Team:UNICAMP-Brazil/Notebooks/October 18

From 2009.igem.org

(Difference between revisions)
(→PY Promoter - Colony-PCR)
(→Yeast experiments)
 
Line 27: Line 27:
====Yeast experiments====
====Yeast experiments====
-
*<p style=”text-align:justify;”>We did miniprep of 10 inocula of pDLD+YFP and 10 inocula of Adh1+YFP. We transformed the plasmids in competent yeast. Hope to find colonies tomorrow! =)</p>
+
*<p style=”text-align:justify;”>We did miniprep of 10 inocula of pDLD+YFP and 10 inocula of ''ADH1''+YFP. We transformed the plasmids in competent yeast. Hope to find colonies tomorrow! =)</p>
''Raíssa and Taís''
''Raíssa and Taís''
{{:Team:UNICAMP-Brazil/inc_rodape}}
{{:Team:UNICAMP-Brazil/inc_rodape}}

Latest revision as of 03:38, 22 October 2009

Topo l2.gif topo_r_igem.gif
topo_r_b.gif
Back to Calendar

ColiGuard

PY Promoter - Colony-PCR

  • We got one single colony from the transformation we did yesterday!

  • We performed a colony-pcr to confirm that this colony has our plasmid.

  • We used 2 pairs of primers - VF/VR and PY primers - and analyzed the results of our PCR in an agarose gel:

Py gel 9.png

  • The expected size for PY1 + RFP + BBa_B0015 amplified with VF/VR primers is 1279 bp.

  • The expected size for PY1 + RFP + BBa_B0015 amplified with PY primers is 133 bp.

  • As we can observe in the gel photo, the colony presented bands compatible with the size expected for both pairs of primers!!!! =)

Fabi and Léo

YeastGuard

Yeast experiments

  • We did miniprep of 10 inocula of pDLD+YFP and 10 inocula of ADH1+YFP. We transformed the plasmids in competent yeast. Hope to find colonies tomorrow! =)

Raíssa and Taís




Retrieved from "http://2009.igem.org/Team:UNICAMP-Brazil/Notebooks/October_18"