Team:Paris/constructions
From 2009.igem.org
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+ | <span/ id="bottom">[https://2009.igem.org/ iGEM ] > [[Team:Paris#top | Paris]] > [[Team:Paris/Freezer#top | Freezer]] > [[Team:Paris/constructions#bottom | Constructions in progress]] | ||
{{Template:Paris2009}} | {{Template:Paris2009}} | ||
{{Template:Paris2009_menu}} | {{Template:Paris2009_menu}} | ||
- | |||
- | <center> | + | == Constructions == |
- | + | <html> | |
- | + | <style type="text/css"> | |
- | + | #left-side { | |
- | + | position: absolute; | |
- | + | height: 23px; | |
- | + | width: 30px; | |
+ | top: 0px; | ||
+ | left: 10px; | ||
+ | margin-top:10px; | ||
+ | padding-top: 7px; | ||
+ | background: url(https://static.igem.org/mediawiki/2009/1/1b/Left_menu_pari.png); | ||
+ | z-index:4; | ||
+ | } | ||
+ | |||
+ | #middle-side { | ||
+ | height: 25px; | ||
+ | width: 660px; | ||
+ | position: absolute; | ||
+ | top: 0px; | ||
+ | left: 30px; | ||
+ | margin-top:10px; | ||
+ | padding-top: 5px; | ||
+ | background: #dadada; | ||
+ | z-index:5; | ||
+ | } | ||
+ | |||
+ | #right-side { | ||
+ | position: absolute; | ||
+ | height: 23px; | ||
+ | width: 30px; | ||
+ | margin-top:10px; | ||
+ | padding-top: 7px; | ||
+ | top: 0px; | ||
+ | left: 670px; | ||
+ | background: url(https://static.igem.org/mediawiki/2009/4/40/Right_menu_paris.png); | ||
+ | z-index:4; | ||
+ | } | ||
+ | |||
+ | a.menu_sub { | ||
+ | padding-left: 7px; | ||
+ | padding-right: 7px; | ||
+ | } | ||
+ | |||
+ | a.menu_sub_active { | ||
+ | padding-left: 7px; | ||
+ | padding-right: 7px; | ||
+ | color:#b0310e; | ||
+ | font-weight:bold; | ||
+ | } | ||
+ | </style> | ||
+ | <div id="left-side"></div> | ||
+ | <div id="middle-side"><center> | ||
+ | <a class="menu_sub"href="https://2009.igem.org/Team:Paris/Freezer#bottom"> Main </a>| | ||
+ | <a class="menu_sub" href="https://2009.igem.org/Team:Paris/Freezer_PCR_products#bottom"> PCR Products</a>| | ||
+ | <a class="menu_sub"href="https://2009.igem.org/Team:Paris/Freezer_Plasmids#bottom"> Plasmids</a>| | ||
+ | <a class="menu_sub"href="https://2009.igem.org/Team:Paris/Freezer_Digestion_Products#bottom"> Digestion Products</a>| | ||
+ | <a class="menu_sub"href="https://2009.igem.org/Team:Paris/Freezer_Strains_(Glycerol_stock)#bottom"> Strains (Glycerol stock)</a>| | ||
+ | <a class="menu_sub_active"href="https://2009.igem.org/Team:Paris/constructions#bottom"> Constructions in progress</a> | ||
</center> | </center> | ||
- | < | + | </div> |
+ | |||
+ | <div id="right-side"></div> | ||
+ | |||
+ | </html> | ||
+ | |||
+ | |||
+ | |||
{| | {| | ||
|- style="background: #c5c5c5;" | |- style="background: #c5c5c5;" | ||
Line 26: | Line 85: | ||
|width=10% style="background: #FF0066;"| Chloranphenicol | |width=10% style="background: #FF0066;"| Chloranphenicol | ||
|} | |} | ||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | + | ||
+ | *'''Plasmid A''' | ||
+ | |||
+ | |||
{| | {| | ||
|- style="background: #0d3e99; text-align: center; color:white;" | |- style="background: #0d3e99; text-align: center; color:white;" | ||
