Team:Paris/Freezer

From 2009.igem.org

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<span/ id="bottom">[https://2009.igem.org/ iGEM ] > [[Team:Paris#top | Paris]] > [[Team:Paris/Freezer#top | Freezer]] > [[Team:Paris/Freezer#bottom | Main]]
{{Template:Paris2009}}
{{Template:Paris2009}}
{{Template:Paris2009_menu}}
{{Template:Paris2009_menu}}
 +
==Freezer==
==Freezer==
-
*[[Team:Paris/Freezer#Primers | Primers]]
 
-
*[[Team:Paris/Freezer#PCR_products | PCR products]]
 
-
*[[Team:Paris/Freezer#Plasmids:_MiniPrep_Products | Plasmids: MiniPrep Products]]
 
-
*[[Team:Paris/Freezer#Digestion_Products | Digestion Products]]
 
-
*[[Team:Paris/Freezer#Unexpected_digestion_results | Unexpected digestion results]]
 
-
*[[Team:Paris/Freezer#Strains_(Glycerol stock) | Strains (Glycerol stock)]]
 
-
 
-
 
-
*Color code for sticker
 
-
**<span style="background-color:#FF0000">Vector</span>
 
-
**<span style="background-color:#FFFF00">Insert</span>
 
-
**<span style="background-color:#660099">PCR product</span>
 
-
**<span style="background-color:#FF6600">Plasmid</span>
 
-
**<span style="background-color:#006400">Kanamycin</span>
 
-
**<span style="background-color:#3333FF">Ampicilin</span>
 
-
**<span style="background-color:#FF0066">Chloranphenicol</span>
 
<html>
<html>
-
</div>
+
<style type="text/css">
-
<div id="paris_content_boxtop">
+
#left-side {
-
</div>
+
    position: absolute;
-
<div id="paris_content">
+
    height: 23px;
-
</html>
+
    width: 30px;
 +
    top: 0px;
 +
    left: 10px;
 +
    margin-top:10px;
 +
    padding-top: 7px;
 +
    background: url(https://static.igem.org/mediawiki/2009/1/1b/Left_menu_pari.png);
 +
    z-index:4;
 +
}
-
== Primers ==
+
#middle-side {
-
{|
+
    height: 25px;
-
! colspan="6" style="background: #c5c5c5;" | [O1] Rev P1
+
    width: 650px;
-
|- style="background: #cbcee0;"
+
    position: absolute;
-
|style="background:#0d3e99; color:white; text-align: right;" width=120px|Description
+
    top: 0px;
-
|width=700px| tolR
+
    left: 30px;
-
|- style="background: #cbcee0;"
+
    margin-top:10px;
-
|style="background:#0d3e99; color:white; text-align: right;"|Sequence (5' > 3')
+
    padding-top: 5px;
-
|TCTGCTGCAGCGGCCGCTACTAGTATTAGTAAGGCACATCTTTTGCGCCACCG
+
    background: #dadada;
-
|- style="background: #cbcee0;"
+
    z-index:5;
-
|style="background:#0d3e99; color:white; text-align: right;"|Designer
+
}
-
|D. Bikard
+
-
|- style="background: #cbcee0;"
+
-
!colspan="6" style="background:#c5c5c5; text-align: right;" | Worked
+
-
|}
+
 +
#right-side {
 +
    position: absolute;
 +
    height: 23px;
 +
    width: 30px;
 +
    margin-top:10px;
 +
    padding-top: 7px;
 +
    top: 0px;
 +
    left: 670px;
 +
    background: url(https://static.igem.org/mediawiki/2009/4/40/Right_menu_paris.png);
 +
    z-index:4;
 +
}
-
{|
+
a.menu_sub {
-
! colspan="6" style="background: #c5c5c5;" | [O2] Fwd P2
+
    padding-left: 7px;
-
|- style="background: #cbcee0;"
+
    padding-right: 7px;
-
|style="background:#0d3e99; color:white; text-align: right;" width=120px|Description
+
}
-
|width=700px| tolR (DO NOT USE ERROR)
+
-
|- style="background: #cbcee0;"
+
-
|style="background:#0d3e99; color:white; text-align: right;"|Sequence (5' > 3')
+
-
|TCTGGAATTCGCGGCCGCTTCTAGATGAAATCAACATTGTACCGTTGCTGGACGTACTG
+
-
|- style="background: #cbcee0;"
+
-
|style="background:#0d3e99; color:white; text-align: right;"|Designer
+
-
|D. Bikard
+
-
|- style="background: #cbcee0;"
+
-
!colspan="6" style="background:#c5c5c5; text-align: right;" | Worked
+
-
|}
+
 +
a.menu_sub_active {
 +
    padding-left: 7px;
 +
    padding-right: 7px;
 +
    color:#b0310e;
 +
    font-weight:bold;
 +
}
 +
</style>
 +
<div id="left-side"></div>
 +
<div id="middle-side"><center>
 +
<a class="menu_sub_active"href="https://2009.igem.org/Team:Paris/Freezer#bottom"> Main </a>|
 +
<a class="menu_sub" href="https://2009.igem.org/Team:Paris/Freezer_PCR_products#bottom"> PCR Products</a>|
 +
<a class="menu_sub"href="https://2009.igem.org/Team:Paris/Freezer_Plasmids#bottom"> Plasmids</a>|
 +
<a class="menu_sub"href="https://2009.igem.org/Team:Paris/Freezer_Digestion_Products#bottom"> Digestion Products</a>|
 +
<a class="menu_sub"href="https://2009.igem.org/Team:Paris/Freezer_Strains_(Glycerol_stock)#bottom"> Strains (Glycerol stock)</a>|
 +
<a class="menu_sub"href="https://2009.igem.org/Team:Paris/constructions#bottom"> Constructions in progress</a>
 +
</center>
 +
</div>
-
{|
+
<div id="right-side"></div>
-
! colspan="6" style="background: #c5c5c5;" | [O3] Rev P3
+
-
|- style="background: #cbcee0;"
+
-
|style="background:#0d3e99; color:white; text-align: right;" width=120px|Description
+
-
|width=700px| tolA
+
-
|- style="background: #cbcee0;"
+
-
|style="background:#0d3e99; color:white; text-align: right;"|Sequence (5' > 3')
+
-
|TCTGCTGCAGCGGCCGCTACTAGTATTATTACGGTTTGAAGTCCAATGGCG
+
-
|- style="background: #cbcee0;"
+
-
|style="background:#0d3e99; color:white; text-align: right;"|Designer
+
-
|D. Bikard
+
-
|- style="background: #cbcee0;"
+
-
!colspan="6" style="background:#c5c5c5; text-align: right;" | Worked
+
-
|}
+
-
 
