Team:Paris/19 August 2009
From 2009.igem.org
(Difference between revisions)
Cha.olivier (Talk | contribs) (→Lab work) |
Cha.olivier (Talk | contribs) (→Lab work) |
||
Line 39: | Line 39: | ||
</div> | </div> | ||
<div class="experience"> | <div class="experience"> | ||
+ | |||
5 clones pLac/RBS-TetR have to be sequenced | 5 clones pLac/RBS-TetR have to be sequenced | ||
Line 52: | Line 53: | ||
</div> | </div> | ||
<div class="experience"> | <div class="experience"> | ||
+ | |||
pSB1A3-TolRII ligation was transformed into P10 chemical competent cells, using the "heat shock" protocol | pSB1A3-TolRII ligation was transformed into P10 chemical competent cells, using the "heat shock" protocol | ||
Line 63: | Line 65: | ||
</div> | </div> | ||
<div class="experience"> | <div class="experience"> | ||
+ | |||
<u>'''''Glycerol stock of ''''':</u> | <u>'''''Glycerol stock of ''''':</u> |
Revision as of 12:17, 19 August 2009
Contents |
NoteBook
|
|
|
|
|
---|
Brain work
edit please ^^
Lab work
Sequencing w/ Bobode
5 clones pLac/RBS-TetR have to be sequenced
Clone 1, 2, 6, 7, 8 used (because of their high DNA concentration)
Transformation w/ Bobode
pSB1A3-TolRII ligation was transformed into P10 chemical competent cells, using the "heat shock" protocol
Glycerol stock w/ Charlie
Glycerol stock of :
- pINTE3 (Col E3, AmpR)
- pTA (pTet-Col A, AmpR)
- pINTg3p (g3p Term, AmpR)
- pINK2(TolRII, AmpR)
To do list
edit please ^^