Line 43: | Line 99: | ||
|width=4%| Registry | |width=4%| Registry | ||
- | |- style="background: # | + | |- style="background: #FF9933; text-align: center" |
- | | | + | |CC |
- | |( | + | |(Pbad :: rbs - lacI - LVA) in [http://partsregistry.org/Part:pSB1A3 pSB1A3]???Pbad is on PSB2K3 |
- | + | |[http://partsregistry.org/Part:BBa_I0500 Bba_I0500] :: [http://partsregistry.org/Part:BBa_J24679 BBa_J24679] in [http://partsregistry.org/Part:pSB2K3 pSB2K3] | |
- | | | + | |pSB1A3 transformed, digested, PBad transformed and not digested well- LacI transformed and digested (10/08/09) |
- | | | + | |?? |
- | | | + | |BBa_???? |
- | |- style="background: # | + | |- style="background: #FF9933; text-align: center" |
|CC | |CC | ||
- | | | + | |(Pbad :: rbs - lacI - LVA) :: ter in [http://partsregistry.org/Part:pSB1A3 pSB1A3] |
- | | | + | |BBa_??? :: [http://partsregistry.org/Part:BBa_B0014 BBa_B0014] in [http://partsregistry.org/Part:pSB2K3 pSB2K3] |
- | | | + | |ter transformed, digested and purified (10/08/09) |
|?? | |?? | ||
|BBa_???? | |BBa_???? | ||
- | |- style="background: # | + | |- style="background: #FF9933; text-align: center" |
|CC | |CC | ||
- | |( | + | |((Pbad :: rbs - lacI - LVA) :: RFP) in [http://partsregistry.org/Part:pSB1A3 pSB1A3] |
- | |BBa_ | + | |BBa_??? :: [http://partsregistry.org/wiki/index.php?title=Part:BBa_K093005 BBa_K093005] in [http://partsregistry.org/Part:pSB2K3 pSB2K3] |
- | | | + | |pSB2K3 transformed and digested, RFP : primers designed but not yet ordered (10/08/09) |
|?? | |?? | ||
|BBa_???? | |BBa_???? | ||
- | |- style="background: # | + | |
+ | |- style="background: #FF9933; text-align: center" | ||
|CC | |CC | ||
- | | | + | |(((Pbad :: rbs - lacI - LVA) :: RFP) :: ter) in [http://partsregistry.org/Part:pSB1A3 pSB1A3] |
- | |[http://partsregistry.org/Part: | + | |BBa_??? :: [http://partsregistry.org/Part:BBa_B0014 BBa_B0014] in [http://partsregistry.org/Part:pSB2K3 pSB2K3] |
| | | | ||
|?? | |?? | ||
|BBa_???? | |BBa_???? | ||
- | |- style="background: # | + | |
+ | |- style="background: #FFCC00; text-align: center" | ||
|CC | |CC | ||
- | | | + | |(OmpA Linker :: g3p) in [http://partsregistry.org/Part:pSB2K3 pSB2K3] |
- | |[http://partsregistry.org/Part: | + | |[http://partsregistry.org/Part:BBa_K103006 BBa_K103006] :: BBa_??? in [http://partsregistry.org/Part:pSB2K3 pSB2K3] |
+ | |OmpA linker standardisé - OmpA amplified, not purified - Same for g3p (10/08/09) | ||
+ | |?? | ||
+ | |BBa_???? | ||
+ | |||
+ | |- style="background: #FFCC00; text-align: center" | ||
| | | | ||
+ | |(g3p :: clyA) in [http://partsregistry.org/Part:pSB2K3 pSB2K3] | ||
+ | |BBa_??? :: BBa_??? in [http://partsregistry.org/Part:pSB2K3 pSB2K3] | ||
+ | |autre possibilité (souf)- 1 primer missing for C term fusion with clyA (10/08/09) | ||
|?? | |?? | ||
|BBa_???? | |BBa_???? | ||
- | |- style="background: # | + | |- style="background: #FFCC00; text-align: center" |
- | |CC | + | |CC! |
- | | | + | |((OmpA Linker :: g3p) :: ter) in [http://partsregistry.org/Part:pSB2K3 pSB2K3] |
