Team:Paris/Production testing

From 2009.igem.org

(Difference between revisions)
(→Part II : Testing delay phase II)
(→The destabilization of the membrane)
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|- style="background: #bebebe; text-align: center;"
|- style="background: #bebebe; text-align: center;"
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|'''PCR :'''  
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|bgcolor="#F8F8F8"|'''PCR :'''  
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|'''PCR :'''
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TatABCE matrix :
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|'''PCR :'''
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|bgcolor="#E0E3FE"|'''PCR :'''
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|bgcolor="#CBD1FD"|'''PCR :'''
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|bgcolor="#BCCDFD"|'''PCR :'''
|- style="background: #d8d8d8; text-align: center;"
|- style="background: #d8d8d8; text-align: center;"
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|'''Verification on gel :'''
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|bgcolor="#F8F8F8"|'''Verification on gel :'''
ok
ok
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|'''Verification on gel :'''
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|bgcolor="#E0E3FE"|'''Verification on gel :'''
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ok
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-
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|'''Verification on gel :'''
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|bgcolor="#CBD1FD"|'''Verification on gel :'''
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ok
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|bgcolor="#BCCDFD"|'''Verification on gel :'''
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|- style="background: #bebebe; text-align: center;"   
|- style="background: #bebebe; text-align: center;"   
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|'''Purification on gel :'''
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|bgcolor="#F8F8F8"|'''Purification on gel :'''
ok
ok
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|'''Purification on gel :'''
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|bgcolor="#E0E3FE"|'''Purification on gel :'''
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|'''Purification on gel :'''
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|bgcolor="#CBD1FD"|'''Purification on gel :'''
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|bgcolor="#BCCDFD"|'''Purification on gel :'''
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|- style="background: #d8d8d8; text-align: center;"
 
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|- style="background: #d8d8d8; text-align: center;"
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TatABCE
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|bgcolor="#E0E3FE"|'''digestion:'''
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|bgcolor="#CBD1FD"|'''digestion:'''  
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|bgcolor="#BCCDFD"|'''digestion:'''  
|- style="background: #bebebe; text-align: center;"   
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|bgcolor="#F8F8F8"|'''verification digestion:'''
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|- style="background: #d8d8d8; text-align: center;"
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TatABCE
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|bgcolor="#BCCDFD"|'''ligation:'''
|- style="background: #bebebe; text-align: center;"   
|- style="background: #bebebe; text-align: center;"   
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|bgcolor="#F8F8F8"|'''PCR colo :'''
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|bgcolor="#BCCDFD"|'''PCR colo :'''
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|- style="background: #d8d8d8; text-align: center;"
|- style="background: #d8d8d8; text-align: center;"
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|'''miniprep:'''
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|bgcolor="#F8F8F8"|'''miniprep:'''
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clone :
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STOPPED
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clone :
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clone :
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|bgcolor="#BCCDFD"|'''miniprep:'''
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|- style="background: #bebebe; text-align: center;"   
|- style="background: #bebebe; text-align: center;"   
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|'''sequencing :'''
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|bgcolor="#F8F8F8"|'''sequencing :'''
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ok
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|'''sequencing :'''
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|bgcolor="#E0E3FE"|'''sequencing :'''
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|'''sequencing :'''
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|bgcolor="#CBD1FD"|'''sequencing :'''
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Revision as of 18:40, 21 October 2009

iGEM > Paris > WetLab > Production


Contents

WetLab - Production

  1. The delay
    1. Part I : Testing delay phase I
    2. Part I : Functional testing of the part I
    3. Part II : Testing delay phase II
    4. Part II : Functional testing of the part II
  2. The destabilization of the membrane

The delay

We separate the construction on two plasmids : Part I and Part II.

Part I : Testing delay phase I

plasmid = PSB3T5



Time required : A lOT !!!!!

Experiments ran :


column 1 column 2 column 3 column 4


PCR :

TatABCE matrix :

PCR : PCR : PCR :
Verification on gel :

ok

Verification on gel :

-

Verification on gel :

-

Verification on gel :

-

Purification on gel :

ok

Purification on gel :

-

Purification on gel :

-

Purification on gel :

-


digestion:

TatABCE

digestion: digestion: digestion:
verification digestion:

ok

verification digestion:

-

verification digestion:

-

verification digestion:

-


ligation:

TatABCE

ligation: ligation: ligation:
PCR colo :

ok

PCR colo :

-

PCR colo :

-

PCR colo :

-

miniprep:

STOPPED

miniprep:

-

miniprep:

-

miniprep:

-

sequencing :

-

sequencing :

-

sequencing :

-

sequencing :

-

Part I : Functional testing of the part I

PBAD LacI LVA- YFP was transforme into Top ten Bacteria and grew on medium :


1% arabinose

1% glucose

LB pure


Part II : Testing delay phase II

plasmid =


Time required : A lOT !!!!!

Experiments ran :

column 1 column 2 column 3 column 4


PCR :

TatABCE matrix :

PCR : PCR : PCR :
Verification on gel :

ok

Verification on gel :

-

Verification on gel :

-

Verification on gel :

-

Purification on gel :

ok

Purification on gel :

-

Purification on gel :

-

Purification on gel :

-


digestion:

TatABCE

digestion: digestion: digestion:
verification digestion:

ok

verification digestion:

-

verification digestion:

-

verification digestion:

-


ligation:

TatABCE

ligation: ligation: ligation:
PCR colo :

ok

PCR colo :

-

PCR colo :

-

PCR colo :

-

miniprep:

STOPPED

miniprep:

-

miniprep:

-

miniprep:

-

sequencing :

-

sequencing :

-

sequencing :

-

sequencing :

-

Part II : Functional testing of the part II

Ptet RFP Plac TetR was tranforme into Top10 bacteria and grew on media :


0,1% IPTG .

1% glucose.

LB pure.



The destabilization of the membrane

plasmid = PSB3T5






Time required : A lOT !!!!!


Experiments ran :

column 1 column 2 column 3 column 4


PCR :

TatABCE matrix :

PCR : PCR : PCR :
Verification on gel :

ok

Verification on gel :

-

Verification on gel :

-

Verification on gel :

-

Purification on gel :

ok

Purification on gel :

-

Purification on gel :

-

Purification on gel :

-


digestion:

TatABCE

digestion: digestion: digestion:
verification digestion:

ok

verification digestion:

-

verification digestion:

-

verification digestion:

-


ligation:

TatABCE

ligation: ligation: ligation:
PCR colo :

ok

PCR colo :

-

PCR colo :

-

PCR colo :

-

miniprep:

STOPPED

miniprep:

-

miniprep:

-

miniprep:

-

sequencing :

-

sequencing :

-

sequencing :

-

sequencing :

-



Retrieved from "http://2009.igem.org/Team:Paris/Production_testing"