Team:Paris/4 August 2009

From 2009.igem.org

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==Brain work==
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===Brain work===
A lot of meeting today: First on for the modeling explication and application of stochastic model ?
A lot of meeting today: First on for the modeling explication and application of stochastic model ?
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==Lab work==
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===Lab work===
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<div style="background-color:#7B96B3; border:1px solid #333333; font-weight:bold; padding:3px 5px; margin-bottom:1px">
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<div class="guillaume">
Overnight culture
Overnight culture
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</div><div style="background-color:#E0E9EF; border:1px solid #333333; padding:3px 5px; margin-bottom:10px">
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</div>
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<div class="experience">
*Keio Collection
*Keio Collection
-
**[S20] JW4251: LB Kan1000x 200rpm 37°C
+
**[https://2009.igem.org/Team:Paris/Freezer_Strains_(Glycerol_stock)#S20 S20] JW4251: LB Kan1000x 200rpm 37°C
-
**[S21] JN0729: LB Kan1000x 200rpm 37°C
+
**[https://2009.igem.org/Team:Paris/Freezer_Strains_(Glycerol_stock)#S21 S21] JN0729: LB Kan1000x 200rpm 37°C
-
**[S22] JN0728: LB Kan1000x 200rpm 37°C
+
**[https://2009.igem.org/Team:Paris/Freezer_Strains_(Glycerol_stock)#S22 S22] JN0728: LB Kan1000x 200rpm 37°C
-
**[S23] JN0727: LB Kan1000x 200rpm 37°C
+
**[https://2009.igem.org/Team:Paris/Freezer_Strains_(Glycerol_stock)#S23 S23] JN0727: LB Kan1000x 200rpm 37°C
-
**[S24] JN0940: LB Kan1000x 200rpm 37°C
+
**[https://2009.igem.org/Team:Paris/Freezer_Strains_(Glycerol_stock)#S24 S24] JN0940: LB Kan1000x 200rpm 37°C
-
**[S24] JN0940: LB Kan1000x 37°C
+
**[https://2009.igem.org/Team:Paris/Freezer_Strains_(Glycerol_stock)#S24 S24] JN0940: LB Kan1000x 37°C
-
**[S25] JW5100: LB Kan1000x 200rpm 37°C
+
**[https://2009.igem.org/Team:Paris/Freezer_Strains_(Glycerol_stock)#S25 S25] JW5100: LB Kan1000x 200rpm 37°C
-
**[S25] JW5100: LB Kan1000x 37°C
+
**[https://2009.igem.org/Team:Paris/Freezer_Strains_(Glycerol_stock)#S25 S25] JW5100: LB Kan1000x 37°C
-
**[S26] JN5181: LB Kan1000x 200rpm 37°C
+
**[https://2009.igem.org/Team:Paris/Freezer_Strains_(Glycerol_stock)#S26 S26] JN5181: LB Kan1000x 200rpm 37°C
-
*[S27] FF64: LB Tet1000x 200rpm 37°C
+
*[https://2009.igem.org/Team:Paris/Freezer_Strains_(Glycerol_stock)#S27 S27] FF64: LB Tet1000x 200rpm 37°C
-
*[S28] NEC280: LB 200rpm 37°C
+
*[https://2009.igem.org/Team:Paris/Freezer_Strains_(Glycerol_stock)#S28 S28] NEC280: LB 200rpm 37°C
-
*[S5] MG4: LB Kan NaCl20% 200rpm 37°C
+
*[https://2009.igem.org/Team:Paris/Freezer_Strains_(Glycerol_stock)#S5 S5] MG4: LB Kan NaCl20% 200rpm 37°C
-
*[S6] DH5&alpha;: LB 200rpm 37°C
+
*[https://2009.igem.org/Team:Paris/Freezer_Strains_(Glycerol_stock)#S6 S6] DH5&alpha;: LB 200rpm 37°C
<br>
<br>
-
*[S24] JN0940: LBagar plate with Kan1000x 37°C
+
*[https://2009.igem.org/Team:Paris/Freezer_Strains_(Glycerol_stock)#S24 S24] JN0940: LBagar plate with Kan1000x 37°C
-
*[S25] JW5100: LBagar plate with Kan1000x 37°C
+
*[https://2009.igem.org/Team:Paris/Freezer_Strains_(Glycerol_stock)#S25 S25] JW5100: LBagar plate with Kan1000x 37°C
</div>
</div>
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<div style="background-color:#7B96B3; border:1px solid #333333; font-weight:bold; padding:3px 5px; margin-bottom:1px">
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<div class="guillaume">
Biobrick resuspention
Biobrick resuspention
-
</div><div style="background-color:#E0E9EF; border:1px solid #333333; padding:3px 5px; margin-bottom:10px">
+
</div>
 +
<div class="experience">
*1/1K named 1K1 (pSB1A3). Resistant to Ampicilin.
*1/1K named 1K1 (pSB1A3). Resistant to Ampicilin.
</div>
</div>
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<div style="background-color:#7B96B3; border:1px solid #333333; font-weight:bold; padding:3px 5px; margin-bottom:1px">
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<div class="guillaume">
Transformation of precited Biobrick  
Transformation of precited Biobrick  
-
</div><div style="background-color:#E0E9EF; border:1px solid #333333; padding:3px 5px; margin-bottom:10px">
 