-
{|
 
-
! colspan="6" style="background: #c5c5c5;" | [O4] Rev P4
 
-
|- style="background: #cbcee0;"
 
-
|style="background:#0d3e99; color:white; text-align: right;" width=120px|Description
 
-
|width=700px| tolA (ERROR: Not designed for N-term fusion)
 
-
|- style="background: #cbcee0;"
 
-
|style="background:#0d3e99; color:white; text-align: right;"|Sequence (5' > 3')
 
-
|TCTGGAATTCGCGGCCGCTTCTAGAATGGGTAATACTAAAAACAATGGCGC
 
-
|- style="background: #cbcee0;"
 
-
|style="background:#0d3e99; color:white; text-align: right;"|Designer
 
-
|D. Bikard
 
-
|- style="background: #cbcee0;"
 
-
!colspan="6" style="background:#c5c5c5; text-align: right;" | Worked
 
-
|}
 
-
 
-
 
-
{|
 
-
! colspan="6" style="background: #c5c5c5;" | [O5] Rev P5
 
-
|- style="background: #cbcee0;"
 
-
|style="background:#0d3e99; color:white; text-align: right;" width=120px|Description
 
-
|width=700px| tatABC
 
-
|- style="background: #cbcee0;"
 
-
|style="background:#0d3e99; color:white; text-align: right;"|Sequence (5' > 3')
 
-
|TCTGCTGCAGCGGCCGCTACTAGTATTATTATTCTTCAGTTTTTTCGCTTTCTGC
 
-
|- style="background: #cbcee0;"
 
-
|style="background:#0d3e99; color:white; text-align: right;"|Designer
 
-
|D. Bikard
 
-
|- style="background: #cbcee0;"
 
-
!colspan="6" style="background:#c5c5c5; text-align: right;" | Worked
 
-
|}
 
-
 
-
 
-
{|
 
-
! colspan="6" style="background: #c5c5c5;" | [06] Fwd P6
 
-
|- style="background: #cbcee0;"
 
-
|style="background:#0d3e99; color:white; text-align: right;" width=120px|Description
 
-
|width=700px| tatABC (without RBS)
 
-
|- style="background: #cbcee0;"
 
-
|style="background:#0d3e99; color:white; text-align: right;"|Sequence (5' > 3')
 
-
|TCTGGAATTCGCGGCCGCTTCTAGAATGGGTGGTATCAGTATTTGG
 
-
|- style="background: #cbcee0;"
 
-
|style="background:#0d3e99; color:white; text-align: right;"|Designer
 
-
|D. Bikard
 
-
|- style="background: #cbcee0;"
 
-
!colspan="6" style="background:#c5c5c5; text-align: right;" | Worked
 
-
|}
 
-
 
-
 
-
 
-
{|
 
-
! colspan="6" style="background: #c5c5c5;" | [O7] Rev P7
 
-
|- style="background: #cbcee0;"
 
-
|style="background:#0d3e99; color:white; text-align: right;" width=120px|Description
 
-
|width=700px| clyA fusion
 
-
|- style="background: #cbcee0;"
 
-
|style="background:#0d3e99; color:white; text-align: right;"|Sequence (5' > 3')
 
-
|TTTCTGCAGCGGCCGCTACTAGTGACTTCAGGTACCTCAAAGAGTGTC
 
-
|- style="background: #cbcee0;"
 
-
|style="background:#0d3e99; color:white; text-align: right;"|Designer
 
-
|D. Bikard
 
-
|- style="background: #cbcee0;"
 
-
!colspan="6" style="background:#c5c5c5; text-align: right;" | Worked
 
-
|}
 
-
 
-
 
-
 
-
 
-
{|
 
-
! colspan="6" style="background: #c5c5c5;" | [O8] Fwd P8
 
-
|- style="background: #cbcee0;"
 
-
|style="background:#0d3e99; color:white; text-align: right;" width=120px|Description
 
-
|width=700px| clyA fusion (ERROR: No poly-Gly linker)
 
-
|- style="background: #cbcee0;"
 
-
|style="background:#0d3e99; color:white; text-align: right;"|Sequence (5' > 3')
 
-
|TTTGAATTCGCGGCCGCTTCTAGAATGACTGAAATCGTTGCAGATAAAA
 
-
|- style="background: #cbcee0;"
 
-
|style="background:#0d3e99; color:white; text-align: right;"|Designer
 
-
|D. Bikard
 
-
|- style="background: #cbcee0;"
 
-
!colspan="6" style="background:#c5c5c5; text-align: right;" | Worked
 
-
|}
 
-
 
-
 
-
{|
 
-
! colspan="6" style="background: #c5c5c5;" | [O9] clyA_Rw
 
-
|- style="background: #cbcee0;"
 
-
|style="background:#0d3e99; color:white; text-align: right;" width=120px|Description
 
-
|width=700px| clyA
 
-
|- style="background: #cbcee0;"
 
-
|style="background:#0d3e99; color:white; text-align: right;"|Sequence (5' > 3')
 
-
|GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTAGACTTCAGGTACCTCAAAGAG
 
-
|- style="background: #cbcee0;"
 
-
|style="background:#0d3e99; color:white; text-align: right;"|Designer
 
-
|G. Cambray
 
-
|- style="background: #cbcee0;"
 
-
!colspan="6" style="background:#c5c5c5; text-align: right;" | Not Tested
 
-
|}
 
-
 
-
 
-
 
-
{|
 
-
! colspan="6" style="background: #c5c5c5;" | [O10] clyA_fusion_polyG_Rw
 
-
|- style="background: #cbcee0;"
 
-
|style="background:#0d3e99; color:white; text-align: right;" width=120px|Description
 
-
|width=700px| clyA fusion (with 5-Gly linker)
 
-
|- style="background: #cbcee0;"
 
-
|style="background:#0d3e99; color:white; text-align: right;"|Sequence (5' > 3')
 
-
|GTTTCTTCCTGCAGCGGCCGCTACTAGTGCCGCCGCCGCCGCCGACTTCAGGTACCTCAAAGAG
 
-
|- style="background: #cbcee0;"
 
-
|style="background:#0d3e99; color:white; text-align: right;"|Designer
 
-
|G. Cambray
 
-
|- style="background: #cbcee0;"
 
-
!colspan="6" style="background:#c5c5c5; text-align: right;" | Not Tested
 
-
|}
 
-
 
-
 
-
 
-
{|
 
-
! colspan="6" style="background: #c5c5c5;" | [O11] ompA_signal_Fw
 
-
|- style="background: #cbcee0;"
 
