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Team:Paris/Transduction testing
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===Surface expression of G3P=== | ===Surface expression of G3P=== | ||
Surface expression of G3P could be seen with antibodies.<br> | Surface expression of G3P could be seen with antibodies.<br> | ||
| - | Culture of bacteria expressing OmpA-linker-G3P construction on a membrane. Adding antibodies anti-G3P. Revelation should shows whether G3P is expressed at the surface of bacteria. | + | Culture of bacteria expressing OmpA-linker-G3P construction on a membrane. Adding antibodies anti-G3P. Revelation should shows whether G3P is expressed at the surface of bacteria. |
===Floculation test=== | ===Floculation test=== | ||
Revision as of 18:58, 27 September 2009
iGEM > Paris > Reception > Testing
Tests
Surface expression of G3P
Surface expression of G3P could be seen with antibodies.
Culture of bacteria expressing OmpA-linker-G3P construction on a membrane. Adding antibodies anti-G3P. Revelation should shows whether G3P is expressed at the surface of bacteria.
Floculation test
"Fusion" could be approached by a flocculation test.
Two separate bacterial population growth on media. A population (F-) expressing the OmpA-linker_G3P construction and a population (F +) which express sexual pilus (by expression of the episome F).
