Team:Paris/NoteBook

From 2009.igem.org

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(New page: {{Template:Paris2009}} {{Template:PAris2009_menu}} == PCR products == {| |- style="background: #ccccff;" ! colspan="5" style="background: #ccccff;" | PCR products |- style="background: #c...)
 
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{{Template:Paris2009}}
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<span/ id="bottom">[https://2009.igem.org/ iGEM ] > [[Team:Paris#top | Paris]] > [[Team:Paris/NoteBook#bottom | NoteBook]]
-
{{Template:PAris2009_menu}}
+
{{Paris2009}}
 +
{{Template:Paris2009_menu}}
-
== PCR products ==
+
==NoteBook==
-
{|
+
-
|- style="background: #ccccff;"
+
-
! colspan="5" style="background: #ccccff;" | PCR products
+
-
|- style="background: #ccccff; text-align: center;"
+
-
|width=5%| Number
+
-
|width=10%| Name
+
-
|width=12.5%| Oligo F
+
-
|width=12.5%| Oligo R
+
-
|width=10%| Matrix
+
-
|width=5%| Length
+
-
|width=25%| Comments
+
-
|width=5%| Product Left ?
+
-
|width=5%| []ng/µL
+
-
|width=5%| Date
+
-
|- style="background: #cccccc;"
+
-
| style="background: #ccffcc;" |P1
+
-
|Lox71-FtsA-FtsZ-1
+
-
|O3 Lox71-FtsA-F
+
-
|O4 DEcoR1-FtsZ-R
+
-
|MG1655
+
-
|1537
+
-
|
+
-
|Ø
+
-
|
+
-
|
+
-
|- style="background: #cccccc;"
+
-
| style="background: #ccffcc;" |P2
+
-
|FtsZ-2
+
-
|O5 DEcoR1-FtsZ-F
+
-
|O2 FtsZ-R
+
-
|MG1655
+
-
|1088
+
-
|
+
-
|Ø
+
-
|
+
-
|
+
-
|- style="background: #cccccc;"
+
-
| style="background: #ccffcc;" |P3
+
-
|DGAT-1
+
-
|27 ForDGAT1
+
-
|13 DPst1-DGAT-R
+
-
|Miniprep pKS::DGAT
+
-
|1087
+
-
|
+
-
|
+
-
|
+
-
|[[Paris/July_31|July 31]]
+
-
|- style="background: #cccccc;"
+
-
| style="background: #ccffcc;" |P4
+
-
|DGAT-2
+
-
|12 DPst1-DGAT
+
-
|27 Rev-DGAT1
+
-
|Miniprep pKS::DGAT
+
-
|396
+
-
|
+
-
|
+
-
|
+
-
|[[Paris/July_31|July 31]]
+
-
|- style="background: #cccccc;"
+
-
| style="background: #ccffcc;" |P5
+
-
|Lox66-DapAColi
+
-
|O6 Lox66-DapAColi-F
+
-
|O7 DapAColi-R
+
-
|MG1655
+
-
|985
+
-
|
+
-
|Ø
+
-
|
+
-
|[[Paris/July 17| July 17]], [[Paris/July 30| July 30]]
+
-
|- style="background: #cccccc;"
+
-
| style="background: #ccffcc;" |P6
+
-
|Lox66-DapASubtilis
+
-
|O8 Lox66-DapASubt-F   
+
-
|O9 DapASubt-R
+
-
|B.Subtilis 168
+
-
|979
+
-
|
+
-
|
+
-
|
+
-
|[[Paris/July 17| July 17]],[[Paris/July 27| July 27]],[[Paris/July 30| July 30]]
+
-
|- style="background: #cccccc;"
+
-
| style="background: #ccffcc;" |P7
+
-
|Lox71-FtsZ
+
-
| O1 Lox71-FtsZ-F
+
-
| O2 FtsZ-R
+
-
|MG1655
+
-
|1260
+
-
|EcoRI site in FtsZ not mutated
+
-
|
+
-
|
+
-
|[[Paris/July 17| July 17]],[[Paris/July 30| July 30]]
+
-
|- style="background: #cccccc;"
+
-
| style="background: #ccffcc;" |P8
+
-
|RBS-DapAColi
+
-
| O20 RBS-DapAColi-F
+
-
| O7 DapAColi-R
+
-
| MG1655
+
-
|952
+
-
|B0030-DapAColi
+
-
|
+
-
|
+
-
|[[Paris/July 23| July 23]],[[Paris/July 30| July 30]]
+
-
|- style="background: #cccccc;"
+
-
| style="background: #ccffcc;" |P9
+
-
|RBS-DapASubtilis
+
-
| O21 RBS-DapASubt-F
+
-
| O9 DapASubt-R
+
-
|B.Subtilis 168
+
-
|946
+
-
|B0030-DapASubtilis
+
-
|
+
-
|
+
-
|[[Paris/July 23| July 23]], [[Paris/July 27| July 27]]
+
-
|- style="background: #cccccc;"
+
-
| style="background: #ccffcc;" |P10
+
-
|Lox71-FtsA-FtsZ
+
-
|O3 Lox71-FtsA-F
+
-
|O2 FtsZ-R
+
-
|MG1655
+
-
|2580
+
-
|EcoRI site in FtsZ not mutated
+
-
|
+
-
|
+
-
|[[Paris/July 23| July 23]]
+
-
|- style="background: #cccccc;"
+
-
| style="background: #ccffcc;" |P11
+
-
|Lox71-KmR-Lox66
+
-
| O10 Lox71-KmR-F
+
-
| O11 Lox66-KmR-R
+
-
| MP12
+
-
|1084
+
-
|
+
-
|
+
-
|
+
-
|[[Paris/July 27| July 27]],[[Paris/July 30| July 30]]
+
-
|- style="background: #cccccc;"
+
-
| style="background: #ccffcc;" |P13
+
-
|DGAT deltaPst-1
+
-
| ForDGAT1
+
-
| Megaprimer DGAT2
+
-
| DNA plasmid (pKS::DGAT):
+
-
|1443
+
-
|
+
-
|
+
-
|
+
-
|[[Paris/August_1| August 1]]
+
-
|- style="background: #cccccc;"
+
-
| style="background: #ccffcc;" |P14
+
-
|DGAT deltaPst-1 biobrick
+
-
| ForDGAT1bb
+
-
| RevDGAT1bb
+
-
| DGAT deltaPst1
+
-
|1504
+
-
|
+
-
|
+
-
|
+
-
|[[Paris/August_2| August 2]][[Paris/October 18| Oct. 18]]
+
-
|- style="background: #cccccc;"
+
-
| style="background: #ccffcc;" |P15
+
-
| PacI [pTet>>lox-GFP-loxmRFP] XmaI
+
-
| O47
+
-
| O48
+
-
| [pTet>>lox-GFP-loxmRFP] L59.6
+
-
|2kb
+
-
|
+
-
|
+
-
|
+
-
|[[Paris/August_2| August 2]]
+
-
|- style="background: #cccccc;"
+
-
| style="background: #ccffcc;" |P16
+
-
| PrefixBB-frt1-CmR-frt2-SufixBB
+
-
| O51
+
-
| O52
+
-
| pY-2C-IntC
+
-
|1kb
+
-
|
+
-
|
+
-
|
+
-
|[[Paris/October 15| Oct. 15]]
+
-
|- style="background: #cccccc;"
+
-
| style="background: #ccffcc;" |P17
+
-
| PrefixBB-frt1-CmR-frt2-T1T2-SufixBB
+
-
| O51
+
-
| O53
+
-
| pY-2C-IntC
+
-
|>1kb
+
-
|
+
-
|
+
-
|
+
-
|[[Paris/October 18| Oct. 18]]
+
-
|}
+
-
http://parts.mit.edu/r/parts/images/partbypart/icon_terminator.png
+
-
http://parts.mit.edu/images/partbypart/icon_regulatory.png
+
-
http://parts.mit.edu/r/parts/images/partbypart/icon_rbs.png
+
-
http://parts.mit.edu/r/parts/images/partbypart/icon_reporter.png
+
-
http://parts.mit.edu/registry/images/7/7b/Part_icon_cds.png
+
-
http://parts.mit.edu/registry/images/9/9f/Part_icon_dna.png
+
-
http://parts.mit.edu/registry/images/2/2e/Part_icon_generator.png
+
-
http://parts.mit.edu/registry/images/a/ab/Part_icon_plasmid.png
+
-
== Plasmids: MiniPrep Products ==
+
{| style="color:#1b2c8a;background-color:#0;" cellpadding="0" cellspacing="1" border="0" bordercolor="#fff" width="100%" align="left"
-
{|
+
!align="center"|{{ #calendar: title=Team:Paris |year=2009 | month=06 }}
-
|- style="background: #ccccff;"
+
!align="center"|{{ #calendar: title=Team:Paris |year=2009 | month=07 }}
-
! colspan="5" style="background: #ccccff;" | Plasmids: MiniPrep Products
+
!align="center"|{{ #calendar: title=Team:Paris |year=2009 | month=08 }}
-
|- style="background: #ccccff; text-align: center;"
+
!align="center"|{{ #calendar: title=Team:Paris |year=2009 | month=09 }}
-
|width=5%| Number
+
!align="center"|{{ #calendar: title=Team:Paris |year=2009 | month=10 }}
-
|width=25%| Name
+
-
|width=50%| Description
+
-
|width=15%| Products in use
+
-
|width=5%| Date
+
-
|- style="background: #cccccc;"  
+
-
| style="background: #ccffcc;" |MP1
+
-
|<bbpart>pJ23100</bbpart> in <bbpart>J61002</bbpart>
+
-
|Strong constitutive promoter in J61002 (clones 1 + 2) <partinfo>pJ23100 SpecifiedComponents</partinfo>
+
-
|
+
-
| [[Paris/July 15| July 15]], [[Paris/August 17| Aug. 17]]
+
-
|- style="background: #cccccc;"
+
-
| style="background: #ccffcc;" |MP2
+
-
|<bbpart>pJ23107</bbpart> in <bbpart>J61002</bbpart>
+
-
|Medium constitutive promoter in J61002 (clones 1 + 2) <partinfo>pJ23107 SpecifiedComponents</partinfo>
+
-
|MP2.2
+
-
| [[Paris/July 15| July 15]], [[Paris/August 17| Aug. 17]]
+
-
|- style="background: #cccccc;"  
+
-
| style="background: #ccffcc;" |MP3
+
-
|<bbpart>B0015</bbpart> in <bbpart>pSB1AK3</bbpart>
+
-
|Double terminator (clones 1 + 2) <partinfo>B0015 SpecifiedComponents</partinfo>
+
-
|MP3.3, MP3.4
+
-
| [[Paris/August 14| August 14]]
+
-
|- style="background: #cccccc;"  
+
-
| style="background: #ccffcc;" |MP4
+
-
|<bbpart>I0500</bbpart> in <bbpart>pSB2K3</bbpart>
+
-
|AraC pBAD (clones 1 + 2) <partinfo>I0500 SpecifiedComponents</partinfo>
+
-
|
+
-
| [[Paris/July 16| July 16]]
+
-
|- style="background: #cccccc;"
+
-
| style="background: #ccffcc;" |MP5
+
-
|<bbpart>BBa_B0030</bbpart> in <bbpart>pSB1A2</bbpart>
+
-
|Strong RBS (clones 1 + 2) <partinfo>B0030 SpecifiedComponents</partinfo>
+
-
|
+
-
| [[Paris/July 18| July 18]]
+
-
|- style="background: #cccccc;"
+
-
| style="background: #ccffcc;" |MP6
+
-
|<bbpart>BBa_E0422</bbpart> in <bbpart>pSB1A2</bbpart>
+
-
|ECFP (RBS+LVA+Term) (clones 1 + 2) <partinfo>E0422 SpecifiedComponents</partinfo>
+
-
|MP6'.1, MP6'.2
+
-
| [[Paris/July 18| July 18]], [[Paris/July 24| July 24]]
+
-
|- style="background: #cccccc;"
+
-
| style="background: #ccffcc;" |MP7
+
-
|<bbpart>BBa_E0241</bbpart> in <bbpart>pSB1A2</bbpart>
+
-
|PoPs to GFP converter (clones 1 + 2) <partinfo>BBa_E0241 DeepComponents</partinfo>
+
-
|MP7'.1
+
-
