Team:UNICAMP-Brazil/Notebooks/August 24

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'''RBS+CFP'''
'''RBS+CFP'''
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* After the correct RBS plasmid digestion (21/08), we ligated it to CFP previously digested with ''Xba''I and ''Pst''I according to the  [https://2009.igem.org/Team:UNICAMP-Brazil/Protocols/T4_DNA_Ligase Protocol 11].
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*<p style=”text-align:justify;”>After the correct RBS plasmid digestion (August 21), we ligated it to CFP previously digested with ''Xba''I and ''Pst''I according to [https://2009.igem.org/Team:UNICAMP-Brazil/Protocols/T4_DNA_Ligase Protocol 11].</p>
[[Image:RBSdigestion.jpg‎|500px|center]]
[[Image:RBSdigestion.jpg‎|500px|center]]
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* We also transformed ''E. coli'' according to the [https://2009.igem.org/Team:UNICAMP-Brazil/Protocols/transformation Protocol 3] with this ligation reaction.
 

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YeastGuard

Still fighting for RBS

RBS+CFP

  • After the correct RBS plasmid digestion (August 21), we ligated it to CFP previously digested with XbaI and PstI according to Protocol 11.

RBSdigestion.jpg


Raíssa





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