EPF-Lausanne/10 August 2009

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(Wet Lab)
 
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==Wet Lab==
==Wet Lab==
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We test a ligation with LacI-RBS without Phosphatase and with different concentration of vector and insert. We also test to digest a GFP plasmid and ligase it without insert.
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We tested a ligation with LacI-RBS without Phosphatase and with different concentrations of vector and insert. We also tried ligating a digested GFP plasmid. The ligations were left overnight.
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We also made liquid cultures of RBS, GFP, LacI, backbones 1 and 2 (resistances to kanamycin and chlorophenicol).
==People in the lab==
==People in the lab==

Latest revision as of 07:35, 11 August 2009

Contents

10 August 2009





Wet Lab

We tested a ligation with LacI-RBS without Phosphatase and with different concentrations of vector and insert. We also tried ligating a digested GFP plasmid. The ligations were left overnight.

We also made liquid cultures of RBS, GFP, LacI, backbones 1 and 2 (resistances to kanamycin and chlorophenicol).

People in the lab

Nath, Basile, Gab, Christian, Nicolas