Team:Paris/Protocols

From 2009.igem.org

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<span/ id="bottom">[https://2009.igem.org/ iGEM ] > [[Team:Paris#top | Paris]] > [[Team:Paris/Protocols#top | Protocols]] > [[Team:Paris/Protocols#bottom | Main]]
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== Protocols ==
 
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Here you will find the collection of protocole we use, or just collect. And because we are well-known chauvinist it is just a tribute to previous Paris iGEM team .
 
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We try our best not to make it redondant.
 
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<center> [[team:Paris/Protocols#Protocols|Main]] - [[Team:Paris/ProtocolsA#Protocols | Microscopy Protocol]] - [[Team:Paris/ProtocolsB#Protocols | Adapted Protocols]] - [[Team:Paris/Protocols_Culture#Protocols | Culture]] - [[Team:Paris/ProtocolsMB#Protocols | Molecular Biology]]</center>
 
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==Main==
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===Summary===
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====1. Microscopy====
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* Protocols suggested
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====2. Adapted Protocols====
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* DNA extraction
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* Mini prep
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* Gel/PCR Purification
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====3. Culture protocols====
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* Over Night
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* Transformation
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* [[Team:Paris/Protocols_Competent_Bacteria#Protocols | Competent bacterias]]
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* Glycerol Stock
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====4. Molecular biology====
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* PCR
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* Gels
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* Digestion
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* Ligation
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*[[Team:Paris/Protocols_Competent_Bacteria | Protocol to make competent bacteria]]
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*[[Team:Paris/Protocols_PCRqload | Protocole PCR quick load Taq mix]]
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*[http://probes.invitrogen.com/media/pis/mp00282.pdf Protocol to membrane dying, DID method]
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*[[Team:Paris/Miniprep | Adaptation of PureYield&trade; Plasmid Miniprep System]]
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*[[Team:Paris/Protocols_transformation | Protocol for bacterial transformation]]
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<div id="middle-side"><center>
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<a class="menu_sub_active"href="https://2009.igem.org/Team:Paris/Protocols#bottom"> Main </a>|
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<a class="menu_sub" href="https://2009.igem.org/Team:Paris/ProtocolsA#bottom"> Microscope Protocols</a>|
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<a class="menu_sub"href="https://2009.igem.org/Team:Paris/ProtocolsB#bottom"> Adapted Protocols</a>|
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<a class="menu_sub"href="https://2009.igem.org/Team:Paris/Protocols_Culture#bottom"> Culture Protocols</a>|
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<a class="menu_sub"href="https://2009.igem.org/Team:Paris/ProtocolsMB#bottom"> Molecular biology</a>
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</center>
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====A.2. iGEM paris 2008 protocols link====
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[https://2008.igem.org/Team:Paris/Notebook/Protocols Click here]
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===1. Microscopy===
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* [[Team:Paris/ProtocolsA#Protocols suggested | Protocols suggested]]
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*[http://probes.invitrogen.com/media/pis/mp00282.pdf Protocol to membrane dying, DID method (pdf)]
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<u>content :</u>
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===2. Adapted Protocols===
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* DNA extraction
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* [[Team:Paris/ProtocolsB#Adaptation of PureYield&trade; Plasmid Miniprep System (Promega) |Mini prep ]]
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*  Gel/PCR Purification
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*Electrophoresis
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===3. Culture protocols===
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*Concentration of the Miniprep or the Midiprep
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* Over Night
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*Amplification of promoters.
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*[[Team:Paris/Protocols_Culture#Bacterial transformation | Bacterial transformation]]
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*PCR Screening
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* [[Team:Paris/Protocols_Culture#Competent bacteria | Competent bacteria]]
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*Protocol to make competent bacteria
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**[[team:Paris/Protocols_Culture#CaCl2a|CaCl<sub>2</sub> (iGEM Paris2007)]]
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**[[team:Paris/Protocols_Culture#CaCl2b|CaCl<sub>2</sub> (2nd Protocol)]]
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**[[team:Paris/Protocols_Culture#RbCl|RbCl]]
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* [[team:Paris/Protocols_Culture#Glycerol stock|Glycerol stock]]
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*[[team:Paris/Protocols_Culture#Ferric citrate growth medium|Ferric citrate growth medium]]
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===4. Molecular biology===
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====A.3. iGEM paris 2007 protocols link====  
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*[[Team:Paris/ProtocolsMB#PCR with Quick load Taq2x Master Mix|PCR]]
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*[[Team:Paris/ProtocolsMB#PCR colonies|PCR colony]]
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[http://parts.mit.edu/igem07/index.php/Paris/PROTOCOLS Click here]
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* Gels
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* Digestion
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* Ligation
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<u>content :</u>
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*Growing_bacteria_in_liquid_medium
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*Preparing growth media
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**exemple: Making 10 petri dish (LB+erythromycin+citrate+DAP), Solid M9 Minimum Medium, preparation of agarosis gel
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*Chemical transformation
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*Glycerol Stock
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*Recombineering/Lambda red-mediated gene replacement
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*Miniprep
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*Fluorescent single cells visualisation
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*Digestion reactions
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Latest revision as of 16:35, 20 October 2009

iGEM > Paris > Protocols > Main


Contents

Main

1. Microscopy

  • Protocols suggested
  • [http://probes.invitrogen.com/media/pis/mp00282.pdf Protocol to membrane dying, DID method (pdf)]

2. Adapted Protocols

3. Culture protocols

4. Molecular biology

Retrieved from "http://2009.igem.org/Team:Paris/Protocols"