - | |FRT-CmR-FRT <bbpart>BBa_J61025</bbpart> (for forward introduction of a casette for Wanner K insertion)
| |
| - | |
| |
| - | |
| |
| - | |[[Paris/September 14| Sep. 14]]
| |
| - | |- style="background: #cccccc;"
| |
| - | | style="background: #FF99FF;" |D68
| |
| - | |attB BV
| |
| - | |(MP15.1)
| |
| - | |SpeI
| |
| - | |PstI
| |
| - | |
| |
| - | |vector containing attB for use as a BV
| |
| - | |
| |
| - | |
| |
| - | |[[Paris/September 14| Sep. 14]]
| |
| - | |- style="background: #cccccc;"
| |
| - | | style="background: #FF99FF;" |D69
| |
| - | |gfptripart-lox66 BV
| |
| - | |(L29.1) gfptripart-lox66 <bbpart>pSB1A2</bbpart>
| |
| - | |SpeI
| |
| - | |PstI
| |
| - | |
| |
| - | |
| |
| - | |
| |
| - | |
| |
| - | |[[Paris/September 14| Sep. 14]]
| |
| - | |- style="background: #cccccc;"
| |
| - | | style="background: #ccffcc;" |D70
| |
| - | |pTet>>lox71-gfptripart FI
| |
| - | |(L34.1, L34.2) pTet>>lox71-gfptripart <bbpart>BBa_J61002</bbpart>
| |
| - | |EcoRI
| |
| - | |SpeI
| |
| - | |
| |
| - | |pTet>>lox71-gfptripart for use as a FI
| |
| - | |
| |
| - | |
| |
| - | |[[Paris/September 14| Sep. 14]]
| |
| - | |- style="background: #cccccc;"
| |
| - | | style="background: #ccffcc;" |D71
| |
| - | |araC/pBad>>lox71-gfptripart FI
| |
| - | |L35.1 araC/pBad>>lox71-gfptripart <bbpart>BBa_J61002</bbpart>
| |
| - | |EcoRI
| |
| - | |SpeI
| |
| - | |
| |
| - | |araC/pBad>>lox71-gfptripart for use as a FI
| |
| - | |Ø (24/09/07)
| |
| - | |
| |
| - | |[[Paris/September 14| Sep. 14]][[Paris/September 17| Sep. 17]]
| |
| - | |- style="background: #cccccc;"
| |
| - | | style="background: #FF99FF;" |D72
| |
| - | |<bbpart>pSB2K3</bbpart> BBvector
| |
| - | |MP16 <bbpart>pSB2K3</bbpart>::attP
| |
| - | |EcoRI
| |
| - | |PstI
| |
| - | |
| |
| - | |Low copy number plasmid, BB vector
| |
| - | |
| |
| - | |
| |
| - | |[[Paris/September 17| Sep. 17]]
| |
| - | |- style="background: #cccccc;"
| |
| - | | style="background: #FF99FF;" |D73
| |
| - | |<bbpart>pSB2K3</bbpart> ES open vector
| |
| - | |MP16 <bbpart>pSB2K3</bbpart>::attP
| |
| - | |EcoRI
| |
| - | |SpeI
| |
| - | |
| |
| - | |Comparison of digestion efficiency between Pst and Spe in NEB2
| |
| - | |
| |
| - | |
| |
| - | |[[Paris/September 17| Sep. 17]]
| |
| - | |- style="background: #cccccc;"
| |
| - | | style="background: #ccffcc;" |D76
| |
| - | |DGAT BB
| |
| - | |P14 DGAT
| |
| - | |EcoRI
| |
| - | |PstI
| |
| - | |
| |
| - | |DGAT
| |
| - | |Ø (24/09/07)
| |
| - | |
| |
| - | |[[Paris/September 17| Sep. 17]]
| |
| - | |- style="background: #cccccc;"
| |
| - | | style="background: #ccffcc;" |D77
| |
| - | |araC/pBad>>lox71-gfptripart BI
| |
| - | |D60 (.1 & .2) digestion product
| |
| - | |FspI
| |
| - | |AhdI
| |
| - | |
| |
| - | |in D60, the extracted fragment & the open plasmid have the same size, it is thus necessary to remove the plasmid by digestion
| |
| - | |
| |
| - | |
| |
| - | |[[Paris/September 17| Sep. 17]]
| |
| - | |- style="background: #cccccc;"
| |
| - | | style="background: #ccffcc;" |D78
| |
| - | |araC/pBad>>lox71-gfptripart FI
| |
| - | |D71 (.1 & .2) digestion product
| |
| - | |FspI
| |
| - | |AhdI
| |
| - | |
| |
| - | |in D71, the extracted fragment & the open plasmid have the same size, it is thus necessary to remove the plasmid by digestion
| |
| - | |
| |
| - | |
| |
| - | |[[Paris/September 17| Sep. 17]]
| |
| | |} | | |} |
| | | | |
| Line 1,122: |
Line 72: |
| | |width=55%| Description | | |width=55%| Description |
