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Team:UNICAMP-Brazil/Notebooks/September 16
From 2009.igem.org
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*<p style=”text-align:justify;”>The digestion with the ''HindIII'' restriction enzyme was performed according to Protocol X. In place of the second enzyme that is used in this reaction, it was used the same volume of autoclaved distilled water.</p> | *<p style=”text-align:justify;”>The digestion with the ''HindIII'' restriction enzyme was performed according to Protocol X. In place of the second enzyme that is used in this reaction, it was used the same volume of autoclaved distilled water.</p> | ||
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''Gabriel'' | ''Gabriel'' | ||
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==== PY Promoter - Transformation ==== | ==== PY Promoter - Transformation ==== | ||
| - | *<p style=”text-align:justify;”>After the O/N period we transformed the ligations PY1 + BBa_J23100 and PY2 + BBa_J23100 into electrocompetent ''E. coli'' bacteria, strain DH10B. We followed [https://2009.igem.org/Team:UNICAMP-Brazil/Protocols/Electroporation Protocol 3] (Electroporation) without modifications. | + | *<p style=”text-align:justify;”>After the O/N period we transformed the ligations PY1 + BBa_J23100 and PY2 + BBa_J23100 into electrocompetent ''E. coli'' bacteria, strain DH10B. We followed [https://2009.igem.org/Team:UNICAMP-Brazil/Protocols/Electroporation Protocol 3] (Electroporation) without modifications.</p> |
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| + | *<p style=”text-align:justify;”>After the transformation we plated the transformed cells in LB-AMP plates and let them grow at 37ºC for an O/N period.</p> | ||
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| - | + | ''Fabiana and Leonardo'' | |
{{:Team:UNICAMP-Brazil/inc_rodape}} | {{:Team:UNICAMP-Brazil/inc_rodape}} | ||
Revision as of 14:02, 19 October 2009
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