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| + | <span/ id="bottom">[https://2009.igem.org/ iGEM ] > [[Team:Paris#top | Paris]] > [[Team:Paris/Freezer#top | Freezer]] > [[Team:Paris/Freezer#bottom | Main]] |
| {{Template:Paris2009}} | | {{Template:Paris2009}} |
| {{Template:Paris2009_menu}} | | {{Template:Paris2009_menu}} |
| + | |
| | | |
| ==Freezer== | | ==Freezer== |
- | *[[Team:Paris/Freezer#Primers | Primers]]
| |
- | *[[Team:Paris/Freezer#PCR_products | PCR products]]
| |
- | *[[Team:Paris/Freezer#Plasmids:_MiniPrep_Products | Plasmids: MiniPrep Products]]
| |
- | *[[Team:Paris/Freezer#Digestion_Products | Digestion Products]]
| |
- | *[[Team:Paris/Freezer#Unexpected_digestion_results | Unexpected digestion results]]
| |
- | *[[Team:Paris/Freezer#Strains_(Glycerol stock) | Strains (Glycerol stock)]]
| |
- |
| |
- |
| |
- | *Color code for sticker
| |
- | **<span style="background-color:#FF0000">Vector</span>
| |
- | **<span style="background-color:#FFFF00">Insert</span>
| |
- | **<span style="background-color:#660099">PCR product</span>
| |
- | **<span style="background-color:#FF6600">Plasmid</span>
| |
- | **<span style="background-color:#006400">Kanamycin</span>
| |
- | **<span style="background-color:#3333FF">Ampicilin</span>
| |
- | **<span style="background-color:#FF0066">Chloranphenicol</span>
| |
| <html> | | <html> |
- | </div> | + | <style type="text/css"> |
- | <div id="paris_content_boxtop">
| + | #left-side { |
- | </div>
| + | position: absolute; |
- | <div id="paris_content">
| + | height: 23px; |
- | </html>
| + | width: 30px; |
| + | top: 0px; |
| + | left: 10px; |
| + | margin-top:10px; |
| + | padding-top: 7px; |
| + | background: url(https://static.igem.org/mediawiki/2009/1/1b/Left_menu_pari.png); |
| + | z-index:4; |
| + | } |
| | | |
- | == Primers ==
| + | #middle-side { |
- | {| | + | height: 25px; |
- | ! colspan="6" style="background: #c5c5c5;" | [O1] Rev P1
| + | width: 650px; |
- | |- style="background: #cbcee0;"
| + | position: absolute; |
- | |style="background:#0d3e99; color:white; text-align: right;" width=120px|Description
| + | top: 0px; |
- | |width=700px| tolR
| + | left: 30px; |
- | |- style="background: #cbcee0;"
| + | margin-top:10px; |
- | |style="background:#0d3e99; color:white; text-align: right;"|Sequence (5' > 3')
| + | padding-top: 5px; |
- | |TCTGCTGCAGCGGCCGCTACTAGTATTAGTAAGGCACATCTTTTGCGCCACCG
| + | background: #dadada; |
- | |- style="background: #cbcee0;"
| + | z-index:5; |
- | |style="background:#0d3e99; color:white; text-align: right;"|Designer
| + | } |
- | |D. Bikard
| + | |
- | |- style="background: #cbcee0;"
| + | |
- | !colspan="6" style="background:#c5c5c5; text-align: right;" | Worked
| + | |
- | |}
| + | |
| | | |
| + | #right-side { |
| + | position: absolute; |
| + | height: 23px; |
| + | width: 30px; |
| + | margin-top:10px; |
| + | padding-top: 7px; |
| + | top: 0px; |
| + | left: 670px; |
| + | background: url(https://static.igem.org/mediawiki/2009/4/40/Right_menu_paris.png); |
| + | z-index:4; |
| + | } |
| | | |
- | {| | + | a.menu_sub { |
- | ! colspan="6" style="background: #c5c5c5;" | [O2] Fwd P2
| + | padding-left: 7px; |
- | |- style="background: #cbcee0;"
| + | padding-right: 7px; |
- | |style="background:#0d3e99; color:white; text-align: right;" width=120px|Description
| + | } |
- | |width=700px| tolR (DO NOT USE ERROR)
| + | |
- | |- style="background: #cbcee0;"
| + | |
- | |style="background:#0d3e99; color:white; text-align: right;"|Sequence (5' > 3')
| + | |
- | |TCTGGAATTCGCGGCCGCTTCTAGATGAAATCAACATTGTACCGTTGCTGGACGTACTG
| + | |
- | |- style="background: #cbcee0;"
| + | |
- | |style="background:#0d3e99; color:white; text-align: right;"|Designer
| + | |
- | |D. Bikard
| + | |
- | |- style="background: #cbcee0;"
| + | |
- | !colspan="6" style="background:#c5c5c5; text-align: right;" | Worked
| + | |
- | |}
| + | |
| | | |
| + | a.menu_sub_active { |
| + | padding-left: 7px; |
| + | padding-right: 7px; |
| + | color:#b0310e; |
| + | font-weight:bold; |
| + | } |
| + | </style> |
| + | <div id="left-side"></div> |
| + | <div id="middle-side"><center> |
| + | <a class="menu_sub_active"href="https://2009.igem.org/Team:Paris/Freezer#bottom"> Main </a>| |
| + | <a class="menu_sub" href="https://2009.igem.org/Team:Paris/Freezer_PCR_products#bottom"> PCR Products</a>| |
| + | <a class="menu_sub"href="https://2009.igem.org/Team:Paris/Freezer_Plasmids#bottom"> Plasmids</a>| |
| + | <a class="menu_sub"href="https://2009.igem.org/Team:Paris/Freezer_Digestion_Products#bottom"> Digestion Products</a>| |
| + | <a class="menu_sub"href="https://2009.igem.org/Team:Paris/Freezer_Strains_(Glycerol_stock)#bottom"> Strains (Glycerol stock)</a>| |
| + | <a class="menu_sub"href="https://2009.igem.org/Team:Paris/constructions#bottom"> Constructions in progress</a> |
| + | </center> |
| + | </div> |
| | | |
- | {|
| + | <div id="right-side"></div> |
- | ! colspan="6" style="background: #c5c5c5;" | [O3] Rev P3
| + | |
- | |- style="background: #cbcee0;"
| + | |
- | |style="background:#0d3e99; color:white; text-align: right;" width=120px|Description
| + | |
- | |width=700px| tolA
| + | |
- | |- style="background: #cbcee0;"
| + | |
- | |style="background:#0d3e99; color:white; text-align: right;"|Sequence (5' > 3')
| + | |
- | |TCTGCTGCAGCGGCCGCTACTAGTATTATTACGGTTTGAAGTCCAATGGCG
| + | |
- | |- style="background: #cbcee0;"
| + | |
- | |style="background:#0d3e99; color:white; text-align: right;"|Designer
