Team:UNICAMP-Brazil/Notebooks/August 31

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Revision as of 20:17, 7 September 2009

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YeastGuard

Gallus gallus RNA

Quantification

We have verified the concentration of RNA we got in the RNA extractions from the G. gallus organs. The following table shows the results.

Samples	ng/uL	260/280	260/230
Blood 1	743.42	2.01	1.78
Blood 2	604.84	1.99	1.54
Blood 3	1814.10	1.97	1.88
Spleen 1	3607.90	1.96	1.57
Spleen 2	3715.95	1.94	1.55
Spleen 3	4213.93	1.78	1.69
Spleen 4	4243.37	1.76	1.69
Oviduct 1	4362.04	1.66	1.41
Oviduct 2	3498.69	1.87	1.16
Oviduct 3	3601.43	1.84	1.13
Oviduct 4	3568.66	1.84	1.12

The concentration values are ok, but the ratios 260/280 and 260/230 are not that good. Let’s find out what we see in the agarose gel.

Raíssa

Agarose gel

We have chosen the best samples from each organ to verify the quality in the gel. The samples are: Blood1, Blood3, Spleen1 and Oviduct2. We had problems with our UV transilluminator and didn’t manage to take pictures of the gel. =[ But we are going to tell you what we found! The bands were there, especially in the spleen and oviduct samples, not perfect, but we bet they are ok for cDNA synthesis.

Taís

Terminator biobrick and RBS+CFP ligation

We verified using electrophoresis, the digestion products of both parts digested yesterday (Terminator and RBS+CFP). The gel showed we had no success with the digestions. Two not specific bands appeared.

Wesley

@@ Line 47: / Line 47: @@
 ''Taís''
-====Terminator and RBS+CFP====
+====Terminator biobrick and RBS+CFP ligation====
-Electrophoresis of the digestion products of both parts digested yesterday (Terminator and RBS+CFP)
+We verified using electrophoresis, the digestion products of both parts digested yesterday (Terminator and RBS+CFP).
 The gel showed we had no success with the digestions. Two not specific bands appeared.