Team:Paris/Protocols

From 2009.igem.org

(Difference between revisions)
(Protocols)
(Protocols)
Line 8: Line 8:
content :
content :
-
Growing bacteria in liquid medium
+
*Growing bacteria in liquid medium
-
Preparing growth media
+
*Preparing growth media
-
exemple:Making 10 petri dish (LB+erythromycin+citrate+DAP)
+
**exemple:Making 10 petri dish (LB+erythromycin+citrate+DAP)<br>
-
                Solid M9 Minimum Medium
+
Solid M9 Minimum Medium<br>
Preparation de gel d'agarose
Preparation de gel d'agarose
-
Chemical transformation
+
*Chemical transformation
-
Glycerol Stock
+
*Glycerol Stock
-
Recombineering/Lambda red-mediated gene replacement
+
*Recombineering/Lambda red-mediated gene replacement
-
Miniprep
+
*Miniprep
-
Fluorescent single cells visualisation
+
*Fluorescent single cells visualisation
-
Digestion reactions
+
*Digestion reactions

Revision as of 09:13, 23 July 2009

Protocols

iGEM paris 2007 protocols link :

content :

  • Growing bacteria in liquid medium
  • Preparing growth media
    • exemple:Making 10 petri dish (LB+erythromycin+citrate+DAP)

Solid M9 Minimum Medium
Preparation de gel d'agarose

  • Chemical transformation
  • Glycerol Stock
  • Recombineering/Lambda red-mediated gene replacement
  • Miniprep
  • Fluorescent single cells visualisation
  • Digestion reactions