-
*1K1 (pSB1A3) in competent DH5&alpha; by RbCl [[https://2009.igem.org/Team:Paris/Protocols_transformation Protocol]]
 
</div>
</div>
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<div style="background-color:#7B96B3; border:1px solid #333333; font-weight:bold; padding:3px 5px; margin-bottom:1px">
+
<div class="experience">
 +
*1K1 (pSB1A3) in competent DH5&alpha; by [[team:Paris/Protocols_Culture#RbCl|RbCl]]
 +
</div>
 +
<div class="vicard">
Purification
Purification
-
</div><div style="background-color:#E0E9EF; border:1px solid #333333; padding:3px 5px; margin-bottom:10px">
+
</div>
 +
<div class="experience">
*Purification of P3,P4,P5,P7 with  XbaI PstI (using 1,5% agarose because pb is inferior to 100pb during 25 min)And P8 with (1%Agarose during 25 min because pb is superior to 1kpb).  
*Purification of P3,P4,P5,P7 with  XbaI PstI (using 1,5% agarose because pb is inferior to 100pb during 25 min)And P8 with (1%Agarose during 25 min because pb is superior to 1kpb).  
-
D1=[P3[https://2009.igem.org/Team:Paris/Freezer_Plasmids]]Digested, D2=P4, D3=P5, D4=P7, D5=P8, (D4 seems to be bad because we don't see is specific band during a gel after is purification)
+
*D1=[https://2009.igem.org/Team:Paris/Freezer_Plasmids#P3 P3] Digested=66pb, D2=[https://2009.igem.org/Team:Paris/Freezer_Plasmids#P4 P4] Digested=95pb, D3=[https://2009.igem.org/Team:Paris/Freezer_Plasmids#P5 P5] Digested=37pb, D4=[https://2009.igem.org/Team:Paris/Freezer_Plasmids#P7 P7] Digested=54pb, D5=[https://2009.igem.org/Team:Paris/Freezer_Plasmids#P8 P8] Digested=1210pb, (D4 seems to be bad because we don't see his specific band during a gel after is purification)
-
**P1 didn't work (we don't have the specific band after the digestion so we can't putificate it), P2 purification, for tomorrow
+
*P1 didn't work (we don't have the specific band after the digestion so we can't putificate it), P2 purification, for tomorrow
*Gel for check the purification[Ladder 100bp|P3|P4|P5|P7|nothing|ladder 1kb|P8]
*Gel for check the purification[Ladder 100bp|P3|P4|P5|P7|nothing|ladder 1kb|P8]
<center>[[Image:4aout1er_o.JPG]]</center>
<center>[[Image:4aout1er_o.JPG]]</center>
 +
*D1=Works, D2=Works, D3=Doesn't work(his band not correspond at 37pb), D4=Doesn't work(no band), D5=Work not well (2 bands)
</div>
</div>
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<html>
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==To do list==
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===To do list===
{|
{|
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|Lab/ g3p
|Lab/ g3p
|}
|}
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 +
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{{Paris2009_Calendar_Link|3_August_2009|5_August_2009}}

