Team:UNICAMP-Brazil/Notebooks/September 24

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(E. coli transformation with F plasmid)
(E. coli transformation with F plasmid)
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*''E. coli'' DH10β cells were transformed using the recircularized F plasmid, according to protocol 4.
*''E. coli'' DH10β cells were transformed using the recircularized F plasmid, according to protocol 4.
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*After the incubation time, the transformed cells were plated in LB + ampicilin culture plates, and incubated O/N at 37°C.
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*After the incubation time, the transformed cells were plated in LB + ampicilin + Xgal culture plates, and incubated O/N at 37°C.
''Gabriel''
''Gabriel''
{{:Team:UNICAMP-Brazil/inc_rodape}}
{{:Team:UNICAMP-Brazil/inc_rodape}}

Revision as of 21:59, 26 September 2009

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E. coli transformation with F plasmid

  • E. coli DH10β cells were transformed using the recircularized F plasmid, according to protocol 4.
  • After the incubation time, the transformed cells were plated in LB + ampicilin + Xgal culture plates, and incubated O/N at 37°C.

Gabriel