Team:UNICAMP-Brazil/Notebooks/September 6
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''Marcelo'' | ''Marcelo'' | ||
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+ | ==== Cre Recombinase ==== | ||
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+ | *<p style=”text-align:justify;”>Today we performed minipreps of Cre-Recombinase´s biobrick, that we confirmed by running an electrophoresis agarose gel.</p> | ||
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+ | ''Víctor'' | ||
==''' YeastGuard '''== | ==''' YeastGuard '''== | ||
====New biobricks==== | ====New biobricks==== | ||
- | Today we digested all our 4 new biobrick parts with both enzymes: ''Xba''I and ''Spe''I (Promoter of JEN1 from ''K. lactis''; Promoter of DLD1 from ''K. lactis''; Lysozyme from ''G. gallus''; Gene JEN1 from ''K. lactis''). We also digested the biofusion vector with the same enzymes for future ligation. | + | *<p style=”text-align:justify;”>Today we digested all our 4 new biobrick parts with both enzymes: ''Xba''I and ''Spe''I (Promoter of JEN1 from ''K. lactis''; Promoter of DLD1 from ''K. lactis''; Lysozyme from ''G. gallus''; Gene JEN1 from ''K. lactis''). We also digested the biofusion vector with the same enzymes for future ligation.</p> |
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+ | [[Image:20090905_PCR_partes_BioF.png|300px|center|]] | ||
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* Digestions that worked: | * Digestions that worked: | ||
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- Promoter of DLD1 from ''K. lactis'' | - Promoter of DLD1 from ''K. lactis'' | ||
- | - | + | - Biofusion vector |
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* Digestions that didnt work: | * Digestions that didnt work: | ||
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- Gene JEN1 from ''K. lactis'' | - Gene JEN1 from ''K. lactis'' | ||
- | - | + | - Lysozyme from ''G. gallus'' |
{{:Team:UNICAMP-Brazil/inc_rodape}} | {{:Team:UNICAMP-Brazil/inc_rodape}} |
Latest revision as of 02:12, 22 October 2009
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