- | |BBa_??? :: [http://partsregistry.org/ | + | | BBa_??? :: [http://partsregistry.org/Part:BBa_B0014 BBa_B0014] in [http://partsregistry.org/Part:pSB2K3 pSB2K3] |
| | | | ||
|?? | |?? | ||
|BBa_???? | |BBa_???? | ||
- | |- style="background: # | + | |- style="background: #FFCC00; text-align: center" |
- | |CC | + | |CC! |
- | | | + | |((???autotransporter??? - jun/foss) :: ter) in [http://partsregistry.org/Part:pSB2K3 pSB2K3] |
- | |[http://partsregistry.org/Part: | + | | BBa_??? :: [http://partsregistry.org/Part:BBa_B0014 BBa_B0014] in [http://partsregistry.org/Part:pSB2K3 pSB2K3] |
+ | |jun/foss has to be synthetized & autotransporter to be found!!!! (10/08/09) | ||
+ | |?? | ||
+ | |BBa_???? | ||
+ | |||
+ | |- style="background: #FF0000; text-align: center" | ||
+ | |CC! | ||
+ | |((rbs - fecA) :: ((rbs - jun/foss) :: ter)) in [http://partsregistry.org/Part:pSB2K3 pSB2K3] | ||
+ | |BBa_??? :: BBa_??? in [http://partsregistry.org/Part:pSB2K3 pSB2K3] | ||
+ | |FecA : oligo ordered but not received yet (10/08/09) | ||
+ | |?? | ||
+ | |BBa_???? | ||
+ | |||
+ | |- style="background: #FF0000; text-align: center" | ||
+ | |CC! | ||
+ | |((rbs - fecA) :: ((Ompa Linker :: g3p) :: ter)) in [http://partsregistry.org/Part:pSB2K3 pSB2K3] | ||
+ | |BBa_??? :: BBa_??? in [http://partsregistry.org/Part:pSB2K3 pSB2K3] | ||
| | | | ||
|?? | |?? | ||
Line 101: | Line 183: | ||
|- style="background: #FF0000; text-align: center" | |- style="background: #FF0000; text-align: center" | ||
|CC | |CC | ||
- | | | + | |((rbs - ClyA :: RFP) in [http://partsregistry.org/Part:pSB2K3 pSB2K3] |
- | | | + | |BBa_??? :: BBa_???? in [http://partsregistry.org/Part:pSB2K3 pSB2K3] |
- | | | + | |modif RFP (souf)- some troubles with amplification of clyA fusion C term (10/08/09) |
|?? | |?? | ||
|BBa_???? | |BBa_???? | ||
Line 109: | Line 191: | ||
|- style="background: #FF0000; text-align: center" | |- style="background: #FF0000; text-align: center" | ||
|CC | |CC | ||
- | |( | + | |((rbs-RFP :: clyA) in [http://partsregistry.org/Part:pSB2K3 pSB2K3] |
- | |BBa_??? :: [http://partsregistry.org/ | + | |BBa_??? :: BBa_???? in [http://partsregistry.org/Part:pSB2K3 pSB2K3] |
+ | |modif RFP (souf) - fusion N term de cly A (autre possibilité)- we still miss 1 primer for clyA N term fusion (10/08/09) | ||
+ | |?? | ||
+ | |BBa_???? | ||
+ | |||
+ | |- style="background: #FF0000; text-align: center" | ||
+ | |CC! | ||
+ | |((rbs - ClyA :: RFP)) :: (((rbs - fecA) :: (rbs - jun/foss)) :: ter)) in [http://partsregistry.org/Part:pSB2K3 pSB2K3] | ||
+ | |BBa_??? :: BBa_???? in [http://partsregistry.org/Part:pSB2K3 pSB2K3] | ||
+ | |modif RFP (souf) | ||
+ | |?? | ||
+ | |BBa_???? | ||
+ | |||
+ | |- style="background: #FF0000; text-align: center" | ||
+ | |CC! | ||
+ | |((rbs - ClyA :: RFP) :: (((rbs - fecA) :: (OmpA linker :: g3p)) :: ter)) in [http://partsregistry.org/Part:pSB2K3 pSB2K3] | ||
+ | |BBa_??? :: BBa_???? in [http://partsregistry.org/Part:pSB2K3 pSB2K3] | ||
+ | |modif RFP (souf) | ||
+ | |?? | ||
+ | |BBa_???? | ||
+ | |||
