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Team:Warsaw/Calendar-Main/23 August 2009
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(New page: {{WarNotebook}} <!-- do not edit above me! --> <html> <h3><div style="text-align: center;">Assembly of endosomal detection operon</div></h3> <h4>Marcin</h4> <br /> <p>Task 1:</p><ul> <li>...) |
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<li>Digest was performed one hour</li> | <li>Digest was performed one hour</li> | ||
<p>Results:</p> | <p>Results:</p> | ||
| - | <p>All isolated samples have expected plasmid</p> | + | <img src="https://static.igem.org/mediawiki/2009/5/56/R0080_C0040%2BRBS_23_08_09.png" width="45%" heigth="45%"> |
| - | </ | + | <font face="Times New Roman" size="3"><p><div style="text-align: center;">All isolated samples have expected plasmid</div></p></font> |
| + | <h3><div style="text-align: center;">Making of the plac-RBS-llo-intA part</div></h3> | ||
| + | <h4>Jarek</h4> | ||
| + | <br /> | ||
| + | <p>Tasks:</p> | ||
| + | <ul> | ||
| + | <li>Transformating the competent ''E.coli'' strain TopF' with ligation</li> | ||
| + | <li>Plating the transformated culure on LB+Amp plates</li> | ||
| + | </ul> | ||
| + | <br /> | ||
| + | <h3><div style="text-align: center;">Cloning switch 1 regulatory parts [ | ||
| + | <a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K177012">K177012</a>-PcI.RBS.LacI, | ||
| + | <a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K177033">K177033</a>-PcI.RBS.LacI.PcI.RBS.RFP.terminator, | ||
| + | <a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K177011">K177011</a>-PLacI.RBS.cI.terminator, | ||
| + | <a href="http://partsregistry.org/Part:BBa_K177038">K177038</a>-PLacI.RBS.cI.terminator.PLacI.RBS.GFP.terminator | ||
| + | ] | ||
| + | into two compatible low copy number plasmids of different antibiotic resistance</div></h3> | ||
| + | <h4>Ania</h4> | ||
| + | <br/> | ||
| + | <p>Tasks:</p> | ||
| + | <ul> | ||
| + | <li> | ||
| + | </li> | ||
| + | </ul> | ||
| + | <br/> | ||
| + | </html> | ||
<!-- do not remove this! --> | <!-- do not remove this! --> | ||
{{WarNotebookEnd}} | {{WarNotebookEnd}} | ||
Latest revision as of 05:22, 14 September 2009
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Assembly of endosomal detection operon
Marcin
Task 1:
- Transformation of chemocompetent E. coli strain DH5&alpha
Constructs to transform:
Methods:
- Detailed protocol of transformation is described here.
Task 2:
- Isolate of plasmids from bacterial cultures prepared in 22.08.09
Methods:
- Plasmids were isolated using the A&A plasmid mini kit. Detailed procedure of the isolation is described here
Task 3:
- Digest previously isolated plasmids and verify the correctness of the ligation:
Methods:
- Reaction mixture composition:
2 μl purified plasmid DNA product 0.5 μl XbaI (Fermentas) 0.5 μl PstI (Fermentas) 2 μl Buffer Tango (Fermentas) 15 μl MQ water
Results:
All isolated samples have expected plasmid
Making of the plac-RBS-llo-intA part
Jarek
Tasks:
- Transformating the competent ''E.coli'' strain TopF' with ligation
- Plating the transformated culure on LB+Amp plates
Ania
Tasks:
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