| + | |
- | |D. Bikard
| + | |
- | |- style="background: #cbcee0;"
| + | |
- | !colspan="6" style="background:#c5c5c5; text-align: right;" | Worked
| + | |
- | |}
| + | |
| | | |
- |
| |
- | {|
| |
- | ! colspan="6" style="background: #c5c5c5;" | [O4] Rev P4
| |
- | |- style="background: #cbcee0;"
| |
- | |style="background:#0d3e99; color:white; text-align: right;" width=120px|Description
| |
- | |width=700px| tolA (ERROR: Not designed for N-term fusion)
| |
- | |- style="background: #cbcee0;"
| |
- | |style="background:#0d3e99; color:white; text-align: right;"|Sequence (5' > 3')
| |
- | |TCTGGAATTCGCGGCCGCTTCTAGAATGGGTAATACTAAAAACAATGGCGC
| |
- | |- style="background: #cbcee0;"
| |
- | |style="background:#0d3e99; color:white; text-align: right;"|Designer
| |
- | |D. Bikard
| |
- | |- style="background: #cbcee0;"
| |
- | !colspan="6" style="background:#c5c5c5; text-align: right;" | Worked
| |
- | |}
| |
- |
| |
- |
| |
- | {|
| |
- | ! colspan="6" style="background: #c5c5c5;" | [O5] Rev P5
| |
- | |- style="background: #cbcee0;"
| |
- | |style="background:#0d3e99; color:white; text-align: right;" width=120px|Description
| |
- | |width=700px| tatABC
| |
- | |- style="background: #cbcee0;"
| |
- | |style="background:#0d3e99; color:white; text-align: right;"|Sequence (5' > 3')
| |
- | |TCTGCTGCAGCGGCCGCTACTAGTATTATTATTCTTCAGTTTTTTCGCTTTCTGC
| |
- | |- style="background: #cbcee0;"
| |
- | |style="background:#0d3e99; color:white; text-align: right;"|Designer
| |
- | |D. Bikard
| |
- | |- style="background: #cbcee0;"
| |
- | !colspan="6" style="background:#c5c5c5; text-align: right;" | Worked
| |
- | |}
| |
- |
| |
- |
| |
- | {|
| |
- | ! colspan="6" style="background: #c5c5c5;" | [06] Fwd P6
| |
- | |- style="background: #cbcee0;"
| |
- | |style="background:#0d3e99; color:white; text-align: right;" width=120px|Description
| |
- | |width=700px| tatABC (without RBS)
| |
- | |- style="background: #cbcee0;"
| |
- | |style="background:#0d3e99; color:white; text-align: right;"|Sequence (5' > 3')
| |
- | |TCTGGAATTCGCGGCCGCTTCTAGAATGGGTGGTATCAGTATTTGG
| |
- | |- style="background: #cbcee0;"
| |
- | |style="background:#0d3e99; color:white; text-align: right;"|Designer
| |
- | |D. Bikard
| |
- | |- style="background: #cbcee0;"
| |
- | !colspan="6" style="background:#c5c5c5; text-align: right;" | Worked
| |
- | |}
| |
- |
| |
- |
| |
- |
| |
- | {|
| |
- | ! colspan="6" style="background: #c5c5c5;" | [O7] Rev P7
| |
- | |- style="background: #cbcee0;"
| |
- | |style="background:#0d3e99; color:white; text-align: right;" width=120px|Description
| |
- | |width=700px| clyA fusion
| |
- | |- style="background: #cbcee0;"
| |
- | |style="background:#0d3e99; color:white; text-align: right;"|Sequence (5' > 3')
| |
- | |TTTCTGCAGCGGCCGCTACTAGTGACTTCAGGTACCTCAAAGAGTGTC
| |
- | |- style="background: #cbcee0;"
| |
- | |style="background:#0d3e99; color:white; text-align: right;"|Designer
| |
- | |D. Bikard
| |
- | |- style="background: #cbcee0;"
| |
- | !colspan="6" style="background:#c5c5c5; text-align: right;" | Worked
| |
- | |}
| |
- |
| |
- |
| |
- |
| |
- |
| |
- | {|
| |
- | ! colspan="6" style="background: #c5c5c5;" | [O8] Fwd P8
| |
- | |- style="background: #cbcee0;"
| |
- | |style="background:#0d3e99; color:white; text-align: right;" width=120px|Description
| |
- | |width=700px| clyA fusion (ERROR: No poly-Gly linker)
| |
- | |- style="background: #cbcee0;"
| |
- | |style="background:#0d3e99; color:white; text-align: right;"|Sequence (5' > 3')
| |
- | |TTTGAATTCGCGGCCGCTTCTAGAATGACTGAAATCGTTGCAGATAAAA
| |
- | |- style="background: #cbcee0;"
| |
- | |style="background:#0d3e99; color:white; text-align: right;"|Designer
| |
- | |D. Bikard
| |
- | |- style="background: #cbcee0;"
| |
- | !colspan="6" style="background:#c5c5c5; text-align: right;" | Worked
| |
- | |}
| |
- |
| |
- |
| |
- | {|
| |
- | ! colspan="6" style="background: #c5c5c5;" | [O9] clyA_Rw
| |
- | |- style="background: #cbcee0;"
| |
- | |style="background:#0d3e99; color:white; text-align: right;" width=120px|Description
| |
- | |width=700px| clyA
| |
- | |- style="background: #cbcee0;"
| |
- | |style="background:#0d3e99; color:white; text-align: right;"|Sequence (5' > 3')
| |
- | |GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTAGACTTCAGGTACCTCAAAGAG
| |
- | |- style="background: #cbcee0;"
| |
- | |style="background:#0d3e99; color:white; text-align: right;"|Designer
| |
- | |G. Cambray
| |
- | |- style="background: #cbcee0;"
| |
- | !colspan="6" style="background:#c5c5c5; text-align: right;" | Not Tested
| |
- | |}
| |
- |
| |
- |
| |
- |
| |
- | {|
| |
- | ! colspan="6" style="background: #c5c5c5;" | [O10] clyA_fusion_polyG_Rw
| |
- | |- style="background: #cbcee0;"
| |
- | |style="background:#0d3e99; color:white; text-align: right;" width=120px|Description
| |
- | |width=700px| clyA fusion (with 5-Gly linker)
| |
- | |- style="background: #cbcee0;"
| |
- | |style="background:#0d3e99; color:white; text-align: right;"|Sequence (5' > 3')
| |
- | |GTTTCTTCCTGCAGCGGCCGCTACTAGTGCCGCCGCCGCCGCCGACTTCAGGTACCTCAAAGAG
| |
- | |- style="background: #cbcee0;"
| |
- | |style="background:#0d3e99; color:white; text-align: right;"|Designer
| |
- | |G. Cambray
| |
- | |- style="background: #cbcee0;"
| |
- | !colspan="6" style="background:#c5c5c5; text-align: right;" | Not Tested
| |
- | |}
| |
- |
| |
- |
| |
- |
| |
- | {|
| |
- | ! colspan="6" style="background: #c5c5c5;" | [O11] ompA_signal_Fw