| [[Paris/July 18| July 18]], [[Paris/July 24| July 24]]
+
-
|- style="background: #cccccc;"  
+
-
| style="background: #ccffcc;" |MP8
+
-
|<bbpart>BBa_E0840</bbpart> in <bbpart>pSB1A2</bbpart>
+
-
|gfp tri-part; strong rbs (clones 1 + 2) <partinfo>E0840 SpecifiedComponents</partinfo>
+
-
|
+
-
| [[Paris/July 18| July 18]]
+
-
|- style="background: #cccccc;"
+
-
| style="background: #ccffcc;" |MP9
+
-
|<bbpart>BBa_J61047</bbpart> in <bbpart>pSB1A2</bbpart>
+
-
|Cre ORF (clones 1 + 2) <partinfo>J61047 SpecifiedComponents</partinfo>
+
-
|MP9'.1, MP9'.2
+
-
| [[Paris/July 18| July 18]], [[Paris/July 24| July 24]]
+
-
|- style="background: #cccccc;"
+
-
| style="background: #ccffcc;" |MP10
+
-
|<bbpart>BBa_I0500</bbpart> in <bbpart>pSB2K3</bbpart>
+
-
|AraC pBAD (clones 1 + 2) <partinfo>I0500 SpecifiedComponents</partinfo>
+
-
|
+
-
| [[Paris/July 20| July 20]]
+
-
|- style="background: #cccccc;"  
+
-
| style="background: #ccffcc;" |MP11
+
-
|<bbpart>BBa_pJ23107</bbpart> in <bbpart>p61002</bbpart>
+
-
|Medium constitutive promoter in J61002 (clones 3 + 4) <partinfo>pJ23107 SpecifiedComponents</partinfo>
+
-
|MP11'.3
+
-
| [[Paris/July 20| July 20]], [[Paris/July 23| July 23]]
+
-
|- style="background: #cccccc;"
+
-
| style="background: #ccffcc;" |MP12
+
-
|<bbpart>BBa_P1003</bbpart> in <bbpart>pSB1A2</bbpart>
+
-
|KanR <partinfo>P1003 SpecifiedComponents</partinfo>
+
-
|
+
-
| [[Paris/July 27| July 27]]
+
-
|- style="background: #cccccc;"
+
-
| style="background: #ccffcc;" |MP13
+
-
|<bbpart>BBa_J61002</bbpart>
+
-
|ptet-mRFP <partinfo>J61002 SpecifiedComponents</partinfo>
+
-
|MP13.1, MP13.2
+
-
| [[Paris/August 14| August 14]]
+
-
|- style="background: #cccccc;"  
+
-
| style="background: #ccffcc;" |MP14
+
-
|<bbpart>BBa_J61025</bbpart>
+
-
|Medium constitutive promoter in J61002 (clones 1 + 2) <partinfo>BBa_J61025 SpecifiedComponents</partinfo>
+
-
|MP14.1, MP14.2
+
-
| [[Paris/August 14| August 14]]
+
-
|- style="background: #cccccc;"
+
-
| style="background: #ccffcc;" |L1
+
-
|<bbpart>BBa_I0500</bbpart> in <bbpart>J61002</bbpart>
+
-
|construct with pBad promotor regulating expression of mRFP <partinfo>I0500 SpecifiedComponents</partinfo>
+
-
|L1".1, L1".2
+
-
| [[Paris/August 14| August 14]]
+
-
|- style="background: #cccccc;"
+
-
| style="background: #ccffcc;" |
+
-
|AttB
+
-
|
+
-
|MP15
+
-
| [[Paris/August 14| August 14]]
+
-
|- style="background: #cccccc;"  
+
-
| style="background: #ccffcc;" |
+
-
|AttP
+
-
|Finished
+
-
|MP16
+
-
| [[Paris/July 25| July 25]], [[Paris/July 27| July 27]], [[Paris/August 7| Aug 7]]
+
-
|- style="background: #cccccc;"
+
-
| style="background: #ccffcc;" |L2
+
-
|Lox66DapAE.coli in <bbpart>pSB1A2</bbpart>
+
-
|Insertion of lox66DapAE.coli construct as a biobrick in pSB1A2 plasmid
+
-
http://parts.mit.edu/registry/images/9/9f/Part_icon_dna.png
+
-
http://parts.mit.edu/registry/images/7/7b/Part_icon_cds.png
+
-
|
+
-
| [[Paris/July 25| July 25]]
+
-
|- style="background: #cccccc;"
+
-
| style="background: #ccffcc;" |L6
+
-
|<bbpart>BBa_B0030</bbpart>+<bbpart>BBa_J61047</bbpart>
+
-
|strong RBS(B0030)-Cre ORF
+
-
http://parts.mit.edu/r/parts/images/partbypart/icon_rbs.png
+
-
http://parts.mit.edu/registry/images/7/7b/Part_icon_cds.png
+
-
|L6.1, L6.2, L6.3, L6".1, L6".2
+
-
| [[Paris/July 25| July 25]], [[Paris/July 27| July 27]]
+
-
|- style="background: #cccccc;"
+
-
| style="background: #ccffcc;" |L9''
+
-
|lox66 in <bbpart>pSB1A2</bbpart>
+
-
|
+
-
|
+
-
|[[Paris/July 25| July 25]], [[Paris/July 30| July 30]]
+
-
|- style="background: #cccccc;"
+
-
| style="background: #ccffcc;" |L10''
+
-
|lox71 in <bbpart>pSB1A2</bbpart>
+
-
|
+
-
|
+
-
|[[Paris/July 25| July 25]], [[Paris/July 30| July 30]]
+
-
|- style="background: #cccccc;"
+
-
| style="background: #ccffcc;" |L12
+
-
|lox71-gfp in pSB1A2
+
-
|construction of the biobrick lox71-gfp
+
-
http://parts.mit.edu/registry/images/9/9f/Part_icon_dna.png
+
-
http://parts.mit.edu/r/parts/images/partbypart/icon_reporter.png
+
-
|L12.1 & L12.2
+
-
|[[Paris/July 30| July 30]]
+
-
|- style="background: #cccccc;"
+
-
| style="background: #ccffcc;" |L26
+
-
|araC/pbad>>RBS-Cre in <bbpart>J61002</bbpart>
+
-
|ligation ([[Paris/August 13| Aug.13]]) of RBS-Cre as a CDS behind pbad in <bbpart>J61002</bbpart>
+
-
|L26.1 (no more) & L26.2
+
-
|[[Paris/August 17| Aug. 17]]
+
-
|- style="background: #cccccc;"
+
-
| style="background: #ccffcc;" |L29
+
-
|gfptripart-lox66 <bbpart>pSB1A2</bbpart>
+
-
|ligation ([[Paris/August 13| Aug.13]]) of gfp-tripart in front of lox66
+
-
|L29.1 & L29.2
+
-
|[[Paris/August 17| Aug. 17]]
+
-
|- style="background: #cccccc;"
+
-
| style="background: #ccffcc;" |L30
+
-
|dapAp>>mRFP in <bbpart>J61002</bbpart>
+
-
|dapAp promotor placed([[Paris/August 13| Aug.13]]) upstream of mRFP <bbpart>J61002</bbpart>
+
-
|L30A1 & L30A2
+
-
|[[Paris/August 17| Aug. 17]]
+
-
|- style="background: #cccccc;"
+
-
| style="background: #ccffcc;" |L31
+
-
|lox71-<bbpart>B0015</bbpart>(T) in <bbpart>pSB1A2</bbpart>
+
-
|
+
-
|L31.1 & L31.3
+
-
|[[Paris/August 20| Aug. 20]]
+
-
|- style="background: #cccccc;"
+
-
| style="background: #ccffcc;" |L32
+
-
|<bbpart>B0015</bbpart>(T)-lox66 in <bbpart>pSB1A2</bbpart>
+
-
|
+
-
|L32.1 & L32.2
+
-
|[[Paris/August 20| Aug. 20]]
+
-
|- style="background: #cccccc;"
+
-
| style="background: #ccffcc;" |L34
+
-
|pTet>>lox71-gfptripart in <bbpart>pSB1A2</bbpart>
+
-
|
+
-
|L34.1 & L34.2
+
-
|[[Paris/August 20| Aug. 20]]
+
-
|- style="background: #cccccc;"
+
-
| style="background: #ccffcc;" |L35
+
-
|araC/pBad>>lox71-gfptripart in <bbpart>pSB1A2</bbpart>
+
-
|il manque la seq. de 740-1410
+
-
|L35.1 & L35.2
+
-
|[[Paris/August 20| Aug. 20]]
+
-
|- style="background: #cccccc;"
+
-
| style="background: #ccffcc;" |L36
+
-
|lox66-mRFP
+
-
|
+
-
|L36.1 & L36.2
+
-
|[[Paris/August 20| Aug. 20]]
+
-
|- style="background: #cccccc;"
+
-
| style="background: #ccffcc;" |L37
+
-
|lox66-RBS-dapAcoli
+
-
|
+
-
|L37.1 & L37.2
+
-
|[[Paris/August 20| Aug. 20]]
+
-
|- style="background: #cccccc;"
+
-
| style="background: #ccffcc;" |L39
+
-
|RBS-dapAcoli cloned in <bbpart>pSB1A2</bbpart>
+
-
|
+
-
|L39.1 & L39.2
+
-
|[[Paris/August 20| Aug. 20]]
+
-
|- style="background: #cccccc;"
+
-
| style="background: #ccffcc;" |L46
+
-
|pTet>>lox71-B0015(T) in <bbpart>pSB1A2</bbpart>
+
-
|
+
-
|L46.1
+
-
|[[Paris/September 18| Sep. 18]]
+
-
|- style="background: #cccccc;"
+
-
| style="background: #ccffcc;" |L53
+
-
|B0015(T)-lox66-RBSDapAcoli in <bbpart>pSB1A2</bbpart>
+
-
|
+
-
|L53.7
+
-
|[[Paris/September 18| Sep. 18]]
+
-
|- style="background: #cccccc;"
+
-
| style="background: #ccffcc;" |L56
+
-
|B0015(T)-lox66-RBSdapAsubtilis in <bbpart>pSB1A2</bbpart>
+
-
|
+
-
|L56.3 (attention, seq a partiellement foiré, refaire seq)
+
-
|[[Paris/September 18| Sep. 18]]
+
-
|- style="background: #cccccc;"
+
-
| style="background: #ccffcc;" |L59
+
-
|pTet>>lox71-gfptripart-lox66-mRFP <bbpart>pSB1A2</bbpart>
+
-
|
+
-
|L59.6 (attention, seq avec O40 a foiré, mais les extrémités sont bonnes, je pense que c ok car les colonies sont vertes)
+
-
|[[Paris/September 18| Sep. 18]]
+
-
|- style="background: #cccccc;"
+
-
| style="background: #ccffcc;" |L64
+
-
|RBSdapAsubtilis cloned in <bbpart>pSB1A2</bbpart>
+
-
|
+
-
|L564.1 & L64.2
+
-
|[[Paris/September 18| Sep. 18]]
+
-
|- style="background: #cccccc;"
+
-
| style="background: #ccffcc;" |MPT1
+
-
|Lox66-DapAColi
+
-
|pTOPO P5
+
-
|MPT1.1, MPT1.2
+
-
|[[Paris/August 7| Aug 7]]
+
-
|- style="background: #cccccc;"
+
-
| style="background: #ccffcc;" |MPT3
+
-
|Lox66-DapASubtilis
+
-
|pTOPO P6
+
-
|MPT3.2, MPT3.1
+
-
|[[Paris/August 7| Aug 7]]
+
-
|- style="background: #cccccc;"
+
-
| style="background: #ccffcc;" |MPT4
+
-
|RBS-DapAColi
+
-
|pTOPO P8
+
-
|MPT4.1, .2, .3, .4& .5
+
-
|[[Paris/August 7| Aug 7]]
+
-
|- style="background: #cccccc;"
+
-
| style="background: #ccffcc;" |MPT5
+
-
|RBS-DapASubtilis
+
-
|pTOPO P9
+
-
|MPT5.1, .2 &.3
+
-
|[[Paris/August 7| Aug 7]]
+
-
|- style="background: #cccccc;"
+
-
| style="background: #ccffcc;" |MP15
+
-
|pKD46
+
-
|
+
-
|
+
-
|[[Paris/October 2| Oct 2]]
+
|}
|}
-
== Digestion Products ==
 