| | |width=5%| Date | | |width=5%| Date |
| - | |- style="background: #cccccc;"
| |
| - | | style="background: #ccffcc;" |D16
| |
| - | |<bbpart>pSB1AK3</bbpart>::<bbpart>B0015</bbpart> (MP3.1 & MP3.2)
| |
| - | |EcoR1
| |
| - | |Pst1
| |
| - | |~3000
| |
| - | |We have one band at ~2000. Furthermore, the strain which is suppose to be resistant to Kanamycin is not. We suspect that the part is in the pSB1A2 instead of the pSB1AK3
| |
| - | |[[Paris/July 21| July 21]]
| |
| - | |- style="background: #cccccc;"
| |
| - | | style="background: #ccffcc;" |D17
| |
| - | |<bbpart>pSB1A2</bbpart>::<bbpart>E0422</bbpart> (MP6.1 & MP6.2)
| |
| - | |EcoRI
| |
| - | |PstI
| |
| - | |~2040 + ~920
| |
| - | |3 bands
| |
| - | |[[Paris/July 21| July 21]]
| |
| - | |- style="background: #cccccc;"
| |
| - | | style="background: #ccffcc;" |D25
| |
| - | |<bbpart>BBa_E0421</bbpart> (MP7.1 & MP7.2) PoPs to GFP converter
| |
| - | |XbaI
| |
| - | |PstI
| |
| - | |
| |
| - | |
| |
| - | |[[Paris/July 23| July 23]]
| |
| - | |- style="background: #cccccc;"
| |
| - | | style="background: #FF99FF;" |D66
| |
| - | |(MP12.1, MP12.2) <bbpart>BBa_P1003</bbpart> in <bbpart>pSB1A2</bbpart>
| |
| - | |EcoR
| |
| - | |XbaI
| |
| - | |
| |
| - | |KanR <bbpart>BBa_J61025</bbpart> FV (for forward introduction of a casette for Wanner K insertion). The strange thing is that this digestion produces 2 visible products: ~1000 bp & ~2500 bp!!!
| |
| - | |[[Paris/September 17| Sep.17]], [[Paris/September 27| Sep.27]]
| |
| | |} | | |} |
| | | | |
| Line 1,166: |
Line 84: |
| | |width=5%| Verified clone | | |width=5%| Verified clone |
| | |width=5%| Date | | |width=5%| Date |
| - | |- style="background: #cccccc;"
| |
| - | | style="background: #ccffcc;" |S1
| |
| - | |MG1655
| |
| - | |Standard E.Coli strain
| |
| - | |
| |
| - | |
| |
| - | |- style="background: #cccccc;"
| |
| - | | style="background: #ccffcc;" |S2
| |
| - | |Acinetobacter ADP1
| |
| - | |Strain used for TG coloration test with Red Nile
| |
| - | |
| |
| - | |
| |
| - | |- style="background: #cccccc;"
| |
| - | | style="background: #ccffcc;" |S3
| |
| - | |DH5alpha pKs::DGAT
| |
| - | |Enzyme catalysing the transormation of DAG into TG
| |
| - | |
| |
| - | |
| |
| - | |- style="background: #cccccc;"
| |
| - | | style="background: #ccffcc;" |S4
| |
| - | |<bbpart>BBa_pJ23100</bbpart>
| |
| - | |Strong constitutive promoter in J61002 (clones 1 + 2) <partinfo>pJ23100 SpecifiedComponents</partinfo>
| |
| - | |
| |
| - | |
| |
| - | |- style="background: #cccccc;"
| |
| - | | style="background: #ccffcc;" |S5
| |
| - | |<bbpart>BBa_B0015</bbpart>
| |
| - | |Double terminator (clones 1 + 2) <partinfo>B0015 SpecifiedComponents</partinfo>
| |
| - | |
| |
| - | |
| |
| - | |- style="background: #cccccc;"
| |
| - | | style="background: #ccffcc;" |S6
| |
| - | |<bbpart>BBa_pJ23107</bbpart>
| |
| - | |Medium constitutive promoter in J61002 (clones 1 + 2) <partinfo>pJ23107 SpecifiedComponents</partinfo>
| |
| - | |
| |
| - | |[[Paris/July 19| July 19]]
| |
| - | |- style="background: #cccccc;"
| |
| - | | style="background: #ccffcc;" |S7
| |
| - | |<bbpart>BBa_B0030</bbpart>
| |
| - | |Strong RBS (clones 1 + 2) <partinfo>B0030 SpecifiedComponents</partinfo>
| |
| - | |
| |
| - | |[[Paris/July 19| July 19]]
| |
| - | |- style="background: #cccccc;"
| |
| - | | style="background: #ccffcc;" |S8
| |
| - | |<bbpart>BBa_E0422</bbpart>
| |
| - | |ECFP (RBS+LVA+Term) (clones 1 + 2) <partinfo>E0422 SpecifiedComponents</partinfo>
