Team:UNICAMP-Brazil/Notebooks/September 16

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==''' ColiGuard '''==
==''' ColiGuard '''==
====F plasmid's digestion with ''HindIII''====
====F plasmid's digestion with ''HindIII''====
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*<p style=”text-align:justify;”>The digestion with the ''HindIII'' restriction enzyme was performed according to Protocol X. In place of the second enzyme that is used in this reaction, it was used the same volume of autoclaved distilled water.</p>
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*<p style=”text-align:justify;”>The digestion with the ''Hind''III restriction enzyme was performed according to [https://2009.igem.org/Team:UNICAMP-Brazil/Protocols/Digestion_reaction Protocol 14]. In place of the second enzyme that is used in this reaction, it was used the same volume of autoclaved distilled water.</p>

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Contents

ColiGuard

F plasmid's digestion with HindIII

  • The digestion with the HindIII restriction enzyme was performed according to Protocol 14. In place of the second enzyme that is used in this reaction, it was used the same volume of autoclaved distilled water.


Gabriel

PY Promoter - Transformation

  • After the O/N period we transformed the ligations PY1 + BBa_J23100 and PY2 + BBa_J23100 into electrocompetent E. coli bacteria, strain DH10B. We followed Protocol 3 (Electroporation) without modifications.

  • After the transformation we plated the transformed cells in LB-AMP plates and let them grow at 37ºC for an O/N period.


Fabi and Léo




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