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Team:Paris/Protocols PCRqload
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==PCR quick load Taq== | ==PCR quick load Taq== | ||
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| + | <p>Préparation de la PCR avec un Vf=50µL:</p> | ||
| + | <p>-1µL Matrice</p> | ||
| + | <p>-14µL H2O</p> | ||
| + | <p>-5µ Oligo Fw (à 0,2M)</p> | ||
| + | <p>-5µL Oligo Rw (à 0,2M)</p> | ||
| + | <p>-25µL Tampon+Taq (Taq quick load master mix 2X)</p> | ||
Revision as of 08:11, 28 July 2009
PCR quick load Taq
Préparation de la PCR avec un Vf=50µL:
-1µL Matrice
-14µL H2O
-5µ Oligo Fw (à 0,2M)
-5µL Oligo Rw (à 0,2M)
-25µL Tampon+Taq (Taq quick load master mix 2X)