| |
- | |- style="background: #cbcee0;"
| |
- | |style="background:#0d3e99; color:white; text-align: right;" width=120px|Description
| |
- | |width=700px| ompA signal for export to periplasm - from expression vector RBS as in pIN-III-ompA-2 vector - keeps vector original RBS
| |
- | |- style="background: #cbcee0;"
| |
- | |style="background:#0d3e99; color:white; text-align: right;"|Sequence (5' > 3')
| |
- | |GTTTCTTCGAATTCGCGGCCGCTTCTAGAGAACGAGGGCAAAAAATGAAAAAG
| |
- | |- style="background: #cbcee0;"
| |
- | |style="background:#0d3e99; color:white; text-align: right;"|Designer
| |
- | |G. Cambray
| |
- | |- style="background: #cbcee0;"
| |
- | !colspan="6" style="background:#c5c5c5; text-align: right;" | Not Tested
| |
- | |}
| |
- |
| |
- |
| |
- |
| |
- | {|
| |
- | ! colspan="6" style="background: #c5c5c5;" | [O12] ompA_signal_fusion_Rw
| |
- | |- style="background: #cbcee0;"
| |
- | |style="background:#0d3e99; color:white; text-align: right;" width=120px|Description
| |
- | |width=700px| ompA signal for export to periplasm - from expression vector pIN-III-ompA-2 - original RBS from the vector
| |
- | |- style="background: #cbcee0;"
| |
- | |style="background:#0d3e99; color:white; text-align: right;"|Sequence (5' > 3')
| |
- | |GTTTCTTCCTGCAGCGGCCGCTACTAGTGGCCTGCGCTACGGTAGCGAAAC
| |
- | |- style="background: #cbcee0;"
| |
- | |style="background:#0d3e99; color:white; text-align: right;"|Designer
| |
- | |G. Cambray
| |
- | |- style="background: #cbcee0;"
| |
- | !colspan="6" style="background:#c5c5c5; text-align: right;" | Not Tested
| |
- | |}
| |
- |
| |
- |
| |
- | {|
| |
- | ! colspan="6" style="background: #c5c5c5;" | [O13] tolR_II_Fusion_Fw
| |
- | |- style="background: #cbcee0;"
| |
- | |style="background:#0d3e99; color:white; text-align: right;" width=120px|Description
| |
- | |width=700px| For fusion of tolR domain II (standard 23)
| |
- | |- style="background: #cbcee0;"
| |
- | |style="background:#0d3e99; color:white; text-align: right;"|Sequence (5' > 3')
| |
- | |GTTTCTTCGAATTCGCGGCCGCTTCTAGAGAGGTCGATCTGCCAGACGCTAC
| |
- | |- style="background: #cbcee0;"
| |
- | |style="background:#0d3e99; color:white; text-align: right;"|Designer
| |
- | |G. Cambray
| |
- | |- style="background: #cbcee0;"
| |
- | !colspan="6" style="background:#c5c5c5; text-align: right;" | Not Tested
| |
- | |}
| |
- |
| |
- |
| |
- | {|
| |
- | ! colspan="6" style="background: #c5c5c5;" | [O14] tatABC_RBS_Fw
| |
- | |- style="background: #cbcee0;"
| |
- | |style="background:#0d3e99; color:white; text-align: right;" width=120px|Description
| |
- | |width=700px| Amplification from tatA - contains RBS
| |
- | |- style="background: #cbcee0;"
| |
- | |style="background:#0d3e99; color:white; text-align: right;"|Sequence (5' > 3')
| |
- | |TTGAATTCGCGGCCGCTTCTAGAAGGAGGTATATAATGATGGGTGGTATCAGTATTTGG
| |
- | |- style="background: #cbcee0;"
| |
- | |style="background:#0d3e99; color:white; text-align: right;"|Designer
| |
- | |G. Cambray
| |
- | |- style="background: #cbcee0;"
| |
- | !colspan="6" style="background:#c5c5c5; text-align: right;" | Not Tested
| |
- | |}
| |
- |
| |
- |
| |
- | {|
| |
- | ! colspan="6" style="background: #c5c5c5;" | [O15] TE3_fusion_Fw
| |
- | |- style="background: #cbcee0;"
| |
- | |style="background:#0d3e99; color:white; text-align: right;" width=120px|Description
| |
- | |width=700px| Fusion of N-term domain of colicin E3 (standard 23)
| |
- | |- style="background: #cbcee0;"
| |
- | |style="background:#0d3e99; color:white; text-align: right;"|Sequence (5' > 3')
| |
- | |GTTTCTTCGAATTCGCGGCCGCTTCTAGAAGCGGTGGCGATGGACGCGGCCATAAC
| |
- | |- style="background: #cbcee0;"
| |
- | |style="background:#0d3e99; color:white; text-align: right;"|Designer
| |
- | |G. Cambray
| |
- | |- style="background: #cbcee0;"
| |
- | !colspan="6" style="background:#c5c5c5; text-align: right;" | Not Tested
| |
- | |}
| |
- |
| |
- |
| |
- | {|
| |
- | ! colspan="6" style="background: #c5c5c5;" | [O16] TE3_Rw
| |
- | |- style="background: #cbcee0;"
| |
- | |style="background:#0d3e99; color:white; text-align: right;" width=120px|Description
| |
- | |width=700px| Amplification of N-term domain of colicin E3 (standard 23)
| |
- | |- style="background: #cbcee0;"
| |
- | |style="background:#0d3e99; color:white; text-align: right;"|Sequence (5' > 3')
| |
- | |GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTAGCGTTCATAATTTCGCTCAG
| |
- | |- style="background: #cbcee0;"
| |
- | |style="background:#0d3e99; color:white; text-align: right;"|Designer
| |
- | |G. Cambray
| |
- | |- style="background: #cbcee0;"
| |
- | !colspan="6" style="background:#c5c5c5; text-align: right;" | Not Tested
| |
- | |}
| |
- |
| |
- |
| |
- |
| |
- | {|
| |
- | ! colspan="6" style="background: #c5c5c5;" | [O17] Tg3p_Rw
| |
- | |- style="background: #cbcee0;"
| |
- | |style="background:#0d3e99; color:white; text-align: right;" width=120px|Description
| |
- | |width=700px| Amplification of N-term domain of M13's g3p
| |
- | |- style="background: #cbcee0;"
| |
- | |style="background:#0d3e99; color:white; text-align: right;"|Sequence (5' > 3')
| |
- | |GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTAACACTGAGTTTCGTCACCAGTAC
| |
- | |- style="background: #cbcee0;"
| |
- | |style="background:#0d3e99; color:white; text-align: right;"|Designer
| |
- | |G. Cambray
| |
- | |- style="background: #cbcee0;"
| |
- | !colspan="6" style="background:#c5c5c5; text-align: right;" | Not Tested