+ | |- style="background: #CC0000; text-align: center" | ||
+ | |CC! | ||
+ | |(Pbad :: ((rbs - ClyA :: RFP) :: (((rbs - fecA) :: (OmpA linker :: g3p)) :: ter))) in [http://partsregistry.org/Part:pSB2K3 pSB2K3] | ||
+ | |[http://partsregistry.org/Part:BBa_I0500 Bba_I0500] :: BBa_???? in [http://partsregistry.org/Part:pSB2K3 pSB2K3] | ||
| | | | ||
|?? | |?? | ||
|BBa_???? | |BBa_???? | ||
+ | |- style="background: #CC0000; text-align: center" | ||
+ | |CC! | ||
+ | |(Pbad :: ((rbs - ClyA :: RFP) :: (((rbs - fecA) :: (rbs - jun/foss)) :: ter))) in [http://partsregistry.org/Part:pSB2K3 pSB2K3] | ||
+ | |BBa_??? :: BBa_???? in [http://partsregistry.org/Part:pSB2K3 pSB2K3] | ||
+ | | | ||
+ | |?? | ||
+ | |BBa_???? | ||
- | |- style="background: # | + | |- style="background: #990000; text-align: center; color:white;" |
- | |CC | + | |CC! |
- | |(rbs - fecA) :: (rbs - jun/foss) in [http://partsregistry.org/Part:pSB2K3 pSB2K3] | + | |((Pbad :: ((rbs - ClyA :: RFP) :: (((rbs - fecA) :: (OmpA linker :: g3p)) :: ter))) :: ((Pbad :: rbs - lacI - LVA) :: ter)) in [http://partsregistry.org/Part:pSB2K3 pSB2K3] |
- | |BBa_??? :: BBa_??? in [http://partsregistry.org/Part:pSB2K3 pSB2K3] | + | |BBa_??? :: BBa_???? in [http://partsregistry.org/Part:pSB2K3 pSB2K3] |
+ | |2*Pbad et 2*ter?? (souf) | ||
+ | |?? | ||
+ | |BBa_???? | ||
+ | |||
+ | |- style="background: #990000; text-align: center; color:white;" | ||
+ | |CC! | ||
+ | |((Pbad :: ((rbs - ClyA :: RFP) :: (((rbs - fecA) :: (rbs - jun/foss)) :: ter))) :: ((Pbad :: rbs - lacI - LVA) :: ter)) in [http://partsregistry.org/Part:pSB2K3 pSB2K3] | ||
+ | |BBa_??? :: BBa_???? in [http://partsregistry.org/Part:pSB2K3 pSB2K3] | ||
| | | | ||
|?? | |?? | ||
|BBa_???? | |BBa_???? | ||
- | |- style="background: # | + | |
+ | |} | ||
+ | |||
+ | |||
+ | *'''Plasmid B''' | ||
+ | |||
+ | |||
+ | {| | ||
+ | |- style="background: #0d3e99; text-align: center; color:white;" | ||
+ | |width=3%| Designer | ||
+ | |width=40%| Parts description | ||
+ | |width=25%| Corresponding BioBricks | ||
+ | |width=25%| Status (last update) | ||
+ | |width=3%| Plasmid # | ||
+ | |width=4%| Registry | ||
+ | |||
+ | |- style="background: #ccccff; text-align: center" | ||
+ | |GC <span/ id="ptet"> | ||
+ | |((Plac :: rbs-tetR) :: rbs-tolRII) in pSB2A3 | ||
+ | |BBa_blabla :: BBa_tralala in truc | ||
+ | |Planning / oligo desgned or ordered / PCR in progress / cloned / sequencing... (10/08/09) | ||
+ | |12 | ||
+ | |BBa_pouet pouet | ||
+ | |||
+ | |- style="background: #ccff00; text-align: center" | ||
|CC | |CC | ||
- | | | + | |(Plac :: rbs-tetR) in [http://partsregistry.org/Part:pSB1A3 pSB1A3] |
- | |[http://partsregistry.org/Part: | + | |[http://partsregistry.org/Part:BBa_I14032 Bba_I14032] :: [http://partsregistry.org/Part:BBa_K136050 BBa_K136050] in [http://partsregistry.org/Part:pSB1A3 pSB1A3] |
- | | | + | |both have been digested and purified, ligation in progress (10/08/09) |
|?? | |?? | ||
|BBa_???? | |BBa_???? | ||
- | |- style="background: # | + | |- style="background: #ccff00; text-align: center" |
|CC | |CC | ||