| |
| - | |
| |
| - | |[[Paris/July 19| July 19]]
| |
| - | |- style="background: #cccccc;"
| |
| - | | style="background: #ccffcc;" |S9
| |
| - | |<bbpart>BBa_E0241</bbpart>
| |
| - | |PoPs to GFP converter (clones 1 + 2) <partinfo>E0241 DeepComponents</partinfo>
| |
| - | |
| |
| - | |[[Paris/July 19| July 19]]
| |
| - | |- style="background: #cccccc;"
| |
| - | | style="background: #ccffcc;" |S10
| |
| - | |<bbpart>BBa_E0840</bbpart>
| |
| - | |gfp tri-part; strong rbs (clones 1 + 2) <partinfo>E0840 SpecifiedComponents</partinfo>
| |
| - | |
| |
| - | |[[Paris/July 19| July 19]]
| |
| - | |- style="background: #cccccc;"
| |
| - | | style="background: #ccffcc;" |S11
| |
| - | |<bbpart>BBa_J61047</bbpart>
| |
| - | |Cre ORF (clones 1 + 2) <partinfo>J61047 SpecifiedComponents</partinfo>
| |
| - | |
| |
| - | |[[Paris/July 19| July 19]]
| |
| - | |- style="background: #cccccc;"
| |
| - | | style="background: #ccffcc;" |S12
| |
| - | |W121
| |
| - | |
| |
| - | |
| |
| - | |05/07/07
| |
| - | |- style="background: #cccccc;"
| |
| - | | style="background: #ccffcc;" |S13
| |
| - | |<bbpart>BBa_I0500</bbpart>
| |
| - | |pBAD AraC (clone 1+2) <partinfo>I0500 SpecifiedComponents</partinfo>
| |
| - | |
| |
| - | |[[Paris/July 20| July 20]]
| |
| - | |- style="background: #cccccc;"
| |
| - | | style="background: #ccffcc;" |S14
| |
| - | |<bbpart>BBa_pJ23107</bbpart>
| |
| - | |Medium constitutive promoter in J61002 (clone 3+4) <partinfo>pJ23107 SpecifiedComponents</partinfo>
| |
| - | |
| |
| - | |[[Paris/July 20| July 20]]
| |
| - | |- style="background: #cccccc;"
| |
| - | | style="background: #ccffcc;" |S15
| |
| - | |<bbpart>BBa_P1003</bbpart>
| |
| - | |Kanamycin resistance cassette (clone 1+2) <partinfo>P1003 SpecifiedComponents</partinfo>
| |
| - | |
| |
| - | |[[Paris/July 20| July 20]]
| |
| - | |- style="background: #cccccc;"
| |
| - | | style="background: #ccffcc;" |S16
| |
| - | |<bbpart>J61002</bbpart>::<bbpart>I0500</bbpart>
| |
| - | |AraC pBad regulating mRFP expression in pJ61002 (L1) <partinfo>J61002 SpecifiedComponents</partinfo>
| |
| - | |L1.2, L1.3
| |
| - | |[[Paris/July 27| July 27]]
| |
| - | |- style="background: #cccccc;"
| |
| - | | style="background: #ccffcc;" |S17
| |
| - | |<bbpart>pSB1A2</bbpart>::Lox66-DapA Coli
| |
| - | |Lox66-DapA Coli cloned as a biobrick (L2)
| |
| - | |
| |
| - | |[[Paris/July 27| July 27]]
| |
| - | |- style="background: #cccccc;"
| |
| - | | style="background: #ccffcc;" |S18
| |
| - | |<bbpart>pSB1A2</bbpart>::B0030 + J61047
| |
| - | |RBS(B0030)-Cre ORF (L6) (clones 1+2)
| |
| - | |
| |
| - | |[[Paris/July 27| July 27]]
| |
| - | |- style="background: #cccccc;"
| |
| - | | style="background: #ccffcc;" |S19
| |
| - | |<bbpart>pSB1A2</bbpart>::<bbpart>BBa_J37026</bbpart>
| |
| - | |Lox66 (L9)(1 clone) <partinfo>J37026 SpecifiedComponents</partinfo>
| |
| - | |
| |
| - | |[[Paris/July 27| July 27]]
| |
| - | |- style="background: #cccccc;"
| |
| - | | style="background: #ccffcc;" |S20
| |
| - | |<bbpart>pSB1A2</bbpart>::<bbpart>BBa_J37028</bbpart>
| |
| - | |Lox71 (L10)(1 clone) <partinfo>J37028 SpecifiedComponents</partinfo>
| |
| - | |
| |
| - | |[[Paris/July 27| July 27]]
| |
| - | |- style="background: #cccccc;"
| |
| - | | style="background: #ccffcc;" |S21
| |
| - | |<bbpart>pSB1A2</bbpart>::<bbpart>BBa_J37028</bbpart>+<bbpart>BBa_E0840</bbpart>
| |
| - | |Lox71-gfp tri-part (L12)
| |
| - | |L12.2 ok
| |
| - | |?