| |
- | |}
| |
- |
| |
- |
| |
- | {|
| |
- | ! colspan="6" style="background: #c5c5c5;" | [O18] Tg3p_fusion_Fw
| |
- | |- style="background: #cbcee0;"
| |
- | |style="background:#0d3e99; color:white; text-align: right;" width=120px|Description
| |
- | |width=700px| Fusion of N-term domain of M13's g3p (standard 23)
| |
- | |- style="background: #cbcee0;"
| |
- | |style="background:#0d3e99; color:white; text-align: right;"|Sequence (5' > 3')
| |
- | |GTTTCTTCGAATTCGCGGCCGCTTCTAGAAAAAAATTATTATTCGCAATTCCTTTAG
| |
- | |- style="background: #cbcee0;"
| |
- | |style="background:#0d3e99; color:white; text-align: right;"|Designer
| |
- | |G. Cambray
| |
- | |- style="background: #cbcee0;"
| |
- | !colspan="6" style="background:#c5c5c5; text-align: right;" | Not Tested
| |
- | |}
| |
- |
| |
- |
| |
- | {|
| |
- | ! colspan="6" style="background: #c5c5c5;" | [O19] g3p_F
| |
- | |- style="background: #cbcee0;"
| |
- | |style="background:#0d3e99; color:white; text-align: right;" width=120px|Description
| |
- | |width=700px| g3p
| |
- | |- style="background: #cbcee0;"
| |
- | |style="background:#0d3e99; color:white; text-align: right;"|Sequence (5' > 3')
| |
- | |GTTTCTTCGAATTCGCGGCCGCTTCTAGAGCTCCGCTGAAACTGTTGAAAGTTG
| |
- | |- style="background: #cbcee0;"
| |
- | |style="background:#0d3e99; color:white; text-align: right;"|Designer
| |
- | |G. Cambray
| |
- | |- style="background: #cbcee0;"
| |
- | !colspan="6" style="background:#c5c5c5; text-align: right;" | Not Tested
| |
- | |}
| |
- |
| |
- |
| |
- | {|
| |
- | ! colspan="6" style="background: #c5c5c5;" | [O20] g3p_R
| |
- | |- style="background: #cbcee0;"
| |
- | |style="background:#0d3e99; color:white; text-align: right;" width=120px|Description
| |
- | |width=700px| g3p
| |
- | |- style="background: #cbcee0;"
| |
- | |style="background:#0d3e99; color:white; text-align: right;"|Sequence (5' > 3')
| |
- | |GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTAAGACTCCTTATTACGCAG
| |
- | |- style="background: #cbcee0;"
| |
- | |style="background:#0d3e99; color:white; text-align: right;"|Designer
| |
- | |G. Cambray
| |
- | |- style="background: #cbcee0;"
| |
- | !colspan="6" style="background:#c5c5c5; text-align: right;" | Not Tested
| |
- | |}
| |
- |
| |
- |
| |
- | {|
| |
- | ! colspan="6" style="background: #c5c5c5;" | [O21] Ompa_linker_F
| |
- | |- style="background: #cbcee0;"
| |
- | |style="background:#0d3e99; color:white; text-align: right;" width=120px|Description
| |
- | |width=700px| For standardisation of OmpA linker (BBaK103006)
| |
- | |- style="background: #cbcee0;"
| |
- | |style="background:#0d3e99; color:white; text-align: right;"|Sequence (5' > 3')
| |
- | |TTGAATTCGCGGCCGCTTCTAGAAGGAGGTATATAATGAAAGCTACTAAACTGGTACTGG
| |
- | |- style="background: #cbcee0;"
| |
- | |style="background:#0d3e99; color:white; text-align: right;"|Designer
| |
- | |G. Cambray
| |
- | |- style="background: #cbcee0;"
| |
- | !colspan="6" style="background:#c5c5c5; text-align: right;" | Not Tested
| |
- | |}
| |
- |
| |
- |
| |
- | {|
| |
- | ! colspan="6" style="background: #c5c5c5;" | [O22] Ompa_linker_R
| |
- | |- style="background: #cbcee0;"
| |
- | |style="background:#0d3e99; color:white; text-align: right;" width=120px|Description
| |
- | |width=700px| For standardisation of OmpA linker (BBaK103006)
| |
- | |- style="background: #cbcee0;"
| |
- | |style="background:#0d3e99; color:white; text-align: right;"|Sequence (5' > 3')
| |
- | |GTTTCTTCCTGCAGCGGCCGCTACTAGTAGAGCTCCCTCCTCCAGAACC
| |
- | |- style="background: #cbcee0;"
| |
- | |style="background:#0d3e99; color:white; text-align: right;"|Designer
| |
- | |G. Cambray
| |
- | |- style="background: #cbcee0;"
| |
- | !colspan="6" style="background:#c5c5c5; text-align: right;" | Not Tested
| |
- | |}
| |
- |
| |
- |
| |
- | {|
| |
- | ! colspan="6" style="background: #c5c5c5;" | [O23] OmpAsignal-TE3_seq_Fw
| |
- | |- style="background: #cbcee0;"
| |
- | |style="background:#0d3e99; color:white; text-align: right;" width=120px|Description
| |
- | |width=700px| ???
| |
- | |- style="background: #cbcee0;"
| |
- | |style="background:#0d3e99; color:white; text-align: right;"|Sequence (5' > 3')
| |
- | |CACAAATAGCGAAAGATGAC
| |
- | |- style="background: #cbcee0;"
| |
- | |style="background:#0d3e99; color:white; text-align: right;"|Designer
| |
- | |G. Cambray
| |
- | |- style="background: #cbcee0;"
| |
- | !colspan="6" style="background:#c5c5c5; text-align: right;" | Not Tested
| |
- | |}
| |
- |
| |
- |
| |
- | {|
| |
- | ! colspan="6" style="background: #c5c5c5;" | [O24] OmpAsignal-TE3_seq_Rw
| |
- | |- style="background: #cbcee0;"
| |
- | |style="background:#0d3e99; color:white; text-align: right;" width=120px|Description
| |
- | |width=700px| ???
| |
- | |- style="background: #cbcee0;"
| |
- | |style="background:#0d3e99; color:white; text-align: right;"|Sequence (5' > 3')
| |
- | |ATCATCTGCGGGTAATGATG
| |
- | |- style="background: #cbcee0;"
| |
- | |style="background:#0d3e99; color:white; text-align: right;"|Designer
| |
- | |G. Cambray
| |
- | |- style="background: #cbcee0;"
| |
- | !colspan="6" style="background:#c5c5c5; text-align: right;" | Not Tested
| |
- | |}
| |
- |
| |
- |
| |
- | {|
| |
- | ! colspan="6" style="background: #c5c5c5;" | [O25] VAMP2_Seq_Fw
| |
- | |- style="background: #cbcee0;"
| |
- | |style="background:#0d3e99; color:white; text-align: right;" width=120px|Description
| |
- | |width=700px| ???