-
|style="background:#0d3e99; color:white; text-align: right;" width=120px|Description
 
-
|width=700px| ompA signal for export to periplasm - from expression vector RBS as in pIN-III-ompA-2 vector - keeps vector original RBS
 
-
|- style="background: #cbcee0;"
 
-
|style="background:#0d3e99; color:white; text-align: right;"|Sequence (5' > 3')
 
-
|GTTTCTTCGAATTCGCGGCCGCTTCTAGAGAACGAGGGCAAAAAATGAAAAAG
 
-
|- style="background: #cbcee0;"
 
-
|style="background:#0d3e99; color:white; text-align: right;"|Designer
 
-
|G. Cambray
 
-
|- style="background: #cbcee0;"
 
-
!colspan="6" style="background:#c5c5c5; text-align: right;" | Not Tested
 
-
|}
 
-
 
-
 
-
 
-
{|
 
-
! colspan="6" style="background: #c5c5c5;" | [O12] ompA_signal_fusion_Rw
 
-
|- style="background: #cbcee0;"
 
-
|style="background:#0d3e99; color:white; text-align: right;" width=120px|Description
 
-
|width=700px| ompA signal for export to periplasm - from expression vector pIN-III-ompA-2 - original RBS from the vector
 
-
|- style="background: #cbcee0;"
 
-
|style="background:#0d3e99; color:white; text-align: right;"|Sequence (5' > 3')
 
-
|GTTTCTTCCTGCAGCGGCCGCTACTAGTGGCCTGCGCTACGGTAGCGAAAC
 
-
|- style="background: #cbcee0;"
 
-
|style="background:#0d3e99; color:white; text-align: right;"|Designer
 
-
|G. Cambray
 
-
|- style="background: #cbcee0;"
 
-
!colspan="6" style="background:#c5c5c5; text-align: right;" | Not Tested
 
-
|}
 
-
 
-
 
-
{|
 
-
! colspan="6" style="background: #c5c5c5;" | [O13] tolR_II_Fusion_Fw
 
-
|- style="background: #cbcee0;"
 
-
|style="background:#0d3e99; color:white; text-align: right;" width=120px|Description
 
-
|width=700px| For fusion of tolR domain II (standard 23)
 
-
|- style="background: #cbcee0;"
 
-
|style="background:#0d3e99; color:white; text-align: right;"|Sequence (5' > 3')
 
-
|GTTTCTTCGAATTCGCGGCCGCTTCTAGAGAGGTCGATCTGCCAGACGCTAC
 
-
|- style="background: #cbcee0;"
 
-
|style="background:#0d3e99; color:white; text-align: right;"|Designer
 
-
|G. Cambray
 
-
|- style="background: #cbcee0;"
 
-
!colspan="6" style="background:#c5c5c5; text-align: right;" | Not Tested
 
-
|}
 
-
 
-
 
-
{|
 
-
! colspan="6" style="background: #c5c5c5;" | [O14] tatABC_RBS_Fw
 
-
|- style="background: #cbcee0;"
 
-
|style="background:#0d3e99; color:white; text-align: right;" width=120px|Description
 
-
|width=700px| Amplification from tatA - contains RBS
 
-
|- style="background: #cbcee0;"
 
-
|style="background:#0d3e99; color:white; text-align: right;"|Sequence (5' > 3')
 
-
|TTGAATTCGCGGCCGCTTCTAGAAGGAGGTATATAATGATGGGTGGTATCAGTATTTGG
 
-
|- style="background: #cbcee0;"
 
-
|style="background:#0d3e99; color:white; text-align: right;"|Designer
 
-
|G. Cambray
 
-
|- style="background: #cbcee0;"
 
-
!colspan="6" style="background:#c5c5c5; text-align: right;" | Not Tested
 
-
|}
 
-
 
-
 
-
{|
 
-
! colspan="6" style="background: #c5c5c5;" | [O15] TE3_fusion_Fw
 
-
|- style="background: #cbcee0;"
 
-
|style="background:#0d3e99; color:white; text-align: right;" width=120px|Description
 
-
|width=700px| Fusion of N-term domain of colicin E3 (standard 23)
 
-
|- style="background: #cbcee0;"
 
-
|style="background:#0d3e99; color:white; text-align: right;"|Sequence (5' > 3')
 
-
|GTTTCTTCGAATTCGCGGCCGCTTCTAGAAGCGGTGGCGATGGACGCGGCCATAAC
 
-
|- style="background: #cbcee0;"
 
-
|style="background:#0d3e99; color:white; text-align: right;"|Designer
 
-
|G. Cambray
 
-
|- style="background: #cbcee0;"
 
-
!colspan="6" style="background:#c5c5c5; text-align: right;" | Not Tested
 
-
|}
 
-
 
-
 
-
{|
 
-
! colspan="6" style="background: #c5c5c5;" | [O16] TE3_Rw
 
-
|- style="background: #cbcee0;"
 
-
|style="background:#0d3e99; color:white; text-align: right;" width=120px|Description
 
-
|width=700px| Amplification of N-term domain of colicin E3 (standard 23)
 
-
|- style="background: #cbcee0;"
 
-
|style="background:#0d3e99; color:white; text-align: right;"|Sequence (5' > 3')
 
-
|GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTAGCGTTCATAATTTCGCTCAG
 
-
|- style="background: #cbcee0;"
 
-
|style="background:#0d3e99; color:white; text-align: right;"|Designer
 
-
|G. Cambray
 
-
|- style="background: #cbcee0;"
 
-
!colspan="6" style="background:#c5c5c5; text-align: right;" | Not Tested
 
-
|}
 
-
 
-
 
-
 
-
{|
 
-
! colspan="6" style="background: #c5c5c5;" | [O17] Tg3p_Rw
 
-
|- style="background: #cbcee0;"
 
-
|style="background:#0d3e99; color:white; text-align: right;" width=120px|Description
 
-
|width=700px| Amplification of N-term domain of M13's g3p
 
-
|- style="background: #cbcee0;"
 
-
|style="background:#0d3e99; color:white; text-align: right;"|Sequence (5' > 3')
 
-
|GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTAACACTGAGTTTCGTCACCAGTAC
 
-
|- style="background: #cbcee0;"
 
-
|style="background:#0d3e99; color:white; text-align: right;"|Designer
 
-
|G. Cambray
 
-
|- style="background: #cbcee0;"
 
-
!colspan="6" style="background:#c5c5c5; text-align: right;" | Not Tested
 
-
|}
 
-
 
-
 
-
{|
 
-
! colspan="6" style="background: #c5c5c5;" | [O18] Tg3p_fusion_Fw
 
-
|- style="background: #cbcee0;"
 
