Team:Paris/5 August 2009
From 2009.igem.org
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|width=10%| n°9 | |width=10%| n°9 | ||
|width=10%| n°10 | |width=10%| n°10 | ||
+ | |width=10%| n°13 | ||
+ | |width=10%| n°14 | ||
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|width=10%| - | |width=10%| - | ||
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+ | |width=10%| 500µl MgSO4 | ||
+ | |width=10%| 500µl Medium | ||
|- style="background: #E0E9EF; text-align: center;" | |- style="background: #E0E9EF; text-align: center;" | ||
|width=10%| 1µl de colorant | |width=10%| 1µl de colorant | ||
|width=10%| 1µl de colorant | |width=10%| 1µl de colorant | ||
+ | |width=10%| 1µl | ||
+ | |width=10%| 1µl | ||
|width=10%| 1µl | |width=10%| 1µl | ||
|width=10%| 1µl | |width=10%| 1µl | ||
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|width=10%| incubation 60min 37°C | |width=10%| incubation 60min 37°C | ||
|width=10%| incubation 30min 37°C | |width=10%| incubation 30min 37°C | ||
+ | |width=10%| incubation 60min 37°C | ||
+ | |width=10%| incubation 60min 37°C | ||
|width=10%| incubation 60min 37°C | |width=10%| incubation 60min 37°C | ||
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|width=10%| depot sur lame | |width=10%| depot sur lame | ||
+ | |width=10%| - | ||
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|width=10%| nothing | |width=10%| nothing | ||
|width=10%| some cell fluo | |width=10%| some cell fluo | ||
+ | |width=10%| noise | ||
+ | |width=10%| noise | ||
|width=10%| noise | |width=10%| noise | ||
|width=10%| noise | |width=10%| noise |
Revision as of 15:09, 5 August 2009
Contents |
NoteBook
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Brain work
edit...
Lab work
Fm4-64 dying | ||||||||||
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n°1 | n°2 | n°3 | n°4 | |||||||
500µl cells | - | - | - | |||||||
centri 4000rpm 3min | - | - | - | |||||||
resuspension 500µl MgSO4 | - | - | - | |||||||
1µl de colorant | 1µl de colorant | 2µl | 2µl | |||||||
incubation 30min 37°C | incubation 60min 37°C | incubation 30min 37°C | incubation 60min 37°C | |||||||
depot sur lame | - | - | - | |||||||
nothing | all cell fluo | too much dye | too much dye |
Fm4-64 dying | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|
n°5 | n°6 | n°9 | n°10 | n°13 | n°14 | |||||
500µl cells | - | - | - | 500µl MgSO4 | 500µl Medium | |||||
1µl de colorant | 1µl de colorant | 1µl | 1µl | 1µl | 1µl | |||||
incubation 30min 37°C | incubation 60min 37°C | incubation 30min 37°C | incubation 60min 37°C | incubation 60min 37°C | incubation 60min 37°C | |||||
centri 4000rpm 3min | - | - | - | |||||||
resuspension 500µl MgSO4 | resuspension 500µl MgSO4 | + filtrage | + filtrage | |||||||
depot sur lame | - | - | - | - | - | |||||
nothing | some cell fluo | noise | noise | noise | noise |
To do list
Matricule | TODO |
Luc | bacteria coloration microscope protocol |
Romain | Look for his feet |
Charlotte | bibliography on SNAREs/Autotransporters/Jun and Fos complex |
Stoff | merge algo protocol/ Wiki |
Chris | modeling: TeTR |
Lisa | WTF !!! |
Caroline | bacteria coloration microscope protocol |
Souf | if(!oligo){return Wiki;} return PCR; |
Vicard | Lab : Gel / purification / insers |
Pierre | Alice / Tol/Pal modelisation / geophysics |
Sylvain | if(!oligo){return Maltose;} return PCR; |
Guillaume | Miniprep for P(1/2/3/4/5/7/8) / New P7 digestion? / Glycerol Stock (DH5α/Kaio strains) |