| |
- | |- style="background: #cbcee0;"
| |
- | |style="background:#0d3e99; color:white; text-align: right;"|Sequence (5' > 3')
| |
- | |ACTCACTATAGGGAGACCACA
| |
- | |- style="background: #cbcee0;"
| |
- | |style="background:#0d3e99; color:white; text-align: right;"|Designer
| |
- | |G. Cambray
| |
- | |- style="background: #cbcee0;"
| |
- | !colspan="6" style="background:#c5c5c5; text-align: right;" | Not Tested
| |
- | |}
| |
- |
| |
- |
| |
- | {|
| |
- | ! colspan="6" style="background: #c5c5c5;" | [O26] VAMP2_Seq_Rv
| |
- | |- style="background: #cbcee0;"
| |
- | |style="background:#0d3e99; color:white; text-align: right;" width=120px|Description
| |
- | |width=700px| ???
| |
- | |- style="background: #cbcee0;"
| |
- | |style="background:#0d3e99; color:white; text-align: right;"|Sequence (5' > 3')
| |
- | |GGGCTTTGTTAGCAGCCGGAT
| |
- | |- style="background: #cbcee0;"
| |
- | |style="background:#0d3e99; color:white; text-align: right;"|Designer
| |
- | |G. Cambray
| |
- | |- style="background: #cbcee0;"
| |
- | !colspan="6" style="background:#c5c5c5; text-align: right;" | Not Tested
| |
- | |}
| |
- |
| |
- |
| |
- | {|
| |
- | ! colspan="6" style="background: #c5c5c5;" | [O27] ClyA_seq_Fw
| |
- | |- style="background: #cbcee0;"
| |
- | |style="background:#0d3e99; color:white; text-align: right;" width=120px|Description
| |
- | |width=700px| ???
| |
- | |- style="background: #cbcee0;"
| |
- | |style="background:#0d3e99; color:white; text-align: right;"|Sequence (5' > 3')
| |
- | |GCAGCTATTTCCAGTCACAG
| |
- | |- style="background: #cbcee0;"
| |
- | |style="background:#0d3e99; color:white; text-align: right;"|Designer
| |
- | |G. Cambray
| |
- | |- style="background: #cbcee0;"
| |
- | !colspan="6" style="background:#c5c5c5; text-align: right;" | Not Tested
| |
- | |}
| |
- |
| |
- |
| |
- | {|
| |
- | ! colspan="6" style="background: #c5c5c5;" | [O28] ClyA_seq_Rw
| |
- | |- style="background: #cbcee0;"
| |
- | |style="background:#0d3e99; color:white; text-align: right;" width=120px|Description
| |
- | |width=700px| ???
| |
- | |- style="background: #cbcee0;"
| |
- | |style="background:#0d3e99; color:white; text-align: right;"|Sequence (5' > 3')
| |
- | |CGCCCGCAGCAATAGAATAG
| |
- | |- style="background: #cbcee0;"
| |
- | |style="background:#0d3e99; color:white; text-align: right;"|Designer
| |
- | |G. Cambray
| |
- | |- style="background: #cbcee0;"
| |
- | !colspan="6" style="background:#c5c5c5; text-align: right;" | Not Tested
| |
- | |}
| |
- |
| |
- |
| |
- | {|
| |
- | ! colspan="6" style="background: #c5c5c5;" | [O29] PFecA_Fw
| |
- | |- style="background: #cbcee0;"
| |
- | |style="background:#0d3e99; color:white; text-align: right;" width=120px|Description
| |
- | |width=700px| ???
| |
- | |- style="background: #cbcee0;"
| |
- | |style="background:#0d3e99; color:white; text-align: right;"|Sequence (5' > 3')
| |
- | |GTTTCTTCGAATTCGCGGCCGCTTCTAGAGTACGCGGTACTGGATAAACATTTC
| |
- | |- style="background: #cbcee0;"
| |
- | |style="background:#0d3e99; color:white; text-align: right;"|Designer
| |
- | |G. Cambray
| |
- | |- style="background: #cbcee0;"
| |
- | !colspan="6" style="background:#c5c5c5; text-align: right;" | Not Tested
| |
- | |}
| |
- |
| |
- |
| |
- | {|
| |
- | ! colspan="6" style="background: #c5c5c5;" | [O30] PFecA_Rv
| |
- | |- style="background: #cbcee0;"
| |
- | |style="background:#0d3e99; color:white; text-align: right;" width=120px|Description
| |
- | |width=700px| ???
| |
- | |- style="background: #cbcee0;"
| |
- | |style="background:#0d3e99; color:white; text-align: right;"|Sequence (5' > 3')
| |
- | |GTTTCTTCCTGCAGCGGCCGCTACTAGTATTTTTGTTGTGTTCAGCTATGAGT
| |
- | |- style="background: #cbcee0;"
| |
- | |style="background:#0d3e99; color:white; text-align: right;"|Designer
| |
- | |G. Cambray
| |
- | |- style="background: #cbcee0;"
| |
- | !colspan="6" style="background:#c5c5c5; text-align: right;" | Not Tested
| |
- | |}
| |
- |
| |
- |
| |
- | <html>
| |
- | </div>
| |
- | <div id="paris_content_boxtop">
| |
- | </div>
| |
- | <div id="paris_content">
| |
| </html> | | </html> |
| | | |
- | == PCR products ==
| |
- |
| |
- | {|
| |
- | |colspan="11" style="background: #660099;"|PCR products
| |
- | |- style="background: #0d3e99; text-align: center; color:white;"
| |
- | |width=5%| Number
| |
- | |width=10%| Name
| |
- | |width=10%| Oligo F
| |
- | |width=10%| Oligo R
| |
- | |width=5%| Tm
| |
- | |width=10%| Matrix
| |
- | |width=10%| Length
| |
- | |width=25%| Comments
| |
- | |width=5%| Quantity
| |
- | |width=5%| []ng/µL
| |
- | |width=5%| Date
| |
- | |- style="background: #ccccff; text-align: center;"
| |
- | |width=5%| A1
| |
- | |width=10%| g3p_std
| |
- | |width=10%| [O19] g3p_F
| |
- | |width=10%| [O20] g3p_R
| |
- | |width=5%| Tm
| |
- | |width=10%| Matrix
| |
- | |width=10%| Length
| |
- | |width=25%| For standardisation of g3p and adding SacI site
| |
- | |width=5%| ?
| |
- | |width=5%| ? ng/µL
| |
- | |width=5%| ?
| |
- | |- style="background: #ccccff; text-align: center;"
| |
- | |width=5%| A2
| |
- | |width=10%| OmpA_L_std
| |
- | |width=10%| [O21] Ompa_linker_F
| |
- | |width=10%| [O22] Ompa_linker_R
| |
- | |width=5%| Tm
| |
- | |width=10%| Matrix
| |
- | |width=10%| Length
| |
- | |width=25%| For standardisation of OmpA linker (BBaK103006)
| |
- | |width=5%| ?
| |
- | |width=5%| ? ng/µL
| |
- | |width=5%| ?
