Team:Warsaw/Calendar-Main/23 August 2009
From 2009.igem.org
Assembly of endosomal detection operon
Marcin
Task 1:
- Transformation of chemocompetent E. coli strain DH5&alpha
Constructs to transform:
Methods:
- Detailed protocol of transformation is described here.
Task 2:
- Isolate of plasmids from bacterial cultures prepared in 22.08.09
Methods:
- Plasmids were isolated using the A&A plasmid mini kit. Detailed procedure of the isolation is described here
Task 3:
- Digest previously isolated plasmids and verify the correctness of the ligation:
Methods:
- Reaction mixture composition:
2 μl purified plasmid DNA product 0.5 μl XbaI (Fermentas) 0.5 μl PstI (Fermentas) 2 μl Buffer Tango (Fermentas) 15 μl MQ water
Results:
All isolated samples have expected plasmid
Making of the plac-RBS-llo-intA part
Jarek
Tasks:
- Transformating the competent ''E.coli'' strain TopF' with ligation
- Plating the transformated culure on LB+Amp plates
Ania
Tasks:
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