Latest revision as of 13:33, 10 August 2009

Contents

NoteBook

June
MTWTFSS
[http://2009.igem.org/Team:Paris/1_June_2009 1] [http://2009.igem.org/wiki/index.php?title=Team:Paris/2_June_2009&action=edit 2] [http://2009.igem.org/wiki/index.php?title=Team:Paris/3_June_2009&action=edit 3] [http://2009.igem.org/wiki/index.php?title=Team:Paris/4_June_2009&action=edit 4] [http://2009.igem.org/wiki/index.php?title=Team:Paris/5_June_2009&action=edit 5] [http://2009.igem.org/wiki/index.php?title=Team:Paris/6_June_2009&action=edit 6] [http://2009.igem.org/wiki/index.php?title=Team:Paris/7_June_2009&action=edit 7]
[http://2009.igem.org/wiki/index.php?title=Team:Paris/8_June_2009&action=edit 8] [http://2009.igem.org/wiki/index.php?title=Team:Paris/9_June_2009&action=edit 9] [http://2009.igem.org/wiki/index.php?title=Team:Paris/10_June_2009&action=edit 10] [http://2009.igem.org/wiki/index.php?title=Team:Paris/11_June_2009&action=edit 11] [http://2009.igem.org/wiki/index.php?title=Team:Paris/12_June_2009&action=edit 12] [http://2009.igem.org/Team:Paris/13_June_2009 13] [http://2009.igem.org/wiki/index.php?title=Team:Paris/14_June_2009&action=edit 14]
[http://2009.igem.org/wiki/index.php?title=Team:Paris/15_June_2009&action=edit 15] [http://2009.igem.org/wiki/index.php?title=Team:Paris/16_June_2009&action=edit 16] [http://2009.igem.org/wiki/index.php?title=Team:Paris/17_June_2009&action=edit 17] [http://2009.igem.org/wiki/index.php?title=Team:Paris/18_June_2009&action=edit 18] [http://2009.igem.org/wiki/index.php?title=Team:Paris/19_June_2009&action=edit 19] [http://2009.igem.org/wiki/index.php?title=Team:Paris/20_June_2009&action=edit 20] [http://2009.igem.org/wiki/index.php?title=Team:Paris/21_June_2009&action=edit 21]
[http://2009.igem.org/wiki/index.php?title=Team:Paris/22_June_2009&action=edit 22] [http://2009.igem.org/Team:Paris/23_June_2009 23] [http://2009.igem.org/wiki/index.php?title=Team:Paris/24_June_2009&action=edit 24] [http://2009.igem.org/wiki/index.php?title=Team:Paris/25_June_2009&action=edit 25] [http://2009.igem.org/wiki/index.php?title=Team:Paris/26_June_2009&action=edit 26] [http://2009.igem.org/wiki/index.php?title=Team:Paris/27_June_2009&action=edit 27] [http://2009.igem.org/wiki/index.php?title=Team:Paris/28_June_2009&action=edit 28]
[http://2009.igem.org/wiki/index.php?title=Team:Paris/29_June_2009&action=edit 29] [http://2009.igem.org/Team:Paris/30_June_2009 30]
July
MTWTFSS
    [http://2009.igem.org/wiki/index.php?title=Team:Paris/1_July_2009&action=edit 1] [http://2009.igem.org/wiki/index.php?title=Team:Paris/2_July_2009&action=edit 2] [http://2009.igem.org/Team:Paris/3_July_2009 3] [http://2009.igem.org/wiki/index.php?title=Team:Paris/4_July_2009&action=edit 4] [http://2009.igem.org/wiki/index.php?title=Team:Paris/5_July_2009&action=edit 5]