| |
- | |}
| |
| <br> | | <br> |
- | <br>
| |
- | http://parts.mit.edu/r/parts/images/partbypart/icon_terminator.png
| |
- | http://parts.mit.edu/images/partbypart/icon_regulatory.png
| |
- | http://parts.mit.edu/r/parts/images/partbypart/icon_rbs.png
| |
- | http://parts.mit.edu/r/parts/images/partbypart/icon_reporter.png
| |
- | http://parts.mit.edu/registry/images/7/7b/Part_icon_cds.png
| |
- | http://parts.mit.edu/registry/images/9/9f/Part_icon_dna.png
| |
- | http://parts.mit.edu/registry/images/2/2e/Part_icon_generator.png
| |
- | http://parts.mit.edu/registry/images/a/ab/Part_icon_plasmid.png
| |
- |
| |
- | <html>
| |
- | </div>
| |
- | <div id="paris_content_boxtop">
| |
- | </div>
| |
- | <div id="paris_content">
| |
- | </html>
| |
- |
| |
- | == Plasmids: MiniPrep Products ==
| |
- | {|
| |
- | |- style="background: #c5c5c5;"
| |
- | ! colspan="5" style="background: #c5c5c5;" | Plasmids: MiniPrep Products
| |
- | |- style="background: #0d3e99; text-align: center; color:white;"
| |
- | |width=5%| Number
| |
- | |width=25%| Name
| |
- | |width=50%| Description
| |
- | |width=15%| Products in use
| |
- | |width=5%| Date
| |
- | |- style="background: #ccccff; text-align: center;"
| |
- | |width=5%| P1
| |
- | |width=25%| pSB2K3
| |
- | |width=50%| General plasmid for cloning step after PCR
| |
- | |width=15%| Products in use
| |
- | |width=5%| [https://2009.igem.org/Team:Paris/3_August_2009 08/03]
| |
- | |- style="background: #ccccff; text-align: center;"
| |
- | |width=5%| P2
| |
- | |width=25%| Bba-J61002
| |
- | |width=50%| Plasmid backbone, it allows us to change promoter RFP as promoter
| |
- | |width=15%| Products in use
| |
- | |width=5%| [https://2009.igem.org/Team:Paris/3_August_2009 08/03]
| |
- | |- style="background: #ccccff; text-align: center;"
| |
- | |width=5%| P3
| |
- | |width=25%| Bba-J32015
| |
- | |width=50%| PelB leader sequence
| |
- | |width=15%| Products in use
| |
- | |width=5%| [https://2009.igem.org/Team:Paris/3_August_2009 08/03]
| |
- | |- style="background: #ccccff; text-align: center;"
| |
- | |width=5%| P4
| |
- | |width=25%| Bba-B0014
| |
- | |width=50%| Double terminator bidirectionnal (B0012-B0011)
| |
- | |width=15%| Products in use
| |
- | |width=5%| [https://2009.igem.org/Team:Paris/3_August_2009 08/03]
| |
- | |- style="background: #ccccff; text-align: center;"
| |
- | |width=5%| P5
| |
- | |width=25%| Bba-I14032
| |
- | |width=50%| pLac
| |
- | |width=15%| Products in use
| |
- | |width=5%| [https://2009.igem.org/Team:Paris/3_August_2009 08/03]
| |
- | |- style="background: #ccccff; text-align: center;"
| |
- | |width=5%| P6
| |
- | |width=25%| BBa_K103006
| |
- | |width=50%| OmpA linker (464bp)
| |
- | |width=15%| Products in use
| |
- | |width=5%| [https://2009.igem.org/Team:Paris/3_August_2009 08/03]
| |
- | |- style="background: #ccccff; text-align: center;"
| |
- | |width=5%| P7
| |
- | |width=25%| Bba-R0040
| |
- | |width=50%| TetR repressible promoter
| |
- | |width=15%| Products in use
| |
- | |width=5%| [https://2009.igem.org/Team:Paris/3_August_2009 08/03]
| |
- | |- style="background: #ccccff; text-align: center;"
| |
- | |width=5%| P8
| |
- | |width=25%| Bba-I0500
| |
- | |width=50%| pBad/araC
| |
- | |width=15%| Products in use
| |
- | |width=5%| [https://2009.igem.org/Team:Paris/3_August_2009 08/03]
| |
- | |- style="background: #ccccff; text-align: center;"
| |
- | |width=5%| P9
| |
- | |width=25%| ΔN-Syn1A
| |
- | |width=50%| ???
| |
- | |width=15%| Products in use
| |
- | |width=5%| [https://2009.igem.org/Team:Paris/3_August_2009 08/03]
| |
- | |- style="background: #ccccff; text-align: center;"
| |
- | |width=5%| P10
| |
- | |width=25%| His6-SNAP25
| |
- | |width=50%| ???
| |
- | |width=15%| Products in use
| |
- | |width=5%| [https://2009.igem.org/Team:Paris/3_August_2009 08/03]
| |
- | |- style="background: #ccccff; text-align: center;"
| |
- | |width=5%| P11
| |
- | |width=25%| VAMP2-HiB6
| |
- | |width=50%| ???
| |
- | |width=15%| Products in use
| |
- | |width=5%| [https://2009.igem.org/Team:Paris/3_August_2009 08/03]
| |
- | |- style="background: #ccccff; text-align: center;"
| |
- | |width=5%| P12
| |
- | |width=25%| g3p
| |
- | |width=50%| g3p in pHEN2 plasmid
| |
- | |width=15%| Products in use
| |
- | |width=5%| [https://2009.igem.org/Team:Paris/4_August_2009 08/04]
| |
- | |}
| |
- | <html>
| |
- | </div>
| |
- | <div id="paris_content_boxtop">
| |
- | </div>
| |
- | <div id="paris_content">
| |
- | </html>
| |
- |
| |
- | == Digestion Products ==
| |
- | <div style="background-color:#FF0000; padding:1px; margin:1px; font-size:120%; text-align:center; font-weight:bold; border:1px solid black">Red = Vector</div>
| |
- | <div style="background-color:#FFFF00; padding:1px; margin:1px; font-size:120%; text-align:center; font-weight:bold; border:1px solid black">Yellow = Insert</div>
| |
- |
| |
- | {|
| |
- | |- style="background: #c5c5c5;"
| |
- | ! colspan="10" style="background: #c5c5c5;" | Digestion Products
| |
- | |- style="background: #0d3e99; text-align: center; color:white;"
| |
- | |width=5%| Number
| |
- | |width=20%| Product Name
| |
- | |width=20%| Matrix Name
| |
- | |width=5%| Enz. 1
| |
- | |width=5%| Enz. 2
| |
- | |width=5%| Size
| |
- | |width=20%| Description
| |
- | |width=10%| Product Left
| |
- | |width=5%| []ng/µL
| |
- | |width=5%| Date
| |
- | |- style="background: #ccccff; text-align: center;"
| |
- | |style="background:#FFFF00"| D1
| |
- | | P3
| |
- | | BBa-J32015
| |
- | | XbaI
| |
- | | PstI
| |
- | | Size
| |
- | | ???
