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Team:Paris/Protocols
From 2009.igem.org
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Here you will find the collection of protocole we use, or just collect. And because we are well-known chauvinist it is just a tribute to previous Paris iGEM team . | Here you will find the collection of protocole we use, or just collect. And because we are well-known chauvinist it is just a tribute to previous Paris iGEM team . | ||
| - | + | We try our best not to make it redondant. | |
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<u>content :</u> | <u>content :</u> | ||
| + | |||
| + | *Electrophoresis | ||
| + | *Concentration of the Miniprep or the Midiprep | ||
| + | *Amplification of promoters. | ||
| + | *PCR Screening | ||
| + | *Protocol to make competent bacteria | ||
Revision as of 09:40, 23 July 2009
Protocols
Here you will find the collection of protocole we use, or just collect. And because we are well-known chauvinist it is just a tribute to previous Paris iGEM team . We try our best not to make it redondant.
iGEM paris 2007 protocols link :
[http://parts.mit.edu/igem07/index.php/Paris/PROTOCOLS Click here]
content :
- Growing_bacteria_in_liquid_medium
- Preparing growth media
- exemple: Making 10 petri dish (LB+erythromycin+citrate+DAP), Solid M9 Minimum Medium, preparation of agarosis gel
- Chemical transformation
- Glycerol Stock
- Recombineering/Lambda red-mediated gene replacement
- Miniprep
- Fluorescent single cells visualisation
- Digestion reactions
iGEM paris 2008 protocols link :
[http://parts.mit.edu/igem07/index.php/Paris/PROTOCOLS Click here]
content :
- Electrophoresis
- Concentration of the Miniprep or the Midiprep
- Amplification of promoters.
- PCR Screening
- Protocol to make competent bacteria