-
|style="background:#0d3e99; color:white; text-align: right;" width=120px|Description
 
-
|width=700px| Fusion of N-term domain of M13's g3p (standard 23)
 
-
|- style="background: #cbcee0;"
 
-
|style="background:#0d3e99; color:white; text-align: right;"|Sequence (5' > 3')
 
-
|GTTTCTTCGAATTCGCGGCCGCTTCTAGAAAAAAATTATTATTCGCAATTCCTTTAG
 
-
|- style="background: #cbcee0;"
 
-
|style="background:#0d3e99; color:white; text-align: right;"|Designer
 
-
|G. Cambray
 
-
|- style="background: #cbcee0;"
 
-
!colspan="6" style="background:#c5c5c5; text-align: right;" | Not Tested
 
-
|}
 
-
 
-
 
-
{|
 
-
! colspan="6" style="background: #c5c5c5;" | [O19] g3p_F
 
-
|- style="background: #cbcee0;"
 
-
|style="background:#0d3e99; color:white; text-align: right;" width=120px|Description
 
-
|width=700px| g3p
 
-
|- style="background: #cbcee0;"
 
-
|style="background:#0d3e99; color:white; text-align: right;"|Sequence (5' > 3')
 
-
|GTTTCTTCGAATTCGCGGCCGCTTCTAGAGCTCCGCTGAAACTGTTGAAAGTTG
 
-
|- style="background: #cbcee0;"
 
-
|style="background:#0d3e99; color:white; text-align: right;"|Designer
 
-
|G. Cambray
 
-
|- style="background: #cbcee0;"
 
-
!colspan="6" style="background:#c5c5c5; text-align: right;" | Not Tested
 
-
|}
 
-
 
-
 
-
{|
 
-
! colspan="6" style="background: #c5c5c5;" | [O20] g3p_R
 
-
|- style="background: #cbcee0;"
 
-
|style="background:#0d3e99; color:white; text-align: right;" width=120px|Description
 
-
|width=700px| g3p
 
-
|- style="background: #cbcee0;"
 
-
|style="background:#0d3e99; color:white; text-align: right;"|Sequence (5' > 3')
 
-
|GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTAAGACTCCTTATTACGCAG
 
-
|- style="background: #cbcee0;"
 
-
|style="background:#0d3e99; color:white; text-align: right;"|Designer
 
-
|G. Cambray
 
-
|- style="background: #cbcee0;"
 
-
!colspan="6" style="background:#c5c5c5; text-align: right;" | Not Tested
 
-
|}
 
-
 
-
 
-
{|
 
-
! colspan="6" style="background: #c5c5c5;" | [O21] Ompa_linker_F
 
-
|- style="background: #cbcee0;"
 
-
|style="background:#0d3e99; color:white; text-align: right;" width=120px|Description
 
-
|width=700px| For standardisation of OmpA linker (BBaK103006)
 
-
|- style="background: #cbcee0;"
 
-
|style="background:#0d3e99; color:white; text-align: right;"|Sequence (5' > 3')
 
-
|TTGAATTCGCGGCCGCTTCTAGAAGGAGGTATATAATGAAAGCTACTAAACTGGTACTGG
 
-
|- style="background: #cbcee0;"
 
-
|style="background:#0d3e99; color:white; text-align: right;"|Designer
 
-
|G. Cambray
 
-
|- style="background: #cbcee0;"
 
-
!colspan="6" style="background:#c5c5c5; text-align: right;" | Not Tested
 
-
|}
 
-
 
-
 
-
{|
 
-
! colspan="6" style="background: #c5c5c5;" | [O22] Ompa_linker_R
 
-
|- style="background: #cbcee0;"
 
-
|style="background:#0d3e99; color:white; text-align: right;" width=120px|Description
 
-
|width=700px| For standardisation of OmpA linker (BBaK103006)
 
-
|- style="background: #cbcee0;"
 
-
|style="background:#0d3e99; color:white; text-align: right;"|Sequence (5' > 3')
 
-
|GTTTCTTCCTGCAGCGGCCGCTACTAGTAGAGCTCCCTCCTCCAGAACC
 
-
|- style="background: #cbcee0;"
 
-
|style="background:#0d3e99; color:white; text-align: right;"|Designer
 
-
|G. Cambray
 
-
|- style="background: #cbcee0;"
 
-
!colspan="6" style="background:#c5c5c5; text-align: right;" | Not Tested
 
-
|}
 
-
 
-
 
-
{|
 
-
! colspan="6" style="background: #c5c5c5;" | [O23] OmpAsignal-TE3_seq_Fw
 
-
|- style="background: #cbcee0;"
 
-
|style="background:#0d3e99; color:white; text-align: right;" width=120px|Description
 
-
|width=700px| ???
 
-
|- style="background: #cbcee0;"
 
-
|style="background:#0d3e99; color:white; text-align: right;"|Sequence (5' > 3')
 
-
|CACAAATAGCGAAAGATGAC
 
-
|- style="background: #cbcee0;"
 
-
|style="background:#0d3e99; color:white; text-align: right;"|Designer
 
-
|G. Cambray
 
-
|- style="background: #cbcee0;"
 
-
!colspan="6" style="background:#c5c5c5; text-align: right;" | Not Tested
 
-
|}
 
-
 
-
 
-
{|
 
-
! colspan="6" style="background: #c5c5c5;" | [O24] OmpAsignal-TE3_seq_Rw
 
-
|- style="background: #cbcee0;"
 
-
|style="background:#0d3e99; color:white; text-align: right;" width=120px|Description
 
-
|width=700px| ???
 
-
|- style="background: #cbcee0;"
 
-
|style="background:#0d3e99; color:white; text-align: right;"|Sequence (5' > 3')
 
-
|ATCATCTGCGGGTAATGATG
 
-
|- style="background: #cbcee0;"
 
-
|style="background:#0d3e99; color:white; text-align: right;"|Designer
 
-
|G. Cambray
 
-
|- style="background: #cbcee0;"
 
-
!colspan="6" style="background:#c5c5c5; text-align: right;" | Not Tested
 
-
|}
 
-
 
-
 
-
{|
 
-
! colspan="6" style="background: #c5c5c5;" | [O25] VAMP2_Seq_Fw
 
-
|- style="background: #cbcee0;"
 
-
|style="background:#0d3e99; color:white; text-align: right;" width=120px|Description
 
-
|width=700px| ???
 