-
<br>
 
-
<font color="#FF99FF" size="4">PURPLE = Vector </font><br>
 
-
<font color="#ccffcc" size="4">GREEN = Insert </font><br>
 
-
{|
 
-
|- style="background: #ccccff;"
 
-
! colspan="5" style="background: #ccccff;" | Digestion Products
 
-
|- style="background: #ccccff; text-align: center;"
 
-
|width=5%| Number
 
-
|width=20%| Product Name
 
-
|width=20%| Matrix Name
 
-
|width=5%| Enzyme 1
 
-
|width=5%| Enzyme 2
 
-
|width=5%| Size
 
-
|width=30%| Description
 
-
|width=5%| Product Left
 
-
|width=5%| []ng/µL
 
-
|width=5%| Date
 
-
|- style="background: #cccccc;"
 
-
| style="background: #FF99FF;" |D1
 
-
|<bbpart>pJ23100</bbpart>
 
-
|<bbpart>pJ23100</bbpart>
 
-
|Spe1
 
-
|Pst1
 
-
|2096
 
-
|Vector with the J23100 promoter ready for insertion of a RBS/CDS insert
 
-
|Ø (24/09/07)
 
-
|34
 
-
|[[Paris/July 17| July 17]]
 
-
|- style="background: #cccccc;"
 
-
| style="background: #ccffcc;" |D2
 
-
|<bbpart>mRFP dblTer</bbpart>
 
-
|<bbpart>pJ23100</bbpart>
 
-
|Spe1
 
-
|Pst1
 
-
|887
 
-
|
 
-
|
 
-
|27
 
-
|[[Paris/July 17| July 17]]
 
-
|- style="background: #cccccc;"
 
-
| style="background: #FF99FF;" |D3
 
-
|<bbpart>pJ23107</bbpart>
 
-
|<bbpart>pJ23107</bbpart>
 
-
|Spe1
 
-
|Pst1
 
-
|2096
 
-
|Vector with the J23107 promoter ready for insertion of a RBS/CDS insert
 
-
|Ø (24/09/07)
 
-
|32
 
-
|[[Paris/July 17| July 17]]
 
-
|- style="background: #cccccc;"
 
-
| style="background: #FF99FF;" |D4
 
-
|<bbpart>mRFP dblTer</bbpart>
 
-
|<bbpart>pJ23107</bbpart>
 
-
|Spe1
 
-
|Pst1
 
-
|887
 
-
|
 
-
|
 
-
|25
 
-
|[[Paris/July 17| July 17]]
 
-
|- style="background: #cccccc;"
 
-
| style="background: #FF99FF;" |D5
 
-
|<bbpart>pJ23100</bbpart>
 
-
|<bbpart>pJ23100</bbpart>
 
-
|EcoR1
 
-
|Spe1
 
-
|2925
 
-
|The vector with the mRFP gene ready for insertion of a promoter. We are not sure the digestion worked properly.
 
-
|
 
-
|28&29
 
-
|[[Paris/July 17| July 17]]
 
-
|- style="background: #cccccc;"
 
-
| style="background: #FF99FF;" |D6
 
-
|<bbpart>pJ23107</bbpart>
 
-
|<bbpart>pJ23107</bbpart>
 
-
|EcoR1
 
-
|Spe1
 
-
|2925
 
-
|The vector with the mRFP gene ready for insertion of a promoter. We are not sure the digestion worked properly.
 
-
|
 
-
|31&27
 
-
|[[Paris/July 17| July 17]]
 
-
|- style="background: #cccccc;"
 
-
| style="background: #ccffcc;" |D7
 
-
|Lox66 DapA E.coli
 
-
|Lox66 DapA E.coli (PCR product)
 
-
|EcoR1
 
-
|Pst1
 
-
|977
 
-
|
 
-
|
 
-
|24
 
-
|[[Paris/July 17| July 17]]
 
-
|- style="background: #cccccc;"
 
-
| style="background: #ccffcc;" |D8
 
-
|Lox66 DapA E.coli
 
-
|Lox66 DapA E.coli (PCR product)
 
-
|Xba1
 
-
|Pst1
 
-
|962
 
-
|
 
-
|
 
-
|44
 
-
|[[Paris/July 17| July 17]]
 
-
|- style="background: #cccccc;"
 
-
| style="background: #FF99FF;" |D9
 
-
|<bbpart>pJ23100</bbpart> ES vector
 
-
|<bbpart>pJ23100</bbpart> (MP1.1 & MP1.2)
 
-
|EcoI
 
-
|SpeI
 
-
|2925
 
-
|Vector with mRFP RBS/CDS ready for insertion promoter (fw-insert)
 
-
|
 
-
|80&43
 
-
|[[Paris/July 21| July 21]]
 
-
|- style="background: #cccccc;"
 
-
| style="background: #ccffcc;" |D10
 
-
|<bbpart>pJ23100</bbpart> promoter fw-insert
 
-
|<bbpart>pJ23100</bbpart> (MP1.1 & MP1.2)
 
-
|EcoI
 
-
|SpeI
 
-
|58
 
-
|J23100 promoter ready for use as a fw-insert
 
-
|
 
-
|35&28
 
-
|[[Paris/July 21| July 21]]
 
-
|- style="background: #cccccc;"
 
-
| style="background: #FF99FF;" |D11
 
-
|<bbpart>pJ23107</bbpart> ES vector
 
-
|<bbpart>pJ23107</bbpart> (MP2.1, MP2.2, MP2.3 & MP2.4)
 
-
|EcoRI
 
-
|SpeI
 
-
|2925
 
-
|Vector with mRFP RBS/CDS ready for insertion of a fw-insert promoter
 
-
|
 
-
|49,35,28&41
 
-
|[[Paris/July 21| July 21]]
 
-
|- style="background: #cccccc;"
 
-
| style="background: #ccffcc;" |D12
 
-
|<bbpart>pJ23107</bbpart> promoter fw-insert
 
-
|<bbpart>pJ23107</bbpart> (MP2.1, MP2.2, MP2.3 & MP2.4)
 
-
|EcoRI
 
-
|SpeI
 
-
|58
 
-
|J23107 promoter ready for usage as a fw-insert
 
-
|
 
-
|23,25,32&32
 
-
|[[Paris/July 21| July 21]]
 
-
|- style="background: #cccccc;"
 
-
| style="background: #ccffcc;" |D13
 
-
|<bbpart>mRFP</bbpart>
 
-
|<bbpart>pJ23107</bbpart> (MP2.2, MP2.3 & MP2.4)
 
-
|SpeI
 
-
|PstI
 
-
|887
 
-
|mRFP RBS/CDS/T sequence, can it be used as a backward insert? => yes but you will not be able to add any biobrick after it
 
-
|
 
-
|21&41
 
-
|[[Paris/July 21| July 21]]
 
-
|- style="background: #cccccc;"
 
-
| style="background: #ccffcc;" |D14
 
-
|Cre ORF
 
-
|<bbpart>J61047</bbpart> MP9.1 & MP9.2
 
-
|XbaI
 
-
|PstI
 
-
|1063
 
-
|Cre ORF extracted for usage as a bw-insert
 
-
|Ø D14.1
 
-
|38&41
 
-
|[[Paris/July 21| July 21]]
 
-
|- style="background: #cccccc;"
 
-
| style="background: #FF99FF;" |D15
 
-
|<bbpart>B0030</bbpart> bw-vector
 
-
|<bbpart>B0030</bbpart> MP5.1 & MP5.2
 
-
|SpeI
 
-
|PstI
 
-
|2076
 
-
|bacward vector for cloning a CDS bw-insert after the B0030 RBS
 
-
|
 
-
|87&42
 
-
|[[Paris/July 21| July 21]]
 
-
|- style="background: #cccccc;"
 
