Team:Paris/Production overview strategy

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Overview

Introduction - Tol/pal - Our strategy - Construction

B. Our strategy

The first strategy is the knock out of tol/pal gene witch destabilize the membrane .It is an active system, whereas the use of conditional mutant is a passive system. A previous study [2] focused on the development of a gene expression system able to induce production of large amounts of OMVs.

To achieve our goal we decided to take advantage of the second domain of TolR (TolRII). We don’t use the third domain of TolA because, it doesn’t work so good and TolAIII have lot of PstI domain in his sequence. In order to achieve our project, we will overexpress specifically designed biobricks containing TolRII fused with OmpA signal which allows it to migrate in the periplasm.[3]

In the same framework, we could also overexpress various Tol ligand (colicin) to destabilize the membrane.

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+===B. Our strategy===
+The first strategy is the knock out of tol/pal gene witch destabilize the membrane .It is an active system, whereas the use of conditional mutant is a passive system. A previous study [2] focused on the development of a gene expression system able to induce production of large amounts of OMVs.
+To achieve our goal we decided to take advantage of the second domain of TolR (TolRII). We don’t use the third domain of TolA because, it doesn’t work so good and TolAIII have lot of PstI domain in his sequence. In order to achieve our project, we will overexpress specifically designed biobricks containing TolRII fused with OmpA signal which allows it to migrate in the periplasm.[3]
+In the same framework, we could also overexpress various Tol ligand (colicin) to destabilize the membrane.