-
|- style="background: #cbcee0;"
 
-
|style="background:#0d3e99; color:white; text-align: right;"|Sequence (5' > 3')
 
-
|ACTCACTATAGGGAGACCACA
 
-
|- style="background: #cbcee0;"
 
-
|style="background:#0d3e99; color:white; text-align: right;"|Designer
 
-
|G. Cambray
 
-
|- style="background: #cbcee0;"
 
-
!colspan="6" style="background:#c5c5c5; text-align: right;" | Not Tested
 
-
|}
 
-
 
-
 
-
{|
 
-
! colspan="6" style="background: #c5c5c5;" | [O26] VAMP2_Seq_Rv
 
-
|- style="background: #cbcee0;"
 
-
|style="background:#0d3e99; color:white; text-align: right;" width=120px|Description
 
-
|width=700px| ???
 
-
|- style="background: #cbcee0;"
 
-
|style="background:#0d3e99; color:white; text-align: right;"|Sequence (5' > 3')
 
-
|GGGCTTTGTTAGCAGCCGGAT
 
-
|- style="background: #cbcee0;"
 
-
|style="background:#0d3e99; color:white; text-align: right;"|Designer
 
-
|G. Cambray
 
-
|- style="background: #cbcee0;"
 
-
!colspan="6" style="background:#c5c5c5; text-align: right;" | Not Tested
 
-
|}
 
-
 
-
 
-
{|
 
-
! colspan="6" style="background: #c5c5c5;" | [O27] ClyA_seq_Fw
 
-
|- style="background: #cbcee0;"
 
-
|style="background:#0d3e99; color:white; text-align: right;" width=120px|Description
 
-
|width=700px| ???
 
-
|- style="background: #cbcee0;"
 
-
|style="background:#0d3e99; color:white; text-align: right;"|Sequence (5' > 3')
 
-
|GCAGCTATTTCCAGTCACAG
 
-
|- style="background: #cbcee0;"
 
-
|style="background:#0d3e99; color:white; text-align: right;"|Designer
 
-
|G. Cambray
 
-
|- style="background: #cbcee0;"
 
-
!colspan="6" style="background:#c5c5c5; text-align: right;" | Not Tested
 
-
|}
 
-
 
-
 
-
{|
 
-
! colspan="6" style="background: #c5c5c5;" | [O28] ClyA_seq_Rw
 
-
|- style="background: #cbcee0;"
 
-
|style="background:#0d3e99; color:white; text-align: right;" width=120px|Description
 
-
|width=700px| ???
 
-
|- style="background: #cbcee0;"
 
-
|style="background:#0d3e99; color:white; text-align: right;"|Sequence (5' > 3')
 
-
|CGCCCGCAGCAATAGAATAG
 
-
|- style="background: #cbcee0;"
 
-
|style="background:#0d3e99; color:white; text-align: right;"|Designer
 
-
|G. Cambray
 
-
|- style="background: #cbcee0;"
 
-
!colspan="6" style="background:#c5c5c5; text-align: right;" | Not Tested
 
-
|}
 
-
 
-
 
-
{|
 
-
! colspan="6" style="background: #c5c5c5;" | [O29] PFecA_Fw
 
-
|- style="background: #cbcee0;"
 
-
|style="background:#0d3e99; color:white; text-align: right;" width=120px|Description
 
-
|width=700px| ???
 
-
|- style="background: #cbcee0;"
 
-
|style="background:#0d3e99; color:white; text-align: right;"|Sequence (5' > 3')
 
-
|GTTTCTTCGAATTCGCGGCCGCTTCTAGAGTACGCGGTACTGGATAAACATTTC
 
-
|- style="background: #cbcee0;"
 
-
|style="background:#0d3e99; color:white; text-align: right;"|Designer
 
-
|G. Cambray
 
-
|- style="background: #cbcee0;"
 
-
!colspan="6" style="background:#c5c5c5; text-align: right;" | Not Tested
 
-
|}
 
-
 
-
 
-
{|
 
-
! colspan="6" style="background: #c5c5c5;" | [O30] PFecA_Rv
 
-
|- style="background: #cbcee0;"
 
-
|style="background:#0d3e99; color:white; text-align: right;" width=120px|Description
 
-
|width=700px| ???
 
-
|- style="background: #cbcee0;"
 
-
|style="background:#0d3e99; color:white; text-align: right;"|Sequence (5' > 3')
 
-
|GTTTCTTCCTGCAGCGGCCGCTACTAGTATTTTTGTTGTGTTCAGCTATGAGT
 
-
|- style="background: #cbcee0;"
 
-
|style="background:#0d3e99; color:white; text-align: right;"|Designer
 
-
|G. Cambray
 
-
|- style="background: #cbcee0;"
 
-
!colspan="6" style="background:#c5c5c5; text-align: right;" | Not Tested
 
-
|}
 
-
 
-
 
-
<html>
 
-
</div>
 
-
<div id="paris_content_boxtop">
 
-
</div>
 
-
<div id="paris_content">
 
</html>
</html>
-
== PCR products ==
 
-
 
-
{|
 
-
|colspan="11" style="background: #660099;"|PCR products
 
-
|- style="background: #0d3e99; text-align: center; color:white;"
 
-
|width=5%| Number
 
-
|width=10%| Name
 
-
|width=10%| Oligo F
 
-
|width=10%| Oligo R
 
-
|width=5%| Tm
 
-
|width=10%| Matrix
 
-
|width=10%| Length
 
-
|width=25%| Comments
 
-
|width=5%| Quantity
 
-
|width=5%| []ng/µL
 
-
|width=5%| Date
 
-
|- style="background: #ccccff; text-align: center;"
 
-
|width=5%| A1
 
-
|width=10%| g3p_std
 
-
|width=10%| [O19] g3p_F
 
-
|width=10%| [O20] g3p_R
 
-
|width=5%| Tm
 
-
|width=10%| Matrix
 
-
|width=10%| Length
 
-
|width=25%| For standardisation of g3p and adding SacI site
 
-
|width=5%| ?
 
-
|width=5%| ? ng/µL
 
-
|width=5%| ?
 
-
|- style="background: #ccccff; text-align: center;"
 
-
|width=5%| A2
 
-
|width=10%| OmpA_L_std
 
-
|width=10%| [O21] Ompa_linker_F
 
-
|width=10%| [O22] Ompa_linker_R
 
-
|width=5%| Tm
 
-
|width=10%| Matrix
 
-
|width=10%| Length
 
-
|width=25%| For standardisation of OmpA linker (BBaK103006)
 
-
|width=5%| ?
 
-
|width=5%| ? ng/µL
 
-
|width=5%| ?
 