| |
- | | ???
| |
- | | ???
| |
- | | [https://2009.igem.org/Team:Paris/4_August_2009 08/04]
| |
- | |- style="background: #ccccff; text-align: center;"
| |
- | |style="background:#FFFF00"| D2
| |
- | | P4
| |
- | | BBa-B0014
| |
- | | XbaI
| |
- | | PstI
| |
- | | Size
| |
- | | ???
| |
- | | ???
| |
- | | ???
| |
- | | [https://2009.igem.org/Team:Paris/4_August_2009 08/04]
| |
- | |- style="background: #ccccff; text-align: center;"
| |
- | |style="background:#FFFF00"| D3
| |
- | | P5
| |
- | | BBa-I14032
| |
- | | XbaI
| |
- | | PstI
| |
- | | Size
| |
- | | ???
| |
- | | ???
| |
- | | ???
| |
- | | [https://2009.igem.org/Team:Paris/4_August_2009 08/04]
| |
- | |- style="background: #ccccff; text-align: center;"
| |
- | |style="background:#FFFF00"| D4
| |
- | | P7
| |
- | | Bba-R0040
| |
- | | XbaI
| |
- | | PstI
| |
- | | Size
| |
- | | ???
| |
- | | ???
| |
- | | ???
| |
- | | [https://2009.igem.org/Team:Paris/4_August_2009 08/04]
| |
- | |- style="background: #ccccff; text-align: center;"
| |
- | |style="background:#FFFF00"| D5
| |
- | | P8
| |
- | | BBa-10500
| |
- | | XbaI
| |
- | | PstI
| |
- | | Size
| |
- | | ???
| |
- | | ???
| |
- | | ???
| |
- | | [https://2009.igem.org/Team:Paris/4_August_2009 08/04]
| |
- | |- style="background: #ccccff; text-align: center;"
| |
- | |style="background:#FFFF00"| D6
| |
- | | ???
| |
- | | ???
| |
- | | XbaI
| |
- | | PstI
| |
- | | Size
| |
- | | ???
| |
- | | ???
| |
- | | ???
| |
- | | [https://2009.igem.org/Team:Paris/4_August_2009 08/04]
| |
- | |- style="background: #ccccff; text-align: center;"
| |
- | |style="background:#FFFF00"| D7
| |
- | | ???
| |
- | | ???
| |
- | | XbaI
| |
- | | PstI
| |
- | | Size
| |
- | | ???
| |
- | | ???
| |
- | | ???
| |
- | | [https://2009.igem.org/Team:Paris/4_August_2009 08/04]
| |
- | |}
| |
- | <html>
| |
- | </div>
| |
- | <div id="paris_content_boxtop">
| |
- | </div>
| |
- | <div id="paris_content">
| |
- | </html>
| |
- |
| |
- | == Unexpected digestion results ==
| |
- | {|
| |
- | |- style="background: #c5c5c5;"
| |
- | ! colspan="7" style="background: #c5c5c5;" | Digestion Products
| |
- | |- style="background: #0d3e99; text-align: center; color:white;"
| |
- | |width=5%| Number
| |
- | |width=30%| Matrix Name
| |
- | |width=5%| Enz. 1
| |
- | |width=5%| Enz. 2
| |
- | |width=10%| Expected Size
| |
- | |width=40%| Description
| |
- | |width=5%| Date
| |
- | |}
| |
- |
| |
- |
| |
- | <html>
| |
- | </div>
| |
- | <div id="paris_content_boxtop">
| |
- | </div>
| |
- | <div id="paris_content">
| |
- | </html>
| |
- |
| |
- | == Strains (Glycerol stock) ==
| |
| {| | | {| |
| |- style="background: #c5c5c5;" | | |- style="background: #c5c5c5;" |
- | ! colspan="5" style="background: #c5c5c5;" | Strains glycerol stock | + | ! colspan="8" style="background:#c5c5c5; text-align: center" | Color code for sticker |
- | |- style="background: #0d3e99; text-align: center; color:white;"
| + | |- style="background: #0d3e99; text-align: center; color:black;" |
- | |width=5%| Number | + | |width=10% style="background: #FF0000;"| Vector |
- | |Width=2%|R
| + | |width=10% style="background: #FFFF00;"| Insert |
- | |width=25%| Name
| + | |width=10% style="background: #660099;"| PCR product |
- | |width=55%| Description
| + | |width=10% style="background: #FF6600;"| Plasmid |
- | |width=5%| Date
| + | |width=10% style="background: #FFFFFF;"| Strains |
- | |- style="background: #cbcee0; text-align: center;"
| + | |width=10% style="background: #006400;"| Kanamycin |
- | |<strike>[https://2009.igem.org/Team:Paris/Strains#Strain_1 S1]</strike>
| + | |width=10% style="background: #3333FF;"| Ampicilin |
- | |style="background:#cccccc;"|
| + | |width=10% style="background: #FF0066;"| Chloranphenicol |
- | |<strike>DH5α</strike>
| + | |
- | |<strike>DH5α E. Coli strain for chimically competent</strike>
| + | |
- | |[https://2009.igem.org/Team:Paris/20_July_2009 07/20]
| + | |
- | |- style="background: #cbcee0; text-align: center;" | + | |
- | |[https://2009.igem.org/Team:Paris/Strains#Strain_2 S2]
| + | |
- | |style="background:#006400;"|K
| + | |
- | |K12 MG 1655 ΔrecA::Kan | + | |
- | |Standard E.Coli strain, Kan resistance
| + | |
- | |[https://2009.igem.org/Team:Paris/20_July_2009 07/20]
| + | |
- | |- style="background: #cbcee0; text-align: center;"
| + | |
- | |[https://2009.igem.org/Team:Paris/Strains#Strain_3 S3]
| + | |
- | |style="background:#006400;"|K
| + | |
- | |W3110 Δnlp1 | + | |
- | |From Daniel Rodriguez, Kan resistance
| + | |
- | |[https://2009.igem.org/Team:Paris/23_July_2009 07/23]
| + | |
- | |- style="background: #cbcee0; text-align: center;"
| + | |
- | |[https://2009.igem.org/Team:Paris/Strains#TOP10 TOP10]
| + | |
- | |style="background:#cccccc;"|
| + | |
- | |TOP10 | + | |
- | |Competent bacteria
| + | |
- | |[https://2009.igem.org/Team:Paris/27_July_2009 07/27]
| + | |
- | |- style="background: #cbcee0; text-align: center;"
| + | |
- | |[https://2009.igem.org/Team:Paris/Strains#Strain_4 S4]
| + | |
- | |style="background:#cccccc;"|
| + | |
- | |TB28 | + | |
- | |No kanamycin resistance
| + | |
- | |[https://2009.igem.org/Team:Paris/29_July_2009 07/29]
| + | |
