Team:Edinburgh/biology(biobricks)

From 2009.igem.org

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<td><center><b>Creating Composite BioBrick</b></center></td>
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<td><center><b>tnt.R1 & tnt.R3 (Bba_xxxx)</b></center></td>
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                                 <td><center>TNT.R1 and TNT.R3 are computationally derived ligand receptors specific for TNT. The ribose-binding pocket of ribose-binding protein, a member of the E. coli periplasmic binding protein (PBP) family, was reconfigured so that each receptor recognizes TNT instead of the wild-type ligand.
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<font style="size:10px">Looger, L. L., Dwyer, M. A., Smith J. J., and Hellinga, H. W. Computational design of receptor and sensor proteins with novel functions. Nature 423, 185-190 (2003).</font> </center></td>
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Revision as of 11:06, 14 October 2009

Biology - Biobricks
Personal note

Dasha
Quick links:

tnt.R1 & tnt.R3 (Bba_xxxx)
trg-envZ (trz) fusion protein (Bba_J58104)
ompC Promoter (Bba_R0082)
Enhanced Yellow Fluorescent Protein (Bba_E0430)
onr (Bba_xxxx)
yeaR promoter (Bba_xxxx)
luxAB-gfp fusion protein (Bba_xxxx)
lump (Bba_xxxx)
luxCDE from X. luminescence (Bba_xxxx)
nsrR from Nitrosomonas Europaea (BBa_xxxx)
nirK promoter (BBa_xxxx)
Biobrick Name
Key
Description
tnt.R1 & tnt.R3 (Bba_xxxx)
TNT.R1 and TNT.R3 are computationally derived ligand receptors specific for TNT. The ribose-binding pocket of ribose-binding protein, a member of the E. coli periplasmic binding protein (PBP) family, was reconfigured so that each receptor recognizes TNT instead of the wild-type ligand.

Looger, L. L., Dwyer, M. A., Smith J. J., and Hellinga, H. W. Computational design of receptor and sensor proteins with novel functions. Nature 423, 185-190 (2003).
Edinburgh University iGEM Team 2009