Team:UNICAMP-Brazil/Notebooks/September 1

(Difference between revisions)

Latest revision as of 02:01, 22 October 2009

We decided to make cDNA synthesis of the 4 samples of RNA (Blood1, Blood3, Spleen1 and Oviduct2). We used both pDN6 and oligodT primers for the synthesis.

Taís

We performed a new digestion of the plasmids containing the ligation RBS+CFP. We followed Protocol 14. The digestion lasted four hours and we used less plasmid comparing to the first digestion.

Wesley

@@ Line 8: / Line 8: @@
 ====''Gallus gallus'' cDNA synthesis====
-We decided to make cDNA synthesis of the 4 samples of RNA (Blood1, Blood3, Spleen1 and Oviduct2). We used both pDN6 and oligodT primers for the synthesis.
+*<p style=”text-align:justify;”>We decided to make cDNA synthesis of the 4 samples of RNA (Blood1, Blood3, Spleen1 and Oviduct2). We used both pDN6 and oligodT primers for the synthesis.</p>
 ''Taís''
-====RBS+CFP ligation====
+====RBS+CFP Digestion====
-We performed a new digestion of the plasmids containing the ligation RBS+CFP. We followed Protocol Y (see protocols section). The digestion lasted four hours and we used less plasmid comparing to the first digestion.
+*<p style=”text-align:justify;”>We performed a new digestion of the plasmids containing the ligation RBS+CFP. We followed [https://2009.igem.org/Team:UNICAMP-Brazil/Protocols/Restriction_reaction Protocol 14]. The digestion lasted four hours and we used less plasmid comparing to the first digestion.</p>
 ''Wesley''
 {{:Team:UNICAMP-Brazil/inc_rodape}}