[http://2009.igem.org/wiki/index.php?title=Team:Paris/6_July_2009&action=edit 6] [http://2009.igem.org/wiki/index.php?title=Team:Paris/7_July_2009&action=edit 7] [http://2009.igem.org/wiki/index.php?title=Team:Paris/8_July_2009&action=edit 8] [http://2009.igem.org/wiki/index.php?title=Team:Paris/9_July_2009&action=edit 9] [http://2009.igem.org/wiki/index.php?title=Team:Paris/10_July_2009&action=edit 10] [http://2009.igem.org/wiki/index.php?title=Team:Paris/11_July_2009&action=edit 11] [http://2009.igem.org/wiki/index.php?title=Team:Paris/12_July_2009&action=edit 12]
[http://2009.igem.org/Team:Paris/13_July_2009 13] [http://2009.igem.org/Team:Paris/14_July_2009 14] [http://2009.igem.org/Team:Paris/15_July_2009 15] [http://2009.igem.org/Team:Paris/16_July_2009 16] [http://2009.igem.org/Team:Paris/17_July_2009 17] [http://2009.igem.org/Team:Paris/18_July_2009 18] [http://2009.igem.org/Team:Paris/19_July_2009 19]
[http://2009.igem.org/Team:Paris/20_July_2009 20] [http://2009.igem.org/Team:Paris/21_July_2009 21] [http://2009.igem.org/Team:Paris/22_July_2009 22] [http://2009.igem.org/Team:Paris/23_July_2009 23] [http://2009.igem.org/Team:Paris/24_July_2009 24] [http://2009.igem.org/Team:Paris/25_July_2009 25] [http://2009.igem.org/Team:Paris/26_July_2009 26]
[http://2009.igem.org/Team:Paris/27_July_2009 27] [http://2009.igem.org/Team:Paris/28_July_2009 28] [http://2009.igem.org/Team:Paris/29_July_2009 29] [http://2009.igem.org/Team:Paris/30_July_2009 30] [http://2009.igem.org/Team:Paris/31_July_2009 31]
August
MTWTFSS
          [http://2009.igem.org/Team:Paris/1_August_2009 1] [http://2009.igem.org/Team:Paris/2_August_2009 2]
[http://2009.igem.org/Team:Paris/3_August_2009 3] [http://2009.igem.org/Team:Paris/4_August_2009 4] [http://2009.igem.org/Team:Paris/5_August_2009 5] [http://2009.igem.org/Team:Paris/6_August_2009 6] [http://2009.igem.org/Team:Paris/7_August_2009 7] [http://2009.igem.org/Team:Paris/8_August_2009 8] [http://2009.igem.org/Team:Paris/9_August_2009 9]
[http://2009.igem.org/Team:Paris/10_August_2009 10] [http://2009.igem.org/Team:Paris/11_August_2009 11] [http://2009.igem.org/Team:Paris/12_August_2009 12] [http://2009.igem.org/Team:Paris/13_August_2009 13] [http://2009.igem.org/Team:Paris/14_August_2009 14] [http://2009.igem.org/Team:Paris/15_August_2009 15] [http://2009.igem.org/Team:Paris/16_August_2009 16]
[http://2009.igem.org/Team:Paris/17_August_2009 17] [http://2009.igem.org/Team:Paris/18_August_2009 18] [http://2009.igem.org/Team:Paris/19_August_2009 19] [http://2009.igem.org/Team:Paris/20_August_2009 20] [http://2009.igem.org/Team:Paris/21_August_2009 21] [http://2009.igem.org/Team:Paris/22_August_2009 22] [http://2009.igem.org/Team:Paris/23_August_2009 23]
[http://2009.igem.org/Team:Paris/24_August_2009 24] [http://2009.igem.org/Team:Paris/25_August_2009 25] [http://2009.igem.org/Team:Paris/26_August_2009 26] [http://2009.igem.org/Team:Paris/27_August_2009 27] [http://2009.igem.org/Team:Paris/28_August_2009 28] [http://2009.igem.org/Team:Paris/29_August_2009 29] [http://2009.igem.org/Team:Paris/30_August_2009 30]
[http://2009.igem.org/Team:Paris/31_August_2009 31]
September
MTWTFSS
  [http://2009.igem.org/Team:Paris/1_September_2009 1] [http://2009.igem.org/Team:Paris/2_September_2009 2] [http://2009.igem.org/Team:Paris/3_September_2009 3] [http://2009.igem.org/Team:Paris/4_September_2009 4] [http://2009.igem.org/Team:Paris/5_September_2009 5] [http://2009.igem.org/Team:Paris/6_September_2009 6]