-
|}
 
<br>
<br>
-
<br>
 
-
http://parts.mit.edu/r/parts/images/partbypart/icon_terminator.png
 
-
http://parts.mit.edu/images/partbypart/icon_regulatory.png
 
-
http://parts.mit.edu/r/parts/images/partbypart/icon_rbs.png
 
-
http://parts.mit.edu/r/parts/images/partbypart/icon_reporter.png
 
-
http://parts.mit.edu/registry/images/7/7b/Part_icon_cds.png
 
-
http://parts.mit.edu/registry/images/9/9f/Part_icon_dna.png
 
-
http://parts.mit.edu/registry/images/2/2e/Part_icon_generator.png
 
-
http://parts.mit.edu/registry/images/a/ab/Part_icon_plasmid.png
 
-
 
-
<html>
 
-
</div>
 
-
<div id="paris_content_boxtop">
 
-
</div>
 
-
<div id="paris_content">
 
-
</html>
 
-
 
-
== Plasmids: MiniPrep Products ==
 
-
{|
 
-
|- style="background: #c5c5c5;"
 
-
! colspan="5" style="background: #c5c5c5;" | Plasmids: MiniPrep Products
 
-
|- style="background: #0d3e99; text-align: center; color:white;"
 
-
|width=5%| Number
 
-
|width=25%| Name
 
-
|width=50%| Description
 
-
|width=15%| Products in use
 
-
|width=5%| Date
 
-
|- style="background: #ccccff; text-align: center;"
 
-
|width=5%| P1
 
-
|width=25%| pSB2K3
 
-
|width=50%| General plasmid for cloning step after PCR
 
-
|width=15%| Products in use
 
-
|width=5%| [https://2009.igem.org/Team:Paris/3_August_2009 08/03]
 
-
|- style="background: #ccccff; text-align: center;"
 
-
|width=5%| P2
 
-
|width=25%| Bba-J61002
 
-
|width=50%| Plasmid backbone, it allows us to change promoter RFP as promoter
 
-
|width=15%| Products in use
 
-
|width=5%| [https://2009.igem.org/Team:Paris/3_August_2009 08/03]
 
-
|- style="background: #ccccff; text-align: center;"
 
-
|width=5%| P3
 
-
|width=25%| Bba-J32015
 
-
|width=50%| PelB leader sequence
 
-
|width=15%| Products in use
 
-
|width=5%| [https://2009.igem.org/Team:Paris/3_August_2009 08/03]
 
-
|- style="background: #ccccff; text-align: center;"
 
-
|width=5%| P4
 
-
|width=25%| Bba-B0014
 
-
|width=50%| Double terminator bidirectionnal (B0012-B0011)
 
-
|width=15%| Products in use
 
-
|width=5%| [https://2009.igem.org/Team:Paris/3_August_2009 08/03]
 
-
|- style="background: #ccccff; text-align: center;"
 
-
|width=5%| P5
 
-
|width=25%| Bba-I14032
 
-
|width=50%| pLac
 
-
|width=15%| Products in use
 
-
|width=5%| [https://2009.igem.org/Team:Paris/3_August_2009 08/03]
 
-
|- style="background: #ccccff; text-align: center;"
 
-
|width=5%| P6
 
-
|width=25%| BBa_K103006
 
-
|width=50%| OmpA linker (464bp)
 
-
|width=15%| Products in use
 
-
|width=5%| [https://2009.igem.org/Team:Paris/3_August_2009 08/03]
 
-
|- style="background: #ccccff; text-align: center;"
 
-
|width=5%| P7
 
-
|width=25%| Bba-R0040
 
-
|width=50%| TetR repressible promoter
 
-
|width=15%| Products in use
 
-
|width=5%| [https://2009.igem.org/Team:Paris/3_August_2009 08/03]
 
-
|- style="background: #ccccff; text-align: center;"
 
-
|width=5%| P8
 
-
|width=25%| Bba-I0500
 
-
|width=50%| pBad/araC
 
-
|width=15%| Products in use
 
-
|width=5%| [https://2009.igem.org/Team:Paris/3_August_2009 08/03]
 
-
|- style="background: #ccccff; text-align: center;"
 
-
|width=5%| P9
 
-
|width=25%| &Delta;N-Syn1A
 
-
|width=50%| ???
 
-
|width=15%| Products in use
 
-
|width=5%| [https://2009.igem.org/Team:Paris/3_August_2009 08/03]
 
-
|- style="background: #ccccff; text-align: center;"
 
-
|width=5%| P10
 
-
|width=25%| His6-SNAP25
 
-
|width=50%| ???
 
-
|width=15%| Products in use
 
-
|width=5%| [https://2009.igem.org/Team:Paris/3_August_2009 08/03]
 
-
|- style="background: #ccccff; text-align: center;"
 
-
|width=5%| P11
 
-
|width=25%| VAMP2-HiB6
 
-
|width=50%| ???
 
-
|width=15%| Products in use
 
-
|width=5%| [https://2009.igem.org/Team:Paris/3_August_2009 08/03]
 
-
|- style="background: #ccccff; text-align: center;"
 
-
|width=5%| P12
 
-
|width=25%| g3p
 
-
|width=50%| g3p in pHEN2 plasmid
 
-
|width=15%| Products in use
 
-
|width=5%| [https://2009.igem.org/Team:Paris/4_August_2009 08/04]
 
-
|}
 
-
<html>
 
-
</div>
 
-
<div id="paris_content_boxtop">
 
-
</div>
 
-
<div id="paris_content">
 
-
</html>
 
-
 
-
== Digestion Products ==
 
-
<div style="background-color:#FF0000; padding:1px; margin:1px; font-size:120%; text-align:center; font-weight:bold; border:1px solid black">Red = Vector</div>
 
-
<div style="background-color:#FFFF00; padding:1px; margin:1px; font-size:120%; text-align:center; font-weight:bold; border:1px solid black">Yellow = Insert</div>
 
-
 
-
{|
 
-
|- style="background: #c5c5c5;"
 
-
! colspan="10" style="background: #c5c5c5;" | Digestion Products
 
-
|- style="background: #0d3e99; text-align: center; color:white;"
 
-
|width=5%| Number
 
-
|width=20%| Product Name
 
-
|width=20%| Matrix Name
 
-
|width=5%| Enz. 1
 
-
|width=5%| Enz. 2
 
-
|width=5%| Size
 
-
|width=20%| Description
 
-
|width=10%| Product Left
 
-
|width=5%| []ng/µL
 
-
|width=5%| Date
 
-
|- style="background: #ccccff; text-align: center;"
 
-
|style="background:#FFFF00"| D1
 
-
| P3
 
-
| BBa-J32015
 
-
| XbaI
 
-
| PstI
 
-
| Size
 
-
| ???
 
