Team:Paris/Transduction overview transduction

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* [[Team:Paris/Transduction_overview_strategy#bottom |C. Our strategy]]
* [[Team:Paris/Transduction_overview_strategy#bottom |C. Our strategy]]
* [[Team:Paris/Transduction_overview_construction#bottom |D. Construction]]
* [[Team:Paris/Transduction_overview_construction#bottom |D. Construction]]
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===B. Transduction===
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====B.1 ABC transporter====
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[[Image:Tranduction_abc_transporter_b12.png|380px|right| fec operon induction]]
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The ABC transporter is a major class of cellular translocation machinery  encoded in the largest set of paralogous genes.
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The ABC (ATP binding cassette) transporter is one of the active transport systems of the cell,
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which is widespread in archaea, eubacteria, and eukaryotes. It is also known as the
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periplasmic binding protein-dependent transport system in Gram-negative bacteria and the binding-
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lipoprotein-dependent transport system in Gram positive bacteria. The transporter shows a common
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global organization with three types of molecular components. Typically, it consists of two integral membrane proteins (permeases)
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each having six transmembrane segments, two peripheral membrane proteins that bind and hydrolyze ATP,
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and a periplasmic (or lipoprotein) substrate-binding protein. The ATP-binding protein component is the most
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conserved, the membrane protein component is somewhat less conserved, and the substrate-binding protein
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component is most divergent in terms of the sequence similarity. The ABC transporters form the largest group of paralogous genes in bacterial
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and archaeal genomes , and the genes for the three components frequently form an operon.
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=====B.1.1 Uses=====
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transport the protein directly into the cytolpasm to activate the transcription.
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=====B.1.2 Advantages/drawbacks=====
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advantages:
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the protein of interest is directly translocated in the cytoplasm and if it is a transcription factor it could activated immediatly the response.
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drawbacks:
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It is a nutriment uptake system, so basically only small molecules are able to pass throught the membranes and it is a very specific.
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Revision as of 20:05, 2 September 2009

iGEM > Paris > Reception > Transduction

Contents

Overview

B. Transduction

B.1 ABC transporter

fec operon induction

The ABC transporter is a major class of cellular translocation machinery encoded in the largest set of paralogous genes.


The ABC (ATP binding cassette) transporter is one of the active transport systems of the cell, which is widespread in archaea, eubacteria, and eukaryotes. It is also known as the periplasmic binding protein-dependent transport system in Gram-negative bacteria and the binding- lipoprotein-dependent transport system in Gram positive bacteria. The transporter shows a common global organization with three types of molecular components. Typically, it consists of two integral membrane proteins (permeases) each having six transmembrane segments, two peripheral membrane proteins that bind and hydrolyze ATP, and a periplasmic (or lipoprotein) substrate-binding protein. The ATP-binding protein component is the most conserved, the membrane protein component is somewhat less conserved, and the substrate-binding protein component is most divergent in terms of the sequence similarity. The ABC transporters form the largest group of paralogous genes in bacterial and archaeal genomes , and the genes for the three components frequently form an operon.

B.1.1 Uses

transport the protein directly into the cytolpasm to activate the transcription.

B.1.2 Advantages/drawbacks

advantages:

the protein of interest is directly translocated in the cytoplasm and if it is a transcription factor it could activated immediatly the response.


drawbacks:

It is a nutriment uptake system, so basically only small molecules are able to pass throught the membranes and it is a very specific.


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