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Team:Paris/Addressing overview Construction
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| - | ==Construction == | + | ==Addressing the message in the membrane : Construction == |
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[[Image:Clya_construction.jpg|500px|center|Activation of our construction by Arabinose]] | [[Image:Clya_construction.jpg|500px|center|Activation of our construction by Arabinose]] | ||
| - | Here the activation of our construction by arabinose, in order to test the construction. | + | Here the activation of our construction '''by arabinose''', in order to test the construction. |
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[[Image:Clya_construction2.jpg|500px|center|Inhibition of our construction by Glucose]] | [[Image:Clya_construction2.jpg|500px|center|Inhibition of our construction by Glucose]] | ||
| - | Here the inhibition of our construction by glucose, in order to test the construction. | + | Here the inhibition of our construction '''by glucose''', in order to test the construction. |
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[[Image:ClyA_construction_3.jpg|500px|center|Activation of our construction by Glucose]] | [[Image:ClyA_construction_3.jpg|500px|center|Activation of our construction by Glucose]] | ||
| - | Here the activation of our construction by arabinose. It is quite the same as the first construction but in [ | + | Here the activation of our construction '''by arabinose'''. |
| + | <br> It is quite the same as the first construction, but in [http://www.ncbi.nlm.nih.gov/pubmed/18511069[1]] the RFP-Cter link to Nter-ClyA seems to have a better fluorescence. Because of our late about receiving the oligo for its synthesis in biobrick format, we used the first construction (ClyA-Cter link to Nter-RFP). | ||
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| + | ====Bibliography :==== | ||
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| + | [http://www.ncbi.nlm.nih.gov/pubmed/18511069[1]] Kim, J.-Y. & DeLisa, M.P. Engineered bacterial outer membrane vesicles with enhanced functionality J.Mol. Biol. (2008) 380, 51–66 | ||
Latest revision as of 00:54, 22 October 2009
iGEM > Paris > ClyA > Construction
Addressing the message in the membrane : Construction
Here the activation of our construction by arabinose, in order to test the construction.
Here the inhibition of our construction by glucose, in order to test the construction.
Here the activation of our construction by arabinose.
It is quite the same as the first construction, but in [http://www.ncbi.nlm.nih.gov/pubmed/18511069[1]] the RFP-Cter link to Nter-ClyA seems to have a better fluorescence. Because of our late about receiving the oligo for its synthesis in biobrick format, we used the first construction (ClyA-Cter link to Nter-RFP).
Bibliography :
[http://www.ncbi.nlm.nih.gov/pubmed/18511069[1]] Kim, J.-Y. & DeLisa, M.P. Engineered bacterial outer membrane vesicles with enhanced functionality J.Mol. Biol. (2008) 380, 51–66
