Team:Paris/Addressing overview strategy

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== Overview ==
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* [[Team:Paris/Addressing_overview_exportsystem#Overview |A. Export system ]]
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* [[Team:Paris/Addressing_overview_strategy#Overview |B. Our strategy]]
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==Our strategy==
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* [[Team:Paris/Addressing_overview_Construction#Overview |C. Construction]]
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<a class="menu_sub" href="https://2009.igem.org/Team:Paris/Addressing_overview_exportsystem#bottom"> Export system</a>|
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<a class="menu_sub_active"href="https://2009.igem.org/Team:Paris/Addressing_overview_strategy#bottom"> Our Strategy</a>|
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<a class="menu_sub"href="https://2009.igem.org/Team:Paris/Addressing_testing#bottom"> Construction</a>
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==B. Our strategy==
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We thought of overexpressing the important proteins in the Tat pathway (that is to say TatABCE) in order to avoid the early saturation phenomenon that is likely to occur when we will overexpress proteins that will be targeted to the outer membrane or to the periplasm.
We thought of overexpressing the important proteins in the Tat pathway (that is to say TatABCE) in order to avoid the early saturation phenomenon that is likely to occur when we will overexpress proteins that will be targeted to the outer membrane or to the periplasm.
However, in our strategy, the only protein that needs to use the TAT pathway to translocate from the cytoplasm to the periplasm is clyA. Finally, we decided that the overexpression won't be necessary neither for the TAT pathway, nor for the SEC pathway, both of them constitutively expressed in E Coli K12.
However, in our strategy, the only protein that needs to use the TAT pathway to translocate from the cytoplasm to the periplasm is clyA. Finally, we decided that the overexpression won't be necessary neither for the TAT pathway, nor for the SEC pathway, both of them constitutively expressed in E Coli K12.

Revision as of 01:10, 21 October 2009

iGEM > Paris > Adressing > Export systems > Our strategy


Our strategy


We thought of overexpressing the important proteins in the Tat pathway (that is to say TatABCE) in order to avoid the early saturation phenomenon that is likely to occur when we will overexpress proteins that will be targeted to the outer membrane or to the periplasm.

However, in our strategy, the only protein that needs to use the TAT pathway to translocate from the cytoplasm to the periplasm is clyA. Finally, we decided that the overexpression won't be necessary neither for the TAT pathway, nor for the SEC pathway, both of them constitutively expressed in E Coli K12.

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