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EPF-Lausanne/28 July 2009
From 2009.igem.org
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==Wet Lab== | ==Wet Lab== | ||
| + | LacI, RBS and the death cassette were digested with E/S, X/P and E/P respectively. (Incubated at 37°C for 1h30) The digestion products have been purified, and than ligated (incubation at RT for 3 hours). | ||
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| + | The digestion products have been runned on a gel to see if the digestion worked, it worked for the death cassette but we are not able to see the LacI bands and the RBS bands. | ||
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| + | LOVTAP-term ligation products and LacI-RBS ligation products have been transformed in DH5-alpha. | ||
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| + | Finally RBS plasmid has been retransformed to have RBS part again. | ||
==People in the lab== | ==People in the lab== | ||
:Gab, Christian, Mélanie, Nath | :Gab, Christian, Mélanie, Nath | ||
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Latest revision as of 09:26, 29 July 2009
Contents |
Wet Lab
LacI, RBS and the death cassette were digested with E/S, X/P and E/P respectively. (Incubated at 37°C for 1h30) The digestion products have been purified, and than ligated (incubation at RT for 3 hours).
The digestion products have been runned on a gel to see if the digestion worked, it worked for the death cassette but we are not able to see the LacI bands and the RBS bands.
LOVTAP-term ligation products and LacI-RBS ligation products have been transformed in DH5-alpha.
Finally RBS plasmid has been retransformed to have RBS part again.
People in the lab
- Gab, Christian, Mélanie, Nath