-
| style="background: #ccffcc;" |D18
 
-
|BB dig. lox66DapAE.coli
 
-
|lox66-DapAE.coli P5 PCR product
 
-
|EcoRI
 
-
|PstI
 
-
|~960
 
-
|PCR product digested for cloning as a BioBrick
 
-
|Ø
 
-
|44
 
-
|[[Paris/July 21| July 21]]
 
-
|- style="background: #cccccc;"
 
-
| style="background: #ccffcc;" |D19
 
-
|BB dig. lox66DapAsubtilis
 
-
|lox66-DapAsubtilis P6 PCR product
 
-
|EcoRI
 
-
|PstI
 
-
|~960
 
-
|PCR product digested for cloning as a BioBrick
 
-
|Ø
 
-
|
 
-
|[[Paris/July 21| July 21]]
 
-
|- style="background: #cccccc;"
 
-
| style="background: #ccffcc;" |D19'
 
-
|BB dig. lox66DapAsubtilis
 
-
|lox66-DapAsubtilis P6' PCR product
 
-
|EcoRI
 
-
|PstI
 
-
|~960
 
-
|PCR product digested for cloning as a BioBrick
 
-
|
 
-
|16
 
-
|[[Paris/July 30| July 30]]
 
-
|- style="background: #cccccc;"
 
-
| style="background: #ccffcc;" |D20
 
-
|bw-insert lox66-DapAE.coli
 
-
|lox66-DapAE.coli P5 PCR product
 
-
|XbaI
 
-
|PstI
 
-
|~960
 
-
|PCR product digested for usage as a backward insert
 
-
|Ø
 
-
|
 
-
|[[Paris/July 21| July 21]]
 
-
|- style="background: #cccccc;"
 
-
| style="background: #ccffcc;" |D21
 
-
|bw-insert lox66-DapAsubtilis
 
-
|lox66-DapAsubtilis P6 PCR product
 
-
|XbaI
 
-
|PstI
 
-
|~960
 
-
|PCR product digested for usage as a backward insert
 
-
|Ø
 
-
|
 
-
|[[Paris/July 21| July 21]]
 
-
|- style="background: #cccccc;"
 
-
| style="background: #ccffcc;" |D21'
 
-
|bw-insert lox66-DapAsubtilis
 
-
|lox66-DapAsubtilis P6' PCR product
 
-
|XbaI
 
-
|PstI
 
-
|~960
 
-
|PCR product digested for cloning as a BI
 
-
|
 
-
|34
 
-
|[[Paris/July 30| July 30]]
 
-
|- style="background: #cccccc;"
 
-
| style="background: #FF99FF;" |D22
 
-
|pSB1A2 open vector
 
-
|<bbpart>BBa_J61047</bbpart> (MP9.1 & MP9.2)
 
-
|EcoI
 
-
|PstI
 
-
|Ø D22.1
 
-
|Open pSB1A2 vector for BioBrick EcoRI/PstI insertio
 
-
|Ø (24/09/07)
 
-
|D22.2:59
 
-
|[[Paris/July 23| July 23]],[[Paris/August 13 | Aug. 13 ]]
 
-
|- style="background: #cccccc;"
 
-
| style="background: #ccffcc;" |D23
 
-
|AraC/pBad promoter fw-insert
 
-
|<bbpart>BBa_I0500</bbpart> (MP4.1 & MP4.2)
 
-
|EcoI
 
-
|SpeI
 
-
|1233
 
-
|AraC/pBad promoter ready for use as a forward insert
 
-
|Ø D23.1
 
-
|93&53
 
-
|[[Paris/July 23| July 23]]
 
-
|- style="background: #cccccc;"
 
-
| style="background: #ccffcc;" |D24
 
-
|eCFP bw-insert
 
-
|<bbpart>BBa_E0422</bbpart> (MP6.1 & MP6.2)
 
-
|XbaI
 
-
|PstI
 
-
|
 
-
|eCFP (RBS-OFR_LVA-T) ready for use as a backward insert
 
-
|
 
-
|56&55
 
-
|[[Paris/July 23| July 23]]
 
-
|- style="background: #cccccc;"
 
-
| style="background: #ccffcc;" |D26
 
-
|gfp-tripart
 
-
|<bbpart>BBa_E0840</bbpart> (MP8.1 & MP8.2)
 
-
|XbaI
 
-
|PstI
 
-
|
 
-
|gfp-tri part (strongRBS-ORF-T) ready for use as a backward insert
 
-
|
 
-
|47&47
 
-
|[[Paris/July 23| July 23]]
 
-
|- style="background: #cccccc;"
 
-
| style="background: #ccffcc;" |D27
 
-
|lox71-ftsZ bw-insert
 
-
|lox71-ftsZ PCR product P7
 
-
|XbaI
 
-
|PstI
 
-
|
 
-
|lox71-ftsZ(unmutated) ready for use as a backward insert
 
-
|Ø
 
-
|
 
-
|[[Paris/July 23| July 23]]
 
-
|- style="background: #cccccc;"
 
-
| style="background: #ccffcc;" |D27'
 
-
|lox71-ftsZ bw-insert
 
-
|lox71-ftsZ PCR product P7
 
-
|XbaI
 
-
|PstI
 
-
|
 
-
|lox71-ftsZ(unmutated) ready for use as a backward insert
 
-
|
 
-
|24
 
-
|[[Paris/July 30| July 30]]
 
-
|- style="background: #cccccc;"
 
-
| style="background: #ccffcc;" |D28
 
-
|RBS-DapAColi BB
 
-
|P8 (RBS-DapAColi)
 
-
|EcoRI
 
-
|PstI
 
-
|~950
 
-
|Biobrick
 
-
|
 
-
|54
 
-
|[[Paris/July 25| July 25]]
 
-
|- style="background: #cccccc;"
 
-
| style="background: #ccffcc;" |D29
 
-
|RBS-DapAColi
 
-
|P8 (RBS-DapAColi)
 
-
|XbaI
 
-
|PstI
 
-
|~950
 
-
|Backward insert
 
-
|
 
-
|44
 
-
|[[Paris/July 25| July 25]]
 
-
|- style="background: #cccccc;"
 
-
| style="background: #ccffcc;" |D30
 
-
|RBS-DapASubtilis BB
 
-
|P9 (RBS-DapASubtilis)
 
-
|EcoRI
 
-
|PstI
 
-
|~950
 
-
|Biobrick
 
-
|
 
-
|72
 
-
|[[Paris/July 25| July 25]]
 
-
|- style="background: #cccccc;"
 
-
| style="background: #ccffcc;" |D31
 
-
|RBS-DapASubtilis
 
-
|P9 (RBS-DapASubtilis)
 
-
|XbaI
 
-
|PstI
 
-
|~950
 
-
|Backward insert
 
-
|
 
-
|51
 
-
|[[Paris/July 25| July 25]]
 
-
|- style="background: #cccccc;"
 
-
| style="background: #ccffcc;" |D32
 
-
|Lox71-FtsA-FtsZ
 
-
|P10 Lox71-FtsA-FtsZ
 
-
|XbaI
 
-
|PstI
 
-
|~2600
 
-
|Backward insert - EcoRI site still presents : we can't use EcoRI
 
-
|
 
-
|47
 
-
|[[Paris/July 25| July 25]]
 
-
|- style="background: #cccccc;"
 
-
| style="background: #ccffcc;" |D33
 
-
|RBS(B0030)CreORF
 
-
|L6' (clone 1 & 2)
 
-
|XbaI
 
-
|PstI
 
-
|
 
-
|RBS-Cre digestion for use as a BI
 
-
|
 
-
|29&27
 
-
|[[Paris/July 30| July 30]],[[Paris/August 13| August 13]]
 
-
|- style="background: #cccccc;"
 
-
| style="background: #ccffcc;" |D34
 
-
|lox66
 
-
|L9' lox66
 
-
|SpeI
 
-
|PstI
 
-
|
 
-
|Biobrick plasmid carrying lox66 digested as a BV
 
-
|
 
-
|18
 
-
|[[Paris/July 30| July 30]]
 
-
|- style="background: #cccccc;"
 
-
| style="background: #ccffcc;" |D35
 
-
|lox71
 
-
|L10' lox71
 
-
|SpeI
 
-
|PstI
 
-
|
 
-
|Biobrick plasmid carrying lox71 digested as a BV
 
-
|
 
-
|
 
-
|[[Paris/July 30| July 30]],[[Paris/August 13| August 13]]
 
-
|- style="background: #cccccc;"
 
-
| style="background: #ccffcc;" |D36
 
-
|lox71-KmR-lox66
 
-
|P11 lox71-KmR-lox66
 
-
|EcoRI
 
-
|PstI
 
-
|
 
-
|lox71-KmR-lox66 digested as a biobrick insert
 
-
|
 
-
|
 
-
|[[Paris/July 30| July 30]]
 
-
|- style="background: #cccccc;"
 
-
| style="background: #ccffcc;" |D37
 
-
|lox71-gfp(tripart) BI
 
-
|L12.1 & L12.2 miniprep products (from [[Paris/July 30| 30/07]] ligation reaction)
 
-
|XbaI
 
-
|PstI
 
-
|
 
-
|lox71_RBS-gfp-double terminator extracted as a backward insert
 
-
|
 
-
|
 
-
|[[Paris/August 6 | Aug. 6 ]]
 
-
|- style="background: #cccccc;"
 
-
| style="background: #ccffcc;" |D38
 
-
|BB-insert lox66-DapAE.coli
 
-
|lox66-DapAE.coli in pTOPO (P5 PCR product)(clones 1+2)
 
-
|EcoRI
 
-
|PstI
 
-
|
 
-
|lox66-dapAE.coli extracted from pTOPO plasmid as a BB insert
 
-
|
 
-
|
 
-
|[[Paris/August 6| Aug. 6]]
 
-
|- style="background: #cccccc;"
 
-
| style="background: #ccffcc;" |D39'
 
-
|BI-insert lox66-DapAE.coli
 
-
|lox66-DapAE.coli in pTOPO (P5 PCR product)(clones 1+2)
 
-
|XbaI
 
-
|PstI
 
-
|
 
-
|lox66-dapAcoli extracted for usage as a backward insert
 
-
|
 
-
|
 
-
|[[Paris/August 7 | Aug. 7 ]]
 
-
|- style="background: #cccccc;"
 
-
| style="background: #ccffcc;" |D40
 
-
|BB-insert lox71-KmR-lox66
 
-
|L25.1 miniprep product (from ligation rxn of [[Paris/July 30| 30/07]]
 
-
|EcoRI
 
-
|PstI
 
-
|
 
-
|lox71-KmR-lox66 construct extracted for clonong as a biobrick
 
-
|
 
-
|
 
-
|[[Paris/August 6 | Aug. 6 ]]
 
-
|- style="background: #cccccc;"
 
-
| style="background: #FF99FF;" |D41
 
-
|araC/pBad-J61002 plasmid SP dig.
 