[http://2009.igem.org/Team:Paris/7_September_2009 7] [http://2009.igem.org/Team:Paris/8_September_2009 8] [http://2009.igem.org/Team:Paris/9_September_2009 9] [http://2009.igem.org/Team:Paris/10_September_2009 10] [http://2009.igem.org/Team:Paris/11_September_2009 11] [http://2009.igem.org/Team:Paris/12_September_2009 12] [http://2009.igem.org/Team:Paris/13_September_2009 13]
[http://2009.igem.org/Team:Paris/14_September_2009 14] [http://2009.igem.org/Team:Paris/15_September_2009 15] [http://2009.igem.org/Team:Paris/16_September_2009 16] [http://2009.igem.org/Team:Paris/17_September_2009 17] [http://2009.igem.org/Team:Paris/18_September_2009 18] [http://2009.igem.org/Team:Paris/19_September_2009 19] [http://2009.igem.org/Team:Paris/20_September_2009 20]
[http://2009.igem.org/Team:Paris/21_September_2009 21] [http://2009.igem.org/Team:Paris/22_September_2009 22] [http://2009.igem.org/Team:Paris/23_September_2009 23] [http://2009.igem.org/Team:Paris/24_September_2009 24] [http://2009.igem.org/Team:Paris/25_September_2009 25] [http://2009.igem.org/Team:Paris/26_September_2009 26] [http://2009.igem.org/Team:Paris/27_September_2009 27]
[http://2009.igem.org/Team:Paris/28_September_2009 28] [http://2009.igem.org/Team:Paris/29_September_2009 29] [http://2009.igem.org/Team:Paris/30_September_2009 30]
October
MTWTFSS
      [http://2009.igem.org/Team:Paris/1_October_2009 1] [http://2009.igem.org/Team:Paris/2_October_2009 2] [http://2009.igem.org/Team:Paris/3_October_2009 3] [http://2009.igem.org/Team:Paris/4_October_2009 4]
[http://2009.igem.org/Team:Paris/5_October_2009 5] [http://2009.igem.org/Team:Paris/6_October_2009 6] [http://2009.igem.org/Team:Paris/7_October_2009 7] [http://2009.igem.org/Team:Paris/8_October_2009 8] [http://2009.igem.org/Team:Paris/9_October_2009 9] [http://2009.igem.org/Team:Paris/10_October_2009 10] [http://2009.igem.org/Team:Paris/11_October_2009 11]
[http://2009.igem.org/Team:Paris/12_October_2009 12] [http://2009.igem.org/Team:Paris/13_October_2009 13] [http://2009.igem.org/Team:Paris/14_October_2009 14] [http://2009.igem.org/Team:Paris/15_October_2009 15] [http://2009.igem.org/Team:Paris/16_October_2009 16] [http://2009.igem.org/Team:Paris/17_October_2009 17] [http://2009.igem.org/Team:Paris/18_October_2009 18]
[http://2009.igem.org/Team:Paris/19_October_2009 19] [http://2009.igem.org/Team:Paris/20_October_2009 20] [http://2009.igem.org/Team:Paris/21_October_2009 21] [http://2009.igem.org/Team:Paris/22_October_2009 22] [http://2009.igem.org/Team:Paris/23_October_2009 23] [http://2009.igem.org/Team:Paris/24_October_2009 24] [http://2009.igem.org/Team:Paris/25_October_2009 25]
[http://2009.igem.org/Team:Paris/26_October_2009 26] [http://2009.igem.org/Team:Paris/27_October_2009 27] [http://2009.igem.org/Team:Paris/28_October_2009 28] [http://2009.igem.org/Team:Paris/29_October_2009 29] [http://2009.igem.org/Team:Paris/30_October_2009 30] [http://2009.igem.org/Team:Paris/31_October_2009 31]