- | |(rbs - | + | |((Plac :: rbs-tetR) :: ter) in [http://partsregistry.org/Part:pSB1A3 pSB1A3] |
- | |BBa_??? :: | + | |BBa_???? :: [http://partsregistry.org/Part:BBa_B0014 BBa_B0014] in [http://partsregistry.org/Part:pSB1A3 pSB1A3] |
| | | | ||
|?? | |?? | ||
- | | | + | |[http://partsregistry.org/wiki/index.php?title=Part:BBa_K257015 BBa_K257015] |
- | |- style="background: # | + | |- style="background: #ccff00; text-align: center" |
|CC | |CC | ||
- | |( | + | |(ter :: ((Plac :: rbs-tetR) :: ter)) in [http://partsregistry.org/Part:pSB1A3 pSB1A3] |
- | | | + | |[http://partsregistry.org/Part:BBa_B0014 BBa_B0014] :: [http://partsregistry.org/wiki/index.php?title=Part:BBa_K257015 BBa_K257015] in [http://partsregistry.org/Part:pSB1A3 pSB1A3] |
| | | | ||
|?? | |?? | ||
|BBa_???? | |BBa_???? | ||
- | |- style="background: # | + | |- style="background: #00FF00; text-align: center" |
+ | |CL | ||
+ | |(rbs - OmpAsignal :: TolR II) | ||
+ | |[http://partsregistry.org/wiki/index.php?title=Part:BBa_K257006 BBa_K257006] ::[http://partsregistry.org/wiki/index.php?title=Part:BBa_K257005 BBa_K257005] in [http://partsregistry.org/Part:pSB1A3 pSB1A3] | ||
+ | |OmpA signal and TolRII amplified, but neither digested nor purified (10/08/09) | ||
+ | |?? | ||
+ | |BBa_???? | ||
+ | |||
+ | |- style="background: #00FF00; text-align: center" | ||
+ | |CC <span/ id="tolr"> | ||
+ | |(Ptet :: (rbs - OmpAsignal :: TolRII)) in [http://partsregistry.org/Part:pSB1A3 pSB1A3] | ||
+ | |[http://partsregistry.org/Part:BBa_R0040 Bba_R0040] :: [http://partsregistry.org/wiki/index.php?title=Part:BBa_K257006 BBa_K257006] ::[http://partsregistry.org/wiki/index.php?title=Part:BBa_K257005 BBa_K257005] in [http://partsregistry.org/Part:pSB1A3 pSB1A3] | ||
+ | |Ptet digested and purified (10/08/09) | ||
+ | |?? | ||
+ | |BBa_???? | ||
+ | |||
+ | |- style="background: #00FF00; text-align: center" | ||
|CC | |CC | ||
- | | | + | |(rbs :: EYFP) in [http://partsregistry.org/Part:pSB1A3 pSB1A3] |
- | |BBa_??? :: [http://partsregistry.org/Part: | + | |BBa_??? :: [http://partsregistry.org/wiki/index.php?title=Part:BBa_E0030 BBa_E0030] in [http://partsregistry.org/Part:pSB1A3 pSB1A3] |
- | | | + | |y a t-il vraiment 2 biobricks ou bien met-on le RBS direct dans des oligos pour RFP? (souf) biobrick to find or primers to design (10/08/09) |
|?? | |?? | ||
|BBa_???? | |BBa_???? | ||
- | |- style="background: # | + | |- style="background: #00FF00; text-align: center" |
|CC | |CC | ||
- | |( | + | |(Ptet :: (rbs :: EYFP)) in [http://partsregistry.org/Part:pSB1A3 pSB1A3] |
- | | | + | |[http://partsregistry.org/Part:BBa_R0040 Bba_R0040] :: BBa_??? in [http://partsregistry.org/Part:pSB1A3 pSB1A3] |
| | | | ||
|?? | |?? | ||
|BBa_???? | |BBa_???? | ||
- | |- style="background: # | + | |- style="background: #006600; text-align: center; color:white;" |
|CC | |CC | ||
- | |( | + | |((Ptet :: (rbs :: EYFP)) :: (ter :: ((Plac :: rbs-tetR) :: ter))) in [http://partsregistry.org/Part:pSB1A3 pSB1A3] |
- | |BBa_??? :: BBa_ | + | |BBa_??? :: BBa_??? in [http://partsregistry.org/Part:pSB1A3 pSB1A3] |
