Team:Paris/21 July 2009
From 2009.igem.org
(Difference between revisions)
Christophe.R (Talk | contribs) (→NoteBook) |
Christophe.R (Talk | contribs) (→Lab work) |
||
(2 intermediate revisions not shown) | |||
Line 14: | Line 14: | ||
</html> | </html> | ||
- | ==Brain work== | + | ===Brain work=== |
- | + | ||
Waiting for the return of Mr. Labs and his signature for the commisions. | Waiting for the return of Mr. Labs and his signature for the commisions. | ||
- | |||
Meeting at 14: | Meeting at 14: | ||
Line 35: | Line 33: | ||
</html> | </html> | ||
- | ==Lab work== | + | ===Lab work=== |
- | *Medium for [[https://2009.igem.org/Team:Paris/ | + | *Medium for [[https://2009.igem.org/Team:Paris/Freezer_Strains_(Glycerol_stock)#S1 S1]] DH5alpha and [[https://2009.igem.org/Team:Paris/Freezer_Strains_(Glycerol_stock)#S2 S2]] K12 MG 1655 delta recA::Kan |
- | ** 5ml of LB + Clone of [S1 | + | ** 5ml of LB + Clone of [S1] |
- | ** 5ml of LB + 5µl of Kan 1000x + Clone of [S2 | + | ** 5ml of LB + 5µl of Kan 1000x + Clone of [S2] |
Overnight at 37°C, 200rpm | Overnight at 37°C, 200rpm | ||
Line 49: | Line 47: | ||
</html> | </html> | ||
- | ==To do list== | + | ===To do list=== |
*Glycerol stock for [S1] and [S2] | *Glycerol stock for [S1] and [S2] | ||
+ | |||
+ | |||
+ | {{Paris2009_Calendar_Link|20_July_2009|22_July_2009}} |
Latest revision as of 19:08, 9 August 2009
Contents |
NoteBook
|
|
|
|
|
---|
Brain work
Waiting for the return of Mr. Labs and his signature for the commisions.
Meeting at 14:
- redistribution of the role and global progress :
- g3p: raiders of the lost phage ... We will maybe amplify the g3p by ourself with a phage m13.
- snare: there is still hope with the future plasmide to come.
- fecA: demanding construction but still in slow progression
- TMG: new idea to bypass our transduction problem .... let's say that the research begin.
Lab work
- Medium for [S1] DH5alpha and [S2] K12 MG 1655 delta recA::Kan
- 5ml of LB + Clone of [S1]
- 5ml of LB + 5µl of Kan 1000x + Clone of [S2]
Overnight at 37°C, 200rpm