-
|L1.2, L1".1 & L1".2 miniprep product
 
-
|SpeI
 
-
|PstI
 
-
|
 
-
|J61002 plasmid containing araC/pBad promotor digested for insertion of a BI CDS
 
-
|
 
-
|
 
-
|[[Paris/August 13 | Aug. 13 ]]
 
-
|- style="background: #cccccc;"
 
-
| style="background: #FF99FF;" |D42
 
-
|Lox66 FV
 
-
|L9
 
-
|EcoRI
 
-
|XbaI
 
-
|
 
-
|pSB1A2-lox66 as a Forward vector
 
-
|
 
-
|
 
-
|[[Paris/August 13| August 13]]
 
-
|- style="background: #cccccc;"
 
-
| style="background: #ccffcc;" |D43
 
-
|gfp-tripart FI
 
-
|MP8.1
 
-
|EcoRI
 
-
|SpeI
 
-
|
 
-
|gfp-tri part (strongRBS-ORF-T) ready for use as a forward insert
 
-
|
 
-
|
 
-
|[[Paris/August 13| August 13]]
 
-
|- style="background: #cccccc;"
 
-
| style="background: #ccffcc;" |D44
 
-
|<bbpart>B0015</bbpart> terminator BI
 
-
|<bbpart>B0015</bbpart> in <bbpart>pSB1AK3</bbpart> (MP3.3 Aug.14)
 
-
|XbaI
 
-
|Pst1
 
-
|
 
-
|terminator B0015 extracted for use as a BI
 
-
|
 
-
|
 
-
|[[Paris/August 16| August 16]]
 
-
|- style="background: #cccccc;"
 
-
| style="background: #ccffcc;" |D45
 
-
|<bbpart>B0015</bbpart> terminator FI
 
-
|<bbpart>B0015</bbpart> in <bbpart>pSB1AK3</bbpart> (MP3.4 Aug.14)
 
-
|EcoRI
 
-
|SpeI
 
-
|
 
-
|terminator B0015 extracted for use as a FI
 
-
|
 
-
|
 
-
|[[Paris/August 16| August 16]]
 
-
|- style="background: #cccccc;"
 
-
| style="background: #FF99FF;" |D46
 
-
|<bbpart>B0015</bbpart> BV
 
-
|<bbpart>B0015</bbpart> in <bbpart>pSB1AK3</bbpart> (MP3.3 Aug.14)
 
-
|SpeI
 
-
|Pst1
 
-
|
 
-
|<bbpart>B0015</bbpart> in <bbpart>pSB1AK3</bbpart> plasmid extracted for use as a BV
 
-
|
 
-
|
 
-
|[[Paris/August 16| August 16]]
 
-
|- style="background: #cccccc;"
 
-
| style="background: #FF99FF;" |D47
 
-
|<bbpart>pSB1A2</bbpart> XP dig.
 
-
|<bbpart>pSB1A2</bbpart> carrying Cre ORF (MP9.1)
 
-
|XbaI
 
-
|PstI
 
-
|
 
-
|<bbpart>pSB1A2</bbpart> open vector ready for cloning of BI
 
-
|
 
-
|
 
-
|[[Paris/August 16| August 16]]
 
-
|- style="background: #cccccc;"
 
-
| style="background: #FF99FF;" |D48
 
-
|<bbpart>pSB1A2</bbpart> ES dig.
 
-
|<bbpart>pSB1A2</bbpart> carrying Cre ORF (MP9.2)
 
-
|EcoRI
 
-
|SpeI
 
-
|
 
-
|<bbpart>pSB1A2</bbpart> open vector ready for cloning of FI
 
-
|
 
-
|
 
-
|[[Paris/August 16| August 16]]
 
-
|- style="background: #cccccc;"
 
-
| style="background: #ccffcc;" |D49
 
-
|lox71-gfptripart BI
 
-
|lox71-gfptripart in <bbpart>pSB1A2</bbpart> (L12.2)
 
-
|XbaI
 
-
|Pst1
 
-
|
 
-
|lox71-gfptripart extracted for use as a BI
 
-
|
 
-
|
 
-
|[[Paris/August 16| August 16]]
 
-
|- style="background: #cccccc;"
 
-
| style="background: #FF99FF;" |D50
 
-
|<bbpart>BBa_J61002</bbpart> pTet SP dig.
 
-
|<bbpart>BBa_J61002</bbpart> (MP13.1 [[Paris/August 14| Aug. 14]])
 
-
|SpeI
 
-
|PstI
 
-
|
 
-
|<bbpart>BBa_J61002</bbpart> pTet promoter vector ready for insertion of CDS as a BI
 
-
|
 
-
|
 
-
|[[Paris/August 16| August 16]]
 
-
|- style="background: #cccccc;"
 
-
| style="background: #ccffcc;" |D51
 
-
|RBS-dapAcoli BBinsert
 
-
|pTOPO of RBS-dapAcoli (MPT4.3)
 
-
|EcoRI
 
-
|Pst1
 
-
|
 
-
|RBS-dapAcoli extracted for cloning as a biobrick
 
-
|
 
-
|
 
-
|[[Paris/August 16| August 16]]
 
-
|- style="background: #cccccc;"
 
-
| style="background: #ccffcc;" |D52
 
-
|RBS-dapAcoli BI
 
-
|pTOPO of RBS-dapAcoli (MPT4.3)
 
-
|XbaI
 
-
|Pst1
 
-
|
 
-
|RBS-dapAcoli extracted for use as a BI
 
-
|
 
-
|
 
-
|[[Paris/August 16| August 16]]
 
-
|- style="background: #cccccc;"
 
-
| style="background: #ccffcc;" |D53
 
-
|RBS-dapAsubtilis BBinsert
 
-
|pTOPO of RBS-dapAsubtilis (MPT5.1)
 
-
|EcoRI
 
-
|Pst1
 
-
|
 
-
|RBS-dapAsubtilis extracted for cloning as a biobrick
 
-
|
 
-
|
 
-
|[[Paris/August 16| August 16]]
 
-
|- style="background: #cccccc;"
 
-
| style="background: #ccffcc;" |D54
 
-
|RBS-dapAsubtilis BI
 
-
|pTOPO of RBS-dapAsubtilis (MPT5.1)
 
-
|XbaI
 
-
|Pst1
 
-
|
 
-
|RBS-dapAsubtilis extracted for use as a BI
 
-
|Ø (24/09/07)
 
-
|
 
-
|[[Paris/August 16| August 16]]
 
-
|- style="background: #cccccc;"
 
-
| style="background: #ccffcc;" |D55
 
-
|RBS-dapAsubtilis FI
 
-
|pTOPO of RBS-dapAsubtilis (MPT5.2)
 
-
|EcoRI
 
-
|SpeI
 
-
|
 
-
|RBS-dapAsubtilis extracted for use as a FI
 
-
|
 
-
|
 
-
|[[Paris/August 16| August 16]]
 
-
|- style="background: #cccccc;"
 
-
| style="background: #ccffcc;" |D57
 
-
|lox71-B0015(T) BI
 
-
|(L31.1, L31.3) lox71-B0015(T) <bbpart>pSB1A2</bbpart>
 
-
|XbaI
 
-
|Pst1
 
-
|
 
-
|lox71-B0015(T) extracted for use as a BI <bbpart>pSB1A2</bbpart>
 
-
|
 
-
|
 
-
|[[Paris/September 14| Sep. 14]]
 
-
|- style="background: #cccccc;"
 
-
| style="background: #FF99FF;" |D58
 
-
|B0015(T)-lox66 BV
 
-
|(L32.1, L32.2) B0015(T)-lox66 BV
 
-
|SpeI
 
-
|PstI
 
-
|
 
-
|B0015(T)-lox66 for use as a BV <bbpart>pSB1A2</bbpart>
 
-
|
 
-
|
 
-
|[[Paris/September 14| Sep. 14]]
 
-
|- style="background: #cccccc;"
 
-
| style="background: #ccffcc;" |D59
 
-
|pTet>>lox71-gfptripart BI
 
-
|(L34.1, L34.2) pTet>>lox71-gfptripart <bbpart>BBa_J61002</bbpart>
 
-
|XbaI
 
-
|PstI
 
-
|
 
-
|pTet>>lox71-gfptripart for use as a BI
 
-
|
 
-
|
 
-
|[[Paris/September 14| Sep. 14]]
 
-
|- style="background: #cccccc;"
 
-
| style="background: #FF99FF;"  |D61
 
-
|lox66-mRFP FV
 
-
|(L36.1, L36.2) lox66-mRFP
 
-
|EcoRI
 
-
|XbaI
 
-
|
 
-
|lox66-mRFP part (strongRBS-ORF-T) ready for use as a forward vector
 
-
|
 
-
|
 
-
|[[Paris/September 14| Sep. 14]]
 
-
|- style="background: #cccccc;"
 
-
| style="background: #ccffcc;" |D62
 
-
|lox66-RBSDapAcoli BI
 
-
|(L37.1, L37.2) lox66-RBSDapAcoli
 
-
|XbaI
 
-
|PstI
 
-
|
 
-
|lox66-RBSDapAcoli ready for use as a Backward insert
 
-
|
 
-
|
 
-
|[[Paris/September 14| Sep. 14]]
 
-
|- style="background: #cccccc;"
 
-
| style="background: #ccffcc;" |D63
 
-
|RBSDapAcoli BI
 
-
|(L39.1, L39.2) RBSDapAcoli
 
-
|XbaI
 
-
|PstI
 
-
|
 
-
|RBSDapAcoli BI (for insertion behind T-lox66)
 
-
|
 
-
|
 
-
|[[Paris/September 14| Sep. 14]]
 
-
|- style="background: #cccccc;"
 
-
| style="background: #FF99FF;" |D64
 
-
|FRT-CmR-FRT <bbpart>BBa_J61025</bbpart> BV
 
-
|(MP14.1) <bbpart>BBa_J61025</bbpart>
 
-
|SpeI
 
-
|PstI
 
-
|
 
-
|FRT-CmR-FRT <bbpart>BBa_J61025</bbpart> (for backward introduction of a casette for Wanner K insertion)
 
-
|
 
-
|
 
-
|[[Paris/September 14| Sep. 14]]
 
-
|- style="background: #cccccc;"
 
-
| style="background: #FF99FF;" |D65
 
-
|FRT-CmR-FRT <bbpart>BBa_J61025</bbpart> FV
 
-
|(MP14.1) <bbpart>BBa_J61025</bbpart>
 
-
|EcoR
 
-
|XbaI
 
-
|
 
-
|FRT-CmR-FRT <bbpart>BBa_J61025</bbpart> (for forward introduction of a casette for Wanner K insertion)
 
-
|
 
-
|
 
-
|[[Paris/September 14| Sep. 14]]
 
-
|- style="background: #cccccc;"
 
-
| style="background: #FF99FF;" |D68
 
-
|attB BV
 
-
|(MP15.1)
 