| | | | ||
+ | |?? | ||
+ | |BBa_???? | ||
+ | |||
+ | |- style="background: #006600; text-align: center; color:white;" | ||
+ | |CC <span/ id="tolr2"> | ||
+ | |((Ptet :: (rbs - OmpAsignal :: TolRIIP)) :: (ter :: ((Plac :: rbs-tetR) :: ter))) in [http://partsregistry.org/Part:pSB1A3 pSB1A3] | ||
+ | |BBa_??? :: BBa_??? in [http://partsregistry.org/Part:pSB1A3 pSB1A3] | ||
+ | | | ||
+ | |?? | ||
+ | |BBa_???? | ||
+ | |||
+ | |} | ||
+ | |||
+ | |||
+ | |||
+ | *'''Plasmid C''' | ||
+ | |||
+ | |||
+ | {| | ||
+ | |- style="background: #0d3e99; text-align: center; color:white;" | ||
+ | |width=3%| Designer | ||
+ | |width=40%| Parts description | ||
+ | |width=25%| Corresponding BioBricks | ||
+ | |width=25%| Status (last update) | ||
+ | |width=3%| Plasmid # | ||
+ | |width=4%| Registry | ||
+ | |||
+ | |- style="background: #9966FF; text-align: center; color:black;" | ||
+ | |CC | ||
+ | |(rbs-FecI :: rbs-FecR) in [http://partsregistry.org/Part:pSB2K3 pSB2K3] | ||
+ | |BBa_??? :: BBa_??? | ||
+ | |primers ordered but not received yet (10/08/09) | ||
+ | |?? | ||
+ | |BBa_???? | ||
+ | |||
+ | |||
+ | |- style="background: #9966FF; text-align: center; color:black;" | ||
+ | |CC | ||
+ | |PfecA in [http://partsregistry.org/Part:BBa_J61002 Bba_J61002] | ||
+ | |BBa_??? | ||
+ | |vector digested and purified, Pfec A amplified but not digested (purified?) (10/08/09) | ||
|?? | |?? | ||
|BBa_???? | |BBa_???? | ||
|} | |} |
Latest revision as of 21:51, 21 October 2009
iGEM > Paris > Freezer > Constructions in progress
Constructions
Color code for sticker | |||||||
---|---|---|---|---|---|---|---|
Vector | Insert | PCR product | Plasmid | Strains | Kanamycin | Ampicilin | Chloranphenicol |
- Plasmid A
Designer | Parts description | Corresponding BioBricks | Status (last update) | Plasmid # | Registry |
CC | (Pbad :: rbs - lacI - LVA) in [http://partsregistry.org/Part:pSB1A3 pSB1A3]???Pbad is on PSB2K3 | [http://partsregistry.org/Part:BBa_I0500 Bba_I0500] :: [http://partsregistry.org/Part:BBa_J24679 BBa_J24679] in [http://partsregistry.org/Part:pSB2K3 pSB2K3] | pSB1A3 transformed, digested, PBad transformed and not digested well- LacI transformed and digested (10/08/09) | ?? | BBa_???? |
CC | (Pbad :: rbs - lacI - LVA) :: ter in [http://partsregistry.org/Part:pSB1A3 pSB1A3] | BBa_??? :: [http://partsregistry.org/Part:BBa_B0014 BBa_B0014] in [http://partsregistry.org/Part:pSB2K3 pSB2K3] | ter transformed, digested and purified (10/08/09) | ?? | BBa_???? |
CC | ((Pbad :: rbs - lacI - LVA) :: RFP) in [http://partsregistry.org/Part:pSB1A3 pSB1A3] | BBa_??? :: [http://partsregistry.org/wiki/index.php?title=Part:BBa_K093005 BBa_K093005] in [http://partsregistry.org/Part:pSB2K3 pSB2K3] | pSB2K3 transformed and digested, RFP : primers designed but not yet ordered (10/08/09) | ?? | BBa_????
|
CC | (((Pbad :: rbs - lacI - LVA) :: RFP) :: ter) in [http://partsregistry.org/Part:pSB1A3 pSB1A3] | BBa_??? :: [http://partsregistry.org/Part:BBa_B0014 BBa_B0014] in [http://partsregistry.org/Part:pSB2K3 pSB2K3] | ?? | BBa_????
