Team:Newcastle/IntroductoryLabwork/27 July 2009
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- | =Lab Session - | + | [[Image:Team Newcastle 2009 iGEM IntroductoryLabSessions.PNG|50px|right]] |
+ | ==Introductory Lab Session - 27th July 2009== | ||
==Introduction== | ==Introduction== | ||
- | Last week the team had completed the ethanol precipitation of the GFP and RFP (in plasmids pSB1A2 and pSB1AT3 respectively) DNA samples. They are midi-prepped plasmid DNA samples extracted using [https://2009.igem.org/Team:Newcastle/Project/Labwork/MoreProtocols#Midiprep Sigma-Aldrich's GenElute Midi-prep kit], however the ethanol present in Wash Solution 2 hadn't been completely removed so ethanol precipitation was necessary. With the ethanol removed, it should be analyse the samples through DNA gel electrophoresis. | + | Last week the team had completed the ethanol precipitation of the ''GFP'' and ''RFP'' (in plasmids ''pSB1A2'' and ''pSB1AT3'' respectively) DNA samples. They are midi-prepped plasmid DNA samples extracted using [https://2009.igem.org/Team:Newcastle/Project/Labwork/MoreProtocols#Midiprep Sigma-Aldrich's GenElute Midi-prep kit], however the ethanol present in Wash Solution 2 hadn't been completely removed so ethanol precipitation was necessary. With the ethanol removed, it should be analyse the samples through DNA gel electrophoresis. |
==Practical Outline== | ==Practical Outline== | ||
- | # Run the GFP and RFP plasmid DNA samples on gel through DNA gel electrophoresis and analyse. | + | # Run the ''GFP'' and ''RFP'' plasmid DNA samples on gel through DNA gel electrophoresis and analyse. |
==Analysing the DNA samples through DNA Gel Electrophoresis== | ==Analysing the DNA samples through DNA Gel Electrophoresis== | ||
- | [[Image:Team Newcastle 2009 iGEM Geldoc 2009-07-27 11hr 28min 1.jpg|center]] | + | [[Image:Team Newcastle 2009 iGEM Geldoc 2009-07-27 11hr 28min 1.jpg|300px|center]] |
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+ | <center> | ||
+ | {|cellpadding="10" | ||
+ | |{{#calendar: title=Team:Newcastle/IntroductoryLabwork |year=2009 | month=06 }} | ||
+ | |{{#calendar: title=Team:Newcastle/IntroductoryLabwork |year=2009 | month=07 }} | ||
+ | |} | ||
+ | </center> | ||
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{{:Team:Newcastle/Footer}} | {{:Team:Newcastle/Footer}} | ||
{{:Team:Newcastle/Right}} | {{:Team:Newcastle/Right}} |
Latest revision as of 22:30, 12 October 2009
Introductory Lab Session - 27th July 2009
Introduction
Last week the team had completed the ethanol precipitation of the GFP and RFP (in plasmids pSB1A2 and pSB1AT3 respectively) DNA samples. They are midi-prepped plasmid DNA samples extracted using Sigma-Aldrich's GenElute Midi-prep kit, however the ethanol present in Wash Solution 2 hadn't been completely removed so ethanol precipitation was necessary. With the ethanol removed, it should be analyse the samples through DNA gel electrophoresis.
Practical Outline
- Run the GFP and RFP plasmid DNA samples on gel through DNA gel electrophoresis and analyse.
Analysing the DNA samples through DNA Gel Electrophoresis
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News
Events
- 20 – 21 June 2009 - Europe workshop (London)
- 23 – 24 June 2009 - UK iGEM meetup (Edinburgh)
- 23 October Practice Presentation (Newcastle)
- 23 October T-shirts are ready
- 27 October Practice Presentation (Sunderland)
- 27 October Poster is ready
- 30 October – 2 November 2009 - Jamboree (Boston)
Social Net
- Newcastle iGEM Twitter
- [http://www.facebook.com/home.php#/group.php?gid=131709337641 Newcastle on Facebook]
- [http://www.youtube.com/user/newcastle2009igem Newcastle Youtube Channel]