← Yesterday - Tomorrow →

August 4th

Brain work

A lot of meeting today: First on for the modeling explication and application of stochastic model ? The second one on every parts and each member's work explanation. We still arguing about the travel agency for the Jamboree...Waaaaaaaaa

Lab work

Overnight culture

  • Keio Collection
    • S20 JW4251: LB Kan1000x 200rpm 37°C
    • S21 JN0729: LB Kan1000x 200rpm 37°C
    • S22 JN0728: LB Kan1000x 200rpm 37°C
    • S23 JN0727: LB Kan1000x 200rpm 37°C
    • S24 JN0940: LB Kan1000x 200rpm 37°C
    • S24 JN0940: LB Kan1000x 37°C
    • S25 JW5100: LB Kan1000x 200rpm 37°C
    • S25 JW5100: LB Kan1000x 37°C
    • S26 JN5181: LB Kan1000x 200rpm 37°C
  • S27 FF64: LB Tet1000x 200rpm 37°C
  • S28 NEC280: LB 200rpm 37°C
  • S5 MG4: LB Kan NaCl20% 200rpm 37°C
  • S6 DH5α: LB 200rpm 37°C


  • S24 JN0940: LBagar plate with Kan1000x 37°C
  • S25 JW5100: LBagar plate with Kan1000x 37°C

Biobrick resuspention

  • 1/1K named 1K1 (pSB1A3). Resistant to Ampicilin.

Transformation of precited Biobrick

  • 1K1 (pSB1A3) in competent DH5α by RbCl

Purification

  • Purification of P3,P4,P5,P7 with XbaI PstI (using 1,5% agarose because pb is inferior to 100pb during 25 min)And P8 with (1%Agarose during 25 min because pb is superior to 1kpb).
  • D1=P3 Digested=66pb, D2=P4 Digested=95pb, D3=P5 Digested=37pb, D4=P7 Digested=54pb, D5=P8 Digested=1210pb, (D4 seems to be bad because we don't see his specific band during a gel after is purification)
  • P1 didn't work (we don't have the specific band after the digestion so we can't putificate it), P2 purification, for tomorrow
  • Gel for check the purification[Ladder 100bp|P3|P4|P5|P7|nothing|ladder 1kb|P8]
4aout1er o.JPG
  • D1=Works, D2=Works, D3=Doesn't work(his band not correspond at 37pb), D4=Doesn't work(no band), D5=Work not well (2 bands)

To do list

Matricule TODO
Luc SNARE Construction
Romain Design oligo Fec A
Charlotte design of oligos: FecR, FecA, SNARE?, sequencing oligo
Stoff Dev schedule algorithm / BDD
Chris modeling : we don't want fu*king oscillations !
Lisa Oligo Tol/Pal / Oligo Sequencing / Tol/Pal Protocol verification / Keio / Theme A writting
Caroline coloration of cells / protocols for the lab: control of the emission of vesicules....
Souf oligos desing / Labs
Vicard Gel / SNARE
Pierre modeling : we don't want fu*king oscillations !
Sylvain minipreps. Check if the use of starch is possible ; problem : find a system to help the starch enter into the periplasm, use of LacY -> noise
Guillaume Lab/ g3p


← Yesterday - Tomorrow →

Retrieved from "http://2009.igem.org/Team:Paris/4_August_2009"