| |
| - | |- style="background: #cccccc;"
| |
| - | | style="background: #ccffcc;" |S22
| |
| - | |<bbpart>pSB1A2</bbpart>::Lox71-kmR-lox66
| |
| - | |Lox71-kmR-lox66 (L25)
| |
| - | |sequence not good (thrown away)
| |
| - | |?
| |
| - | |- style="background: #cccccc;"
| |
| - | | style="background: #ccffcc;" |S23
| |
| - | |pTopo::P5
| |
| - | |Lox66-DapAColi
| |
| - | |
| |
| - | |?
| |
| - | |- style="background: #cccccc;"
| |
| - | | style="background: #ccffcc;" |S24
| |
| - | |pTopo::P6
| |
| - | |Lox66-DapASubtilis
| |
| - | |
| |
| - | |?
| |
| - | |- style="background: #cccccc;"
| |
| - | | style="background: #ccffcc;" |S25
| |
| - | |pTopo::P8
| |
| - | |RBS-DapAColi
| |
| - | |MPT4.3, MPT4.5
| |
| - | |?
| |
| - | |- style="background: #cccccc;"
| |
| - | | style="background: #ccffcc;" |S26
| |
| - | |pTopo::P9
| |
| - | |RBS-DapASubtilis
| |
| - | |
| |
| - | |?
| |
| - | |- style="background: #cccccc;"
| |
| - | | style="background: #ccffcc;" |S27
| |
| - | |<bbpart>J61002</bbpart>
| |
| - | |
| |
| - | |
| |
| - | |[[Paris/August 14| August 14]]
| |
| - | |- style="background: #cccccc;"
| |
| - | | style="background: #ccffcc;" |S28
| |
| - | |<bbpart>J61025</bbpart>
| |
| - | |FRT-Cm-FRT
| |
| - | |
| |
| - | |[[Paris/August 14| August 14]]
| |
| - | |- style="background: #cccccc;"
| |
| - | | style="background: #ccffcc;" |S29
| |
| - | |L26
| |
| - | |Insertion of RBS-Cre behind araC/pBad
| |
| - | |
| |
| - | |[[Paris/August 13| August 13]]
| |
| - | |- style="background: #cccccc;"
| |
| - | | style="background: #ccffcc;" |S30
| |
| - | |L27
| |
| - | |Insertion of ftsZ behind araC/pBad
| |
| - | |
| |
| - | |[[Paris/August 13| August 13]]
| |
| - | |- style="background: #cccccc;"
| |
| - | | style="background: #ccffcc;" |S31
| |
| - | |L28
| |
| - | |Insertion of ftsAZ behind araC/pBad
| |
| - | |
| |
| - | |[[Paris/August 13| August 13]]
| |
| - | |- style="background: #cccccc;"
| |
| - | | style="background: #ccffcc;" |S32
| |
| - | |L29
| |
| - | |Insertion of gfp-tripart in front of lox66
| |
| - | |
| |
| - | |[[Paris/August 13| August 13]]
| |
| - | |- style="background: #cccccc;"
| |
| - | | style="background: #ccffcc;" |S33A
| |
| - | |L30A
| |
| - | |cloning dapAp promotor in J61002
| |
| - | |
| |
| - | |[[Paris/August 13| August 13]]
| |
| - | |- style="background: #cccccc;"
| |
| - | | style="background: #ccffcc;" |S33B
| |
| - | |L30B
| |
| - | |cloning dapAp promotor in J61002
| |
| - | |
| |
| - | |[[Paris/August 13| August 13]]
| |
| - | |- style="background: #cccccc;"
| |
| - | | style="background: #ccffcc;" |S34
| |
| - | |L31
| |
| - | |Insertion of B0015 terminator behind lox71
| |
| - | |
| |
| - | |[[Paris/August 22| August 22]]
| |
| - | |- style="background: #cccccc;"
| |
| - | | style="background: #ccffcc;" |S35
| |
| - | |L32
| |
| - | |Insertion of B0015 terminator in front of lox66
