Team:Paris/24 August 2009
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==NoteBook== | ==NoteBook== | ||
- | { | + | {{Paris2009_Calendar}} |
- | + | {{Paris2009_Calendar_Link|23_August_2009|25_August_2009}} | |
- | + | <center> '''August 24th''' </center> | |
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<html> | <html> | ||
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</html> | </html> | ||
+ | ==Lab work== | ||
- | == | + | <div class="caroline"> |
+ | Ligation Tranfo | ||
+ | </div> | ||
+ | <div class="experience"> | ||
+ | OmpAsignal A28 : pSB1A3 | ||
+ | 15min 20° then transfo // O/N 16° | ||
+ | </div> | ||
+ | <div class="caroline"> | ||
+ | gel purif | ||
+ | </div> | ||
+ | <div class="experience"> | ||
+ | A40 TolRII | ||
+ | </div> | ||
- | + | <div class="vicard"> | |
+ | Transformation check | ||
+ | </div> | ||
+ | <div class="experience"> | ||
+ | D40+PSB1A3->Nter RFP-->worked | ||
- | + | D41+PSB1A3->RFP-->worked | |
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
+ | D42+PSB1A3->Cter RFP-->worked | ||
- | + | negativ control OK | |
- | + | </div> | |
- | < | + | <div class="vicard"> |
+ | PCR on colonies | ||
</div> | </div> | ||
- | <div | + | <div class="experience"> |
- | + | ||
- | + | ||
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+ | D40+PSB1A3->Nter RFP-->ok | ||
- | + | D41+PSB1A3->RFP-->not ok but don't need | |
- | + | D42+PSB1A3->Cter RFP-->ok | |
+ | |||
+ | negativ control OK | ||
+ | <center>[[Image:RFP_22aout.JPG]]</center> | ||
+ | |||
+ | |||
+ | </div> |
Latest revision as of 22:59, 16 October 2009
NoteBook
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Lab work
Ligation Tranfo
OmpAsignal A28 : pSB1A3
15min 20° then transfo // O/N 16°
gel purif
A40 TolRII
Transformation check
D40+PSB1A3->Nter RFP-->worked
D41+PSB1A3->RFP-->worked
D42+PSB1A3->Cter RFP-->worked
negativ control OK
PCR on colonies