- | |- style="background: #cbcee0; text-align: center;"
| + | |
- | |[https://2009.igem.org/Team:Paris/Strains#Strain_5 S5]
| + | |
- | |style="background:#006400;"|K
| + | |
- | |MG4 | + | |
- | |Strain needs to be grown on media with [NaCl]>=0.5%, preferably 1%NaCl
| + | |
- | |[https://2009.igem.org/Team:Paris/29_July_2009 07/29]
| + | |
- | |- style="background: #cbcee0; text-align: center;"
| + | |
- | |[https://2009.igem.org/Team:Paris/Strains#Strain_6 S6] | + | |
- | |style="background:#cccccc;"| | + | |
- | |DH5α
| + | |
- | |DH5α E. Coli strain for chimically competent (Pasteur)
| + | |
- | |[https://2009.igem.org/Team:Paris/30_July_2009 07/30]
| + | |
- | |- style="background: #cbcee0; text-align: center;"
| + | |
- | |[https://2009.igem.org/Team:Paris/Strains#Strain_7 S7]
| + | |
- | |style="background:#3333FF;"|A
| + | |
- | |DH5α(g3p)
| + | |
- | |DH5α E. Coli strain with g3p in plasmid, Amp resistance
| + | |
- | |[https://2009.igem.org/Team:Paris/30_July_2009 07/30]
| + | |
- | |- style="background: #cbcee0; text-align: center;"
| + | |
- | |[https://2009.igem.org/Team:Paris/Strains#Strain_8 S8]
| + | |
- | |style="background:#006400;"|K
| + | |
- | |DH5α(pSB2K3) | + | |
- | |DH5α E. Coli strain with pSB2K3 plasmid, Kan resistance
| + | |
- | |[https://2009.igem.org/Team:Paris/3_August_2009 08/03]
| + | |
- | |- style="background: #cbcee0; text-align: center;"
| + | |
- | |[https://2009.igem.org/Team:Paris/Strains#Strain_9 S9]
| + | |
- | |style="background:#3333FF;"|A
| + | |
- | |DH5α(BBa_J61002) | + | |
- | |DH5α E. Coli strain with BBa_J61002 in plasmid, Amp resistance
| + | |
- | |[https://2009.igem.org/Team:Paris/3_August_2009 08/03]
| + | |
- | |- style="background: #cbcee0; text-align: center;"
| + | |
- | |[https://2009.igem.org/Team:Paris/Strains#Strain_10 S10]
| + | |
- | |style="background:#006400;"|K
| + | |
- | |DH5α(BBa_J32015)
| + | |
- | |DH5α E. Coli strain with BBa_J32015 in plasmid, Kan resistance
| + | |
- | |[https://2009.igem.org/Team:Paris/3_August_2009 08/03]
| + | |
- | |- style="background: #cbcee0; text-align: center;"
| + | |
- | |[https://2009.igem.org/Team:Paris/Strains#Strain_11 S11]
| + | |
- | |style="background:#006400;"|K
| + | |
- | |DH5α(BBa_B0014)
| + | |
- | |DH5α E. Coli strain with BBa_B0014 in plasmid, Kan resistance
| + | |
- | |[https://2009.igem.org/Team:Paris/3_August_2009 08/03]
| + | |
- | |- style="background: #cbcee0; text-align: center;"
| + | |
- | |[https://2009.igem.org/Team:Paris/Strains#Strain_12 S12]
| + | |
- | |style="background:#006400;"|K
| + | |
- | |DH5α(BBa_I14032)
| + | |
- | |DH5α E. Coli strain with BBa_I14032 in plasmid, Kan resistance
| + | |
- | |[https://2009.igem.org/Team:Paris/3_August_2009 08/03]
| + | |
- | |- style="background: #cbcee0; text-align: center;"
| + | |
- | |[https://2009.igem.org/Team:Paris/Strains#Strain_13 S13]
| + | |
- | |style="background:#006400;"|K
| + | |
- | |DH5α(BBa_K103006)
| + | |
- | |DH5α E. Coli strain with BBa_K103006 in plasmid, Kan resistance
| + | |
- | |[https://2009.igem.org/Team:Paris/3_August_2009 08/03]
| + | |
- | |- style="background: #cbcee0; text-align: center;"
| + | |
- | |[https://2009.igem.org/Team:Paris/Strains#Strain_14 S14]
| + | |
- | |style="background:#3333FF;"|A
| + | |
- | |DH5α(BBa_R0040)
| + | |
- | |DH5α E. Coli strain with BBa_R0040 in plasmid, Amp resistance
| + | |
- | |[https://2009.igem.org/Team:Paris/3_August_2009 08/03]
| + | |
- | |- style="background: #cbcee0; text-align: center;"
| + | |
- | |[https://2009.igem.org/Team:Paris/Strains#Strain_15 S15]
| + | |
- | |style="background:#006400;"|K
| + | |
- | |DH5α(BBa_10500)
| + | |
- | |DH5α E. Coli strain with BBa_10500 in plasmid, Kan resistance
| + | |
- | |[https://2009.igem.org/Team:Paris/3_August_2009 08/03]
| + | |
- | |- style="background: #cbcee0; text-align: center;"
| + | |
- | |[https://2009.igem.org/Team:Paris/Strains#Strain_16 S16]
| + | |
- | |style="background:#006400;"|K
| + | |
- | |DH5α(ΔN-Syn1A)
| + | |
- | |DH5α E. Coli strain with ΔN-Syn1A in plasmid, Kan resistance
| + | |
- | |[https://2009.igem.org/Team:Paris/3_August_2009 08/03]
| + | |
- | |- style="background: #cbcee0; text-align: center;"
| + | |
- | |[https://2009.igem.org/Team:Paris/Strains#Strain_17 S17]
| + | |
- | |style="background:#3333FF;"|A
| + | |
- | |DH5α(His6-SNAP25)
| + | |
- | |DH5α E. Coli strain with His6-SNAP25 in plasmid, Amp resistance
| + | |
- | |[https://2009.igem.org/Team:Paris/3_August_2009 08/03]
| + | |
- | |- style="background: #cbcee0; text-align: center;"
| + | |
- | |[https://2009.igem.org/Team:Paris/Strains#Strain_18 S18]
| + | |
- | |style="background:#3333FF;"|A
| + | |
- | |DH5α(Vamp2-His6)
| + | |
- | |DH5α E. Coli strain with Vamp2-His6 in plasmid, Amp resistance
| + | |
- | |[https://2009.igem.org/Team:Paris/3_August_2009 08/03]
| + | |
- | |- style="background: #cbcee0; text-align: center;"
| + | |
- | |[https://2009.igem.org/Team:Paris/Strains#Strain_19 S19]
| + | |
- | |style="background:#3333FF;"|A
| + | |
- | |DH5α(puc19)
| + | |
- | |DH5α E. Coli strain with puc19 plasmid, Amp resistance
| + | |
- | |[https://2009.igem.org/Team:Paris/30_July_2009 07/30]
| + | |
| |} | | |} |