-
|SpeI
 
-
|PstI
 
-
|
 
-
|vector containing attB for use as a BV
 
-
|
 
-
|
 
-
|[[Paris/September 14| Sep. 14]]
 
-
|- style="background: #cccccc;"
 
-
| style="background: #FF99FF;" |D69
 
-
|gfptripart-lox66 BV
 
-
|(L29.1) gfptripart-lox66 <bbpart>pSB1A2</bbpart>
 
-
|SpeI
 
-
|PstI
 
-
|
 
-
|
 
-
|
 
-
|
 
-
|[[Paris/September 14| Sep. 14]]
 
-
|- style="background: #cccccc;"
 
-
| style="background: #ccffcc;" |D70
 
-
|pTet>>lox71-gfptripart FI
 
-
|(L34.1, L34.2) pTet>>lox71-gfptripart <bbpart>BBa_J61002</bbpart>
 
-
|EcoRI
 
-
|SpeI
 
-
|
 
-
|pTet>>lox71-gfptripart for use as a FI
 
-
|
 
-
|
 
-
|[[Paris/September 14| Sep. 14]]
 
-
|- style="background: #cccccc;"
 
-
| style="background: #ccffcc;" |D71
 
-
|araC/pBad>>lox71-gfptripart FI
 
-
|L35.1 araC/pBad>>lox71-gfptripart <bbpart>BBa_J61002</bbpart>
 
-
|EcoRI
 
-
|SpeI
 
-
|
 
-
|araC/pBad>>lox71-gfptripart for use as a FI
 
-
|Ø (24/09/07)
 
-
|
 
-
|[[Paris/September 14| Sep. 14]][[Paris/September 17| Sep. 17]]
 
-
|- style="background: #cccccc;"
 
-
| style="background: #FF99FF;" |D72
 
-
|<bbpart>pSB2K3</bbpart> BBvector
 
-
|MP16 <bbpart>pSB2K3</bbpart>::attP
 
-
|EcoRI
 
-
|PstI
 
-
|
 
-
|Low copy number plasmid, BB vector
 
-
|
 
-
|
 
-
|[[Paris/September 17| Sep. 17]]
 
-
|- style="background: #cccccc;"
 
-
| style="background: #FF99FF;" |D73
 
-
|<bbpart>pSB2K3</bbpart> ES open vector
 
-
|MP16 <bbpart>pSB2K3</bbpart>::attP
 
-
|EcoRI
 
-
|SpeI
 
-
|
 
-
|Comparison of digestion efficiency between Pst and Spe in NEB2
 
-
|
 
-
|
 
-
|[[Paris/September 17| Sep. 17]]
 
-
|- style="background: #cccccc;"
 
-
| style="background: #ccffcc;" |D76
 
-
|DGAT BB
 
-
|P14 DGAT
 
-
|EcoRI
 
-
|PstI
 
-
|
 
-
|DGAT
 
-
|Ø (24/09/07)
 
-
|
 
-
|[[Paris/September 17| Sep. 17]]
 
-
|- style="background: #cccccc;"
 
-
| style="background: #ccffcc;" |D77
 
-
|araC/pBad>>lox71-gfptripart BI
 
-
|D60 (.1 & .2) digestion product
 
-
|FspI
 
-
|AhdI
 
-
|
 
-
|in D60, the extracted fragment & the open plasmid have the same size, it is thus necessary to remove the plasmid by digestion
 
-
|
 
-
|
 
-
|[[Paris/September 17| Sep. 17]]
 
-
|- style="background: #cccccc;"
 
-
| style="background: #ccffcc;"  |D78
 
-
|araC/pBad>>lox71-gfptripart FI
 
-
|D71 (.1 & .2) digestion product
 
-
|FspI
 
-
|AhdI
 
-
|
 
-
|in D71, the extracted fragment & the open plasmid have the same size, it is thus necessary to remove the plasmid by digestion
 
-
|
 
-
|
 
-
|[[Paris/September 17| Sep. 17]]
 
-
|}
 
-
== Unexpected digestion results ==
+
<html>
-
{|
+
</div>
-
|- style="background: #ccccff;"
+
<div id="paris_content_boxtop">
-
! colspan="5" style="background: #ccccff;" | Unexpected results
+
</div>
-
|- style="background: #ccccff; text-align: center;"
+
<div id="paris_content">
-
|width=5%| Number
+
</html>
-
|width=20%| Matrix Name
+
-
|width=5%| Enzyme 1
+
-
|width=5%| Enzyme 2
+
-
|width=5%| Expected Size
+
-
|width=55%| Description
+
-
|width=5%| Date
+
-
|- style="background: #cccccc;"
+
-
| style="background: #ccffcc;" |D16
+
-
|<bbpart>pSB1AK3</bbpart>::<bbpart>B0015</bbpart> (MP3.1 & MP3.2)
+
-
|EcoR1
+
-
|Pst1
+
-
|~3000
+
-
|We have one band at ~2000. Furthermore, the strain which is suppose to be resistant to Kanamycin is not. We suspect that the part is in the pSB1A2 instead of the pSB1AK3
+
-
|[[Paris/July 21| July 21]]
+
-
|- style="background: #cccccc;"  
+
-
| style="background: #ccffcc;" |D17
+
-
|<bbpart>pSB1A2</bbpart>::<bbpart>E0422</bbpart> (MP6.1 & MP6.2)
+
-
|EcoRI
+
-
|PstI
+
-
|~2040 + ~920
+
-
|3 bands
+
-
|[[Paris/July 21| July 21]]
+
-
|- style="background: #cccccc;"
+
-
| style="background: #ccffcc;" |D25
+
-
|<bbpart>BBa_E0421</bbpart> (MP7.1 & MP7.2) PoPs to GFP converter
+
-
|XbaI
+
-
|PstI
+
-
|
+
-
|
+
-
|[[Paris/July 23| July 23]]
+
-
|- style="background: #cccccc;"  
+
-
| style="background: #FF99FF;" |D66
+
-
|(MP12.1, MP12.2) <bbpart>BBa_P1003</bbpart> in <bbpart>pSB1A2</bbpart>
+
-
|EcoR
+
-
|XbaI
+
-
|
+
-
|KanR <bbpart>BBa_J61025</bbpart> FV (for forward introduction of a casette for Wanner K insertion). The strange thing is that this digestion produces 2 visible products: ~1000 bp & ~2500 bp!!!
+
-
|[[Paris/September 17| Sep.17]], [[Paris/September 27| Sep.27]]
+
-
|}
+
-
== Strains (glycerol stock) ==
+
===Welcome===
-
{|
+
 
-
|- style="background: #ccccff;"
+
This is our wonderful Notebook, you will find here everything you need and don't need .
-
! colspan="5" style="background: #ccccff;" | Strains glycerol stock
+
 
