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Team:Paris/4 August 2009
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| - | *1K1 (pSB1A3) in competent DH5α by | + | *1K1 (pSB1A3) in competent DH5α by [[team:Paris/Protocols_Culture#RbCl|RbCl]] |
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*Purification of P3,P4,P5,P7 with XbaI PstI (using 1,5% agarose because pb is inferior to 100pb during 25 min)And P8 with (1%Agarose during 25 min because pb is superior to 1kpb). | *Purification of P3,P4,P5,P7 with XbaI PstI (using 1,5% agarose because pb is inferior to 100pb during 25 min)And P8 with (1%Agarose during 25 min because pb is superior to 1kpb). | ||
| - | *D1= | + | *D1=[https://2009.igem.org/Team:Paris/Freezer_Plasmids#P3 P3] Digested=66pb, D2=[https://2009.igem.org/Team:Paris/Freezer_Plasmids#P4 P4] Digested=95pb, D3=[https://2009.igem.org/Team:Paris/Freezer_Plasmids#P5 P5] Digested=37pb, D4=[https://2009.igem.org/Team:Paris/Freezer_Plasmids#P7 P7] Digested=54pb, D5=[https://2009.igem.org/Team:Paris/Freezer_Plasmids#P8 P8] Digested=1210pb, (D4 seems to be bad because we don't see his specific band during a gel after is purification) |
*P1 didn't work (we don't have the specific band after the digestion so we can't putificate it), P2 purification, for tomorrow | *P1 didn't work (we don't have the specific band after the digestion so we can't putificate it), P2 purification, for tomorrow | ||
*Gel for check the purification[Ladder 100bp|P3|P4|P5|P7|nothing|ladder 1kb|P8] | *Gel for check the purification[Ladder 100bp|P3|P4|P5|P7|nothing|ladder 1kb|P8] | ||
Latest revision as of 13:33, 10 August 2009
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NoteBook
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Brain work
A lot of meeting today: First on for the modeling explication and application of stochastic model ? The second one on every parts and each member's work explanation. We still arguing about the travel agency for the Jamboree...Waaaaaaaaa
Lab work
Overnight culture
- Keio Collection
- S20 JW4251: LB Kan1000x 200rpm 37°C
- S21 JN0729: LB Kan1000x 200rpm 37°C
- S22 JN0728: LB Kan1000x 200rpm 37°C
- S23 JN0727: LB Kan1000x 200rpm 37°C
- S24 JN0940: LB Kan1000x 200rpm 37°C
- S24 JN0940: LB Kan1000x 37°C
- S25 JW5100: LB Kan1000x 200rpm 37°C
- S25 JW5100: LB Kan1000x 37°C
- S26 JN5181: LB Kan1000x 200rpm 37°C
- S27 FF64: LB Tet1000x 200rpm 37°C
- S28 NEC280: LB 200rpm 37°C
- S5 MG4: LB Kan NaCl20% 200rpm 37°C
- S6 DH5α: LB 200rpm 37°C
Biobrick resuspention
- 1/1K named 1K1 (pSB1A3). Resistant to Ampicilin.
Transformation of precited Biobrick
- 1K1 (pSB1A3) in competent DH5α by RbCl
Purification
- Purification of P3,P4,P5,P7 with XbaI PstI (using 1,5% agarose because pb is inferior to 100pb during 25 min)And P8 with (1%Agarose during 25 min because pb is superior to 1kpb).
- D1=P3 Digested=66pb, D2=P4 Digested=95pb, D3=P5 Digested=37pb, D4=P7 Digested=54pb, D5=P8 Digested=1210pb, (D4 seems to be bad because we don't see his specific band during a gel after is purification)
- P1 didn't work (we don't have the specific band after the digestion so we can't putificate it), P2 purification, for tomorrow
- Gel for check the purification[Ladder 100bp|P3|P4|P5|P7|nothing|ladder 1kb|P8]
- D1=Works, D2=Works, D3=Doesn't work(his band not correspond at 37pb), D4=Doesn't work(no band), D5=Work not well (2 bands)
To do list
| Matricule | TODO |
| Luc | SNARE Construction |
| Romain | Design oligo Fec A |
| Charlotte | design of oligos: FecR, FecA, SNARE?, sequencing oligo |
| Stoff | Dev schedule algorithm / BDD |
| Chris | modeling : we don't want fu*king oscillations ! |
| Lisa | Oligo Tol/Pal / Oligo Sequencing / Tol/Pal Protocol verification / Keio / Theme A writting |
| Caroline | coloration of cells / protocols for the lab: control of the emission of vesicules.... |
| Souf | oligos desing / Labs |
| Vicard | Gel / SNARE |
| Pierre | modeling : we don't want fu*king oscillations ! |
| Sylvain | minipreps. Check if the use of starch is possible ; problem : find a system to help the starch enter into the periplasm, use of LacY -> noise |
| Guillaume | Lab/ g3p |