| |
CC | (OmpA Linker :: g3p) in [http://partsregistry.org/Part:pSB2K3 pSB2K3] | [http://partsregistry.org/Part:BBa_K103006 BBa_K103006] :: BBa_??? in [http://partsregistry.org/Part:pSB2K3 pSB2K3] | OmpA linker standardisé - OmpA amplified, not purified - Same for g3p (10/08/09) | ?? | BBa_???? |
(g3p :: clyA) in [http://partsregistry.org/Part:pSB2K3 pSB2K3] | BBa_??? :: BBa_??? in [http://partsregistry.org/Part:pSB2K3 pSB2K3] | autre possibilité (souf)- 1 primer missing for C term fusion with clyA (10/08/09) | ?? | BBa_???? | |
CC! | ((OmpA Linker :: g3p) :: ter) in [http://partsregistry.org/Part:pSB2K3 pSB2K3] | BBa_??? :: [http://partsregistry.org/Part:BBa_B0014 BBa_B0014] in [http://partsregistry.org/Part:pSB2K3 pSB2K3] | ?? | BBa_???? | |
CC! | ((???autotransporter??? - jun/foss) :: ter) in [http://partsregistry.org/Part:pSB2K3 pSB2K3] | BBa_??? :: [http://partsregistry.org/Part:BBa_B0014 BBa_B0014] in [http://partsregistry.org/Part:pSB2K3 pSB2K3] | jun/foss has to be synthetized & autotransporter to be found!!!! (10/08/09) | ?? | BBa_???? |
CC! | ((rbs - fecA) :: ((rbs - jun/foss) :: ter)) in [http://partsregistry.org/Part:pSB2K3 pSB2K3] | BBa_??? :: BBa_??? in [http://partsregistry.org/Part:pSB2K3 pSB2K3] | FecA : oligo ordered but not received yet (10/08/09) | ?? | BBa_???? |
CC! | ((rbs - fecA) :: ((Ompa Linker :: g3p) :: ter)) in [http://partsregistry.org/Part:pSB2K3 pSB2K3] | BBa_??? :: BBa_??? in [http://partsregistry.org/Part:pSB2K3 pSB2K3] | ?? | BBa_???? | |
CC | ((rbs - ClyA :: RFP) in [http://partsregistry.org/Part:pSB2K3 pSB2K3] | BBa_??? :: BBa_???? in [http://partsregistry.org/Part:pSB2K3 pSB2K3] | modif RFP (souf)- some troubles with amplification of clyA fusion C term (10/08/09) | ?? | BBa_???? |
CC | ((rbs-RFP :: clyA) in [http://partsregistry.org/Part:pSB2K3 pSB2K3] | BBa_??? :: BBa_???? in [http://partsregistry.org/Part:pSB2K3 pSB2K3] | modif RFP (souf) - fusion N term de cly A (autre possibilité)- we still miss 1 primer for clyA N term fusion (10/08/09) | ?? | BBa_???? |
CC! | ((rbs - ClyA :: RFP)) :: (((rbs - fecA) :: (rbs - jun/foss)) :: ter)) in [http://partsregistry.org/Part:pSB2K3 pSB2K3] | BBa_??? :: BBa_???? in [http://partsregistry.org/Part:pSB2K3 pSB2K3] | modif RFP (souf) | ?? | BBa_???? |
CC! | ((rbs - ClyA :: RFP) :: (((rbs - fecA) :: (OmpA linker :: g3p)) :: ter)) in [http://partsregistry.org/Part:pSB2K3 pSB2K3] | BBa_??? :: BBa_???? in [http://partsregistry.org/Part:pSB2K3 pSB2K3] | modif RFP (souf) | ?? | BBa_???? |
CC! | (Pbad :: ((rbs - ClyA :: RFP) :: (((rbs - fecA) :: (OmpA linker :: g3p)) :: ter))) in [http://partsregistry.org/Part:pSB2K3 pSB2K3] | [http://partsregistry.org/Part:BBa_I0500 Bba_I0500] :: BBa_???? in [http://partsregistry.org/Part:pSB2K3 pSB2K3] | ?? | BBa_???? | |
CC! | (Pbad :: ((rbs - ClyA :: RFP) :: (((rbs - fecA) :: (rbs - jun/foss)) :: ter))) in [http://partsregistry.org/Part:pSB2K3 pSB2K3] | BBa_??? :: BBa_???? in [http://partsregistry.org/Part:pSB2K3 pSB2K3] | ?? | BBa_???? | |
CC! | ((Pbad :: ((rbs - ClyA :: RFP) :: (((rbs - fecA) :: (OmpA linker :: g3p)) :: ter))) :: ((Pbad :: rbs - lacI - LVA) :: ter)) in [http://partsregistry.org/Part:pSB2K3 pSB2K3] | BBa_??? :: BBa_???? in [http://partsregistry.org/Part:pSB2K3 pSB2K3] | 2*Pbad et 2*ter?? (souf) | ?? | BBa_???? |
CC! | ((Pbad :: ((rbs - ClyA :: RFP) :: (((rbs - fecA) :: (rbs - jun/foss)) :: ter))) :: ((Pbad :: rbs - lacI - LVA) :: ter)) in [http://partsregistry.org/Part:pSB2K3 pSB2K3] | BBa_??? :: BBa_???? in [http://partsregistry.org/Part:pSB2K3 pSB2K3] | ?? | BBa_????