-
| ???
 
-
| ???
 
-
| [https://2009.igem.org/Team:Paris/4_August_2009 08/04]
 
-
|- style="background: #ccccff; text-align: center;"
 
-
|style="background:#FFFF00"| D2
 
-
| P4
 
-
| BBa-B0014
 
-
| XbaI
 
-
| PstI
 
-
| Size
 
-
| ???
 
-
| ???
 
-
| ???
 
-
| [https://2009.igem.org/Team:Paris/4_August_2009 08/04]
 
-
|- style="background: #ccccff; text-align: center;"
 
-
|style="background:#FFFF00"| D3
 
-
| P5
 
-
| BBa-I14032
 
-
| XbaI
 
-
| PstI
 
-
| Size
 
-
| ???
 
-
| ???
 
-
| ???
 
-
| [https://2009.igem.org/Team:Paris/4_August_2009 08/04]
 
-
|- style="background: #ccccff; text-align: center;"
 
-
|style="background:#FFFF00"| D4
 
-
| P7
 
-
| Bba-R0040
 
-
| XbaI
 
-
| PstI
 
-
| Size
 
-
| ???
 
-
| ???
 
-
| ???
 
-
| [https://2009.igem.org/Team:Paris/4_August_2009 08/04]
 
-
|- style="background: #ccccff; text-align: center;"
 
-
|style="background:#FFFF00"| D5
 
-
| P8
 
-
| BBa-10500
 
-
| XbaI
 
-
| PstI
 
-
| Size
 
-
| ???
 
-
| ???
 
-
| ???
 
-
| [https://2009.igem.org/Team:Paris/4_August_2009 08/04]
 
-
|- style="background: #ccccff; text-align: center;"
 
-
|style="background:#FFFF00"| D6
 
-
| ???
 
-
| ???
 
-
| XbaI
 
-
| PstI
 
-
| Size
 
-
| ???
 
-
| ???
 
-
| ???
 
-
| [https://2009.igem.org/Team:Paris/4_August_2009 08/04]
 
-
|- style="background: #ccccff; text-align: center;"
 
-
|style="background:#FFFF00"| D7
 
-
| ???
 
-
| ???
 
-
| XbaI
 
-
| PstI
 
-
| Size
 
-
| ???
 
-
| ???
 
-
| ???
 
-
| [https://2009.igem.org/Team:Paris/4_August_2009 08/04]
 
-
|}
 
-
<html>
 
-
</div>
 
-
<div id="paris_content_boxtop">
 
-
</div>
 
-
<div id="paris_content">
 
-
</html>
 
-
 
-
== Unexpected digestion results ==
 
-
{|
 
-
|- style="background: #c5c5c5;"
 
-
! colspan="7" style="background: #c5c5c5;" | Digestion Products
 
-
|- style="background: #0d3e99; text-align: center; color:white;"
 