-
|- style="background: #ccccff; text-align: center;"
+
 
-
|width=5%| Number
+
'''Hail to the Notebook !'''
-
|width=25%| Name
+
-
|width=55%| Description
+
-
|width=5%| Verified clone
+
-
|width=5%| Date
+
-
|- style="background: #cccccc;"
+
-
| style="background: #ccffcc;" |S1
+
-
|MG1655
+
-
|Standard E.Coli strain
+
-
|
+
-
|
+
-
|- style="background: #cccccc;"
+
-
| style="background: #ccffcc;" |S2
+
-
|Acinetobacter ADP1
+
-
|Strain used for TG coloration test with Red Nile
+
-
|
+
-
|
+
-
|- style="background: #cccccc;"
+
-
| style="background: #ccffcc;" |S3
+
-
|DH5alpha pKs::DGAT
+
-
|Enzyme catalysing the transormation of DAG into TG
+
-
|
+
-
|
+
-
|- style="background: #cccccc;"
+
-
| style="background: #ccffcc;" |S4
+
-
|<bbpart>BBa_pJ23100</bbpart>
+
-
|Strong constitutive promoter in J61002 (clones 1 + 2) <partinfo>pJ23100 SpecifiedComponents</partinfo>
+
-
|
+
-
|
+
-
|- style="background: #cccccc;"
+
-
| style="background: #ccffcc;" |S5
+
-
|<bbpart>BBa_B0015</bbpart>
+
-
|Double terminator (clones 1 + 2)    <partinfo>B0015 SpecifiedComponents</partinfo>
+
-
|
+
-
|
+
-
|- style="background: #cccccc;"
+
-
| style="background: #ccffcc;" |S6
+
-
|<bbpart>BBa_pJ23107</bbpart>
+
-
|Medium constitutive promoter in J61002 (clones 1 + 2) <partinfo>pJ23107 SpecifiedComponents</partinfo>
+
-
|
+
-
|[[Paris/July 19| July 19]]
+
-
|- style="background: #cccccc;"
+
-
| style="background: #ccffcc;" |S7
+
-
|<bbpart>BBa_B0030</bbpart>
+
-
|Strong RBS (clones 1 + 2) <partinfo>B0030 SpecifiedComponents</partinfo>
+
-
|
+
-
|[[Paris/July 19| July 19]]
+
-
|- style="background: #cccccc;"
+
-
| style="background: #ccffcc;" |S8
+
-
|<bbpart>BBa_E0422</bbpart>
+
-
|ECFP (RBS+LVA+Term) (clones 1 + 2) <partinfo>E0422 SpecifiedComponents</partinfo>
+
-
|
+
-
|[[Paris/July 19| July 19]]
+
-
|- style="background: #cccccc;"
+
-
| style="background: #ccffcc;" |S9
+
-
|<bbpart>BBa_E0241</bbpart>
+
-
|PoPs to GFP converter (clones 1 + 2) <partinfo>E0241 DeepComponents</partinfo>
+
-
|
+
-
|[[Paris/July 19| July 19]]
+
-
|- style="background: #cccccc;"
+
-
| style="background: #ccffcc;" |S10
+
-
|<bbpart>BBa_E0840</bbpart>
+
-
|gfp tri-part; strong rbs (clones 1 + 2) <partinfo>E0840 SpecifiedComponents</partinfo>
+
-
|
+
-
|[[Paris/July 19| July 19]]
+
-
|- style="background: #cccccc;"
+
-
| style="background: #ccffcc;" |S11
+
-
|<bbpart>BBa_J61047</bbpart>
+
-
|Cre ORF (clones 1 + 2) <partinfo>J61047 SpecifiedComponents</partinfo>
+
-
|
+
-
|[[Paris/July 19| July 19]]
+
-
|- style="background: #cccccc;"
+
-
| style="background: #ccffcc;" |S12
+
-
|W121
+
-
|
+
-
|
+
-
|05/07/07
+
-
|- style="background: #cccccc;"
+
-
| style="background: #ccffcc;" |S13
+
-
|<bbpart>BBa_I0500</bbpart>
+
-
|pBAD AraC (clone 1+2) <partinfo>I0500 SpecifiedComponents</partinfo>
+
-
|
+
-
|[[Paris/July 20| July 20]]
+
-
|- style="background: #cccccc;"
+
-
| style="background: #ccffcc;" |S14
+
-
|<bbpart>BBa_pJ23107</bbpart>
+
-
|Medium constitutive promoter in J61002 (clone 3+4) <partinfo>pJ23107 SpecifiedComponents</partinfo>
+
-
|
+
-
|[[Paris/July 20| July 20]]
+
-
|- style="background: #cccccc;"
+
-
| style="background: #ccffcc;" |S15
+
-
|<bbpart>BBa_P1003</bbpart>
+
-
|Kanamycin resistance cassette (clone 1+2) <partinfo>P1003 SpecifiedComponents</partinfo>
+
-
|
+
-
|[[Paris/July 20| July 20]]
+
-
|- style="background: #cccccc;"
+
-
| style="background: #ccffcc;" |S16
+
-
|<bbpart>J61002</bbpart>::<bbpart>I0500</bbpart>
+
-
|AraC pBad regulating mRFP expression in pJ61002 (L1) <partinfo>J61002 SpecifiedComponents</partinfo>
+
-
|L1.2, L1.3
+
-
|[[Paris/July 27| July 27]]
+
-
|- style="background: #cccccc;"
+
-
| style="background: #ccffcc;" |S17
+
-
|<bbpart>pSB1A2</bbpart>::Lox66-DapA Coli
+
-
|Lox66-DapA Coli cloned as a biobrick (L2)
+
-
|
+
-
|[[Paris/July 27| July 27]]
+
-
|- style="background: #cccccc;"
+
-
| style="background: #ccffcc;" |S18
+
-
|<bbpart>pSB1A2</bbpart>::B0030 + J61047
+
-
|RBS(B0030)-Cre ORF (L6) (clones 1+2)
+
-
|
+
-
|[[Paris/July 27| July 27]]
+
-
|- style="background: #cccccc;"
+
-
| style="background: #ccffcc;" |S19
+
-
|<bbpart>pSB1A2</bbpart>::<bbpart>BBa_J37026</bbpart>
+
-
|Lox66 (L9)(1 clone) <partinfo>J37026 SpecifiedComponents</partinfo>
+
-
|
+
-
|[[Paris/July 27| July 27]]
+
-
|- style="background: #cccccc;"
+
-
| style="background: #ccffcc;" |S20
+
-
|<bbpart>pSB1A2</bbpart>::<bbpart>BBa_J37028</bbpart>
+
-
|Lox71 (L10)(1 clone) <partinfo>J37028 SpecifiedComponents</partinfo>
+
-
|
+
-
|[[Paris/July 27| July 27]]
+
-
|- style="background: #cccccc;"
+
-
| style="background: #ccffcc;" |S21
+
-
|<bbpart>pSB1A2</bbpart>::<bbpart>BBa_J37028</bbpart>+<bbpart>BBa_E0840</bbpart>
+
-
|Lox71-gfp tri-part (L12)
+
-
|L12.2 ok
+
-
|?
+
-
|- style="background: #cccccc;"
+
-
| style="background: #ccffcc;" |S22
+
-
|<bbpart>pSB1A2</bbpart>::Lox71-kmR-lox66
+
-
|Lox71-kmR-lox66 (L25)
+
-
|sequence not good (thrown away)
+
-
|?
+
-
|- style="background: #cccccc;"
+
-
| style="background: #ccffcc;" |S23
+
-
|pTopo::P5
+
-
|Lox66-DapAColi
+
-
|
+
-
|?
+
-
|- style="background: #cccccc;"
+
-
| style="background: #ccffcc;" |S24
+
-
|pTopo::P6
+
-
|Lox66-DapASubtilis
+
-
|
+
-
|?
+
-
|- style="background: #cccccc;"
+
-
| style="background: #ccffcc;" |S25
+
-
|pTopo::P8
+
-
|RBS-DapAColi
+
-
|MPT4.3, MPT4.5
+
-
|?
+
-
|- style="background: #cccccc;"
+
-
| style="background: #ccffcc;" |S26
+
-
|pTopo::P9
+
-
|RBS-DapASubtilis
+
-
|
+
-
|?
+
-
|- style="background: #cccccc;"
+
-
| style="background: #ccffcc;" |S27
+
-
|<bbpart>J61002</bbpart>
+
-
|
+
-
|
+
-
|[[Paris/August 14| August 14]]
+
-
|- style="background: #cccccc;"
+
-
| style="background: #ccffcc;" |S28
+
-
|<bbpart>J61025</bbpart>
+
-
|FRT-Cm-FRT
+
-
|
+
-
|[[Paris/August 14| August 14]]
+
-
|- style="background: #cccccc;"
+
-
| style="background: #ccffcc;" |S29
+
-
|L26
+
-
|Insertion of RBS-Cre behind araC/pBad
+
-
|
+
-
|[[Paris/August 13| August 13]]
+
-
|- style="background: #cccccc;"
+
-
| style="background: #ccffcc;" |S30
+
-
|L27
+
-
|Insertion of ftsZ behind araC/pBad
+
-
|
+
-
|[[Paris/August 13| August 13]]
+
-
|- style="background: #cccccc;"
+
-
| style="background: #ccffcc;" |S31
+
-
|L28
+
-
|Insertion of ftsAZ behind araC/pBad
+
-
|
+
-
|[[Paris/August 13| August 13]]
+
-
|- style="background: #cccccc;"
+
-
| style="background: #ccffcc;" |S32
+
-
|L29
+
-
|Insertion of gfp-tripart in front of lox66
+
-
|
+
-
|[[Paris/August 13| August 13]]
+
-
|- style="background: #cccccc;"
+
-
| style="background: #ccffcc;" |S33A
+
-
|L30A
+
-
|cloning dapAp promotor in J61002
+
-
|
+
-
|[[Paris/August 13| August 13]]
+
-
|- style="background: #cccccc;"
+
-
| style="background: #ccffcc;" |S33B
+
-
|L30B
+
-
|cloning dapAp promotor in J61002
+
-
|
+
-
|[[Paris/August 13| August 13]]
+
-
|- style="background: #cccccc;"
+
-
| style="background: #ccffcc;" |S34
+
-
|L31
+
-
|Insertion of B0015 terminator behind lox71
+
-
|
+
-
|[[Paris/August 22| August 22]]
+
-
|- style="background: #cccccc;"
+
-
| style="background: #ccffcc;" |S35
+
-
|L32
+
-
|Insertion of B0015 terminator in front of lox66
+
-
|
+
-
|[[Paris/August 22| August 22]]
+
-
|- style="background: #cccccc;"
+
-
| style="background: #ccffcc;" |S36
+
-
|L34
+
-
|pTet>>lox71-gfptripart
+
-
|
+
-
|[[Paris/August 22| August 22]]
+
-
|- style="background: #cccccc;"
+
-
| style="background: #ccffcc;" |S37
+
-
|L35
+
-
|araC/pBad>>lox71-gfptripart
+
-
|
+
-
|[[Paris/August 22| August 22]]
+
-
|- style="background: #cccccc;"
+
-
| style="background: #ccffcc;" |S38
+
-
|L36
+
-
|lox66-mRFP
+
-
|
+
-
|[[Paris/August 22| August 22]]
+
-
|- style="background: #cccccc;"
+
-
| style="background: #ccffcc;" |S39
+
-
|L37
+
-
|lox66-RBS-dapAcoli BI
+
-
|
+
-
|[[Paris/August 22| August 22]]
+
-
|- style="background: #cccccc;"
+
-
| style="background: #ccffcc;" |S40
+
-
|L39
+
-
|RBS-dapAcoli cloned in pSB1A2
+
-
|
+
-
|[[Paris/August 22| August 22]]
+
-
|- style="background: #cccccc;"
+
-
| style="background: #ccffcc;" |S41
+
-
|L40
+
-
|RBS-dapAsubtilis cloned in pSB1A2
+
-
|
+
-
|[[Paris/August 22| August 22]]
+
-
|- style="background: #cccccc;"
+
-
| style="background: #ccffcc;" |S42
+
-
|L41
+
-
|lox71-ftsZ cloned in pSB1A2
+
-
|
+
-
|[[Paris/August 22| August 22]]
+
-
|- style="background: #cccccc;"
+
-
| style="background: #ccffcc;" |S43
+
-
|L42
+
-
|lox71-ftsA-Z cloned in pSB1A2
+
-
|
+
-
|[[Paris/August 22| August 22]]
+
-
|- style="background: #cccccc;"
+
-
| style="background: #ccffcc;" |S44
+
-
|L43
+
-
|RBS-dapAsubtilis cloned in pSB1A2
+
-
|
+
-
|[[Paris/August 22| August 22]]
+
-
|- style="background: #cccccc;"
+
-
| style="background: #ccffcc;" |S45A
+
-
|L30A
+
-
|DapAp>>mRFP within W121 strain
+
-
|
+
-
|[[Paris/August 25| August 25]]
+
-
|- style="background: #cccccc;"
+
-
| style="background: #ccffcc;" |S45B
+
-
|L30B
+
-
|DapAp>>mRFP within W121 strain
+
-
|
+
-
|[[Paris/August 25| August 25]]
+
-
|- style="background: #cccccc;"
+
-
| style="background: #ccffcc;" |S48
+
-
|pKD13
+
-
|FRT-KanR-FRT; Wanner plasmid
+
-
|
+
-
|[[Paris/September 18| Sep. 18]]
+
-
|- style="background: #cccccc;"
+
-
| style="background: #ccffcc;" |S49
+
-
|pKD46
+
-
|Red recombination system; Wanner plasmid
+
-
|
+
-
|[[Paris/September_13| Sep. 13]]
+
-
|- style="background: #cccccc;"
+
-
| style="background: #ccffcc;" |S50
+
-
|L30 plasmid in FR781
+
-
|dapAp>>mRFP plasmid
+
-
|
+
-
|[[Paris/September_28| Sep. 28]]
+
-
|}
+