| |
| - | |
| |
| - | |[[Paris/August 22| August 22]]
| |
| - | |- style="background: #cccccc;"
| |
| - | | style="background: #ccffcc;" |S36
| |
| - | |L34
| |
| - | |pTet>>lox71-gfptripart
| |
| - | |
| |
| - | |[[Paris/August 22| August 22]]
| |
| - | |- style="background: #cccccc;"
| |
| - | | style="background: #ccffcc;" |S37
| |
| - | |L35
| |
| - | |araC/pBad>>lox71-gfptripart
| |
| - | |
| |
| - | |[[Paris/August 22| August 22]]
| |
| - | |- style="background: #cccccc;"
| |
| - | | style="background: #ccffcc;" |S38
| |
| - | |L36
| |
| - | |lox66-mRFP
| |
| - | |
| |
| - | |[[Paris/August 22| August 22]]
| |
| - | |- style="background: #cccccc;"
| |
| - | | style="background: #ccffcc;" |S39
| |
| - | |L37
| |
| - | |lox66-RBS-dapAcoli BI
| |
| - | |
| |
| - | |[[Paris/August 22| August 22]]
| |
| - | |- style="background: #cccccc;"
| |
| - | | style="background: #ccffcc;" |S40
| |
| - | |L39
| |
| - | |RBS-dapAcoli cloned in pSB1A2
| |
| - | |
| |
| - | |[[Paris/August 22| August 22]]
| |
| - | |- style="background: #cccccc;"
| |
| - | | style="background: #ccffcc;" |S41
| |
| - | |L40
| |
| - | |RBS-dapAsubtilis cloned in pSB1A2
| |
| - | |
| |
| - | |[[Paris/August 22| August 22]]
| |
| - | |- style="background: #cccccc;"
| |
| - | | style="background: #ccffcc;" |S42
| |
| - | |L41
| |
| - | |lox71-ftsZ cloned in pSB1A2
| |
| - | |
| |
| - | |[[Paris/August 22| August 22]]
| |
| - | |- style="background: #cccccc;"
| |
| - | | style="background: #ccffcc;" |S43
| |
| - | |L42
| |
| - | |lox71-ftsA-Z cloned in pSB1A2
| |
| - | |
| |
| - | |[[Paris/August 22| August 22]]
| |
| - | |- style="background: #cccccc;"
| |
| - | | style="background: #ccffcc;" |S44
| |
| - | |L43
| |
| - | |RBS-dapAsubtilis cloned in pSB1A2
| |
| - | |
| |
| - | |[[Paris/August 22| August 22]]
| |
| - | |- style="background: #cccccc;"
| |
| - | | style="background: #ccffcc;" |S45A
| |
| - | |L30A
| |
| - | |DapAp>>mRFP within W121 strain
| |
| - | |
| |
| - | |[[Paris/August 25| August 25]]
| |
| - | |- style="background: #cccccc;"
| |
| - | | style="background: #ccffcc;" |S45B
| |
| - | |L30B
| |
| - | |DapAp>>mRFP within W121 strain
| |
| - | |
| |
| - | |[[Paris/August 25| August 25]]
| |
| - | |- style="background: #cccccc;"
| |
| - | | style="background: #ccffcc;" |S48
| |
| - | |pKD13
| |
| - | |FRT-KanR-FRT; Wanner plasmid
| |
| - | |
| |
| - | |[[Paris/September 18| Sep. 18]]
| |
| - | |- style="background: #cccccc;"
| |
| - | | style="background: #ccffcc;" |S49
| |
| - | |pKD46
| |
| - | |Red recombination system; Wanner plasmid
| |
| - | |
| |
| - | |[[Paris/September_13| Sep. 13]]
| |
| - | |- style="background: #cccccc;"
| |
| - | | style="background: #ccffcc;" |S50
| |
| - | |L30 plasmid in FR781
| |
| - | |dapAp>>mRFP plasmid
| |
| - | |
| |
| - | |[[Paris/September_28| Sep. 28]]
| |
| | |} | | |} |
"
