Team:UNICAMP-Brazil/Notebooks/September 13

(Difference between revisions)

Revision as of 22:03, 3 October 2009

After the O/N period, today we transformed the ligation finO+pSB1A3 into electrocompetent E. coli bacteria, strain DH10B. We followed Protocol 3, without modifications.
We then plated the transfomed cells in LB-AMP medium, and let them grow at 37ºC for an O/N period.

Marcelo

6 colonies of each new biobrick were chosen of the plates made yesterday to grow in liquid LB.
Then, we performed miniprep to get our new biobricks purified (Protocol 2).
We then digested the plasmids obtained by the miniprep with XbaI to confirm the correct ligation of the parts following the biobrick format (Protocol 11). However, the electrophoresis gel (shown below) showed an unexpected pattern of bands that can't confirm correctly ligations of our biobricks. =(

Wesley and Gleidson

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 ====finO+pSB1A3====
-*<p style=”text-align:justify;”>After the O/N period, today we transformed the ligation finO+pSB1A3 into electrocompetent ''E. coli'' bacteria, strain DH10B. We followed [https://2009.igem.org/Team:UNICAMP-Brazil/Protocols/Electroporation Protocol 3]
+*<p style=”text-align:justify;”>After the O/N period, today we transformed the ligation finO+pSB1A3 into electrocompetent ''E. coli'' bacteria, strain DH10B. We followed [https://2009.igem.org/Team:UNICAMP-Brazil/Protocols/Electroporation Protocol 3], without modifications.</p>
-, without modifications.</p>
 *<p style=”text-align:justify;”>We then plated the transfomed cells in LB-AMP medium, and let them grow at 37ºC for an O/N period.</p>