Latest revision as of 13:39, 28 August 2009

iGEM > Paris > NoteBook

NoteBook

June
MTWTFSS
[http://2009.igem.org/Team:Paris/1_June_2009 1] [http://2009.igem.org/wiki/index.php?title=Team:Paris/2_June_2009&action=edit 2] [http://2009.igem.org/wiki/index.php?title=Team:Paris/3_June_2009&action=edit 3] [http://2009.igem.org/wiki/index.php?title=Team:Paris/4_June_2009&action=edit 4] [http://2009.igem.org/wiki/index.php?title=Team:Paris/5_June_2009&action=edit 5] [http://2009.igem.org/wiki/index.php?title=Team:Paris/6_June_2009&action=edit 6] [http://2009.igem.org/wiki/index.php?title=Team:Paris/7_June_2009&action=edit 7]
[http://2009.igem.org/wiki/index.php?title=Team:Paris/8_June_2009&action=edit 8] [http://2009.igem.org/wiki/index.php?title=Team:Paris/9_June_2009&action=edit 9] [http://2009.igem.org/wiki/index.php?title=Team:Paris/10_June_2009&action=edit 10] [http://2009.igem.org/wiki/index.php?title=Team:Paris/11_June_2009&action=edit 11] [http://2009.igem.org/wiki/index.php?title=Team:Paris/12_June_2009&action=edit 12] [http://2009.igem.org/Team:Paris/13_June_2009 13] [http://2009.igem.org/wiki/index.php?title=Team:Paris/14_June_2009&action=edit 14]
[http://2009.igem.org/wiki/index.php?title=Team:Paris/15_June_2009&action=edit 15] [http://2009.igem.org/wiki/index.php?title=Team:Paris/16_June_2009&action=edit 16] [http://2009.igem.org/wiki/index.php?title=Team:Paris/17_June_2009&action=edit 17] [http://2009.igem.org/wiki/index.php?title=Team:Paris/18_June_2009&action=edit 18] [http://2009.igem.org/wiki/index.php?title=Team:Paris/19_June_2009&action=edit 19] [http://2009.igem.org/wiki/index.php?title=Team:Paris/20_June_2009&action=edit 20] [http://2009.igem.org/wiki/index.php?title=Team:Paris/21_June_2009&action=edit 21]
[http://2009.igem.org/wiki/index.php?title=Team:Paris/22_June_2009&action=edit 22] [http://2009.igem.org/Team:Paris/23_June_2009 23] [http://2009.igem.org/wiki/index.php?title=Team:Paris/24_June_2009&action=edit 24] [http://2009.igem.org/wiki/index.php?title=Team:Paris/25_June_2009&action=edit 25] [http://2009.igem.org/wiki/index.php?title=Team:Paris/26_June_2009&action=edit 26] [http://2009.igem.org/wiki/index.php?title=Team:Paris/27_June_2009&action=edit 27] [http://2009.igem.org/wiki/index.php?title=Team:Paris/28_June_2009&action=edit 28]
[http://2009.igem.org/wiki/index.php?title=Team:Paris/29_June_2009&action=edit 29] [http://2009.igem.org/Team:Paris/30_June_2009 30]
July
MTWTFSS
    [http://2009.igem.org/wiki/index.php?title=Team:Paris/1_July_2009&action=edit 1] [http://2009.igem.org/wiki/index.php?title=Team:Paris/2_July_2009&action=edit 2] [http://2009.igem.org/Team:Paris/3_July_2009 3] [http://2009.igem.org/wiki/index.php?title=Team:Paris/4_July_2009&action=edit 4] [http://2009.igem.org/wiki/index.php?title=Team:Paris/5_July_2009&action=edit 5]
[http://2009.igem.org/wiki/index.php?title=Team:Paris/6_July_2009&action=edit 6] [http://2009.igem.org/wiki/index.php?title=Team:Paris/7_July_2009&action=edit 7] [http://2009.igem.org/wiki/index.php?title=Team:Paris/8_July_2009&action=edit 8] [http://2009.igem.org/wiki/index.php?title=Team:Paris/9_July_2009&action=edit 9] [http://2009.igem.org/wiki/index.php?title=Team:Paris/10_July_2009&action=edit 10] [http://2009.igem.org/wiki/index.php?title=Team:Paris/11_July_2009&action=edit 11] [http://2009.igem.org/wiki/index.php?title=Team:Paris/12_July_2009&action=edit 12]
[http://2009.igem.org/Team:Paris/13_July_2009 13] [http://2009.igem.org/Team:Paris/14_July_2009 14] [http://2009.igem.org/Team:Paris/15_July_2009 15] [http://2009.igem.org/Team:Paris/16_July_2009 16] [http://2009.igem.org/Team:Paris/17_July_2009 17] [http://2009.igem.org/Team:Paris/18_July_2009 18] [http://2009.igem.org/Team:Paris/19_July_2009 19]
[http://2009.igem.org/Team:Paris/20_July_2009 20] [http://2009.igem.org/Team:Paris/21_July_2009 21] [http://2009.igem.org/Team:Paris/22_July_2009 22] [http://2009.igem.org/Team:Paris/23_July_2009 23] [http://2009.igem.org/Team:Paris/24_July_2009 24] [http://2009.igem.org/Team:Paris/25_July_2009 25] [http://2009.igem.org/Team:Paris/26_July_2009 26]
[http://2009.igem.org/Team:Paris/27_July_2009 27] [http://2009.igem.org/Team:Paris/28_July_2009 28] [http://2009.igem.org/Team:Paris/29_July_2009 29] [http://2009.igem.org/Team:Paris/30_July_2009 30] [http://2009.igem.org/Team:Paris/31_July_2009 31]
August
MTWTFSS
          [http://2009.igem.org/Team:Paris/1_August_2009 1] [http://2009.igem.org/Team:Paris/2_August_2009 2]
[http://2009.igem.org/Team:Paris/3_August_2009 3] [http://2009.igem.org/Team:Paris/4_August_2009 4] [http://2009.igem.org/Team:Paris/5_August_2009 5] [http://2009.igem.org/Team:Paris/6_August_2009 6] [http://2009.igem.org/Team:Paris/7_August_2009 7] [http://2009.igem.org/Team:Paris/8_August_2009 8] [http://2009.igem.org/Team:Paris/9_August_2009 9]
[http://2009.igem.org/Team:Paris/10_August_2009 10] [http://2009.igem.org/Team:Paris/11_August_2009 11] [http://2009.igem.org/Team:Paris/12_August_2009 12] [http://2009.igem.org/Team:Paris/13_August_2009 13] [http://2009.igem.org/Team:Paris/14_August_2009 14] [http://2009.igem.org/Team:Paris/15_August_2009 15] [http://2009.igem.org/Team:Paris/16_August_2009 16]
[http://2009.igem.org/Team:Paris/17_August_2009 17] [http://2009.igem.org/Team:Paris/18_August_2009 18] [http://2009.igem.org/Team:Paris/19_August_2009 19] [http://2009.igem.org/Team:Paris/20_August_2009 20] [http://2009.igem.org/Team:Paris/21_August_2009 21] [http://2009.igem.org/Team:Paris/22_August_2009 22] [http://2009.igem.org/Team:Paris/23_August_2009 23]
[http://2009.igem.org/Team:Paris/24_August_2009 24] [http://2009.igem.org/Team:Paris/25_August_2009 25] [http://2009.igem.org/Team:Paris/26_August_2009 26] [http://2009.igem.org/Team:Paris/27_August_2009 27] [http://2009.igem.org/Team:Paris/28_August_2009 28] [http://2009.igem.org/Team:Paris/29_August_2009 29] [http://2009.igem.org/Team:Paris/30_August_2009 30]
[http://2009.igem.org/Team:Paris/31_August_2009 31]
September
MTWTFSS
  [http://2009.igem.org/Team:Paris/1_September_2009 1] [http://2009.igem.org/Team:Paris/2_September_2009 2] [http://2009.igem.org/Team:Paris/3_September_2009 3] [http://2009.igem.org/Team:Paris/4_September_2009 4] [http://2009.igem.org/Team:Paris/5_September_2009 5] [http://2009.igem.org/Team:Paris/6_September_2009 6]
[http://2009.igem.org/Team:Paris/7_September_2009 7] [http://2009.igem.org/Team:Paris/8_September_2009 8] [http://2009.igem.org/Team:Paris/9_September_2009 9] [http://2009.igem.org/Team:Paris/10_September_2009 10] [http://2009.igem.org/Team:Paris/11_September_2009 11] [http://2009.igem.org/Team:Paris/12_September_2009 12] [http://2009.igem.org/Team:Paris/13_September_2009 13]
[http://2009.igem.org/Team:Paris/14_September_2009 14] [http://2009.igem.org/Team:Paris/15_September_2009 15] [http://2009.igem.org/Team:Paris/16_September_2009 16] [http://2009.igem.org/Team:Paris/17_September_2009 17] [http://2009.igem.org/Team:Paris/18_September_2009 18] [http://2009.igem.org/Team:Paris/19_September_2009 19] [http://2009.igem.org/Team:Paris/20_September_2009 20]
[http://2009.igem.org/Team:Paris/21_September_2009 21] [http://2009.igem.org/Team:Paris/22_September_2009 22] [http://2009.igem.org/Team:Paris/23_September_2009 23] [http://2009.igem.org/Team:Paris/24_September_2009 24] [http://2009.igem.org/Team:Paris/25_September_2009 25] [http://2009.igem.org/Team:Paris/26_September_2009 26] [http://2009.igem.org/Team:Paris/27_September_2009 27]
[http://2009.igem.org/Team:Paris/28_September_2009 28] [http://2009.igem.org/Team:Paris/29_September_2009 29] [http://2009.igem.org/Team:Paris/30_September_2009 30]
October
MTWTFSS
      [http://2009.igem.org/Team:Paris/1_October_2009 1] [http://2009.igem.org/Team:Paris/2_October_2009 2] [http://2009.igem.org/Team:Paris/3_October_2009 3] [http://2009.igem.org/Team:Paris/4_October_2009 4]
[http://2009.igem.org/Team:Paris/5_October_2009 5] [http://2009.igem.org/Team:Paris/6_October_2009 6] [http://2009.igem.org/Team:Paris/7_October_2009 7] [http://2009.igem.org/Team:Paris/8_October_2009 8] [http://2009.igem.org/Team:Paris/9_October_2009 9] [http://2009.igem.org/Team:Paris/10_October_2009 10] [http://2009.igem.org/Team:Paris/11_October_2009 11]
[http://2009.igem.org/Team:Paris/12_October_2009 12] [http://2009.igem.org/Team:Paris/13_October_2009 13] [http://2009.igem.org/Team:Paris/14_October_2009 14] [http://2009.igem.org/Team:Paris/15_October_2009 15] [http://2009.igem.org/Team:Paris/16_October_2009 16] [http://2009.igem.org/Team:Paris/17_October_2009 17] [http://2009.igem.org/Team:Paris/18_October_2009 18]
[http://2009.igem.org/Team:Paris/19_October_2009 19] [http://2009.igem.org/Team:Paris/20_October_2009 20] [http://2009.igem.org/Team:Paris/21_October_2009 21] [http://2009.igem.org/Team:Paris/22_October_2009 22] [http://2009.igem.org/Team:Paris/23_October_2009 23] [http://2009.igem.org/Team:Paris/24_October_2009 24] [http://2009.igem.org/Team:Paris/25_October_2009 25]
[http://2009.igem.org/Team:Paris/26_October_2009 26] [http://2009.igem.org/Team:Paris/27_October_2009 27] [http://2009.igem.org/Team:Paris/28_October_2009 28] [http://2009.igem.org/Team:Paris/29_October_2009 29] [http://2009.igem.org/Team:Paris/30_October_2009 30] [http://2009.igem.org/Team:Paris/31_October_2009 31]


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