Team:Kyoto/GSDD/Notebook/0928-1004

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Latest revision as of 02:21, 22 October 2009

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GSDD
Notebook

0727-0802: Construction.
0803-0809: Construction.
0810-0816: Construction.
0817-0823: Construction.PCR.
0824-0830: Construction.PCR.
0831-0906: Construction.PCR.MPR.
0907-0913: Construction.PCR.MPR.
0914-0920: Construction.PCR.MPR.
0921-0927: Construction.PCR.MPR.
0928-1004: Construction.PCR.MPR.
1005-1011: Construction.PCR.MPR.
1012-1018: Construction.MPR.Observation.

0928-1004 : Construction.PCR.MPR.

Monday, 28 September

To Do

Miniprep 4(5)a, p(2)(1)2

Restriction enzyme digestion
Electorophoresis
Gel extraction

Results

Gel extraction

sample	conc./(ng/ul)
p(2)(1)E-S	6
4(5)aE-X	55

Tuesday, 29 September

To Do

4(5)

Ligation
Transformation

Wednesday, 30 September

To Do

Miniprep

Restriction enzyme digestion
Electorophoresis
Gel extraction
Ligation
Transformation

Results

Mniprep

sample	conc./(ng/ul)
2M-1	84.8
90.5

Gel extraction

Two binds were included this site, however, we couldn't and didn't have to distinguish them.

(Discussion) 2M had two kinds of plasmids, one was inserted 900bp,the other was inserted 600bp. So,2M②E-X that was extracted from agarose gel included two kinds of DNA. However, because we wanted to make only many repeats of lbinding sites of lacI, we didn’t distinguish them.

Transformation

colony were observed.

Thursday, 1 October

To Do

Colony PCR

4(5)1-7

Results

electrophoresis

2 and 3 and 5 were inserted properly.

Friday, 2 October

To Do

2Mlv2

Miniprep
Restriction Enzyme

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+==0928-1004 : Construction.PCR.MPR.==
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 ===Monday, 28 September===