Team:UNICAMP-Brazil/Notebooks/July 25

From 2009.igem.org

(Difference between revisions)
(Primer design)
 
(One intermediate revision not shown)
Line 8: Line 8:
====Primer design====
====Primer design====
-
These are the primers that we´re going to use in out project. How long will it take until they´re sent to us?? :)
+
These are the primers that we are going to use in our project. How long will it take until they´re sent to us?? :)
Line 54: Line 54:
GgLYSO_F TTCTAGAATGAGGTCTTTGCTAATCTTGG  
GgLYSO_F TTCTAGAATGAGGTCTTTGCTAATCTTGG  
-
GgLYSO_R CTACTAGTTTTTAATGAGGGATGCGTTT  
+
GgLYSO_R CTACTAGTTTTTAATGAGGGATGCGTTT  
Cre-F TCCAATTTACTGACCGTACACC
Cre-F TCCAATTTACTGACCGTACACC
-
FSTK_UP_VF2 CACGGAACAGGTGCAAAATCGGCGTATTTTGATTACACTCCTTGCCACCTGACGTCTAAGAA
+
FSTK_UP_VF2 CACGGAACAGGTGCAAAATCGGCGTATTTTGATTACACTCCTTGCCACCTGACGTCTAAGAA
FSTK_UP_VR TACAGGCTTTTCCTGCCCGATGCTAAAACGGACAACAGTACCATTACCGCCTTTGAGTGAGC  
FSTK_UP_VR TACAGGCTTTTCCTGCCCGATGCTAAAACGGACAACAGTACCATTACCGCCTTTGAGTGAGC  

Latest revision as of 16:51, 7 September 2009

Topo l2.gif topo_r_igem.gif
topo_r_b.gif
Back to Calendar

MicroGuards

Primer design

These are the primers that we are going to use in our project. How long will it take until they´re sent to us?? :)


Col-R CTACTAGTACCAGAAATTCAGCCTCGGTA

Anticol-F TTCTAGAGGTGGTAGGGAACGCTTTGAA

Anticol-R CTACTAGTAACCGGCATTCATATTTCAGC

HLY-F TTCTAGAGATAAAGATGGTCGAGA

HLY-R CTACTAGTCTAACGTTCACGTAAACTT

LYS-F TTCTAGAATGGTTGAAATCAATAATC

LYS-R CTACTAGTTACATCAATCTCTCTGACC

FinOF-XbaI TTCTAGAGATGACAGAGCAGAAACGACCGG

FinOR-SpeI CTACTAGTATTATTTCTCATCAAGCACGGCC

FinPF-XbaI TTCTAGAGTGACGAGCATGTTTTTGTTGAA

FinPR-SpeI CTACTAGTAAAAATCGCCGATGCAGGGAGAC

Ppy-F-1 TTCTAGAGACGAGAAGGCTATGTGTATCA

Ppy-F-2 TTCTAGAGTTTCCGTCTATTTACCTTTTCTG

Ppy-R CTACTAGTATAACACCTCCCGCTGTTTAT

KlJEN1-F TTCTAGATCAACCATGAATAACAACAAT

KlJEN1-R CTACTAGTATAATGCTATGGTGCGTGT

Prom-KlJEN1-F TTCTAGATGATTGGCAAACCTGTTGT

Prom-KlJEN1-R CTACTAGTGGTTGAAATCTGTTTTGGTTTT

Prom-KlDLD-F TTCTAGAGGTCGCTCTTTCTCTCTCTCAT

Prom-KlDLD-R CTACTAGTTGAGTTGCGTTTCTGGTCTTT

GgLYSO_F TTCTAGAATGAGGTCTTTGCTAATCTTGG

GgLYSO_R CTACTAGTTTTTAATGAGGGATGCGTTT

Cre-F TCCAATTTACTGACCGTACACC

FSTK_UP_VF2 CACGGAACAGGTGCAAAATCGGCGTATTTTGATTACACTCCTTGCCACCTGACGTCTAAGAA

FSTK_UP_VR TACAGGCTTTTCCTGCCCGATGCTAAAACGGACAACAGTACCATTACCGCCTTTGAGTGAGC

FSTK_DOWN_VF2 TCGTATGCCGGATAAGGCGCGGTAGCGTCGCATCCGGCACTCTGCCACCTGACGTCTAAGAA

FSTK_DOWN_VR AAATTACGTCACTCCCGATCGGGAGTTCCCTCTACTCTCTATATTACCGCCTTTGAGTGAGC

Slippage_Cre-R TGGTGTACGGTCAGTAAATTGGAGACTGACTGACTGACTGACTGACTGACTGACTGACTGACTGACTCAT