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EPF-Lausanne/12 August 2009
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==Wet Lab== | ==Wet Lab== | ||
| + | The plates from yesterday's transformation had A LOT of clones so we did a colony PCR of about 15 clones per plate to check the efficiency of the ligation. | ||
| + | Redid a normal PCR on the 1.5-step PCR products since concentrations weren't high enough. | ||
| + | |||
| + | Prepared LB-Agar plates with ampicillin and kanamycin antibiotics. | ||
| + | |||
| + | Prepared a tetracycline stock (to use as an inducer). Cells were left to grow in LB medium with different concentrations of this stock at 37°C as a test for their growth speed: OD checked every hour. | ||
| + | |||
| + | We did a ligation of our read out system 2 during 1h and we did a transformation with the obtained products. Plates were left overnight at 37°C. | ||
==People in the lab== | ==People in the lab== | ||
| - | Nath, Basile, Gab, Christian | + | Nath, Basile, Gab, Christian, Nicolas |
Latest revision as of 08:31, 18 August 2009
Contents |
Wet Lab
The plates from yesterday's transformation had A LOT of clones so we did a colony PCR of about 15 clones per plate to check the efficiency of the ligation.
Redid a normal PCR on the 1.5-step PCR products since concentrations weren't high enough.
Prepared LB-Agar plates with ampicillin and kanamycin antibiotics.
Prepared a tetracycline stock (to use as an inducer). Cells were left to grow in LB medium with different concentrations of this stock at 37°C as a test for their growth speed: OD checked every hour.
We did a ligation of our read out system 2 during 1h and we did a transformation with the obtained products. Plates were left overnight at 37°C.
People in the lab
Nath, Basile, Gab, Christian, Nicolas
