Team:Paris/Protocols PCRqload
From 2009.igem.org
(Difference between revisions)
(→PCR with Quick load Taq2x Master Mix) |
(→PCR with Quick load Taq2x Master Mix) |
||
Line 3: | Line 3: | ||
==PCR with Quick load Taq2x Master Mix== | ==PCR with Quick load Taq2x Master Mix== | ||
- | |||
*Mix for final volume of 50µl: | *Mix for final volume of 50µl: | ||
**14µL H2O | **14µL H2O |
Revision as of 00:02, 31 July 2009
PCR with Quick load Taq2x Master Mix
- Mix for final volume of 50µl:
- 14µL H2O
- 25µL Taq Quick load Master Mix 2X
- 5µL Oligo Fw (0,2µM)
- 5µL Oligo Rw (0,2µM)
- 1µL Matrice (DNA)