Team:UNICAMP-Brazil/Notebooks/September 13

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(→New biobrick - The first screening)
(→New biobrick - The first screening)
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* 6 colonies of each new biobrick were chosen of the plates made yesterday to grow in liquid LB.  
* 6 colonies of each new biobrick were chosen of the plates made yesterday to grow in liquid LB.  
* Then, we performed miniprep to get our new biobricks purified ([https://2009.igem.org/Team:UNICAMP-Brazil/Protocols/Mini-Prep Protocol 2]).  
* Then, we performed miniprep to get our new biobricks purified ([https://2009.igem.org/Team:UNICAMP-Brazil/Protocols/Mini-Prep Protocol 2]).  
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* We then digested the plasmids obtained by the miniprep with ''Xba''I to confirm the correct ligation of the parts following the biobrick format (Protocolo x). However, the electrophoresis gel (shown below) showed an unexpected pattern of bands that can't confirm correctly ligations of our biobricks. =(
+
* We then digested the plasmids obtained by the miniprep with ''Xba''I to confirm the correct ligation of the parts following the biobrick format ([https://2009.igem.org/Team:UNICAMP-Brazil/Protocols/T4_DNA_Ligase Protocol 11]). However, the electrophoresis gel (shown below) showed an unexpected pattern of bands that can't confirm correctly ligations of our biobricks. =(
[[Image:MInipreps e digestões Wesley.jpg |center|‎]]
[[Image:MInipreps e digestões Wesley.jpg |center|‎]]

Revision as of 05:02, 3 October 2009

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ColiGuard

finO+pSB1A3

  • After the O/N period, today we transformed the ligation finO+pSB1A3 into electrocompetent E. coli bacteria, strain DH10B. We followed protocol 3, without modifications (see Protocols section).
  • We then plated the transfomed cells in LB-AMP media, and let them grow at 37ºC for an O/N period.

finP+pSB1A3

  • We followed the same produce for the finP+pSB1A3 ligation.

Marcelo

YeastGuard

New biobrick - The first screening

  • 6 colonies of each new biobrick were chosen of the plates made yesterday to grow in liquid LB.
  • Then, we performed miniprep to get our new biobricks purified (Protocol 2).
  • We then digested the plasmids obtained by the miniprep with XbaI to confirm the correct ligation of the parts following the biobrick format (Protocol 11). However, the electrophoresis gel (shown below) showed an unexpected pattern of bands that can't confirm correctly ligations of our biobricks. =(
‎

Wesley and Gleidson




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