|
- Plasmid B
Designer | Parts description | Corresponding BioBricks | Status (last update) | Plasmid # | Registry |
GC | ((Plac :: rbs-tetR) :: rbs-tolRII) in pSB2A3 | BBa_blabla :: BBa_tralala in truc | Planning / oligo desgned or ordered / PCR in progress / cloned / sequencing... (10/08/09) | 12 | BBa_pouet pouet |
CC | (Plac :: rbs-tetR) in [http://partsregistry.org/Part:pSB1A3 pSB1A3] | [http://partsregistry.org/Part:BBa_I14032 Bba_I14032] :: [http://partsregistry.org/Part:BBa_K136050 BBa_K136050] in [http://partsregistry.org/Part:pSB1A3 pSB1A3] | both have been digested and purified, ligation in progress (10/08/09) | ?? | BBa_???? |
CC | ((Plac :: rbs-tetR) :: ter) in [http://partsregistry.org/Part:pSB1A3 pSB1A3] | BBa_???? :: [http://partsregistry.org/Part:BBa_B0014 BBa_B0014] in [http://partsregistry.org/Part:pSB1A3 pSB1A3] | ?? | [http://partsregistry.org/wiki/index.php?title=Part:BBa_K257015 BBa_K257015] | |
CC | (ter :: ((Plac :: rbs-tetR) :: ter)) in [http://partsregistry.org/Part:pSB1A3 pSB1A3] | [http://partsregistry.org/Part:BBa_B0014 BBa_B0014] :: [http://partsregistry.org/wiki/index.php?title=Part:BBa_K257015 BBa_K257015] in [http://partsregistry.org/Part:pSB1A3 pSB1A3] | ?? | BBa_???? | |
CL | (rbs - OmpAsignal :: TolR II) | [http://partsregistry.org/wiki/index.php?title=Part:BBa_K257006 BBa_K257006] ::[http://partsregistry.org/wiki/index.php?title=Part:BBa_K257005 BBa_K257005] in [http://partsregistry.org/Part:pSB1A3 pSB1A3] | OmpA signal and TolRII amplified, but neither digested nor purified (10/08/09) | ?? | BBa_???? |
CC | (Ptet :: (rbs - OmpAsignal :: TolRII)) in [http://partsregistry.org/Part:pSB1A3 pSB1A3] | [http://partsregistry.org/Part:BBa_R0040 Bba_R0040] :: [http://partsregistry.org/wiki/index.php?title=Part:BBa_K257006 BBa_K257006] ::[http://partsregistry.org/wiki/index.php?title=Part:BBa_K257005 BBa_K257005] in [http://partsregistry.org/Part:pSB1A3 pSB1A3] | Ptet digested and purified (10/08/09) | ?? | BBa_???? |
CC | (rbs :: EYFP) in [http://partsregistry.org/Part:pSB1A3 pSB1A3] | BBa_??? :: [http://partsregistry.org/wiki/index.php?title=Part:BBa_E0030 BBa_E0030] in [http://partsregistry.org/Part:pSB1A3 pSB1A3] | y a t-il vraiment 2 biobricks ou bien met-on le RBS direct dans des oligos pour RFP? (souf) biobrick to find or primers to design (10/08/09) | ?? | BBa_???? |
CC | (Ptet :: (rbs :: EYFP)) in [http://partsregistry.org/Part:pSB1A3 pSB1A3] | [http://partsregistry.org/Part:BBa_R0040 Bba_R0040] :: BBa_??? in [http://partsregistry.org/Part:pSB1A3 pSB1A3] | ?? | BBa_???? | |
CC | ((Ptet :: (rbs :: EYFP)) :: (ter :: ((Plac :: rbs-tetR) :: ter))) in [http://partsregistry.org/Part:pSB1A3 pSB1A3] | BBa_??? :: BBa_??? in [http://partsregistry.org/Part:pSB1A3 pSB1A3] | ?? | BBa_???? | |
CC | ((Ptet :: (rbs - OmpAsignal :: TolRIIP)) :: (ter :: ((Plac :: rbs-tetR) :: ter))) in [http://partsregistry.org/Part:pSB1A3 pSB1A3] | BBa_??? :: BBa_??? in [http://partsregistry.org/Part:pSB1A3 pSB1A3] | ?? | BBa_???? |
- Plasmid C
Designer | Parts description | Corresponding BioBricks | Status (last update) | Plasmid # | Registry |
CC | (rbs-FecI :: rbs-FecR) in [http://partsregistry.org/Part:pSB2K3 pSB2K3] | BBa_??? :: BBa_??? | primers ordered but not received yet (10/08/09) | ?? | BBa_????
|
CC | PfecA in [http://partsregistry.org/Part:BBa_J61002 Bba_J61002] | BBa_??? | vector digested and purified, Pfec A amplified but not digested (purified?) (10/08/09) | ?? | BBa_???? |