-
|width=5%| Number
 
-
|width=30%| Matrix Name
 
-
|width=5%| Enz. 1
 
-
|width=5%| Enz. 2
 
-
|width=10%| Expected Size
 
-
|width=40%| Description
 
-
|width=5%| Date
 
-
|}
 
-
 
-
 
-
<html>
 
-
</div>
 
-
<div id="paris_content_boxtop">
 
-
</div>
 
-
<div id="paris_content">
 
-
</html>
 
-
 
-
== Strains (Glycerol stock) ==
 
{|
{|
|- style="background: #c5c5c5;"
|- style="background: #c5c5c5;"
-
! colspan="5" style="background: #c5c5c5;" | Strains glycerol stock
+
! colspan="8" style="background:#c5c5c5; text-align: center" | Color code for sticker
-
|- style="background: #0d3e99; text-align: center; color:white;"
+
|- style="background: #0d3e99; text-align: center; color:black;"
-
|width=5%| Number
+
|width=10% style="background: #FF0000;"| Vector
-
|Width=2%|R
+
|width=10% style="background: #FFFF00;"| Insert
-
|width=25%| Name
+
|width=10% style="background: #660099;"| PCR product
-
|width=55%| Description
+
|width=10% style="background: #FF6600;"| Plasmid
-
|width=5%| Date
+
|width=10% style="background: #FFFFFF;"| Strains
-
|- style="background: #cbcee0; text-align: center;"
+
|width=10% style="background: #006400;"| Kanamycin
-
|<strike>[https://2009.igem.org/Team:Paris/Strains#Strain_1 S1]</strike>
+
|width=10% style="background: #3333FF;"| Ampicilin
-
|style="background:#cccccc;"|
+
|width=10% style="background: #FF0066;"| Chloranphenicol
-
|<strike>DH5&alpha;</strike>
+
-
|<strike>DH5&alpha; E. Coli strain for chimically competent</strike>
+
-
|[https://2009.igem.org/Team:Paris/20_July_2009 07/20]
+
-
|- style="background: #cbcee0; text-align: center;"
+
-
|[https://2009.igem.org/Team:Paris/Strains#Strain_2 S2]
+
-
|style="background:#006400;"|K
+
-
|K12 MG 1655 &Delta;recA::Kan
+
-
|Standard E.Coli strain, Kan resistance
+
-
|[https://2009.igem.org/Team:Paris/20_July_2009 07/20]
+
-
|- style="background: #cbcee0; text-align: center;"
+
-
|[https://2009.igem.org/Team:Paris/Strains#Strain_3 S3]
+
-
|style="background:#006400;"|K
+
-
|W3110 &Delta;nlp1
+
-
|From Daniel Rodriguez, Kan resistance
+
-
|[https://2009.igem.org/Team:Paris/23_July_2009 07/23]
+
-
|- style="background: #cbcee0; text-align: center;"
+
-
|[https://2009.igem.org/Team:Paris/Strains#TOP10 TOP10]
+
-
|style="background:#cccccc;"|  
+
-
|TOP10
+
-
|Competent bacteria
+
-
|[https://2009.igem.org/Team:Paris/27_July_2009 07/27]
+
-
|- style="background: #cbcee0; text-align: center;"
+
-
|[https://2009.igem.org/Team:Paris/Strains#Strain_4 S4]
+
-
|style="background:#cccccc;"|  
+
-
|TB28
+
-
|No kanamycin resistance
+
-
|[https://2009.igem.org/Team:Paris/29_July_2009 07/29]
+
-
|- style="background: #cbcee0; text-align: center;"
+
-
|[https://2009.igem.org/Team:Paris/Strains#Strain_5 S5]
+
-
|style="background:#006400;"|K
+
-
|MG4
+
-
|Strain needs to be grown on media with [NaCl]>=0.5%, preferably 1%NaCl
+
-
|[https://2009.igem.org/Team:Paris/29_July_2009 07/29]
+
-
|- style="background: #cbcee0; text-align: center;"
+
-
|[https://2009.igem.org/Team:Paris/Strains#Strain_6 S6]
+
-
|style="background:#cccccc;"|
+
-
|DH5&alpha;
+
-
|DH5&alpha; E. Coli strain for chimically competent (Pasteur)
+
-
|[https://2009.igem.org/Team:Paris/30_July_2009 07/30]
+
-
|- style="background: #cbcee0; text-align: center;"
+
-
|[https://2009.igem.org/Team:Paris/Strains#Strain_7 S7]
+
-
|style="background:#3333FF;"|A
+
-
|DH5&alpha;(g3p)
+
-
|DH5&alpha; E. Coli strain with g3p in plasmid, Amp resistance
+
-
|[https://2009.igem.org/Team:Paris/30_July_2009 07/30]
+
-
|- style="background: #cbcee0; text-align: center;"
+
-
|[https://2009.igem.org/Team:Paris/Strains#Strain_8 S8]
+
-
|style="background:#006400;"|K
+
-
|DH5&alpha;(pSB2K3)
+
-
|DH5&alpha; E. Coli strain with pSB2K3 plasmid, Kan resistance
+
-
|[https://2009.igem.org/Team:Paris/3_August_2009 08/03]
+
-
|- style="background: #cbcee0; text-align: center;"
+
-
|[https://2009.igem.org/Team:Paris/Strains#Strain_9 S9]
+
-
|style="background:#3333FF;"|A
+
-
|DH5&alpha;(BBa_J61002)
+
-
|DH5&alpha; E. Coli strain with BBa_J61002 in plasmid, Amp resistance
+
-
|[https://2009.igem.org/Team:Paris/3_August_2009 08/03]
+
-
|- style="background: #cbcee0; text-align: center;"
+
-
|[https://2009.igem.org/Team:Paris/Strains#Strain_10 S10]
+
-
|style="background:#006400;"|K
+
-
|DH5&alpha;(BBa_J32015)
+
-
|DH5&alpha; E. Coli strain with BBa_J32015 in plasmid, Kan resistance
+
-
|[https://2009.igem.org/Team:Paris/3_August_2009 08/03]
+
-
|- style="background: #cbcee0; text-align: center;"
+
-
|[https://2009.igem.org/Team:Paris/Strains#Strain_11 S11]
+
-
|style="background:#006400;"|K
+
-
|DH5&alpha;(BBa_B0014)
+
-
|DH5&alpha; E. Coli strain with BBa_B0014 in plasmid, Kan resistance
+
-
|[https://2009.igem.org/Team:Paris/3_August_2009 08/03]
+
-
|- style="background: #cbcee0; text-align: center;"
+
-
|[https://2009.igem.org/Team:Paris/Strains#Strain_12 S12]
+
-
|style="background:#006400;"|K
+
-
|DH5&alpha;(BBa_I14032)
+
-
|DH5&alpha; E. Coli strain with BBa_I14032 in plasmid, Kan resistance
+
-
|[https://2009.igem.org/Team:Paris/3_August_2009 08/03]
+
-
|- style="background: #cbcee0; text-align: center;"
+
-
|[https://2009.igem.org/Team:Paris/Strains#Strain_13 S13]
+
-
|style="background:#006400;"|K
+
-
|DH5&alpha;(BBa_K103006)
+
-
|DH5&alpha; E. Coli strain with BBa_K103006 in plasmid, Kan resistance
+
-
|[https://2009.igem.org/Team:Paris/3_August_2009 08/03]
+
-
|- style="background: #cbcee0; text-align: center;"
+
-
|[https://2009.igem.org/Team:Paris/Strains#Strain_14 S14]
+
-
|style="background:#3333FF;"|A
+
-
|DH5&alpha;(BBa_R0040)
+
-
|DH5&alpha; E. Coli strain with BBa_R0040 in plasmid, Amp resistance
+
-
|[https://2009.igem.org/Team:Paris/3_August_2009 08/03]
+
-
|- style="background: #cbcee0; text-align: center;"
+
-
|[https://2009.igem.org/Team:Paris/Strains#Strain_15 S15]
+
-
|style="background:#006400;"|K
+
-
|DH5&alpha;(BBa_10500)
+
-
|DH5&alpha; E. Coli strain with BBa_10500 in plasmid, Kan resistance
+
-
|[https://2009.igem.org/Team:Paris/3_August_2009 08/03]
+
-
|- style="background: #cbcee0; text-align: center;"
+
-
|[https://2009.igem.org/Team:Paris/Strains#Strain_16 S16]
+
-
|style="background:#006400;"|K
+
-
|DH5&alpha;(&Delta;N-Syn1A)
+
-
|DH5&alpha; E. Coli strain with &Delta;N-Syn1A in plasmid, Kan resistance
+
-
|[https://2009.igem.org/Team:Paris/3_August_2009 08/03]
+
-
|- style="background: #cbcee0; text-align: center;"
+
-
|[https://2009.igem.org/Team:Paris/Strains#Strain_17 S17]
+
-
|style="background:#3333FF;"|A
+
-
|DH5&alpha;(His6-SNAP25)
+
-
|DH5&alpha; E. Coli strain with His6-SNAP25 in plasmid, Amp resistance
+
-
|[https://2009.igem.org/Team:Paris/3_August_2009 08/03]
+
-
|- style="background: #cbcee0; text-align: center;"
+
-
|[https://2009.igem.org/Team:Paris/Strains#Strain_18 S18]
+
-
|style="background:#3333FF;"|A
+
-
|DH5&alpha;(Vamp2-His6)
+
-
|DH5&alpha; E. Coli strain with Vamp2-His6 in plasmid, Amp resistance
+
-
|[https://2009.igem.org/Team:Paris/3_August_2009 08/03]
+
-
|- style="background: #cbcee0; text-align: center;"
+
-
|[https://2009.igem.org/Team:Paris/Strains#Strain_19 S19]
+
-
|style="background:#3333FF;"|A
+
-
|DH5&alpha;(puc19)
+
-
|DH5&alpha; E. Coli strain with puc19 plasmid, Amp resistance
+
-
|[https://2009.igem.org/Team:Paris/30_July_2009 07/30]
+
|}
|}

Latest revision as of 15:29, 19 October 2009

iGEM > Paris > Freezer > Main


Freezer


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